Standard name
Human Ortholog
Description Protein of unknown function with possible role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.15 0 0.08 0.06 0.08
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0
Endosome 0.07 0.05 0 0 0.13 0.05 0.11 0 0 0 0 0.06 0.07 0.19 0 0 0.24 0.21 0.28 0.22 0.24 0.22
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.1 0.12 0.09 0.1 0.16 0.14
Mitochondria 0.77 0.8 0.8 0.58 0.39 0.6 0.64 0.82 0.81 0.88 0.85 0.8 0.61 0.43 0.11 0.06 0.11 0.23 0.06 0.1 0.09 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.12 0.08 0 0 0 0.05 0.07 0.07 0.08 0.11 0.08 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.25 0.17 0.15 0.19 0.15 0.23 0.21 0.26 0.24 0.25 0.23 0.15 0.16 0.18 0.61 0.88 0 0 0 0 0 0
Peroxisomes 0.25 0.18 0.27 0.42 0.56 0.37 0.16 0.06 0 0 0 0.21 0.52 0.55 0 0.06 0.35 0.19 0.42 0.38 0.36 0.39
SpindlePole 0.08 0 0.12 0.13 0.16 0.09 0.07 0 0 0 0 0.05 0.08 0.07 0 0.06 0.06 0 0.06 0 0.05 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.12 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 1 9 1 7 1 7 2 0 1 1 2 6 0 0 0 18 12 6 5 22 34
Bud 2 1 4 8 15 15 17 12 11 9 8 2 5 0 0 0 1 0 0 1 5 5
Bud Neck 0 0 0 1 1 0 0 0 1 0 0 1 1 0 0 0 0 1 0 0 0 0
Bud Site 2 0 0 2 1 6 4 8 9 13 3 0 1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 1 2 0 0 0 0 0 0 0 0
Cytoplasm 3 1 1 7 26 8 24 6 7 10 13 2 2 6 6 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 1 1 2 9 3 3 1 1 0 0 1 2 0 0 0 0 0 0
Endosome 17 3 2 9 72 29 104 6 13 1 13 13 21 63 1 0 59 17 41 16 85 87
Golgi 1 0 0 0 0 0 7 3 2 0 3 0 0 2 4 1 23 9 12 7 56 55
Mitochondria 176 52 105 151 222 324 610 630 711 563 641 173 196 146 6 1 25 19 8 7 31 36
Nucleus 2 0 0 1 0 2 9 3 9 9 8 1 0 1 0 0 0 0 0 0 0 1
Nuclear Periphery 9 8 10 7 10 8 47 54 57 53 81 17 7 4 0 0 6 2 3 0 0 1
Nucleolus 57 11 20 49 84 127 202 202 214 158 171 32 51 61 34 15 2 1 0 2 1 2
Peroxisomes 58 12 35 109 320 199 155 46 33 13 12 46 166 184 2 1 84 15 63 28 127 158
SpindlePole 18 2 16 34 91 50 66 23 21 6 12 10 27 25 1 1 15 2 8 0 16 10
Vac/Vac Membrane 6 1 0 1 8 3 40 9 24 17 36 5 1 1 7 0 1 0 1 1 1 4
Unique Cell Count 230 65 131 260 569 542 953 764 874 642 755 217 322 336 56 17 244 83 150 74 352 402
Labelled Cell Count 354 92 202 380 858 773 1294 1014 1115 856 1003 305 485 495 62 21 244 83 150 74 352 402


