Standard name
Human Ortholog
Description Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments; may have a role in intracellular sterol transport

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.16 0.22 0.24 0.23 0.17 0.24 0.16 0.16 0.2 0.23 0.22 0.22 0.12 0.12 0.21 0.25 0 0 0.07 0 0.09 0.05
Bud 0 0 0.06 0.05 0.05 0.09 0.07 0.05 0.06 0.11 0.1 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0 0.06 0.05 0 0 0.05 0 0.06 0.05 0 0 0.05 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0.07 0.09 0.06 0.06 0.09 0.12 0.09 0.09 0.12 0.06 0.09 0.09 0.08 0.05 0 0.08 0 0 0 0 0.05 0.07
Endosome 0 0 0.06 0.12 0.08 0.05 0.07 0.07 0.1 0.09 0.05 0 0 0 0 0 0.15 0.15 0.15 0.12 0.1 0.11
Golgi 0.8 0.77 0.7 0.59 0.56 0.34 0.22 0.4 0.41 0.2 0.27 0.9 0.9 0.75 0.82 0.82 0.74 0.65 0.73 0.69 0.61 0.66
Mitochondria 0.18 0.11 0.59 0.51 0.48 0.65 0.63 0.36 0.44 0.6 0.62 0.11 0.07 0.15 0.16 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0.08 0.12 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0.18 0 0.05 0.05 0 0.05 0.06 0.06 0.06 0.05 0.1 0.07 0.2 0.06 0.14 0 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 35 55 38 65 84 143 105 117 144 125 170 45 24 5 14 21 7 11 11 0 4 5
Bud 6 0 9 13 25 53 46 34 44 59 80 0 3 0 2 2 0 2 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 2 1 1 3 6 6 0 0 0 0 0
Cell Periphery 0 3 1 0 3 1 3 3 5 6 9 0 1 0 1 1 0 0 0 0 0 0
Cytoplasm 4 16 5 17 27 25 27 35 27 33 36 5 6 2 5 4 1 3 0 0 0 1
Endoplasmic Reticulum 16 23 9 17 46 74 57 62 87 30 71 18 16 2 3 7 2 2 0 0 2 8
Endosome 6 1 9 34 42 29 49 47 73 49 37 2 1 0 0 1 36 40 25 2 4 12
Golgi 177 188 112 168 279 205 149 283 304 109 206 184 180 30 56 69 175 172 123 16 29 77
Mitochondria 40 26 94 144 237 395 418 259 327 326 477 22 14 6 11 5 2 5 1 0 2 1
Nucleus 0 0 0 0 1 0 0 3 3 2 0 0 0 2 0 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 3 1 3 3 1 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 10 4 4 4 16 17 53 87 32 13 40 3 0 0 1 3 0 0 0 0 0 0
Peroxisomes 0 0 0 2 6 1 3 5 3 1 2 0 0 0 0 0 2 1 2 0 0 0
SpindlePole 1 0 1 3 4 1 2 12 12 9 6 0 1 0 0 1 1 0 0 0 0 0
Vac/Vac Membrane 32 45 4 13 26 21 35 40 41 31 36 21 13 8 4 12 6 21 3 0 2 4
Unique Cell Count 220 245 159 284 498 606 667 711 736 543 774 205 199 40 68 84 237 265 170 24 48 118
Labelled Cell Count 327 361 286 480 796 970 949 991 1108 800 1177 300 259 55 97 126 237 265 170 24 48 118


