Standard name | |
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Human Ortholog | |
Description | Transcription factor involved in regulating arginine-responsive genes; acts with Arg81p and Arg82p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 5 | 1 | – | 11 | 9 | 14 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 4 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 2 | 1 | – | 5 | 8 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 7 | 3 | – | 10 | 6 | 22 | 28 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 2 | 10 | 19 | 35 | 69 | 72 | – | 61 | 42 | 50 | 50 | 23 | 51 | 76 | 7 | 3 | 3 | 0 | 1 | 1 | 0 | 1 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 3 | 2 | 12 | 1 | 32 | – | 7 | 15 | 4 | 8 | 1 | 0 | 9 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 6 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 2 |
Mitochondria | 5 | 15 | 2 | 13 | 13 | 219 | 120 | – | 310 | 280 | 285 | 325 | 2 | 16 | 1 | 6 | 5 | 1 | 2 | 0 | 3 | 1 | 3 | 11 |
Nucleus | 63 | 131 | 224 | 260 | 287 | 209 | 254 | – | 290 | 311 | 288 | 346 | 275 | 402 | 365 | 102 | 98 | 39 | 76 | 134 | 208 | 194 | 320 | 313 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 1 | 0 | – | 2 | 0 | 0 | 3 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 3 | 2 |
Nucleolus | 2 | 3 | 0 | 0 | 0 | 1 | 1 | – | 4 | 4 | 2 | 8 | 0 | 3 | 2 | 0 | 1 | 0 | 2 | 2 | 4 | 10 | 32 | 26 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 4 | 5 | 20 | 17 | 26 | – | 9 | 29 | 6 | 13 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 2 | 2 | 9 | 6 |
Vac/Vac Membrane | 0 | 6 | 2 | 2 | 23 | 9 | 23 | – | 18 | 16 | 13 | 17 | 3 | 3 | 20 | 9 | 5 | 3 | 2 | 5 | 16 | 23 | 54 | 61 |
Unique Cell Count | 69 | 143 | 237 | 289 | 368 | 415 | 466 | 534 | 561 | 507 | 582 | 303 | 463 | 468 | 118 | 110 | 46 | 89 | 148 | 246 | 239 | 448 | 442 | |
Labelled Cell Count | 73 | 158 | 245 | 301 | 392 | 540 | 535 | 728 | 720 | 690 | 806 | 309 | 480 | 478 | 127 | 116 | 51 | 89 | 148 | 246 | 239 | 448 | 442 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.2 | 6.0 | 5.6 | 5.8 | 3.9 | 4.7 | – | 3.8 | 4.1 | 4.0 | 4.2 | 6.2 | 5.5 | 6.1 | 7.8 | 7.7 | 7.8 | 5.6 | 5.7 | 5.8 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.2 | 1.8 | 1.6 | 1.5 | 1.7 | – | 0.9 | 1.0 | 0.9 | 0.9 | 1.2 | 1.2 | 1.2 | 1.2 | 1.3 | 1.4 | 1.2 | 1.4 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.06 | -0.62 | -0.36 | – | -0.65 | -0.57 | -0.6 | -0.53 | 0.04 | -0.14 | 0.03 | 0.37 | 0.36 | 0.38 | -0.11 | -0.07 | -0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.2 | 2.4 | 4.7 | 4.4 | – | 3.2 | 1.7 | 2.6 | 2.2 | 1.6 | 3.0 | 4.6 | 0.7 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.5 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.5 | 2.1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.1 | 0 | 0 | 0 | 0 |
Nucleus | -1.9 | -5.5 | -11.5 | -10.7 | – | -10.9 | -10.7 | -10.3 | -9.9 | -1.6 | -3.1 | -5.6 | -2.6 | -1.8 | -2.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2.3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.3 | 0 | 2.8 | – | 2.0 | 1.7 | 0 | 1.8 | 0 | 0 | 2.5 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6876 | 0.7937 | 0.4827 | 0.3746 | 0.1682 | 0.5923 | 1.2279 | 1.4979 | 1.2234 | 1.0282 | 1.3252 | 1.4138 | 4.1156 | 3.5081 | 3.6137 | 4.182 | 3.2159 | 3.8985 |
Actin | 0.0238 | 0 | 0.0015 | 0 | 0 | 0.0003 | 0.0102 | 0.0003 | 0.007 | 0.0082 | 0.0008 | 0.004 | 0.0096 | 0 | 0.0335 | 0.0009 | 0 | 0.0038 |
Bud | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0008 | 0.0046 | 0.0002 | 0.0001 | 0 | 0.0002 |
