Standard name
Human Ortholog
Description S-adenosylmethionine-dependent methyltransferase; required for wybutosine formation in phenylalanine-accepting tRNA; member of the seven beta-strand family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.99 0.98 0.98 0.91 0.78 0.76 0.68 0.69 0.55 0.61 1.0 1.0 1.0 0.99 0.97 0.94 0.91 0.96 0.91 0.92 0.89 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0 0.22 0.38 0.35 0.5 0.55 0.65 0.58 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 1 4 4
Bud 4 0 4 1 1 1 4 5 8 5 5 2 1 1 1 1 0 0 2 0 0 1 5 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 2 2 0 2 2 5 5 5 4 6 9 2 17 3 16 17 5 0 0 0 0 0 0
Cytoplasm 249 191 148 187 309 331 335 372 191 227 138 215 256 464 559 382 203 154 264 185 131 119 337 332
Endoplasmic Reticulum 1 1 0 0 1 0 7 5 1 3 3 2 1 1 0 25 4 4 4 0 3 0 5 6
Endosome 1 0 0 0 2 1 2 4 3 1 1 2 0 1 0 1 1 4 1 0 0 1 6 10
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 2 7
Mitochondria 6 0 5 13 9 81 162 171 140 180 164 203 0 1 2 6 2 3 2 1 2 0 4 10
Nucleus 4 2 0 0 1 0 2 1 2 0 1 2 2 0 1 5 4 1 1 0 0 0 0 0
Nuclear Periphery 2 1 0 0 0 0 1 0 2 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0
Nucleolus 2 0 1 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 3 4
Vac/Vac Membrane 4 0 0 2 1 0 11 10 7 4 5 6 0 1 0 3 5 2 7 2 1 0 2 13
Unique Cell Count 262 194 149 190 316 364 432 490 280 328 252 353 257 466 560 386 209 164 291 193 145 129 379 405
Labelled Cell Count 278 197 160 204 326 416 529 574 359 424 325 443 262 486 566 439 237 173 291 193 145 129 379 405


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 6.0 6.2 6.1 5.8 5.1 4.1 4.5 4.4 3.9 4.2 4.0 6.6 6.3 6.1 6.6 7.3 8.0 6.3 6.4 7.1
Std Deviation (1e-4) 0.7 0.9 1.4 1.2 1.5 1.2 0.7 0.9 1.3 0.9 1.1 0.7 1.2 1.1 1.0 1.4 2.0 2.1 1.3 1.3 1.8
Intensity Change (Log2) – – – -0.02 -0.1 -0.3 -0.61 -0.45 -0.5 -0.66 -0.57 -0.64 0.08 0.02 -0.03 0.08 0.24 0.37 0.02 0.05 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1.6 2.8 0
Cytoplasm -0.8 -1.2 -3.5 -6.1 -6.4 -7.5 -7.4 -9.5 -8.8 0.4 0.4 1.0 -0.4 -1.5 -2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.4 0 5.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4157 2.1924 1.2154 1.1299 0.7047 1.2872 2.0827 2.6067 2.5459 2.2478 1.9812 2.4492 3.3272 4.4756 3.8201 3.976 2.9075 3.7758
Actin 0.0046 0.0002 0.0035 0.0007 0.002 0.0005 0.0423 0.0143 0.023 0.0473 0.0018 0.0108 0.0246 0.0019 0.0012 0.005 0.0541 0.0067
Bud 0.0032 0.0003 0.0004 0.0061 0.0057 0.0003 0.0013 0.0028 0.001 0.0035 0.0011 0.0012 0.005 0.0113 0.0044 0.0005 0.0014 0.0017
Bud Neck 0.0003 0.0002 0.0005 0.0004 0.0006 0.0011 0.0146 0.0003 0.0005 0.0011 0.0024 0.0021 0.0022 0.0004 0.0002 0.0009 0.0003 0.0022
Bud Periphery 0.0018 0.0002 0.0004 0.0062 0.0017 0.0002 0.0018 0.0028 0.001 0.0045 0.0009 0.0033 0.004 0.0053 0.0018 0.0005 0.0038 0.0015
