Standard name
Human Ortholog
Description Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 – – 0 0 0 0 – 0 0 0 0 0 0 0.07 0.06 0.05 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.89 – – 0.91 0.9 0.79 0.83 – 0.73 0.7 0.67 0.68 0.88 0.85 0.73 0.78 0.81 0.75 0.78 0.75 0.66 0.87 0.81 0.83
Nuclear Periphery 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.62 – – 0.26 0.17 0.38 0.3 – 0.43 0.49 0.73 0.55 0.23 0.27 0.33 0.22 0.21 0.27 0.16 0.2 0.32 0.1 0.12 0.08
Peroxisomes 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud Neck 0 – – 0 0 0 2 – 1 3 1 3 0 0 0 0 0 1 0 0 0 0 0 0
Bud Site 0 – – 0 0 0 3 – 2 5 1 4 0 1 2 0 0 1 – – – – – –
Cell Periphery 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 – – 4 7 3 2 – 7 4 3 5 3 9 16 19 19 15 0 0 0 0 1 1
Endoplasmic Reticulum 0 – – 0 0 1 0 – 0 0 0 0 0 1 0 2 1 3 0 0 0 0 0 0
Endosome 0 – – 0 2 0 2 – 1 2 0 0 1 1 9 3 5 4 0 0 0 0 0 1
Golgi 0 – – 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0
Mitochondria 3 – – 0 0 1 3 – 1 5 5 1 0 0 0 5 3 11 0 0 0 0 0 1
Nucleus 107 – – 157 224 143 298 – 136 185 77 157 70 185 159 257 310 215 93 40 11 32 107 132
Nuclear Periphery 0 – – 0 0 0 1 – 1 2 0 0 0 0 0 1 2 3 0 0 0 0 0 0
Nucleolus 74 – – 45 41 69 109 – 80 131 84 127 18 59 72 71 80 76 19 10 5 3 15 13
Peroxisomes 0 – – 0 0 0 1 – 0 0 0 0 0 1 2 3 1 2 0 0 0 0 0 0
SpindlePole 0 – – 0 0 0 2 – 0 2 0 1 1 1 9 6 4 4 0 0 0 0 0 0
Vac/Vac Membrane 0 – – 2 1 2 0 – 1 3 0 2 0 1 2 3 7 9 5 0 0 0 4 6
Unique Cell Count 120 172 248 181 359 187 266 115 230 80 217 217 328 384 285 120 54 18 37 134 161
Labelled Cell Count 185 208 275 219 423 230 342 171 300 93 259 271 371 433 344 120 54 18 37 134 161


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 – – 12.6 13.7 11.4 11.4 – 10.0 10.5 8.3 9.7 14.5 14.3 13.2 19.3 19.9 17.6 – 12.0 13.1
Std Deviation (1e-4) 2.9 – – 2.8 3.0 2.8 2.7 – 2.8 2.7 2.4 2.6 3.4 3.4 3.9 6.7 8.0 7.1 – 2.6 2.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.2123 9.7434 8.9116 8.7208 8.2968 8.8415 8.9427 9.043 8.9405 8.8632 7.6687 8.4308 8.1105 7.1262 6.9034 6.7202 6.5119 6.0146
Actin 0.0088 0 0.001 0 0.0001 0.0005 0.0273 0 0.0006 0.0037 0.0057 0.0052 0.0628 0 0.0212 0 0 0.0027
Bud 0.0007 0 0.0001 0 0.0005 0.0001 0.0006 0 0.0001 0.0001 0.0011 0.0001 0.0002 0 0.0002 0 0 0.0001
Bud Neck 0.0008 0 0.0001 0 0.0005 0.0003 0.0006 0 0.0002 0.0001 0.0003 0.0003 0.0007 0 0.0001 0 0.0001 0.0005
Bud Periphery 0.0013 0 0.0004 0 0.0015 0.0001 0.0008 0 0.0001 0.0001 0.0039 0.0002 0.0003 0 0.0002 0 0 0.0002
