Standard name
Human Ortholog
Description Copper-sensing transcription factor; involved in regulation of genes required for high affinity copper transport; required for regulation of yeast copper genes in response to DNA-damaging agents; undergoes changes in redox state in response to changing levels of copper or MMS
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.11 0.1 0.12 0.18 0.12 0.15 0.14 0.17 0.11 0.08 0.13 0.19 0.28 0.51 0.29 0.36 0.34 0.07 0.05 0 0.05 0.08 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.1 0.09 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.71 0.26 0.14 0.21 0.4 0.73 0.76 0.71 0.68 0.82 0.84 0.75 0 0 0 0.1 0.05 0.1 0 0 0 0 0 0
Nucleus 0.57 0.7 0.7 0.71 0.45 0.42 0.38 0.4 0.39 0.41 0.38 0.38 0.74 0.68 0.39 0.38 0.34 0.37 0.64 0.68 0.64 0.59 0.48 0.31
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0.07 0.09
Vac/Vac Membrane 0 0.11 0.09 0.09 0.13 0.08 0.07 0.06 0.07 0.05 0.07 0.05 0 0.08 0.13 0.16 0.2 0.19 0.19 0.15 0.21 0.21 0.24 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud 0 0 0 2 4 2 16 6 1 7 13 10 0 0 0 1 0 0 1 2 1 5 6 7
Bud Neck 0 1 0 1 0 0 0 1 0 0 1 0 3 2 3 0 0 0 1 0 0 1 3 4
Bud Site 0 0 0 1 2 4 8 18 3 10 9 15 0 0 0 0 2 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1 1 4 0 0 0 0 0 0
Cytoplasm 37 24 25 21 40 35 56 43 52 41 29 47 47 103 181 111 110 91 22 14 10 17 26 40
Endoplasmic Reticulum 0 1 1 0 0 0 0 0 0 1 0 0 2 1 2 24 10 21 0 0 3 0 0 3
Endosome 0 0 7 3 7 1 0 1 0 0 0 0 2 0 5 49 30 23 3 4 3 4 5 13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 1 0 0 1
Mitochondria 219 56 35 37 86 207 274 214 202 300 296 272 2 0 2 38 15 27 6 7 8 8 10 15
Nucleus 177 151 171 127 98 121 139 119 116 149 134 137 188 248 140 145 105 98 207 176 197 189 156 107
Nuclear Periphery 0 0 0 2 0 2 0 1 4 2 3 4 0 0 0 0 0 1 0 0 2 3 0 1
Nucleolus 0 0 0 1 0 1 1 1 2 1 1 2 0 0 0 6 3 5 1 3 3 8 9 4
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 1 0 0 0 0 0 0
SpindlePole 2 0 2 1 0 2 1 4 2 2 0 2 4 2 2 14 20 18 7 7 4 11 22 32
Vac/Vac Membrane 3 23 23 17 29 23 25 19 20 19 26 17 10 29 47 62 61 51 60 39 63 66 79 102
Unique Cell Count 308 215 243 179 217 285 362 300 299 365 351 365 254 365 356 379 305 266 324 261 307 322 330 347
Labelled Cell Count 438 256 264 213 266 398 520 427 402 533 512 507 260 386 382 453 358 341 324 261 307 322 330 347


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 4.5 4.4 4.2 4.2 3.7 3.5 3.6 3.6 3.2 3.0 3.0 5.8 5.9 5.7 6.1 5.7 5.3 6.2 6.4 6.8
Std Deviation (1e-4) 0.4 0.8 1.1 1.6 1.7 2.0 2.0 1.7 1.7 1.5 0.9 1.0 1.1 1.0 1.0 1.7 1.8 1.5 1.2 1.3 1.6
Intensity Change (Log2) – – – -0.06 -0.08 -0.27 -0.34 -0.31 -0.3 -0.45 -0.58 -0.54 0.39 0.4 0.36 0.47 0.37 0.26 0.48 0.54 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.4 3.4 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9533 1.2933 0.9142 0.943 0.6042 1.0007 1.6282 1.2195 1.2076 1.2131 1.3397 1.2134 0.6131 0.8859 0.6095 0.4235 0.4955 0.5653
