Standard name
Human Ortholog
Description Protein involved in directionality of mating type switching; acts with Fkh1p to control which donor mating-type locus is inserted into MAT locus during mating type switching; localized to the nucleus; not an essential gene
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.09 0.24 0.23 0.13 0.09 0.12 0.12 0.09 0.15 0.09 0.17 0.21 0.32 0.08 0.12 0.11 0 0 0 0.56 0.78 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0 0 0.11 0.06 0.29 0.55 0.34 0.68 0.51 0.74 0.44 0 0 0 0.06 0 0.06 0 0 0 0.05 0 0
Nucleus 0.75 0.84 0.86 0.66 0.61 0.67 0.59 0.67 0.5 0.59 0.34 0.5 0.78 0.73 0.59 0.79 0.75 0.8 0.7 0.74 0.65 0.19 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.05 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Vac/Vac Membrane 0.1 0.13 0 0 0.11 0.07 0 0 0 0 0.07 0.09 0 0.05 0.08 0.1 0.1 0.07 0.1 0.09 0.12 0.08 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 7 6
Bud 1 0 0 0 0 2 6 5 1 2 1 2 0 1 0 0 0 2 6 5 6 5 19 14
Bud Neck 0 0 2 1 1 2 1 2 0 1 2 1 0 0 0 0 0 0 1 0 1 3 4 4
Bud Site 0 0 0 0 1 4 10 8 7 8 8 2 0 0 0 1 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 3 5
Cytoplasm 9 11 16 46 66 29 24 36 25 23 23 19 72 156 214 33 53 40 4 3 7 185 506 489
Endoplasmic Reticulum 0 0 3 0 1 0 0 0 0 0 0 1 4 0 2 9 15 9 0 0 0 3 6 4
Endosome 0 1 1 1 9 2 3 0 0 2 1 1 3 10 9 8 8 6 4 2 3 4 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 4 3 0 2 1 1 3 1
Mitochondria 69 3 2 20 18 65 153 99 146 129 111 95 12 4 2 23 9 24 2 2 5 14 29 17
Nucleus 190 180 160 126 174 152 164 195 108 149 51 110 332 539 394 310 332 302 189 174 154 61 10 1
Nuclear Periphery 0 1 1 0 0 2 2 0 0 1 0 2 0 1 0 3 2 0 0 0 1 1 1 2
Nucleolus 10 6 0 0 2 1 7 7 4 4 3 4 0 0 1 9 6 7 18 12 11 1 1 0
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 2 1
SpindlePole 1 0 6 9 9 2 2 10 2 6 1 5 8 7 10 2 2 1 12 9 10 12 15 9
Vac/Vac Membrane 26 29 5 8 31 15 7 9 5 10 10 20 13 40 52 41 45 27 27 20 28 25 19 4
Unique Cell Count 253 215 187 190 284 226 278 292 216 251 151 218 427 739 663 391 445 379 271 236 238 332 649 581
Labelled Cell Count 308 231 196 211 312 276 379 371 298 335 211 262 445 762 685 442 477 422 271 236 238 332 649 581


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.9 5.8 4.9 5.2 4.3 4.1 4.7 3.8 4.3 3.6 4.2 5.6 5.9 5.7 7.0 8.0 7.5 5.0 5.0 5.4
Std Deviation (1e-4) 0.7 1.1 1.1 1.1 1.8 1.0 1.2 1.4 1.0 1.2 0.9 0.7 1.2 1.2 1.4 1.2 1.7 1.6 1.6 1.5 1.8
Intensity Change (Log2) – – – -0.24 -0.15 -0.43 -0.49 -0.3 -0.59 -0.42 -0.66 -0.47 -0.05 0.02 -0.02 0.27 0.47 0.39 -0.19 -0.2 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4.1 4.1 1.4 0 1.3 1.0 0.2 1.9 0.1 2.7 3.9 6.4 0 1.2 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.8 0 0 0 0 0 0 0 0 0 0 2.7 0 2.8