Bud Neck | 0.003 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0008 | 0.0012 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0008 | 0.0131 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0011 |
Bud Periphery | 0.0006 | 0 | 0 | 0 | 0 | 0.0001 | 0.0016 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0019 | 0.0042 | 0.0002 | 0.0002 | 0 | 0.0008 |
Bud Site | 0.0013 | 0 | 0 | 0 | 0 | 0.0002 | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0052 | 0.0009 | 0.0005 | 0.0002 | 0 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0009 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0058 | 0.0013 | 0.0007 | 0.0001 | 0.0002 | 0.0004 | 0.0027 | 0.0008 | 0.0009 | 0.0007 | 0.0026 | 0.0002 | 0.0082 | 0.0092 | 0.0015 | 0.0006 | 0.0005 | 0.0003 |
Cytoplasmic Foci | 0.0033 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0.0037 | 0.0001 | 0.0017 | 0.0003 | 0.0034 | 0 | 0.0185 | 0.0007 | 0.0041 | 0 | 0 | 0.0015 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0006 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0024 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0019 | 0.0002 | 0.0008 | 0.001 | 0.0009 | 0.0003 | 0.0029 | 0.0002 | 0.0007 | 0.0002 | 0 | 0.0002 |
Endosome | 0.0138 | 0 | 0.0008 | 0 | 0 | 0.0011 | 0.0076 | 0.0004 | 0.0011 | 0.0043 | 0.0301 | 0.0007 | 0.0245 | 0.0001 | 0.0047 | 0.0002 | 0 | 0.0034 |
Golgi | 0.0041 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0017 | 0.0001 | 0.0012 | 0.0011 | 0.0057 | 0.0007 | 0.0051 | 0 | 0.01 | 0 | 0 | 0.0016 |
Lipid Particles | 0.006 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0.0037 | 0.0005 | 0.0044 | 0.0005 | 0.0116 | 0.0002 | 0.0198 | 0 | 0.0039 | 0 | 0 | 0.0062 |
Mitochondria | 0.0021 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0005 | 0.0066 | 0.0002 | 0.001 | 0.0031 | 0.0058 | 0.002 | 0.0043 | 0.0003 | 0.0072 | 0.0004 | 0.0001 | 0.0049 |
None | 0.0032 | 0.0078 | 0.0049 | 0.0001 | 0.0003 | 0.0002 | 0.0045 | 0.0002 | 0.0031 | 0.0007 | 0.0079 | 0.0001 | 0.0108 | 0.0005 | 0.0086 | 0.0002 | 0.0001 | 0.0003 |
Nuclear Periphery | 0.0128 | 0.0136 | 0.0054 | 0.0005 | 0.0018 | 0.004 | 0.0113 | 0.0083 | 0.0039 | 0.0127 | 0.0071 | 0.0037 | 0.0117 | 0.004 | 0.0022 | 0.0126 | 0.0032 | 0.0035 |
Nucleolus | 0.0085 | 0.0107 | 0.0082 | 0.0046 | 0.0258 | 0.0188 | 0.0076 | 0.0064 | 0.01 | 0.0032 | 0.0288 | 0.0137 | 0.0177 | 0.006 | 0.0087 | 0.0098 | 0.0075 | 0.0169 |
Nucleus | 0.8901 | 0.9643 | 0.9611 | 0.9944 | 0.966 | 0.9687 | 0.9135 | 0.9683 | 0.9495 | 0.9608 | 0.8739 | 0.963 | 0.8111 | 0.9576 | 0.8943 | 0.9661 | 0.9833 | 0.9424 |
Peroxisomes | 0.0021 | 0 | 0.0001 | 0 | 0 | 0.0006 | 0.0015 | 0.0002 | 0.0092 | 0.0002 | 0.0011 | 0 | 0.0078 | 0 | 0.0101 | 0 | 0 | 0.0068 |
Punctate Nuclear | 0.0141 | 0.002 | 0.0158 | 0.0003 | 0.0054 | 0.0035 | 0.0098 | 0.0133 | 0.0042 | 0.0021 | 0.0138 | 0.0096 | 0.0212 | 0.0092 | 0.0079 | 0.0076 | 0.0048 | 0.004 |
Vacuole | 0.0015 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0065 | 0.0002 | 0.0003 | 0.0002 | 0.0023 | 0.0002 | 0.0037 | 0.0017 | 0.0002 | 0.0001 | 0 | 0.001 |
Vacuole Periphery | 0.0008 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0022 | 0.0002 | 0.0002 | 0.0006 | 0.0024 | 0.0004 | 0.001 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.3315 | 12.93 | 20.5599 | 14.387 | 8.0371 | 26.847 | 23.8125 | 23.3199 | 16.1207 | 15.5994 |
Translational Efficiency | 1.1435 | 0.9781 | 0.711 | 0.8791 | 1.3756 | 0.6882 | 0.4633 | 0.7803 | 0.9793 | 0.8488 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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