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.2 21.6 12.9 12.9 11.9 11.2 10.9 11.9 11.4 11.1 11.5 20.3 20.4 24.8 27.7 27.5 20.6 21.0
Std Deviation (1e-4) 3.5 3.5 1.9 2.1 1.9 1.8 1.8 2.4 2.4 2.3 2.5 4.4 4.8 7.8 7.7 7.3 3.8 4.0
Intensity Change (Log2) -0.01 -0.12 -0.21 -0.25 -0.12 -0.18 -0.22 -0.17 0.65 0.66 0.94 1.1 1.09 0.67 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 -2.0 -0.1 -2.4 -2.2 -1.2 -0.2 -0.5 -0.4 -2.9 -1.6 -0.5 0.2
Bud -0.5 -0.3 1.3 0.6 -0.5 0.2 2.0 1.8 0 -2.2 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.2 0.6 0.5 1.0 0.3 1.4 0.8 0 0 0 0 0
Endoplasmic Reticulum 0.1 1.4 2.4 1.2 1.3 2.3 -0.1 1.4 1.1 0.9 0 0 0.8
Endosome 2.2 1.1 -0.5 0.7 0.4 1.7 1.4 -0.5 0 0 0 0 0
Golgi -2.4 -3.2 -8.3 -11.7 -7.0 -6.7 -12.0 -10.6 4.7 4.9 0.6 1.9 2.0
Mitochondria -1.7 -2.5 1.4 0.8 -5.3 -3.4 0.2 0.6 -9.8 -10.7 -5.0 -5.9 -8.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.4 3.6 1.1 0 1.4 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.1 1.4 0.6 1.5 1.6 1.6 1.6 1.2 2.9 1.8 0 0 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.7137 15.7868 15.227 14.1487 13.8695 15.8306 10.5128 14.9753 13.3803 11.9341 12.8518 13.9872 14.3351 15.9359 14.71 12.7302 14.237 14.1709
Actin 0.0063 0.0089 0.0088 0.0104 0.0026 0.0089 0.0448 0.0312 0.0279 0.0244 0.0086 0.0183 0.0357 0.0135 0.0172 0.0441 0.0149 0.0086
Bud 0.0002 0.0002 0.0001 0.0002 0 0 0.0004 0.0002 0.0002 0.0002 0.0003 0 0.0003 0.0002 0.0004 0.0001 0.0001 0.0001
Bud Neck 0.0003 0.0002 0.0002 0.0002 0.0001 0.0003 0.0006 0.0003 0.0004 0.0003 0.0003 0.0002 0.0005 0.0003 0.0003 0.0003 0.0001 0.0003
Bud Periphery 0.0002 0.0003 0.0001 0.0003 0 0 0.0004 0.0001 0.0001 0.0001 0.0004 0 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001
Bud Site 0.0003 0.0002 0.0001 0.0003 0 0.0001 0.0008 0.0002 0.0003 0.0002 0.0014 0.0001 0.0011 0.0008 0.0003 0.0001 0 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0 0 0 0
Cytoplasm 0.0013 0.003 0.001 0.0014 0.0006 0.0012 0.0035 0.0024 0.0015 0.0014 0.0007 0.0017 0.002 0.0034 0.0019 0.0008 0.0008 0.0009
Cytoplasmic Foci 0.0433 0.0728 0.0398 0.0376 0.028 0.0342 0.0657 0.0667 0.0496 0.0483 0.039 0.0245 0.0522 0.0633 0.0399 0.0387 0.03 0.0323
Eisosomes 0 0 0 0.0001 0 0 0.0005 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0006 0.0004 0.0004 0.0005 0.0004 0.0005 0.0022 0.0005 0.0005 0.0008 0.0005 0.0005 0.0006 0.0005 0.0005 0.0003 0.0003 0.0005
Endosome 0.1622 0.2009 0.1522 0.1356 0.1083 0.0968 0.1999 0.1459 0.1914 0.2962 0.2352 0.0946 0.1607 0.1845 0.1832 0.2052 0.1774 0.1008
Golgi 0.7546 0.6887 0.7847 0.7785 0.8422 0.846 0.5772 0.7088 0.6932 0.5753 0.6673 0.8542 0.7115 0.7184 0.7412 0.6957 0.7569 0.8383
Lipid Particles 0.0104 0.0078 0.0029 0.0234 0.0057 0.0043 0.0397 0.0097 0.0124 0.0365 0.0086 0.001 0.019 0.0061 0.0042 0.004 0.0024 0.0092
Mitochondria 0.0123 0.0061 0.0046 0.0048 0.0087 0.0033 0.0166 0.0037 0.0096 0.0045 0.0082 0.002 0.0053 0.0035 0.0031 0.0019 0.0096 0.0058
None 0.0001 0.0001 0 0.0005 0 0.0003 0.0015 0 0.0002 0.0003 0.0001 0.0002 0.0001 0 0 0 0 0
Nuclear Periphery 0.0003 0.0001 0.0001 0.0003 0.0002 0.0002 0.0115 0.0001 0.0004 0.0005 0.0002 0.0002 0.0005 0.0001 0.0001 0.0001 0.0001 0.0001
Nucleolus 0 0.0001 0 0.0001 0 0.0001 0.0002 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Nucleus 0.0001 0.0001 0 0.0001 0 0.0001 0.0037 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 0 0 0 0
Peroxisomes 0.0019 0.0014 0.001 0.0033 0.0002 0.0015 0.0208 0.0013 0.0079 0.002 0.0215 0.0004 0.0032 0.0016 0.001 0.0046 0.0002 0.0009
Punctate Nuclear 0.0002 0 0 0.0003 0 0.0002 0.0033 0 0.001 0.0002 0.0007 0.0001 0.0006 0 0 0 0 0
Vacuole 0.0025 0.0056 0.0014 0.001 0.0006 0.0009 0.0033 0.0193 0.0011 0.0028 0.0032 0.0007 0.0038 0.0016 0.0027 0.0014 0.0028 0.0007
Vacuole Periphery 0.0029 0.0032 0.0026 0.0012 0.0023 0.0012 0.0034 0.0094 0.002 0.0058 0.0034 0.001 0.0023 0.0018 0.0036 0.0025 0.0041 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.5555 31.3135 43.5675 52.3192 34.0622 17.5047 41.5552 43.406 54.7267 51.4461
Translational Efficiency 2.7104 2.5187 1.9028 1.6057 2.0859 5.6338 2.9297 2.0156 1.9158 1.925