Nucleus -4.4 -5.7 -4.3 -6.1 -4.6 -7.5 -5.9 -9.8 -7.5 -2.2 -3.6 -6.6 -1.8 -3.0 -1.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.8 3.3 1.9 0 0.3 0 0.7 1.8 2.7 0.3 1.6 2.5 3.2 3.2 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8744 1.2817 1.3373 0.8507 1.0532 1.2003 1.4049 1.4845 1.3626 1.8284 1.4315 1.4567 1.5659 1.4472 1.5567 2.1254 2.2679 1.3495
Actin 0.0001 0 0 0 0 0.0001 0.0238 0.0001 0.0021 0 0.0029 0.0013 0.0327 0.0001 0.0008 0.0038 0.0391 0.0069
Bud 0.0001 0 0 0 0 0 0.0005 0.0002 0.0005 0 0.0001 0.0001 0.0007 0.0004 0.0002 0.0007 0.0009 0.0002
Bud Neck 0.0027 0.0002 0 0.0001 0.001 0.0008 0.0042 0.0001 0.0017 0.0001 0.0015 0.0011 0.0019 0.0002 0.0006 0.0009 0.0009 0.001
Bud Periphery 0.0003 0 0 0 0 0 0.0008 0.0001 0.001 0 0.0002 0.0003 0.0012 0.0004 0.0003 0.0014 0.0025 0.0007
Bud Site 0.0002 0 0 0 0 0 0.0087 0.0003 0.0031 0 0.0001 0.0004 0.0045 0.0005 0.0012 0.003 0.0009 0.0008
Cell Periphery 0.0001 0 0 0 0 0 0.0002 0 0.0002 0 0 0 0.0002 0 0.0001 0.0004 0.0001 0.0001
Cytoplasm 0.0005 0.0024 0.0001 0.0004 0.0002 0.0002 0.0043 0.0088 0.0064 0.0001 0.0005 0.0003 0.0081 0.0064 0.0034 0.0039 0.0466 0.0005
Cytoplasmic Foci 0.0001 0.0003 0.0012 0 0 0.0001 0.0203 0.0006 0.0027 0 0.0003 0.0018 0.0146 0.0022 0.0004 0.0108 0.0004 0.0009
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0 0 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0001 0.0001 0 0 0 0 0.0017 0.0006 0.0011 0 0.0008 0.0006 0.0068 0.0003 0.0006 0.0016 0.0011 0.0009
Endosome 0.0002 0.0002 0.0003 0 0.0001 0.0001 0.0198 0.0005 0.0096 0 0.0041 0.0045 0.0172 0.0064 0.0019 0.0265 0.0054 0.0042
Golgi 0 0 0.0001 0 0 0 0.004 0 0.0018 0 0.0007 0.0017 0.0059 0.0006 0.0001 0.0029 0.0031 0.0024
Lipid Particles 0.0001 0 0.0015 0 0 0.0001 0.0129 0.0001 0.0044 0 0.0021 0.0155 0.0163 0.0002 0.0002 0.0043 0.0011 0.0069
Mitochondria 0.0013 0.0001 0.0001 0.0001 0.0001 0 0.0041 0.0003 0.0142 0.0001 0.0017 0.0041 0.0041 0.0016 0.0005 0.0031 0.0147 0.0048
None 0.0005 0.002 0.0002 0 0.0001 0.0001 0.0036 0.0145 0.0006 0 0.0004 0.0002 0.0024 0.0057 0.0006 0.0014 0.0007 0.001
Nuclear Periphery 0.0029 0.0047 0.0079 0.0029 0.0116 0.0012 0.0343 0.0135 0.007 0.0004 0.0532 0.0063 0.0331 0.0033 0.0042 0.0251 0.028 0.01
Nucleolus 0.0199 0.018 0.0126 0.0115 0.1255 0.0299 0.0182 0.0142 0.0259 0.0157 0.0642 0.0312 0.021 0.0153 0.0197 0.0187 0.0739 0.0293
Nucleus 0.9516 0.9539 0.9574 0.9707 0.8031 0.9455 0.7994 0.9321 0.8996 0.983 0.8487 0.9192 0.7961 0.9439 0.9584 0.8592 0.7633 0.9233
Peroxisomes 0 0 0.0015 0 0 0.0001 0.006 0 0.0034 0 0.0005 0.0024 0.0088 0.0003 0.0002 0.0007 0.0003 0.0006
Punctate Nuclear 0.0188 0.0176 0.017 0.0141 0.058 0.0218 0.0273 0.0132 0.0105 0.0005 0.016 0.0081 0.0168 0.0111 0.0023 0.0111 0.012 0.0034
Vacuole 0.0003 0.0004 0 0 0.0001 0 0.0034 0.0006 0.003 0 0.0007 0.0005 0.0028 0.0008 0.0037 0.0174 0.0013 0.001
Vacuole Periphery 0.0002 0.0001 0 0 0.0001 0 0.0023 0.0002 0.0012 0 0.0012 0.0005 0.0046 0.0005 0.0006 0.0031 0.0035 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.1992 16.2958 7.1131 10.0673 18.769 16.0757 11.3883 12.429 10.2439 18.756