Bud Site 0.0016 0 0.0002 0 0.0008 0.0001 0.0025 0 0.0003 0.0001 0.0017 0.0002 0.0007 0 0.0013 0 0 0.0003
Cell Periphery 0.0006 0 0.0004 0 0.0006 0 0.0004 0 0 0 0.0003 0 0.0001 0 0.0001 0 0 0.0001
Cytoplasm 0.0083 0.0001 0.0001 0 0.0003 0.0001 0.0029 0 0.0008 0.0022 0 0.0001 0.0021 0 0.0004 0 0 0.0003
Cytoplasmic Foci 0.008 0 0.0007 0 0.0057 0.0006 0.0172 0 0.0051 0.0025 0.0009 0.0011 0.0067 0 0.0018 0 0 0.0064
Eisosomes 0.0001 0 0 0 0 0 0.0007 0 0 0.0001 0.0002 0 0.0004 0 0.0002 0 0 0
Endoplasmic Reticulum 0.006 0 0 0 0.0001 0.0001 0.0039 0 0.0004 0.0023 0.0001 0.0001 0.002 0 0.0004 0 0 0.0001
Endosome 0.0094 0 0.0005 0 0.0016 0.0008 0.0095 0 0.0068 0.0145 0.0017 0.0016 0.01 0 0.0022 0 0 0.002
Golgi 0.003 0 0.0004 0 0.0011 0.0008 0.0046 0 0.0013 0.001 0.0024 0.0021 0.0072 0 0.0016 0 0 0.0026
Lipid Particles 0.0113 0 0.0031 0 0.0132 0.0019 0.0076 0 0.0026 0.0021 0.0053 0.0006 0.0057 0 0.0012 0 0 0.0061
Mitochondria 0.0105 0.0001 0.0016 0 0.0022 0.0066 0.0071 0.0002 0.0009 0.0029 0.0379 0.0091 0.0019 0.0001 0.0059 0.0001 0.0002 0.0019
None 0.0035 0 0.0001 0 0.0002 0.0002 0.0103 0 0.0009 0.0023 0.0002 0.0002 0.0025 0 0.0005 0 0 0.0002
Nuclear Periphery 0.0143 0.0019 0.0008 0.0001 0.0016 0.0009 0.011 0.0001 0.0036 0.0538 0.0006 0.0002 0.0046 0.0002 0.0063 0 0.0001 0.0006
Nucleolus 0.1078 0.0352 0.0453 0.0362 0.3859 0.235 0.1063 0.0551 0.0525 0.0369 0.4348 0.2252 0.0909 0.0587 0.063 0.0329 0.1662 0.182
Nucleus 0.7825 0.9616 0.9288 0.9635 0.5623 0.7439 0.7545 0.9443 0.9202 0.8545 0.4966 0.7515 0.7864 0.9408 0.8882 0.9669 0.833 0.7843
Peroxisomes 0.0013 0 0.0019 0 0.0006 0.0003 0.0149 0 0.0008 0.0004 0.0036 0.0017 0.0022 0 0.0034 0 0 0.001
Punctate Nuclear 0.0153 0.0006 0.011 0 0.0134 0.0072 0.015 0 0.0016 0.0173 0.0007 0.0002 0.0114 0.0001 0.0011 0 0.0004 0.0077
Vacuole 0.0026 0.0002 0.0021 0 0.0046 0.0002 0.0012 0.0001 0.0006 0.0006 0.0008 0.0001 0.0005 0 0.0002 0 0 0.0005
Vacuole Periphery 0.0025 0.0003 0.0013 0 0.0032 0.0002 0.0013 0.0001 0.0004 0.0026 0.001 0.0001 0.0006 0 0.0004 0 0 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.7579 37.8506 23.0563 30.7727 31.9553 24.6328 39.9844 36.0466 34.3116 36.958
Translational Efficiency 1.1414 1.0543 1.3401 1.0953 1.1234 1.4864 1.0075 0.9691 1.0883 0.9887

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1526 947 662 1522 1406 1345 1409 1156 2932 2292 2071 2678

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 775.41 1089.51 1427.69 1380.38 976.02 1087.30 1333.40 1350.49 871.61 1088.21 1363.54 1367.48
Standard Deviation 147.60 205.38 300.35 276.39 208.63 208.25 244.95 280.56 205.56 207.07 267.56 278.59
