Standard name
Human Ortholog
Description Subunit of a complex with Ctf8p; shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0 0.33 0.23 0.29 0.19 0.18 0.15 0.1 0.12 0.12 0.12 0.11 0.2 0.3 0.14 0.1 0.26 0 0 0.12 0.05 0.11 0.15
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.33 0.41 0.05 0.16 0.09 0.37 0.44 0.23 0.66 0.65 0.69 0.54 0.24 0.06 0.08 0 0.07 0.17 0 0 0 0 0 0.05
Nucleus 0.68 0.78 0.55 0.66 0.62 0.6 0.57 0.71 0.59 0.58 0.57 0.55 0.65 0.61 0.45 0.66 0.74 0.46 0.67 0.63 0.46 0.54 0.34 0.26
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0.06 0.05 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Vac/Vac Membrane 0.12 0.12 0 0 0 0 0 0 0 0 0 0 0.11 0.11 0.19 0.18 0.21 0.23 0.2 0.19 0.24 0.31 0.39 0.4
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3
Bud 0 1 0 1 0 1 2 2 5 7 9 9 0 0 2 0 0 0 0 2 2 5 13 11
Bud Neck 0 0 8 1 6 1 4 11 1 7 2 3 0 0 0 0 0 0 0 0 0 1 4 8
Bud Site 0 0 0 0 0 1 13 2 5 9 9 22 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 1 62 37 71 55 75 59 31 40 47 51 31 91 126 8 4 9 3 2 31 10 48 56
Endoplasmic Reticulum 1 0 3 0 0 1 0 0 0 0 1 0 0 2 1 1 1 3 0 0 0 1 0 3
Endosome 0 0 0 0 1 2 2 0 0 0 0 0 5 24 15 1 0 1 0 2 3 0 5 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 6 1
Mitochondria 41 24 9 25 21 108 185 90 209 224 271 233 68 27 32 1 3 6 0 0 8 5 17 18
Nucleus 86 46 103 107 151 173 236 282 187 200 225 238 188 279 193 37 31 16 91 52 118 107 150 98
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1 1 0 0 0 0 0 0 1 1
Nucleolus 2 0 0 0 0 0 3 0 1 3 1 2 12 21 18 1 1 0 4 1 6 0 2 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 1 0 9 9 13 4 20 21 8 8 6 16 2 6 3 0 0 0 4 3 16 1 6 11
Vac/Vac Membrane 15 7 6 2 5 9 8 13 10 3 12 17 33 51 79 10 9 8 27 16 61 62 169 150
Unique Cell Count 126 59 186 161 245 289 416 399 315 347 392 435 289 459 426 56 42 35 137 84 258 199 435 375
Labelled Cell Count 155 79 201 182 268 355 548 482 457 502 583 592 339 501 471 60 49 43 137 84 258 199 435 375


