Standard name | |
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Human Ortholog | |
Description | Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs); interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 70 | 83 | 99 | 125 | 254 | 313 | 348 | 346 | 179 | 216 | 164 | 183 | – | 24 | 107 | 115 | 91 | 113 | 81 | 78 | 87 | 21 | 28 | 59 |
Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 1 | 1 | 6 | 7 | 5 | 4 | 2 | 2 | – | 1 | 1 | 4 | 6 | 5 | 0 | 0 | 1 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 2 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 1 | 0 | 1 | 1 | 2 | 2 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | – | 0 | 0 | 1 | 0 | 1 | 2 | 4 | 0 | 1 | 0 | 1 |
Unique Cell Count | 71 | 86 | 101 | 125 | 255 | 316 | 355 | 352 | 183 | 220 | 167 | 188 | 25 | 109 | 119 | 97 | 118 | 89 | 94 | 94 | 26 | 35 | 69 | |
Labelled Cell Count | 72 | 88 | 102 | 126 | 256 | 319 | 355 | 354 | 185 | 223 | 167 | 188 | 29 | 110 | 126 | 107 | 129 | 89 | 94 | 94 | 26 | 35 | 69 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.0 | 17.6 | 14.9 | 15.4 | 16.2 | 16.8 | 16.0 | 17.0 | 17.1 | 16.6 | 18.1 | 17.2 | – | 17.8 | 18.8 | 19.2 | 18.6 | 20.2 | – | – | 20.8 |
Std Deviation (1e-4) | 2.8 | 4.9 | 3.0 | 2.4 | 2.6 | 3.1 | 3.3 | 3.3 | 3.6 | 3.6 | 4.1 | 3.6 | – | 3.0 | 3.0 | 5.7 | 5.4 | 6.2 | – | – | 2.8 |
Intensity Change (Log2) | – | – | – | 0.05 | 0.12 | 0.17 | 0.1 | 0.19 | 0.2 | 0.16 | 0.28 | 0.21 | – | 0.26 | 0.33 | 0.36 | 0.32 | 0.44 | – | – | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.6 | 1.5 | 0.8 | 0 | 0.2 | -0.1 | 0.1 | 0.1 | -0.4 | – | -0.6 | 0.1 | -0.6 | -1.5 | -0.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.1102 | 11.5393 | 9.9824 | 11.8268 | 10.2275 | 10.7117 | 11.778 | 15.7616 | 12.0305 | 11.7806 | 13.7999 | 13.1523 | 6.9331 | 12.8811 | 9.4255 | 6.5115 | 11.2211 | 12.0738 |
Actin | 0.0867 | 0.0005 | 0.0894 | 0.0002 | 0.0001 | 0.0002 | 0.084 | 0.0006 | 0.0018 | 0.001 | 0.0001 | 0.0002 | 0.3078 | 0.0002 | 0.1051 | 0 | 0.0003 | 0.0001 |
Bud | 0.0018 | 0.0002 | 0.0016 | 0.0001 | 0.0001 | 0.0002 | 0.0021 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0029 | 0.0002 | 0.0028 | 0.0005 | 0.0003 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0006 | 0.0008 | 0.0022 | 0.0001 | 0.0009 | 0.0001 | 0.0005 | 0.0003 |
Bud Periphery | 0.0027 | 0 | 0.0013 | 0 | 0 | 0 | 0.0024 | 0 | 0.0004 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0095 | 0.001 | 0.0125 | 0 | 0 | 0.0001 | 0.0029 | 0.0002 | 0.0003 | 0.0001 | 0 | 0 | 0.0007 | 0.0001 | 0.0016 | 0 | 0 | 0 |
Cell Periphery | 0.0007 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6545 | 0.9529 | 0.7415 | 0.9671 | 0.646 | 0.9313 | 0.7092 | 0.9888 | 0.8156 | 0.8982 | 0.9874 | 0.9677 | 0.6606 | 0.9896 | 0.8196 | 0.6372 | 0.9866 | 0.9924 |