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.7 11.4 7.7 7.8 8.5 7.7 8.8 8.2 8.4 8.3 8.8 10.0 10.6 11.2 27.2 32.9 10.7 10.6 10.8
Std Deviation (1e-4) 2.4 3.0 2.1 2.5 2.7 2.7 3.4 3.7 3.1 3.2 3.5 2.8 3.0 3.8 10.1 10.5 3.2 3.4 3.0
Intensity Change (Log2) 0.02 0.14 -0.0 0.18 0.08 0.13 0.09 0.19 0.37 0.46 0.53 1.82 2.09 0.46 0.46 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.7 1.9 3.4 0 0 0 0 2.0 2.2 4.8 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -4.3 -8.5 -4.4 -3.7 0.6 0.3 2.3 1.4 -0.1 -3.9 -7.1 -8.9 -6.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.3 0 0 -1.3 -0.2 -0.5 0.2 1.1 0.1 0 0 0 0
Nucleolus 0.9 -0.1 2.0 1.6 2.7 2.3 2.3 1.9 -0.1 0.2 0.7 6.3 0
Peroxisomes 2.9 6.1 2.2 -3.0 -7.6 -9.7 -10.7 -11.8 -1.2 4.8 5.5 -3.6 0
SpindlePole 0.2 1.1 -1.0 -2.1 -4.8 0 0 0 -2.6 -1.3 -1.6 -2.3 0
Vacuole 0 0 0 0 0 0 0 2.6 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1038 9.1061 7.8436 7.5374 5.8516 7.1666 3.6059 8.5589 6.7539 6.3647 5.297 7.2369 7.5564 12.9348 9.6815 9.2656 8.0874 9.7304
Actin 0.0353 0.0002 0.0028 0.0227 0.0009 0.0006 0.0293 0.0002 0.0055 0.0125 0.0139 0.0003 0.0315 0.01 0.0068 0.0004 0.0003 0.0001
Bud 0.0003 0 0 0.0005 0 0 0.0002 0 0.0001 0.0001 0.0003 0 0.0004 0 0.0005 0 0 0
Bud Neck 0.0003 0.0001 0.0001 0.0062 0.0001 0 0.0006 0.0002 0.0001 0.0002 0.0005 0 0.006 0.0002 0.0002 0 0.0001 0
Bud Periphery 0.0003 0 0.0001 0.0006 0.0001 0 0.0002 0 0.0001 0.0002 0.0004 0 0.0006 0.0001 0.0007 0 0 0
Bud Site 0.0024 0.0001 0.0002 0.0066 0.0002 0.0001 0.0026 0.0001 0.0003 0.0003 0.0044 0 0.0069 0.0001 0.0023 0 0.0002 0
Cell Periphery 0.0002 0.0001 0.0004 0.0004 0 0 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0005 0.0007 0.0001 0 0 0.0001
Cytoplasm 0.0023 0 0.0059 0.0013 0.0004 0.0001 0.0011 0 0.0004 0.0002 0.0007 0 0.001 0 0.0001 0 0 0
Cytoplasmic Foci 0.0685 0.0744 0.0714 0.0847 0.0622 0.0594 0.0674 0.0665 0.0922 0.0423 0.1013 0.0652 0.0521 0.0599 0.0742 0.0531 0.0774 0.0341
Eisosomes 0.0006 0 0.0003 0.0002 0 0 0.0001 0 0 0.0002 0.0001 0 0.0006 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0011 0 0.0084 0.0009 0.0002 0.0002 0.0003 0 0.0004 0.0005 0.0004 0 0.001 0.0001 0.0001 0 0 0
Endosome 0.0247 0.0033 0.0101 0.0296 0.0448 0.0111 0.0191 0.002 0.0134 0.0427 0.024 0.0012 0.0105 0.0035 0.0042 0.0055 0.0035 0.0026
Golgi 0.0201 0.0118 0.0131 0.0244 0.0397 0.0137 0.0214 0.0112 0.0262 0.0265 0.0198 0.0096 0.0194 0.0127 0.0163 0.0233 0.0163 0.0104
Lipid Particles 0.7045 0.853 0.7692 0.7321 0.673 0.8525 0.7538 0.8463 0.7891 0.7445 0.6899 0.859 0.7466 0.8469 0.7953 0.8066 0.837 0.916
Mitochondria 0.0324 0.0063 0.0095 0.0495 0.0906 0.0042 0.0355 0.0158 0.0126 0.0334 0.0548 0.0276 0.0301 0.011 0.0135 0.0373 0.0134 0.0068
None 0.0187 0 0.0054 0.0003 0.0004 0 0.0099 0 0.0002 0.0001 0.0003 0 0.002 0.0001 0.0001 0 0 0
Nuclear Periphery 0.0015 0.0008 0.0017 0.0013 0.0013 0.0006 0.0012 0.0003 0.0003 0.0009 0.0006 0.0001 0.0105 0.0007 0.0007 0.0002 0.0001 0.0017
Nucleolus 0.0254 0.0148 0.0348 0.0035 0.0012 0.0034 0.0162 0.044 0.0052 0.0018 0.0008 0.0008 0.0319 0.0175 0.0135 0.0004 0.0016 0.009
Nucleus 0.0012 0.0003 0.002 0.0003 0.0003 0.0001 0.0008 0.0007 0.0002 0.0002 0.0005 0 0.0048 0.0008 0.0006 0 0 0.0005
Peroxisomes 0.0372 0.0285 0.0567 0.0298 0.0474 0.0504 0.028 0.0086 0.0394 0.074 0.0299 0.0331 0.0198 0.0107 0.0618 0.0696 0.0485 0.012
Punctate Nuclear 0.0181 0.0038 0.0032 0.0019 0.0349 0.0014 0.0047 0.0009 0.0016 0.0009 0.0038 0.0005 0.0159 0.0019 0.0047 0.001 0.0006 0.0021
Vacuole 0.0013 0.0005 0.002 0.001 0.0004 0.0004 0.002 0.0008 0.0078 0.0069 0.0397 0.0002 0.0021 0.0066 0.0007 0.0003 0.0002 0.0005
Vacuole Periphery 0.0035 0.0019 0.0025 0.0023 0.0019 0.0015 0.0052 0.0021 0.0048 0.0116 0.0138 0.0021 0.006 0.0162 0.0036 0.0021 0.0007 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.8108 17.6533 18.0118 24.1729 14.4021 12.3355 20.6381 20.3293 16.9002 22.4414
Translational Efficiency 0.7822 0.6436 0.8752 0.3668 0.8564 1.3165 0.5064 0.8153 0.9119 0.5533