Actin 0.0058 0.0002 0 0.006 0.0001 0.0005 0.0683 0.0003 0.0142 0.0048 0.0155 0.0072 0.017 0.0001 0.0054 0.0002 0.0001 0.0002
Bud 0.0001 0 0 0.0001 0 0.0001 0.0008 0.0014 0.0005 0.0002 0.0009 0.0001 0.0004 0.0005 0 0 0 0
Bud Neck 0.003 0.0002 0.0001 0.0004 0.001 0.0028 0.017 0.0004 0.0093 0.0055 0.0029 0.0034 0.0148 0.0004 0.0003 0.0008 0.0007 0.0013
Bud Periphery 0.0004 0.0001 0 0.0001 0 0.0002 0.0013 0.0002 0.0007 0.0004 0.0022 0.0003 0.001 0.0003 0 0 0 0
Bud Site 0.0005 0.0002 0 0.0004 0.0001 0.0004 0.0048 0.0008 0.002 0.0015 0.0014 0.0002 0.0013 0.0009 0.0003 0.0001 0.0001 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.001 0.0001 0.0003 0.0001 0.0002 0 0.0002 0 0 0 0 0
Cytoplasm 0.0142 0.0273 0.0093 0.0137 0.0059 0.0054 0.0303 0.0214 0.0155 0.0016 0.0173 0.0075 0.006 0.0188 0.0139 0.0291 0.0271 0.0056
Cytoplasmic Foci 0.0007 0.0007 0.0001 0.0041 0.0001 0.0006 0.0158 0.0009 0.0031 0.0026 0.0053 0.001 0.0024 0.0033 0.0005 0.0001 0.001 0.0003
Eisosomes 0.0001 0 0 0.0001 0 0 0.001 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0026 0.0008 0.0002 0.001 0.0002 0.0003 0.011 0.0006 0.0013 0.0003 0.0037 0.0003 0.0019 0.0008 0.0009 0.0003 0.0011 0.0002
Endosome 0.0051 0.0006 0.0002 0.0064 0.0004 0.0007 0.0366 0.0016 0.0075 0.0048 0.0205 0.0014 0.0058 0.0003 0.0012 0.0002 0.002 0.0004
Golgi 0.0005 0 0 0.0016 0 0.0001 0.011 0.0005 0.0029 0.0034 0.0088 0.0009 0.0013 0 0.0001 0 0 0
Lipid Particles 0.0007 0.0001 0 0.0046 0.0001 0.0001 0.0132 0.0013 0.002 0.012 0.0249 0.001 0.0025 0.0001 0.0001 0 0 0.0001
Mitochondria 0.0007 0.0002 0.0001 0.0006 0.0002 0.0002 0.0043 0.0038 0.0019 0.0011 0.0065 0.0004 0.0041 0.001 0.0007 0.0003 0.0003 0.0001
None 0.0317 0.0562 0.0112 0.0139 0.006 0.0132 0.013 0.0149 0.0102 0.0006 0.0081 0.008 0.0192 0.0073 0.0388 0.0303 0.0014 0.0126
Nuclear Periphery 0.0136 0.0086 0.0057 0.0081 0.0128 0.0054 0.0152 0.0072 0.0051 0.0048 0.0402 0.0057 0.0188 0.0105 0.0085 0.0052 0.0101 0.005
Nucleolus 0.0058 0.0062 0.0048 0.0033 0.0201 0.0117 0.016 0.0061 0.0048 0.0193 0.0059 0.0093 0.0141 0.0051 0.0031 0.004 0.0043 0.0052
Nucleus 0.8894 0.8825 0.9456 0.9265 0.8936 0.9318 0.6988 0.924 0.8983 0.9184 0.8134 0.9333 0.8574 0.8933 0.888 0.9186 0.8941 0.9243
Peroxisomes 0.0001 0 0 0.0013 0 0 0.0115 0.0002 0.0009 0.0045 0.0034 0.001 0.0041 0.0002 0.0001 0 0 0
Punctate Nuclear 0.022 0.0152 0.0223 0.007 0.059 0.0261 0.0203 0.013 0.0172 0.0133 0.0132 0.0182 0.0253 0.0563 0.0376 0.0105 0.0565 0.0443
Vacuole 0.0021 0.0006 0.0001 0.0005 0.0002 0.0003 0.0069 0.0011 0.0019 0.0006 0.0032 0.0004 0.0014 0.0005 0.0003 0.0001 0.0008 0.0002
Vacuole Periphery 0.0007 0.0001 0 0.0003 0.0001 0.0001 0.0017 0.0003 0.0004 0.0003 0.0021 0.0001 0.0008 0.0002 0.0001 0 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.1582 16.5183 11.0899 11.8578 14.2605 7.9148 8.9473 10.7357 10.9277 14.3148