Intensity Change Log 2 — 0.490648 0.880652 0.832034 — 0.155767 0.450127 0.468500 — 0.313682 0.656705 0.640827

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000565 0.000585 0.000715 0.000831 0.000466 0.000585 0.000680 0.000932 0.000517 0.000585 0.000691 0.000874
Bud Neck 0.004955 0.005070 0.007224 0.015755 0.002915 0.005292 0.009020 0.022453 0.003977 0.005200 0.008446 0.018646
Bud Site 0.001512 0.000448 0.000790 0.001606 0.000861 0.000913 0.000779 0.003209 0.001200 0.000721 0.000782 0.002298
Cell Periphery 0.000198 0.000020 0.000025 0.000074 0.000062 0.000044 0.000028 0.000167 0.000133 0.000034 0.000027 0.000114
Cytoplasm 0.003464 0.000086 0.000048 0.000327 0.000810 0.000457 0.000061 0.000753 0.002191 0.000304 0.000057 0.000511
Cytoplasmic Foci 0.000608 0.000806 0.000174 0.000484 0.000469 0.000270 0.000071 0.001546 0.000541 0.000492 0.000104 0.000942
Eisosomes 0.000061 0.000100 0.000108 0.000084 0.000096 0.000117 0.000111 0.000072 0.000077 0.000110 0.000110 0.000079
Endoplasmic Reticulum 0.001734 0.000803 0.000330 0.000511 0.000792 0.001461 0.000423 0.000753 0.001282 0.001189 0.000393 0.000616
Endosome 0.000292 0.000064 0.000012 0.000533 0.000102 0.000322 0.000016 0.001785 0.000201 0.000215 0.000014 0.001074
Golgi 0.000366 0.000425 0.000073 0.001028 0.000157 0.000145 0.000060 0.001241 0.000266 0.000260 0.000064 0.001120
Lipid Particles 0.000061 0.000114 0.000007 0.000063 0.000011 0.000227 0.000006 0.000119 0.000037 0.000180 0.000006 0.000087
Mitochondria 0.000843 0.003559 0.001426 0.002983 0.000671 0.001810 0.001393 0.004395 0.000761 0.002533 0.001404 0.003593
Mitotic Spindle 0.001662 0.002233 0.000104 0.005311 0.000518 0.002679 0.000479 0.008728 0.001113 0.002495 0.000359 0.006786
None 0.011330 0.000574 0.000701 0.001074 0.003333 0.000671 0.001193 0.001934 0.007496 0.000631 0.001036 0.001445
Nuclear Periphery 0.000139 0.000039 0.000035 0.001175 0.000025 0.000288 0.000052 0.000819 0.000085 0.000185 0.000047 0.001021
Nuclear Periphery Foci 0.000821 0.000146 0.000007 0.000394 0.000109 0.000802 0.000013 0.001023 0.000480 0.000531 0.000011 0.000665
Nucleolus 0.135620 0.135738 0.094288 0.084169 0.126626 0.128710 0.091328 0.087451 0.131307 0.131614 0.092274 0.085586
Nucleus 0.833656* 0.845356* 0.884787* 0.878129* 0.858279* 0.851304* 0.890022* 0.855351* 0.845464* 0.848846* 0.888349* 0.868296*
Peroxisomes 0.001216 0.003354 0.008776 0.003064 0.003141 0.003015 0.003917 0.002932 0.002139 0.003155 0.005470 0.003007