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
857 1484 2718 1097 2035 1742 1186 1414 2892 3226 3904 2511

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1194.72 1540.55 2036.32 2223.31 1544.46 1459.94 2000.21 2086.45 1440.82 1497.02 2025.35 2146.24
Standard Deviation 210.87 296.02 321.48 436.18 268.95 253.37 292.58 411.18 299.30 276.74 313.42 427.71
Intensity Change Log 2 0.366773 0.769292 0.896037 -0.081193 0.373049 0.433948 0.131454 0.559372 0.653863

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.010984 0.008598 0.015413 0.084392 0.004582 0.006390 0.025730 0.062966 0.006479 0.007406 0.018547 0.072327
Bud Neck 0.000999 0.000253 0.000689 0.010338 0.000153 0.000285 0.001215 0.005977 0.000404 0.000270 0.000849 0.007883
Bud Site 0.005042 0.001886 0.001134 0.041310 0.000967 0.001654 0.002773 0.026952 0.002175 0.001760 0.001632 0.033224
Cell Periphery 0.000258 0.000135 0.000292 0.096546 0.000112 0.000112 0.000598 0.046179 0.000155 0.000122 0.000385 0.068184
Cytoplasm 0.000909 0.000803 0.000186 0.001927 0.000256 0.000739 0.000236 0.002135 0.000449 0.000768 0.000202 0.002044
Cytoplasmic Foci 0.112880 0.085634 0.030762 0.015885 0.055635 0.102386 0.022979 0.023646 0.072598 0.094680 0.028397 0.020255
Eisosomes 0.000107 0.000082 0.000257 0.013308 0.000074 0.000069 0.000303 0.004808 0.000084 0.000075 0.000271 0.008522
Endoplasmic Reticulum 0.001805 0.001021 0.003675 0.040615 0.000963 0.000946 0.004820 0.031552 0.001212 0.000980 0.004023 0.035511
Endosome 0.107152 0.073325 0.069640 0.073275 0.063296 0.105411 0.055425 0.101224 0.076292 0.090651 0.065322 0.089014
Golgi 0.689940* 0.780707* 0.769775* 0.486509* 0.827544* 0.725614* 0.803809* 0.564714* 0.786767* 0.750957* 0.780114* 0.530548*
Lipid Particles 0.016220 0.018598 0.072516 0.085664 0.008962 0.019230 0.052580 0.091736 0.011113 0.018939 0.066460 0.089083
Mitochondria 0.018532 0.006055 0.005286 0.004544 0.010637 0.009201 0.002299 0.004168 0.012977 0.007754 0.004379 0.004333
Mitotic Spindle 0.001433 0.001414 0.000371 0.001272 0.000197 0.000926 0.000495 0.000802 0.000563 0.001150 0.000409 0.001007
None 0.000083 0.000089 0.000070 0.000351 0.000044 0.000031 0.000071 0.000165 0.000056 0.000058 0.000070 0.000246
Nuclear Periphery 0.000129 0.000113 0.000410 0.000684 0.000079 0.000130 0.000289 0.000306 0.000094 0.000122 0.000373 0.000471
Nuclear Periphery Foci 0.000594 0.000444 0.000761 0.000820 0.000212 0.000679 0.000565 0.000791 0.000326 0.000571 0.000702 0.000804
Nucleolus 0.000082 0.000203 0.000009 0.000044 0.000004 0.000010 0.000008 0.000038 0.000027 0.000099 0.000009 0.000040
Nucleus 0.000048 0.000430 0.000017 0.000169 0.000007 0.000017 0.000020 0.000196 0.000019 0.000207 0.000018 0.000185
Peroxisomes 0.001681 0.000681 0.000176 0.001520 0.000237 0.000855 0.000196 0.001337 0.000665 0.000775 0.000182 0.001417
Vacuole 0.016000 0.009350 0.013034 0.029061 0.009843 0.012596 0.014160 0.019708 0.011668 0.011103 0.013376 0.023794
Vacuole Periphery 0.015122 0.010179 0.015525 0.011764 0.016197 0.012717 0.011430 0.010598 0.015878 0.011550 0.014281 0.011108