Translational Efficiency 0.8987 0.6561 0.9685 0.8345 0.8735 1.8349 1.235 1.052 1.0341 0.9784

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
335 442 2514 1791 845 1329 298 1238 1180 1771 2812 3029

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 711.87 635.72 784.00 773.73 694.71 726.02 875.51 870.36 699.58 703.48 793.70 813.22
Standard Deviation 107.37 102.38 94.96 95.50 84.52 148.20 114.51 124.07 91.91 143.61 101.22 118.07
Intensity Change Log 2 — -0.163222 0.139240 0.120216 — 0.063598 0.333713 0.325201 — -0.046740 0.238566 0.225098

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000136 0.000399 0.000306 0.000394 0.000041 0.000340 0.000287 0.000980 0.000068 0.000355 0.000304 0.000634
Bud Neck 0.002458 0.006221 0.011946 0.008182 0.000783 0.003271 0.005401 0.019037 0.001259 0.004007 0.011253 0.012619
Bud Site 0.001334 0.003749 0.004505 0.002908 0.000180 0.001785 0.002983 0.015256 0.000508 0.002275 0.004344 0.007955
Cell Periphery 0.000961 0.000247 0.000129 0.000098 0.000095 0.000216 0.000061 0.000172 0.000341 0.000224 0.000122 0.000128
Cytoplasm 0.056279 0.108815* 0.043990 0.020204 0.131190* 0.066932 0.013675 0.031495 0.109923* 0.077385 0.040777 0.024819
Cytoplasmic Foci 0.015302 0.015632 0.001085 0.000773 0.012998 0.013689 0.001461 0.004669 0.013652 0.014174 0.001125 0.002365
Eisosomes 0.000043 0.000048 0.000017 0.000024 0.000026 0.000032 0.000032 0.000036 0.000031 0.000036 0.000019 0.000029
Endoplasmic Reticulum 0.002337 0.003713 0.002478 0.004962 0.000861 0.002312 0.003597 0.008247 0.001280 0.002661 0.002597 0.006305
Endosome 0.000873 0.003350 0.000587 0.001008 0.000520 0.002428 0.000489 0.005540 0.000620 0.002658 0.000577 0.002860
Golgi 0.000253 0.000728 0.000180 0.000415 0.000046 0.001035 0.000030 0.002379 0.000105 0.000958 0.000164 0.001218
Lipid Particles 0.002999 0.002067 0.000524 0.000957 0.001664 0.002776 0.000748 0.001027 0.002043 0.002599 0.000548 0.000986
Mitochondria 0.003257 0.002577 0.002504 0.002843 0.000313 0.006048 0.000534 0.007230 0.001149 0.005182 0.002295 0.004636
Mitotic Spindle 0.000584 0.002787 0.002933 0.005997 0.000014 0.004287 0.000122 0.052698 0.000176 0.003913 0.002635 0.025084
None 0.005481 0.045105 0.006460 0.003721 0.007448 0.009014 0.003251 0.008695 0.006889 0.018022 0.006120 0.005754
Nuclear Periphery 0.001911 0.001003 0.001149 0.001863 0.000724 0.001350 0.000667 0.002168 0.001061 0.001264 0.001098 0.001988
Nuclear Periphery Foci 0.000408 0.000605 0.000263 0.000556 0.000288 0.000751 0.000233 0.003668 0.000322 0.000714 0.000260 0.001828
Nucleolus 0.004808 0.012095 0.003801 0.004453 0.002227 0.005712 0.001740 0.009850 0.002960 0.007305 0.003583 0.006659
Nucleus 0.879438* 0.783708* 0.913017* 0.934523* 0.837220* 0.869921* 0.959447* 0.802035* 0.849206* 0.848404* 0.917938* 0.880373*
Peroxisomes 0.003532 0.001847 0.000702 0.000371 0.000298 0.002131 0.000275 0.002399 0.001216 0.002060 0.000657 0.001200