Bud Site 0.0037 0.0021 0.002 0.0018 0.0016 0.0002 0.0141 0.0124 0.0037 0.0068 0.0057 0.0007 0.0037 0.0213 0.0051 0.0018 0.0003 0.0004
Cell Periphery 0.0007 0.0001 0.0003 0.0006 0.0002 0.0001 0.0008 0.0003 0.0003 0.0005 0.0005 0.0002 0.0006 0.0004 0.0003 0.0002 0.0001 0.0001
Cytoplasm 0.5946 0.7924 0.6687 0.6918 0.5521 0.7315 0.531 0.7016 0.6278 0.5738 0.8125 0.6865 0.6256 0.7936 0.7606 0.7785 0.8198 0.8154
Cytoplasmic Foci 0.0126 0.0126 0.0165 0.017 0.0154 0.0108 0.0436 0.0146 0.021 0.0272 0.0107 0.0126 0.0222 0.0064 0.0089 0.008 0.0091 0.0087
Eisosomes 0.0004 0 0.0001 0.0001 0.0002 0.0001 0.0005 0.0001 0.0006 0.0004 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0038 0.0014 0.0034 0.0027 0.0024 0.0017 0.0062 0.0015 0.0027 0.0043 0.0039 0.003 0.0043 0.001 0.0023 0.003 0.0008 0.0009
Endosome 0.0057 0.0043 0.0146 0.0112 0.0073 0.0061 0.0295 0.0044 0.0141 0.0108 0.0083 0.0096 0.0165 0.0015 0.0029 0.0075 0.0019 0.0036
Golgi 0.0006 0.0004 0.0008 0.0003 0.0004 0.0002 0.0111 0.003 0.0056 0.0084 0.0008 0.0054 0.0098 0.0002 0.0003 0.0009 0.0016 0.0019
Lipid Particles 0.0007 0.0001 0.0006 0.0005 0.0006 0.0003 0.014 0.0002 0.0031 0.0084 0.0005 0.0033 0.0081 0.0001 0.0002 0.0003 0.0002 0.0012
Mitochondria 0.0006 0.0002 0.0005 0.001 0.0006 0.0004 0.0085 0.0103 0.0059 0.0041 0.0004 0.0048 0.0131 0.0016 0.0015 0.0006 0.0027 0.0005
None 0.3568 0.1788 0.278 0.2411 0.3937 0.2411 0.2366 0.2246 0.2694 0.2758 0.144 0.2408 0.2322 0.1493 0.2015 0.1856 0.101 0.1481
Nuclear Periphery 0.0012 0.0004 0.0015 0.0018 0.0012 0.0005 0.0024 0.0003 0.0009 0.0017 0.0006 0.0008 0.0019 0.0003 0.0025 0.0012 0.0002 0.0011
Nucleolus 0.0003 0.0001 0.0003 0.0009 0.0005 0.0001 0.0007 0.0001 0.0002 0.0015 0.0003 0.0001 0.001 0.0005 0.0007 0.0001 0 0.0002
Nucleus 0.0023 0.0021 0.0022 0.0042 0.0045 0.0022 0.0032 0.0009 0.001 0.0019 0.0016 0.0007 0.0016 0.0018 0.0021 0.0018 0.0007 0.0015
Peroxisomes 0.0004 0.0004 0.0006 0.0002 0.0003 0.0002 0.0232 0.0022 0.0146 0.0097 0.0004 0.0115 0.0158 0.0003 0.0002 0.0004 0.0011 0.0009
Punctate Nuclear 0.0018 0.0006 0.0017 0.0053 0.0069 0.0007 0.0065 0.0017 0.0016 0.005 0.0005 0.0006 0.0051 0.0013 0.0017 0.0008 0.0002 0.0025
Vacuole 0.0033 0.0027 0.003 0.0055 0.0016 0.0016 0.0075 0.0012 0.0016 0.0027 0.0027 0.0014 0.0022 0.0011 0.0012 0.002 0.0006 0.0006
Vacuole Periphery 0.0003 0.0002 0.0004 0.0007 0.0003 0.0002 0.0007 0.0002 0.0003 0.0006 0.0003 0.0003 0.0005 0.0001 0.0002 0.0003 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.7242 2.8405 7.9043 10.2771 4.6348 13.4971 8.0777 8.9653 10.3715 11.5298
Translational Efficiency 0.9327 2.99 1.318 0.5599 1.5068 0.641 1.0404 0.72 0.66 0.8885