Cytoplasmic Foci | 0.039 | 0.0043 | 0.0328 | 0.0015 | 0.0003 | 0.003 | 0.0628 | 0.0004 | 0.0022 | 0.0034 | 0.0006 | 0.002 | 0.0033 | 0.0003 | 0.0241 | 0.0181 | 0.0016 | 0.0006 |
Eisosomes | 0.0006 | 0 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0305 | 0.0013 | 0.0084 | 0.0004 | 0.0004 | 0.0013 | 0.0036 | 0.0003 | 0.0683 | 0.0016 | 0.0004 | 0.001 | 0.0019 | 0.0002 | 0.0007 | 0.0068 | 0.0006 | 0.0004 |
Endosome | 0.0442 | 0.0034 | 0.0411 | 0.0004 | 0.0002 | 0.0273 | 0.0272 | 0.0001 | 0.0197 | 0.0008 | 0.0001 | 0.0006 | 0.0032 | 0.0001 | 0.0014 | 0.2964 | 0.0002 | 0.0001 |
Golgi | 0.0136 | 0.0002 | 0.0297 | 0 | 0 | 0.0006 | 0.0094 | 0 | 0.0029 | 0.0001 | 0 | 0 | 0.0067 | 0 | 0.0027 | 0.0158 | 0 | 0 |
Lipid Particles | 0.0131 | 0.0002 | 0.0046 | 0 | 0 | 0 | 0.0256 | 0 | 0.0009 | 0 | 0 | 0 | 0.0018 | 0 | 0.0006 | 0.0001 | 0 | 0 |
Mitochondria | 0.0157 | 0.0001 | 0.0037 | 0.0001 | 0.0001 | 0.0004 | 0.0059 | 0.0001 | 0.0027 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.001 | 0.0007 | 0 | 0 |
None | 0.0099 | 0.0191 | 0.0011 | 0.0012 | 0.0004 | 0.0008 | 0.0056 | 0.0004 | 0.0631 | 0.0022 | 0.0004 | 0.0046 | 0.0023 | 0.0004 | 0.001 | 0.0009 | 0.0014 | 0.0005 |
Nuclear Periphery | 0.022 | 0.0022 | 0.0051 | 0.0024 | 0.0084 | 0.0028 | 0.0132 | 0.0008 | 0.0082 | 0.0025 | 0.0009 | 0.0021 | 0.0012 | 0.0011 | 0.0011 | 0.0081 | 0.0008 | 0.0005 |
Nucleolus | 0.0009 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0193 | 0.0104 | 0.0105 | 0.0244 | 0.339 | 0.0206 | 0.0086 | 0.0078 | 0.0042 | 0.0884 | 0.0088 | 0.0196 | 0.0037 | 0.0074 | 0.0116 | 0.0059 | 0.0064 | 0.0048 |
Peroxisomes | 0.0114 | 0.0001 | 0.0087 | 0 | 0 | 0 | 0.0268 | 0 | 0 | 0 | 0 | 0 | 0.0012 | 0 | 0.0262 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0099 | 0.0017 | 0.0025 | 0.0014 | 0.0045 | 0.0007 | 0.0083 | 0.0002 | 0.0003 | 0.0009 | 0.0002 | 0.0009 | 0.0012 | 0.0001 | 0.0018 | 0.0003 | 0.0014 | 0.0001 |
Vacuole | 0.0069 | 0.0018 | 0.0013 | 0.0002 | 0.0001 | 0.0071 | 0.0007 | 0.0001 | 0.0067 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0061 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0041 | 0.0002 | 0.0005 | 0.0001 | 0 | 0.0028 | 0.0008 | 0 | 0.0016 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0032 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 96.7899 | 94.3901 | 53.6985 | 98.4987 | 96.9713 | 100.254 | 95.4377 | 107.3567 | 105.6341 | 111.8049 |
Translational Efficiency | 2.335 | 1.9719 | 2.6588 | 1.7655 | 2.0308 | 2.4405 | 2.0151 | 1.5326 | 1.841 | 1.8306 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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