Vacuole 0.000448 0.000195 0.000180 0.001134 0.000327 0.000471 0.000153 0.002275 0.000390 0.000357 0.000162 0.001627
Vacuole Periphery 0.000448 0.000284 0.000189 0.001272 0.000230 0.000416 0.000195 0.002062 0.000344 0.000362 0.000193 0.001613

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.56 -5.04 -8.45 -7.13 -3.96 -3.10 -9.52 -13.58 -8.64 -8.05 -2.48 -9.46 -15.40 -10.75 -8.66
Bud Neck -0.47 -5.42 -11.81 -11.46 -9.11 -4.66 -12.19 -13.88 -11.85 -9.38 -3.59 -12.03 -18.30 -16.19 -12.29
Bud Site 3.23 2.04 -1.05 -5.19 -3.88 -0.21 0.08 -4.95 -5.23 -6.28 1.58 1.51 -4.39 -6.59 -7.35
Cell Periphery 5.47 5.25 3.30 -4.49 -3.94 0.67 1.43 -3.05 -4.20 -4.99 4.64 5.15 0.42 -5.46 -6.28
Cytoplasm 3.66 3.71 3.37 -2.25 -3.08 0.62 1.71 -0.01 -1.10 -4.33 3.46 4.07 3.11 -1.34 -5.28
Cytoplasmic Foci -0.31 1.52 0.44 0.51 -1.86 0.65 1.37 -1.65 -2.42 -2.95 0.09 2.15 -0.99 -0.86 -3.61
Eisosomes -6.00 -8.62 -4.74 2.33 4.33 -3.32 -3.13 3.90 7.45 8.52 -7.18 -9.13 -0.77 6.72 8.68
Endoplasmic Reticulum 7.68 12.47 10.37 4.33 -7.03 -1.47 7.70 -0.98 1.28 -5.72 0.32 13.80 8.40 1.83 -7.46
Endosome 3.10 4.01 -0.98 -2.28 -2.57 -1.73 1.82 -2.56 -2.09 -2.74 -0.17 4.35 -2.77 -2.65 -3.57
Golgi -0.13 1.33 -0.95 -0.81 -1.57 0.16 1.34 -2.07 -2.12 -2.33 0.06 1.68 -1.88 -1.90 -2.55
Lipid Particles -0.79 2.48 -0.16 0.68 -2.16 -2.14 1.71 -2.37 0.89 -2.45 -2.18 2.65 -1.95 1.25 -3.26
Mitochondria -1.90 -7.87 -9.07 0.24 -6.51 -1.67 -7.69 -5.88 -2.82 -4.70 -2.48 -10.62 -9.49 -1.55 -7.27
Mitotic Spindle -0.41 2.71 -3.01 -2.12 -4.57 -1.88 -0.07 -4.93 -3.62 -4.94 -1.54 1.78 -5.77 -4.09 -6.56
None 6.55 6.46 6.20 -4.50 -3.05 3.26 2.49 1.57 -5.05 -2.09 7.27 6.71 6.32 -6.42 -1.94
Nuclear Periphery 5.40 5.35 -2.40 -2.66 -2.65 -1.36 -5.15 -3.54 -1.79 -3.40 -0.88 3.42 -3.62 -2.92 -3.76
Nuclear Periphery Foci 2.59 3.38 1.90 -1.34 -3.45 -1.94 3.47 -1.53 -0.05 -1.73 -0.10 3.69 -0.20 -0.08 -2.59
Nucleolus 0.05 6.75 8.49 7.35 1.06 -0.32 6.29 5.69 5.86 -0.12 0.01 9.41 10.11 9.41 0.61
Nucleus -1.75 -7.36 -5.25 -3.10 2.01 1.13 -5.07 2.15 1.18 6.09 -0.81 -9.24 -2.37 -1.53 5.76
Peroxisomes -7.30 -9.44 -5.67 0.11 6.38 0.43 -2.35 -0.40 -0.88 1.93 -4.79 -9.99 -4.30 -0.52 5.84
Vacuole 1.86 1.92 -1.99 -2.80 -2.82 -1.13 1.51 -3.63 -3.38 -3.94 0.29 2.47 -4.07 -4.23 -4.84
Vacuole Periphery 1.57 2.52 -2.04 -2.59 -2.86 -2.12 0.85 -3.32 -2.89 -3.40 -0.25 2.64 -3.83 -3.79 -4.43
Standard name
Human Ortholog
Description Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Rsc9

Rsc9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsc9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available