Standard name
Human Ortholog
Description Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.46 0.32 0.27 0.21 0.23 0.27 0.31 0.28 0.24 0.25 0.21 0.19 0.46 0.44 0.37 0.2 0.17 0.2 0.05 0.09 0.07 0.13 0.11 0.07
Bud 0.08 0.08 0.06 0.07 0.09 0.08 0.09 0.11 0.11 0.14 0.13 0.12 0.09 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.63 0.64 0.63 0.69 0.48 0.54 0.46 0.27 0.39 0.33 0.23 0.34 0.75 0.74 0.82 0.69 0.62 0.53 0.72 0.62 0.6 0.65 0.8 0.81
Cytoplasm 0.13 0.3 0.3 0.35 0.44 0.39 0.4 0.46 0.46 0.48 0.47 0.52 0.18 0.17 0.11 0.17 0.2 0.35 0 0.07 0.05 0 0 0
Endoplasmic Reticulum 0.09 0.05 0 0 0 0.06 0.05 0.06 0 0 0 0.05 0.05 0 0.06 0.06 0 0.06 0.15 0.11 0.11 0.05 0 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.13 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0.1 0.13 0.09 0.26 0.25 0.27 0.15 0 0.05 0.05 0.06 0.07 0.08 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 90 73 75 64 88 105 182 122 89 107 86 81 153 206 175 25 16 16 12 25 24 24 46 36
Bud 15 19 16 22 37 30 50 47 40 58 52 53 30 19 12 5 0 4 1 1 2 1 0 1
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 4 14 24 11 8 8
Bud Site 2 3 1 0 0 0 2 0 0 2 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 122 148 177 211 188 206 265 116 143 143 92 144 248 345 385 86 60 42 171 172 220 119 331 406
Cytoplasm 26 68 86 108 171 150 234 201 170 204 191 224 60 80 50 21 19 28 5 19 17 3 1 1
Endoplasmic Reticulum 18 11 11 4 15 23 31 28 16 11 14 20 18 12 29 8 2 5 35 29 39 9 9 29
Endosome 0 0 0 0 0 0 0 0 0 0 1 0 0 3 0 0 1 2 0 0 1 0 0 0
Golgi 0 0 1 0 0 0 0 2 0 0 0 0 0 2 0 6 3 10 2 1 2 1 2 4
Mitochondria 14 3 0 2 17 37 75 37 97 108 109 63 7 25 23 7 7 6 0 7 22 6 8 8
Nucleus 0 0 0 1 2 0 3 5 1 2 3 0 3 0 0 2 2 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 8 0 1 2 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 2 59 6 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 5 3 2 1 6 7 2 7 6 7 0 0 1 3 5 0 0 0 1 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 14 1 2 7 1 0 0 0 0 0 5 2 4 6 4 1 3
Unique Cell Count 194 230 283 306 390 382 580 433 371 429 403 427 330 465 469 124 96 79 239 279 367 183 414 505
Labelled Cell Count 288 325 372 415 520 552 848 588 559 645 564 593 522 751 681 163 116 118 239 279 367 183 414 505


Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 8.6 8.3 7.9 8.0 7.1 7.5 8.0 6.6 6.4 6.2 6.9 8.9 9.5 9.6 13.1 12.8 12.4 8.2 8.6 9.8
Std Deviation (1e-4) 1.2 1.4 1.0 0.9 1.4 1.0 1.0 1.0 1.1 1.0 1.0 0.8 1.1 1.3 1.2 2.7 2.2 3.0 1.0 1.4 1.6
Intensity Change (Log2) – – – -0.08 -0.06 -0.24 -0.16 -0.07 -0.33 -0.38 -0.43 -0.28 0.09 0.19 0.2 0.65 0.61 0.57 -0.02 0.05 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.6 -1.2 0.3 1.5 0.5 -0.7 -0.5 -1.6 -2.4 5.1 4.9 3.0 -1.4 -1.9 -1.1
Bud 0.8 1.8 1.1 1.5 2.4 2.3 3.4 3.1 3.0 1.6 -1.0 -2.2 -0.7 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.6 -3.7 -2.2 -4.7 -9.5 -6.1 -7.7 -10.5 -7.6 3.4 3.4 6.0 1.3 0 -1.5
Cytoplasm 1.3 3.5 2.4 2.8 4.3 4.0 4.6 4.5 5.8 -3.5 -4.2 -6.8 -2.8 -2.0 0.9
Endoplasmic Reticulum -2.0 0 1.2 0.9 1.5 0.3 -1.0 -0.3 0.5 0.9 -1.0 1.4 1.1 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.6 5.4 6.3 5.0 9.3 9.2 9.5 6.8 0 4.0 3.8 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 6.2 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.1 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0083 4.4087 4.1478 4.0074 2.982 4.2759 3.7693 4.7344 4.564 4.5442 3.5631 4.5792 5.0664 6.0837 5.3747 5.7626 5.1961 5.6741
Actin 0.0304 0.0248 0.0315 0.0248 0.0963 0.0358 0.0619 0.0277 0.0234 0.0038 0.0196 0.0175 0.033 0.0146 0.035 0.0124 0.012 0.0136
Bud 0.0008 0.001 0.0017 0.0028 0.0011 0.0009 0.0026 0.0005 0.0018 0.0014 0.0009 0.0007 0.0008 0.0076 0.0025 0.0026 0.0008 0.0006
Bud Neck 0.005 0.0053 0.0165 0.0082 0.0288 0.0401 0.0083 0.0072 0.0144 0.0103 0.0146 0.0222 0.0061 0.0073 0.0178 0.0179 0.0202 0.0517
Bud Periphery 0.0084 0.0062 0.0133 0.0647 0.0146 0.0129 0.0126 0.0027 0.0156 0.0352 0.013 0.0137 0.0061 0.0045 0.0191 0.0185 0.0126 0.0115
Bud Site 0.0177 0.0854 0.0501 0.0036 0.004 0.0036 0.0267 0.1201 0.0665 0.0016 0.0042 0.002 0.0144 0.094 0.0358 0.0071 0.0035 0.0018
Cell Periphery 0.5562 0.4369 0.5855 0.6217 0.5606 0.5856 0.5683 0.5669 0.6534 0.7408 0.5747 0.7574 0.6336 0.5587 0.6023 0.7148 0.597 0.671
Cytoplasm 0.2032 0.3244 0.2331 0.2079 0.1928 0.2307 0.1276 0.2368 0.143 0.1213 0.1744 0.1052 0.1737 0.2626 0.179 0.0682 0.2096 0.169
Cytoplasmic Foci 0.0024 0.0054 0.004 0.0006 0.0044 0.0023 0.0145 0.0009 0.0022 0.0013 0.0124 0.001 0.008 0.0007 0.0136 0.0055 0.0082 0.001
Eisosomes 0.0534 0.0221 0.015 0.0091 0.0199 0.0228 0.0445 0.0102 0.023 0.0155 0.0342 0.0197 0.0162 0.0093 0.007 0.0466 0.002 0.0048
Endoplasmic Reticulum 0.0687 0.0513 0.0278 0.0451 0.0118 0.0392 0.0347 0.0135 0.0302 0.048 0.0179 0.0453 0.0504 0.0294 0.0343 0.0666 0.0432 0.0549
Endosome 0.0055 0.0042 0.0013 0.0003 0.011 0.0016 0.0121 0.0003 0.0028 0.0058 0.0121 0.0031 0.0041 0.0002 0.0128 0.0038 0.0047 0.0027
Golgi 0.0072 0.009 0.0049 0.0019 0.01 0.003 0.0151 0.0019 0.0049 0.0018 0.0215 0.0041 0.0047 0.0011 0.0063 0.0083 0.0045 0.0032
Lipid Particles 0.0055 0.006 0.0037 0.0016 0.0214 0.0031 0.0219 0.0034 0.0027 0.0025 0.0257 0.0029 0.0093 0.0035 0.0096 0.0026 0.0468 0.0064
Mitochondria 0.0053 0.0006 0.0007 0.0006 0.0112 0.0004 0.0157 0.0001 0.007 0.0005 0.0567 0.0005 0.0077 0.0003 0.0022 0.0142 0.0014 0.0004
None 0.0119 0.0123 0.0032 0.0009 0.0056 0.0074 0.0052 0.0038 0.001 0.004 0.0004 0.0005 0.0032 0.0006 0.001 0.0005 0.0012 0.0004
Nuclear Periphery 0.0052 0.001 0.0008 0.0008 0.0004 0.0055 0.0049 0.001 0.0011 0.0019 0.0005 0.0008 0.0097 0.0016 0.0018 0.0018 0.0033 0.0022
Nucleolus 0.0003 0.0001 0.0001 0.0001 0.0002 0.0002 0.0004 0.0001 0.0001 0.0001 0.0005 0.0001 0.0005 0.0001 0.0007 0.0004 0.0002 0.0001
Nucleus 0.0063 0.0003 0.0004 0.0003 0.0002 0.001 0.0026 0.0003 0.0004 0.0005 0.0002 0.0002 0.0009 0.0004 0.0007 0.0007 0.0002 0.0003
Peroxisomes 0.0003 0.0001 0.003 0 0.0031 0.0001 0.0128 0 0.003 0 0.0126 0.0002 0.0034 0 0.0114 0.0019 0.0183 0.0004
Punctate Nuclear 0.0005 0.0001 0.0002 0 0.0001 0.0005 0.0016 0.0001 0.0001 0.0001 0.0003 0.0001 0.009 0.0001 0.0006 0.0003 0.0078 0.0009
Vacuole 0.0047 0.0033 0.003 0.0049 0.0019 0.0032 0.0051 0.0023 0.0031 0.0034 0.0026 0.0025 0.0043 0.0033 0.0062 0.0048 0.002 0.0032
Vacuole Periphery 0.0011 0.0001 0.0001 0.0002 0.0005 0.0002 0.0008 0 0.0002 0.0003 0.0007 0.0001 0.0009 0 0.0004 0.0004 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.7797 19.4523 15.4907 22.1483 21.3319 18.0351 25.1849 27.3589 23.154 30.7844
Translational Efficiency 1.107 1.0935 1.0651 0.6096 0.7243 1.4151 0.9494 0.8299 0.974 0.923

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
Localization
Cell Percentages cell periphery (79%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-2

Ppz1

Ppz1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ppz1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available