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.8 4.8 4.4 4.8 3.8 4.0 4.4 3.5 3.3 3.4 3.8 4.4 4.5 4.4 6.9 6.3 7.2 4.0 4.0 4.4
Std Deviation (1e-4) 0.5 0.5 1.0 1.3 1.1 0.9 1.1 1.1 0.9 1.0 1.0 0.8 0.9 0.9 1.2 1.9 1.0 2.1 1.0 1.3 1.5
Intensity Change (Log2) – – – -0.12 -0.0 -0.32 -0.27 -0.12 -0.44 -0.52 -0.51 -0.34 -0.13 -0.11 -0.11 0.52 0.4 0.59 -0.25 -0.25 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6376 -0.586 -0.5708 0.48 -0.1914 -0.4946 -0.4827 -0.0179 0.0883 -0.4881 -0.0102 -0.255 -0.6268 -0.1273 0.0032 -0.1746 -0.1521 -0.3707
Actin 0.0861 0.0007 0.0044 0.1042 0.0068 0.0054 0.0055 0.0001 0.0089 0 0.0012 0.0003 0.0386 0.0001 0.0193 0.0005 0.002 0.0018
Bud 0.0022 0.0002 0.0002 0.0015 0.0003 0.0002 0.0011 0.0001 0.0003 0 0.0003 0.0001 0.0005 0.0001 0.0005 0 0.0002 0.0001
Bud Neck 0.003 0.001 0.0006 0.0038 0.0003 0.0028 0.0102 0.0004 0.001 0.0002 0.001 0.0018 0.0022 0.0003 0.0061 0.0003 0.0014 0.0015
Bud Periphery 0.0046 0.0003 0.0005 0.0016 0.001 0.0005 0.0024 0.0002 0.0006 0 0.0005 0.0002 0.0009 0.0001 0.0007 0 0.0002 0.0001
Bud Site 0.006 0.0101 0.0038 0.0153 0.0005 0.0015 0.0094 0.0004 0.0018 0 0.0005 0.0002 0.0019 0.0004 0.0041 0.0002 0.0008 0.0001
Cell Periphery 0.0005 0.0003 0.0001 0.0004 0.0002 0.0001 0.0008 0 0.0003 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0
Cytoplasm 0.0285 0.011 0.0055 0.0066 0.0006 0.016 0.0199 0.0174 0.0071 0.001 0.0132 0.0088 0.0132 0.0063 0.0077 0.0015 0.0051 0.0041
Cytoplasmic Foci 0.0218 0.0006 0.0033 0.0671 0.0086 0.0044 0.0223 0.0002 0.0112 0 0.004 0.0012 0.0052 0.0022 0.0156 0.01 0.007 0.0037
Eisosomes 0.0012 0.0001 0.0001 0.0005 0.0003 0.0001 0.0001 0 0.0001 0 0 0 0.0005 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0103 0.0018 0.0076 0.0046 0.0012 0.0008 0.0035 0.0022 0.0014 0 0.0029 0.0008 0.0029 0.0003 0.0005 0.0001 0.0005 0.0002
Endosome 0.0285 0.002 0.0033 0.0903 0.0471 0.0105 0.0531 0.0006 0.023 0.0001 0.0248 0.0032 0.0151 0.0003 0.0104 0.0137 0.0319 0.0017
Golgi 0.0138 0.0005 0.0013 0.0286 0.0197 0.0103 0.0099 0 0.0078 0 0.006 0.0003 0.0095 0 0.0079 0.0086 0.0203 0.001
Lipid Particles 0.0179 0.0014 0.0018 0.0524 0.1112 0.0056 0.0313 0 0.0067 0 0.0325 0.0009 0.0118 0.0003 0.0072 0.0168 0.0103 0.0055
Mitochondria 0.0315 0.005 0.0038 0.0108 0.1855 0.002 0.0161 0.0004 0.0031 0.0003 0.0294 0.0004 0.0156 0.0002 0.0032 0.001 0.0887 0.0003
None 0.032 0.0264 0.0198 0.0025 0.0005 0.016 0.0249 0.0219 0.0091 0.0002 0.0444 0.0188 0.0347 0.0995 0.0297 0.0526 0.0008 0.002
Nuclear Periphery 0.036 0.0224 0.0176 0.0076 0.0104 0.0086 0.0205 0.0177 0.021 0.0036 0.023 0.0117 0.0162 0.012 0.0086 0.0026 0.004 0.0038
Nucleolus 0.0097 0.0162 0.0306 0.0038 0.0012 0.0166 0.0171 0.0159 0.0327 0.01 0.0231 0.0276 0.0121 0.0145 0.0157 0.0021 0.0073 0.0195
Nucleus 0.592 0.8769 0.8562 0.5538 0.5922 0.8581 0.6959 0.9109 0.8377 0.977 0.7639 0.9002 0.7891 0.8499 0.843 0.8837 0.7848 0.9198
Peroxisomes 0.0279 0.0003 0.0051 0.0305 0.0055 0.0019 0.0101 0 0.0078 0 0.0103 0.0005 0.0033 0 0.0071 0.0033 0.0023 0.0073
Punctate Nuclear 0.038 0.0201 0.0321 0.0074 0.0027 0.0373 0.0319 0.0103 0.0126 0.0073 0.0132 0.0223 0.0228 0.0131 0.0108 0.0024 0.0297 0.027
Vacuole 0.0054 0.0019 0.0011 0.0047 0.0018 0.0009 0.0116 0.001 0.0048 0.0001 0.0033 0.0004 0.0021 0.0003 0.0012 0.0003 0.0016 0.0004
Vacuole Periphery 0.0027 0.0006 0.001 0.0019 0.0023 0.0003 0.0025 0.0003 0.001 0 0.0024 0.0002 0.0016 0.0001 0.0004 0.0002 0.0008 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.2284 11.521 7.5627 16.1879 29.8848 24.7707 11.4249 11.0373 11.7596 28.5406
Translational Efficiency 0.7798 0.7048 0.5393 0.5021 0.5122 0.8714 0.5571 0.4497 0.6044 0.5452