Translational Efficiency 1.5217 1.508 1.2808 1.2338 1.4141 2.6999 2.1531 1.595 1.6267 1.6211

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
925 1110 2304 1078 1949 1549 1667 909 2874 2659 3971 1987

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 637.75 693.54 851.23 932.73 672.57 759.34 861.33 962.05 661.36 731.87 855.47 946.14
Standard Deviation 71.79 101.83 110.14 127.48 76.88 93.89 113.36 122.85 77.02 102.55 111.61 126.23
Intensity Change Log 2 — 0.120988 0.416558 0.548469 — 0.175062 0.356882 0.516427 — 0.148996 0.386235 0.532111

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000276 0.000943 0.000578 0.001467 0.000173 0.000399 0.000823 0.001429 0.000206 0.000626 0.000681 0.001449
Bud Neck 0.002591 0.006666 0.021286 0.026901 0.004374 0.009960 0.015080 0.024285 0.003800 0.008585 0.018681 0.025704
Bud Site 0.000741 0.002505 0.002005 0.004200 0.000741 0.002932 0.001587 0.008897 0.000741 0.002754 0.001829 0.006348
Cell Periphery 0.000220 0.000299 0.000060 0.000131 0.000101 0.000111 0.000100 0.000155 0.000139 0.000189 0.000076 0.000142
Cytoplasm 0.071623 0.052720 0.002023 0.008428 0.045112 0.027112 0.008064 0.014140 0.053645 0.037802 0.004559 0.011041
Cytoplasmic Foci 0.025712 0.012764 0.000016 0.001036 0.005858 0.004581 0.000135 0.000747 0.012248 0.007997 0.000066 0.000904
Eisosomes 0.000057 0.000045 0.000021 0.000030 0.000022 0.000028 0.000027 0.000039 0.000033 0.000035 0.000024 0.000034
Endoplasmic Reticulum 0.002060 0.005313 0.004225 0.007837 0.001680 0.003400 0.004750 0.008913 0.001802 0.004198 0.004445 0.008329
Endosome 0.000922 0.001826 0.000192 0.001302 0.000558 0.001999 0.000387 0.002638 0.000675 0.001927 0.000274 0.001913
Golgi 0.000564 0.001388 0.000073 0.002084 0.000277 0.000962 0.000114 0.002834 0.000369 0.001140 0.000090 0.002427
Lipid Particles 0.008028 0.003393 0.000062 0.000282 0.001261 0.001615 0.000132 0.000447 0.003439 0.002357 0.000091 0.000358
Mitochondria 0.006766 0.004462 0.001521 0.004650 0.001349 0.005713 0.001636 0.007915 0.003093 0.005191 0.001570 0.006144
Mitotic Spindle 0.000840 0.002575 0.002215 0.010305 0.001805 0.011287 0.002765 0.031150 0.001494 0.007650 0.002446 0.019841
None 0.033849 0.030671 0.001915 0.004219 0.010680 0.006786 0.006271 0.005711 0.018137 0.016757 0.003744 0.004901
Nuclear Periphery 0.002241 0.001235 0.000713 0.002872 0.001650 0.002140 0.000936 0.005706 0.001840 0.001763 0.000806 0.004168
Nuclear Periphery Foci 0.001445 0.002696 0.000126 0.000886 0.000758 0.001052 0.000344 0.001129 0.000979 0.001738 0.000218 0.000997
Nucleolus 0.036875 0.034579 0.012263 0.007675 0.021927 0.022803 0.024108 0.008237 0.026738 0.027719 0.017235 0.007932
Nucleus 0.800263* 0.828334* 0.949150* 0.902366* 0.899806* 0.890222* 0.929628* 0.851611* 0.867768* 0.864387* 0.940955* 0.879147*
Peroxisomes 0.003270 0.003727 0.000123 0.000316 0.000635 0.001791 0.000302 0.000491 0.001483 0.002599 0.000198 0.000396