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1090 1229 413 1486 1403 1707 1476 400 2493 2936 1889 1886

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 724.96 815.81 946.72 954.72 754.19 795.37 1000.76 960.38 741.41 803.93 988.95 955.92
Standard Deviation 79.55 113.60 127.81 143.90 101.52 120.95 118.93 222.64 93.69 118.36 122.98 163.81
Intensity Change Log 2 — 0.170332 0.385036 0.397176 — 0.076698 0.408096 0.348677 — 0.123349 0.396840 0.372651

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000088 0.000200 0.000260 0.000782 0.000081 0.000187 0.000164 0.000552 0.000084 0.000192 0.000185 0.000733
Bud Neck 0.003676 0.005843 0.000870 0.004393 0.001555 0.012544 0.001440 0.004770 0.002482 0.009739 0.001315 0.004473
Bud Site 0.004806 0.021652 0.003284 0.039466 0.002356 0.021093 0.006784 0.048582 0.003427 0.021327 0.006019 0.041400
Cell Periphery 0.000161 0.000065 0.000097 0.000073 0.000120 0.000074 0.000066 0.001213 0.000138 0.000070 0.000073 0.000315
Cytoplasm 0.694691* 0.677597* 0.693509* 0.732545* 0.752042* 0.601918* 0.763881* 0.558682* 0.726967* 0.633597* 0.748495* 0.695670*
Cytoplasmic Foci 0.149413 0.144397 0.024246 0.031723 0.107763 0.170899 0.018962 0.045075 0.125973 0.159805 0.020117 0.034555
Eisosomes 0.000098 0.000027 0.000020 0.000014 0.000040 0.000053 0.000016 0.000037 0.000065 0.000042 0.000017 0.000019
Endoplasmic Reticulum 0.000565 0.000601 0.001568 0.000364 0.000489 0.000587 0.001322 0.000367 0.000522 0.000593 0.001375 0.000365
Endosome 0.007827 0.019529 0.002187 0.003798 0.004118 0.028908 0.002197 0.012709 0.005740 0.024982 0.002195 0.005688
Golgi 0.001344 0.003703 0.000127 0.001985 0.000864 0.005772 0.000362 0.001761 0.001074 0.004906 0.000311 0.001937
Lipid Particles 0.002426 0.001473 0.000708 0.000604 0.001916 0.002696 0.000561 0.000941 0.002139 0.002184 0.000593 0.000676
Mitochondria 0.002422 0.001655 0.000120 0.001575 0.001465 0.004268 0.000292 0.002879 0.001883 0.003174 0.000254 0.001852
Mitotic Spindle 0.001204 0.003199 0.003622 0.031119 0.000100 0.003940 0.005113 0.028657 0.000583 0.003629 0.004787 0.030597
None 0.005148 0.002907 0.005681 0.003243 0.008445 0.001155 0.003966 0.009548 0.007003 0.001888 0.004341 0.004580
Nuclear Periphery 0.000135 0.000091 0.000490 0.000197 0.000133 0.000141 0.000471 0.000363 0.000134 0.000120 0.000476 0.000232
Nuclear Periphery Foci 0.000468 0.000933 0.004411 0.002465 0.000524 0.000683 0.002495 0.001162 0.000500 0.000788 0.002914 0.002189
Nucleolus 0.000607 0.000417 0.000127 0.000160 0.000480 0.000877 0.000115 0.000430 0.000536 0.000684 0.000118 0.000218
Nucleus 0.081675 0.020940 0.193057* 0.043481 0.091538 0.019181 0.103683 0.077422 0.087226 0.019917 0.123223 0.050679
Peroxisomes 0.001784 0.002612 0.000126 0.003006 0.000523 0.004055 0.000323 0.004022 0.001074 0.003451 0.000280 0.003221