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.00 -1.84 -12.73 -13.74 -12.43 -1.55 -7.76 -14.12 -13.51 -7.64 -0.85 -8.39 -19.46 -19.21 -15.29
Bud Neck 3.13 1.11 -11.77 -13.28 -12.55 -3.27 -4.62 -8.61 -8.42 -6.62 1.73 -3.59 -14.59 -14.97 -13.51
Bud Site 3.04 4.60 -12.34 -13.65 -14.19 -2.24 -4.79 -11.63 -11.26 -10.66 0.97 1.65 -17.39 -17.54 -17.81
Cell Periphery 4.23 -1.29 -14.09 -14.10 -14.08 0.01 -4.49 -10.96 -10.96 -10.84 2.56 -6.43 -17.70 -17.71 -17.64
Cytoplasm 0.25 4.14 -2.68 -2.48 -5.08 -2.50 0.56 -3.56 -2.72 -3.59 -1.84 4.41 -4.61 -3.53 -5.25
Cytoplasmic Foci 5.85 20.23 23.81 26.24 10.91 -16.47 19.25 17.92 29.49 -0.50 -9.26 24.99 29.37 39.18 7.88
Eisosomes 1.66 -6.25 -6.95 -6.96 -6.87 0.42 -7.37 -5.28 -5.28 -5.02 0.93 -10.17 -8.65 -8.66 -8.46
Endoplasmic Reticulum 2.22 -4.33 -14.80 -15.22 -14.11 0.11 -7.31 -14.91 -14.91 -12.63 1.60 -10.18 -21.10 -21.27 -19.18
Endosome 7.23 8.34 7.13 -0.01 -1.13 -12.67 2.81 -11.05 1.10 -13.12 -5.49 4.55 -4.72 0.61 -9.42
Golgi -9.35 -8.39 14.70 23.91 23.34* 15.70 3.33 27.06 15.64 21.88* 6.74 1.35 31.98 27.72 31.18*
Lipid Particles -0.88 -14.89 -12.15 -12.09 -2.13 -5.05 -11.33 -16.64 -13.92 -6.36 -5.12 -21.09 -21.01 -18.46 -5.18
Mitochondria 4.63 5.11 5.39 1.23 0.76 0.83 6.34 4.37 3.41 -1.91 3.75 6.94 6.82 3.49 0.06
Mitotic Spindle -0.07 2.91 0.29 0.23 -3.39 -2.72 -0.97 -4.24 0.32 -0.34 -1.65 0.46 -2.73 0.37 -2.71
None -0.24 0.97 -1.70 -1.63 -1.78 3.49 -4.63 -4.46 -4.90 -3.54 -0.20 -2.73 -2.79 -2.70 -2.58
Nuclear Periphery 0.56 -5.53 -7.12 -7.32 -3.04 -2.74 -4.47 -8.83 -5.88 -0.36 -2.01 -7.63 -10.28 -9.15 -1.91
Nuclear Periphery Foci 1.06 -1.32 -1.89 -3.67 -0.43 -1.81 -4.78 -7.88 -0.67 -2.72 -1.68 -4.98 -8.11 -1.79 -1.47
Nucleolus -0.63 1.37 0.68 0.84 -3.26 -2.44 -4.26 -2.40 -1.96 -2.12 -0.82 1.11 -0.89 0.64 -3.32
Nucleus -1.29 1.55 -5.42 0.89 -13.29 -2.37 -9.35 -1.97 -1.91 -1.90 -1.39 0.05 -2.52 -0.44 -2.53
Peroxisomes 2.17 4.34 0.37 -2.06 -5.00 -3.58 0.83 -5.03 -1.76 -5.30 -0.58 4.44 -3.76 -2.70 -7.32
Vacuole 3.20 1.47 -4.86 -8.49 -7.19 -2.35 -3.06 -6.45 -4.43 -3.07 0.53 -1.80 -8.73 -9.30 -7.68
Vacuole Periphery 3.12 -0.27 2.03 -1.46 3.46 2.62 3.74 4.29 1.86 0.79 4.28 1.59 4.65 0.50 4.11
Standard name
Human Ortholog
Description Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments; may have a role in intracellular sterol transport
Localization
Cell Percentages Golgi (30%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Tvp18

Tvp18


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tvp18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available