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1400 1473 78 1289 1674 1320 484 1999 3074 2793 562 3288

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 613.14 746.27 994.80 838.62 679.88 743.52 909.88 845.20 649.48 744.97 921.67 842.62
Standard Deviation 80.31 100.75 135.86 110.49 84.49 96.91 96.45 115.74 89.05 98.96 106.93 113.76
Intensity Change Log 2 — 0.283481 0.698190 0.451801 — 0.129091 0.420396 0.314013 — 0.204364 0.558804 0.380993

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000021 0.000199 0.001809 0.001535 0.000219 0.000269 0.000494 0.000362 0.000129 0.000232 0.000676 0.000822
Bud Neck 0.001669 0.006195 0.004015 0.018366 0.002339 0.009818 0.005030 0.011719 0.002034 0.007907 0.004889 0.014325
Bud Site 0.000487 0.002236 0.001645 0.024419 0.001337 0.003191 0.001607 0.008144 0.000950 0.002687 0.001612 0.014524
Cell Periphery 0.000174 0.000131 0.000153 0.000199 0.000127 0.000182 0.000080 0.000120 0.000149 0.000155 0.000090 0.000151
Cytoplasm 0.321437* 0.144401* 0.015389 0.100645* 0.174867* 0.163194* 0.030804 0.057976 0.241620* 0.153283* 0.028665 0.074704
Cytoplasmic Foci 0.045585 0.026161 0.001206 0.004595 0.024805 0.046457 0.001209 0.001293 0.034269 0.035753 0.001208 0.002587
Eisosomes 0.000056 0.000036 0.000066 0.000035 0.000035 0.000061 0.000039 0.000019 0.000044 0.000048 0.000043 0.000025
Endoplasmic Reticulum 0.000375 0.001982 0.016256 0.007297 0.001377 0.002035 0.006524 0.004441 0.000921 0.002007 0.007874 0.005560
Endosome 0.001230 0.002376 0.000754 0.005609 0.001590 0.002905 0.000660 0.001564 0.001426 0.002626 0.000673 0.003150
Golgi 0.000427 0.000795 0.000113 0.002455 0.000291 0.001250 0.000055 0.000558 0.000353 0.001010 0.000063 0.001302
Lipid Particles 0.002909 0.003551 0.001028 0.000849 0.003154 0.007580 0.000720 0.000367 0.003043 0.005455 0.000763 0.000556
Mitochondria 0.000973 0.002715 0.001074 0.008027 0.002949 0.007609 0.000678 0.003419 0.002049 0.005028 0.000733 0.005226
Mitotic Spindle 0.000264 0.002927 0.002436 0.038242 0.003062 0.004822 0.000700 0.013297 0.001788 0.003823 0.000941 0.023076
None 0.030907 0.011637 0.001067 0.010802 0.014217 0.008284 0.008155 0.007220 0.021818 0.010053 0.007171 0.008624
Nuclear Periphery 0.000518 0.001352 0.000998 0.003826 0.001047 0.002044 0.001114 0.002234 0.000806 0.001679 0.001098 0.002858
Nuclear Periphery Foci 0.000380 0.001185 0.000447 0.001498 0.001074 0.000988 0.000330 0.000814 0.000758 0.001092 0.000346 0.001082
Nucleolus 0.005005 0.010040 0.002289 0.001877 0.007460 0.007655 0.002162 0.001227 0.006342 0.008913 0.002180 0.001482
Nucleus 0.582154* 0.772613* 0.936028* 0.727832* 0.753734* 0.716217* 0.929357* 0.872642* 0.675591* 0.745960* 0.930283* 0.815872*
Peroxisomes 0.000957 0.003386 0.000384 0.000979 0.001437 0.006096 0.000230 0.000141 0.001218 0.004667 0.000251 0.000469
Vacuole 0.004236 0.005506 0.012648 0.035396 0.004471 0.008396 0.009923 0.010938 0.004364 0.006872 0.010301 0.020526
Vacuole Periphery 0.000238 0.000575 0.000195 0.005518 0.000407 0.000945 0.000129 0.001507 0.000330 0.000750 0.000139 0.003079