Vacuole 0.001312 0.002930 0.001268 0.009858 0.001057 0.004065 0.002397 0.020766 0.001139 0.003591 0.001742 0.014848
Vacuole Periphery 0.000346 0.000928 0.000165 0.003157 0.000176 0.001044 0.000414 0.002759 0.000231 0.000996 0.000270 0.002975

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.74 -11.01 -11.56 -4.93 -7.72 -6.33 -19.28 -15.40 -12.39 -7.44 -11.03 -22.68 -18.88 -12.13 -10.78
Bud Neck -6.39 -19.21 -16.12 -13.44 -3.61 -6.02 -14.73 -12.52 -8.95 -3.74 -7.79 -22.78 -19.84 -15.61 -4.95
Bud Site -4.94 -5.64 -7.87 -3.51 -4.13 -3.37 -4.82 -6.13 -4.14 -5.20 -4.98 -6.52 -8.78 -5.11 -6.48
Cell Periphery -1.81 10.16 5.44 4.28 -6.73 -0.67 0.64 -2.72 -1.50 -2.78 -2.52 7.03 0.97 3.07 -6.57
Cytoplasm 3.32 16.60 14.55 12.04 -5.94 5.38 13.75 7.61 2.24 -5.70 5.25 22.34 16.34 10.76 -8.15
Cytoplasmic Foci 4.92 10.39 10.19 6.82 -1.99 1.43 7.82 7.02 5.99 -2.19 4.04 12.73 12.17 8.83 -2.94
Eisosomes 3.58 11.50 6.12 2.22 -9.03 -3.20 -4.26 -11.52 -8.19 -9.50 -0.90 5.54 -5.94 -4.77 -13.10
Endoplasmic Reticulum -9.91 -9.80 -17.52 -8.35 -10.23 -7.49 -12.75 -20.30 -15.46 -12.12 -12.45 -16.28 -27.12 -17.43 -15.79
Endosome -3.42 8.08 0.07 3.17 -4.84 -3.58 2.00 -3.81 1.02 -4.59 -4.82 6.63 -3.49 2.55 -6.59
Golgi -1.75 4.58 -0.32 0.70 -1.22 -2.00 1.08 -0.89 0.11 -1.16 -2.77 2.89 -0.99 0.52 -1.68
Lipid Particles 4.62 10.23 9.91 4.00 -7.53 -1.76 8.63 5.17 5.37 -9.91 2.72 12.26 11.02 5.81 -12.46
Mitochondria 1.77 4.49 2.28 0.49 -3.87 -5.21 -1.28 -3.17 0.99 -2.84 -2.96 3.54 -1.61 1.25 -4.54
Mitotic Spindle -2.72 -2.80 -4.95 -3.69 -3.59 -4.44 -2.02 -6.90 -4.31 -6.36 -4.84 -2.72 -8.22 -4.90 -7.28
None 1.05 12.13 11.35 10.89 -5.69 3.67 4.73 6.25 2.49 0.70 0.99 13.76 13.09 10.93 -2.07
Nuclear Periphery 1.56 2.14 -1.68 -6.98 -8.59 -1.79 2.66 -3.58 -3.00 -4.25 0.20 3.64 -4.50 -4.94 -6.91
Nuclear Periphery Foci -2.24 5.36 2.54 4.17 -4.01 -1.43 1.86 -1.47 -0.28 -2.52 -2.83 4.92 -0.19 2.43 -4.00
Nucleolus 0.83 7.58 10.11 10.48 4.55 -0.51 -2.53 8.92 8.33 10.00 -0.50 3.98 13.15 13.09 10.24
Nucleus -3.08 -17.39 -10.46 -7.72 8.30 1.72 -3.77 7.60 5.83 10.02 0.49 -15.47 -0.19 -0.63 13.09
Peroxisomes -0.12 7.28 7.00 3.88 -4.50 -3.27 2.62 2.65 4.50 0.30 -2.20 7.84 7.38 5.52 -2.50
Vacuole -3.34 -1.04 -6.31 -5.36 -6.15 -4.67 -3.49 -9.52 -8.53 -8.28 -5.68 -3.71 -11.35 -9.88 -10.29
Vacuole Periphery -1.56 3.76 -1.67 -0.64 -1.96 -3.12 -1.18 -2.00 0.27 -1.41 -3.41 -0.23 -2.49 -0.41 -2.41
Standard name
Human Ortholog
Description Protein involved in directionality of mating type switching; acts with Fkh1p to control which donor mating-type locus is inserted into MAT locus during mating type switching; localized to the nucleus; not an essential gene
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Fdo1

Fdo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fdo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available