Vacuole 0.040867 0.089808 0.065381 0.098595 0.024568 0.118181 0.087374 0.197013 0.031694 0.106304 0.082566 0.119469
Vacuole Periphery 0.000594 0.002352 0.000108 0.000411 0.000881 0.002787 0.000412 0.003816 0.000756 0.002605 0.000346 0.001133

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.42 -5.89 -4.44 -3.13 -0.39 -5.70 -2.70 -6.12 -4.60 -4.19 -6.11 -4.45 -6.01 -4.29 -3.30
Bud Neck -3.89 7.18 0.34 3.07 -3.43 -12.85 0.60 -3.86 7.65 -3.87 -13.11 4.36 -2.14 8.29 -4.12
Bud Site -8.09 0.42 -6.12 1.35 -7.18 -14.58 -4.03 -7.68 -4.21 -7.06 -15.50 -2.23 -10.44 -1.20 -9.41
Cell Periphery 12.51 3.82 13.41 1.77 6.73 7.94 9.65 -6.39 -6.79 -6.89 14.33 11.98 -3.51 -6.10 -5.92
Cytoplasm 1.86 5.13 9.00 6.26 0.91 17.46 9.78 15.83 6.13 10.46 14.39 9.54 18.04 5.47 8.38
Cytoplasmic Foci 0.96 26.03 27.37 23.44 -1.99 -11.64 26.08 16.26 26.17 -5.41 -8.10 38.53 32.22 37.62 -7.02
Eisosomes 17.52 15.88 20.94 5.92 4.89 -2.74 10.36 1.55 3.47 -5.21 7.41 19.96 20.63 7.81 0.76
Endoplasmic Reticulum -0.19 -6.85 1.56 2.51 8.50 -0.75 -3.01 1.85 1.57 3.47 -0.67 -4.86 2.46 2.69 5.94
Endosome -6.76 5.79 6.03 11.47 0.63 -15.37 6.19 -1.50 9.36 -2.81 -16.16 8.37 4.09 18.24 -2.80
Golgi -3.71 4.62 2.03 4.93 -1.70 -6.96 5.01 0.05 6.23 -1.68 -7.83 6.20 1.66 8.15 -1.95
Lipid Particles 2.74 8.49 8.74 1.42 -0.23 -1.25 7.51 4.80 3.12 -1.34 -0.01 11.92 9.25 3.38 -3.20
Mitochondria 0.61 1.84 1.53 1.92 -1.18 -2.86 3.98 0.00 2.61 -2.35 -1.54 2.96 1.92 3.90 -1.95
Mitotic Spindle -1.72 -1.37 -8.01 -7.11 -6.36 -4.21 -5.38 -5.01 -4.25 -3.92 -4.23 -5.02 -9.67 -8.25 -7.44
None 2.72 0.52 3.56 -0.26 2.58 7.05 4.77 1.93 -3.65 -1.64 7.47 4.91 5.05 -3.84 0.73
Nuclear Periphery 2.78 -12.05 -15.14 -16.77 2.90 -0.25 -21.01 -9.34 -8.35 2.69 0.89 -24.47 -18.03 -16.50 4.61
Nuclear Periphery Foci -3.59 -10.03 -5.41 -3.67 5.27 -1.20 -11.51 -5.79 -4.08 4.54 -3.13 -15.25 -6.09 -4.72 3.57
Nucleolus 1.22 2.99 2.26 3.17 -3.91 -2.03 8.22 1.65 2.61 -3.78 -1.07 5.66 3.47 3.59 -5.88
Nucleus 21.35 -12.82 5.54 -16.40 14.76 22.04 -6.12 4.18 -8.54 8.56 30.16 -12.89 8.12 -19.15 18.63
Peroxisomes -1.35 3.16 0.42 2.49 -4.02 -9.00 1.83 -1.46 1.78 -1.69 -6.94 3.34 -1.34 4.15 -3.85
Vacuole -12.56 -8.16 -29.36 -19.36 -12.62 -25.56 -24.95 -17.40 -11.06 -8.03 -27.56 -25.35 -35.20 -21.36 -12.82
Vacuole Periphery -5.15 5.18 4.49 6.66 -1.23 -5.20 4.76 -0.82 1.15 -1.54 -7.20 5.52 1.36 6.74 -1.29
Standard name
Human Ortholog
Description S-adenosylmethionine-dependent methyltransferase; required for wybutosine formation in phenylalanine-accepting tRNA; member of the seven beta-strand family
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Trm12

Trm12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trm12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available