Vacuole 0.017209 0.004698 0.003054 0.005214 0.002981 0.005052 0.004860 0.017299 0.007021 0.004964 0.003245 0.010153
Vacuole Periphery 0.000398 0.000605 0.000366 0.000532 0.000083 0.000918 0.000106 0.005120 0.000172 0.000840 0.000339 0.002407

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.80 -3.69 -7.30 -1.40 -3.44 -3.47 -7.65 -21.43 -10.75 -12.99 -4.12 -7.96 -23.40 -7.49 -12.11
Bud Neck -2.42 -9.63 -6.94 -2.53 1.88 -6.97 -2.47 -17.82 -16.26 -8.00 -5.70 -13.02 -19.00 -15.14 -6.03
Bud Site -1.91 -3.36 -2.54 0.33 1.18 -5.12 -1.61 -11.69 -10.17 -6.36 -4.47 -5.53 -11.99 -8.30 -4.76
Cell Periphery 1.57 1.88 1.92 3.82 1.42 -4.50 2.91 -4.83 2.45 -7.99 0.79 1.79 1.72 4.67 -0.74
Cytoplasm -4.91 2.67 5.75 9.88 7.50 8.25 16.50 15.19 10.07 -3.46 5.00 13.24 16.47 14.70 6.53
Cytoplasmic Foci 0.10 4.83 4.87 6.00 0.51 -0.57 8.13 7.62 8.09 -1.20 -0.34 9.82 9.44 10.66 -1.54
Eisosomes -0.42 5.39 3.07 3.09 -6.05 -2.22 -2.41 -11.09 -8.63 -3.58 -1.73 4.57 -4.99 -2.65 -12.48
Endoplasmic Reticulum -1.57 -1.43 -7.60 -3.22 -9.51 -6.45 -8.12 -18.65 -14.59 -7.13 -5.13 -8.91 -22.05 -14.34 -14.69
Endosome -2.72 1.90 -1.19 2.43 -3.57 -3.07 -0.78 -7.18 -1.75 -6.60 -3.92 0.55 -7.42 0.77 -7.70
Golgi -1.48 0.86 -0.31 0.98 -0.76 -2.33 -0.83 -3.84 -0.48 -3.80 -2.59 -0.47 -3.15 0.57 -2.79
Lipid Particles 1.99 4.89 3.89 3.47 -1.66 -2.50 2.90 2.64 4.62 -0.84 -1.24 6.56 4.39 5.24 -2.51
Mitochondria 0.54 0.63 0.42 -0.29 -0.53 -4.25 -3.08 -6.56 0.84 -6.06 -3.44 -1.53 -3.44 1.40 -2.33
Mitotic Spindle -1.36 -4.08 -5.54 -3.09 -3.69 -3.18 -1.28 -14.84 -13.91 -14.31 -3.44 -5.25 -15.39 -13.28 -14.01
None -6.67 -0.58 1.78 7.01 3.19 -1.34 3.69 -1.11 0.31 -5.15 -6.30 1.32 1.36 7.24 -0.07
Nuclear Periphery 1.23 0.85 -1.02 -6.35 -6.46 -3.76 -2.04 -12.89 -8.62 -10.74 -0.94 -1.31 -8.42 -9.46 -11.30
Nuclear Periphery Foci -0.80 1.06 -2.75 -2.45 -3.53 -1.53 -1.07 -7.73 -7.35 -4.52 -1.62 -0.73 -8.19 -7.36 -7.68
Nucleolus -4.45 0.38 -3.06 1.54 -3.88 -5.32 -1.33 -13.80 -12.68 -12.93 -6.68 -2.38 -13.96 -10.97 -12.90
Nucleus 5.33 -3.20 -3.11 -9.39 0.04 -3.01 -10.37 11.77 15.11 20.76 0.34 -9.75 3.18 2.99 15.79
Peroxisomes 0.79 1.42 1.52 4.47 0.78 -4.08 0.13 -2.88 1.08 -2.84 -1.26 1.09 0.63 3.21 -1.08
Vacuole 2.07 2.37 1.44 -2.29 -5.23 -2.53 -2.71 -9.91 -8.28 -7.15 0.86 1.84 -4.44 -7.73 -10.34
Vacuole Periphery -1.07 0.43 -0.30 0.58 -0.60 -3.39 -0.83 -3.58 -1.64 -3.52 -3.48 -1.12 -3.68 -0.58 -2.81
Standard name
Human Ortholog
Description Copper-sensing transcription factor; involved in regulation of genes required for high affinity copper transport; required for regulation of yeast copper genes in response to DNA-damaging agents; undergoes changes in redox state in response to changing levels of copper or MMS
Localization
Cell Percentages nucleus (80%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Mac1

Mac1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mac1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available