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.80 -7.55 -6.30 -5.15 3.49 -0.56 -4.54 -2.91 -4.47 3.31 -2.24 -8.91 -7.15 -6.63 2.14
Bud Neck -6.07 -3.58 -12.55 -7.38 -5.23 -6.50 -4.06 -12.06 -3.71 -6.15 -8.78 -5.14 -17.27 -7.78 -8.03
Bud Site -2.81 -4.08 -7.25 -5.86 -5.63 -2.44 -1.38 -4.54 -2.17 -3.34 -3.53 -2.66 -8.52 -5.89 -6.33
Cell Periphery 3.20 -0.71 1.44 -1.56 1.36 -2.74 3.57 3.00 4.35 -0.49 -0.52 4.66 3.55 2.98 -1.35
Cytoplasm 20.41 30.41 29.08 9.14 -8.37 1.54 24.40 21.49 18.94 -6.18 15.26 40.61 35.52 20.59 -10.73
Cytoplasmic Foci 6.20 17.44 17.03 11.68 -2.15 -5.90 13.67 13.86 15.62 0.93 -0.30 22.20 21.92 19.59 -1.41
Eisosomes 4.15 -2.31 0.44 -4.83 2.59 -4.79 -2.63 3.71 7.36 4.90 -1.49 -2.07 3.45 4.66 3.97
Endoplasmic Reticulum -9.55 -8.83 -22.05 -17.67 3.93 -2.71 -11.17 -19.69 -12.76 3.95 -7.72 -14.12 -29.43 -21.89 3.57
Endosome -1.94 1.04 -3.45 -0.65 -4.37 -2.40 2.99 1.88 3.84 -1.05 -2.90 3.04 -1.12 2.10 -4.12
Golgi -0.93 1.28 -1.29 -0.22 -2.43 -2.89 5.09 0.48 2.95 -2.14 -2.60 2.99 -0.89 1.94 -3.31
Lipid Particles -1.66 6.12 7.29 6.68 0.55 -5.16 7.58 9.19 9.16 4.51 -5.17 10.28 11.92 11.43 2.62
Mitochondria -4.01 -1.62 -3.62 -1.47 -3.01 -3.69 2.44 0.22 4.58 -3.99 -4.43 2.29 -1.93 2.77 -5.32
Mitotic Spindle -3.47 -1.22 -8.85 -7.62 -4.03 -1.39 1.64 -5.29 -3.93 -6.92 -2.68 -0.07 -10.22 -8.44 -9.79
None 6.79 11.30 8.38 3.35 -7.25 4.58 4.53 6.37 1.99 -0.16 7.93 8.90 10.47 4.24 -1.03
Nuclear Periphery -6.02 -4.03 -10.06 -8.01 -7.59 -3.71 -4.71 -13.13 -4.43 -9.37 -6.14 -7.23 -15.60 -9.65 -11.36
Nuclear Periphery Foci -2.93 -0.99 -5.50 0.76 -4.89 1.58 3.07 1.31 -0.01 -1.23 -1.34 2.28 -0.62 0.80 -2.63
Nucleolus -3.10 3.23 3.52 7.58 0.53 0.98 5.99 5.93 6.03 -0.02 -1.59 6.62 6.79 9.58 0.16
Nucleus -17.30 -23.21 -14.41 1.95 11.93 3.45 -19.33 -14.74 -17.01 7.04 -9.14 -32.58 -21.33 -11.59 14.52
Peroxisomes -3.82 2.43 2.31 4.94 0.01 -4.81 3.46 3.80 6.81 2.17 -5.98 4.42 4.54 8.50 0.37
Vacuole -3.35 -4.41 -15.28 -14.71 -9.55 -4.24 -5.49 -9.17 -6.58 -0.38 -5.23 -6.36 -17.49 -15.82 -6.67
Vacuole Periphery -1.45 -0.02 -2.96 -2.27 -2.98 -2.08 2.81 -1.06 0.59 -2.05 -2.42 2.59 -3.03 -1.44 -3.70
Standard name
Human Ortholog
Description Subunit of a complex with Ctf8p; shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint
Localization
Cell Percentages nucleus (52%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ctf18

Ctf18


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ctf18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available