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1770 1205 420 1054 1697 1859 910 1351 3467 3064 1330 2405

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 891.84 990.35 1433.36 1226.34 909.40 1024.48 1211.36 1235.86 900.44 1011.06 1281.47 1231.69
Standard Deviation 156.58 186.47 232.69 285.24 577.66 183.06 247.00 280.09 419.43 185.16 263.61 282.40
Intensity Change Log 2 0.151154 0.684544 0.459502 0.171905 0.413641 0.442528 0.161668 0.554125 0.450958

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.007662 0.007967 0.016995 0.092222 0.005206 0.005258 0.086028 0.064266 0.006460 0.006323 0.064228 0.076518
Bud Neck 0.000409 0.000602 0.021560 0.004475 0.000752 0.000489 0.002782 0.003522 0.000576 0.000533 0.008712 0.003940
Bud Site 0.000192 0.000193 0.015511 0.004074 0.000254 0.000125 0.001064 0.003387 0.000222 0.000152 0.005626 0.003688
Cell Periphery 0.000043 0.000049 0.000820 0.000226 0.000041 0.000028 0.000072 0.000135 0.000042 0.000037 0.000308 0.000175
Cytoplasm 0.000376 0.000642 0.002127 0.001543 0.000391 0.000407 0.001162 0.001511 0.000383 0.000499 0.001466 0.001525
Cytoplasmic Foci 0.040135 0.077864 0.099641 0.116184 0.045249 0.061561 0.089510 0.126311 0.042638 0.067973 0.092709 0.121873
Eisosomes 0.001238 0.000889 0.000752 0.001477 0.000189 0.000985 0.005040 0.001000 0.000725 0.000947 0.003686 0.001209
Endoplasmic Reticulum 0.000362 0.000639 0.048887 0.001146 0.000645 0.000615 0.000638 0.001026 0.000501 0.000624 0.015874 0.001078
Endosome 0.004937 0.007227 0.135953 0.043859 0.009766 0.006558 0.011542 0.048430 0.007301 0.006821 0.050830 0.046427
Golgi 0.093804 0.117988* 0.027053 0.218175* 0.050104 0.104586 0.207673* 0.225899* 0.072414 0.109857 0.150635* 0.222514*
Lipid Particles 0.816441* 0.749746* 0.486778* 0.358526* 0.856683* 0.785798* 0.489822* 0.375319* 0.836138* 0.771620* 0.488860* 0.367960*
Mitochondria 0.010524 0.011262 0.002591 0.070467 0.005882 0.011940 0.024826 0.064762 0.008252 0.011673 0.017804 0.067262
Mitotic Spindle 0.000605 0.002686 0.029893 0.016555 0.002830 0.001912 0.003783 0.013694 0.001694 0.002216 0.012028 0.014948
None 0.000254 0.000407 0.000088 0.000437 0.000109 0.000240 0.000412 0.000395 0.000183 0.000306 0.000310 0.000413
Nuclear Periphery 0.000290 0.000904 0.005261 0.002957 0.000532 0.000943 0.001028 0.003457 0.000408 0.000928 0.002365 0.003238
Nuclear Periphery Foci 0.002166 0.004292 0.047152 0.003564 0.003753 0.003237 0.001150 0.003841 0.002943 0.003652 0.015677 0.003720
Nucleolus 0.000853 0.001110 0.007054 0.000350 0.001120 0.000749 0.000529 0.000324 0.000984 0.000891 0.002589 0.000335
Nucleus 0.000226 0.000248 0.001810 0.000102 0.001376 0.000443 0.000216 0.000107 0.000789 0.000366 0.000719 0.000105
Peroxisomes 0.018396 0.013123 0.015459 0.045963 0.013800 0.012412 0.068747 0.042885 0.016146 0.012692 0.051919 0.044234
Vacuole 0.000811 0.001390 0.031834 0.010682 0.000980 0.001110 0.002689 0.011266 0.000894 0.001220 0.011893 0.011010
Vacuole Periphery 0.000280 0.000772 0.002783 0.007016 0.000338 0.000603 0.001289 0.008462 0.000308 0.000670 0.001761 0.007828

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.20 -2.74 -14.08 -13.96 -11.09 -0.05 -14.16 -13.42 -13.44 3.07 0.16 -13.95 -19.34 -19.36 -2.25
Bud Neck -1.84 -10.11 -12.30 -11.49 8.09 1.82 -6.37 -8.65 -10.42 -1.95 0.62 -11.09 -14.75 -15.45 6.29
Bud Site 0.11 -5.57 -3.87 -3.88 3.92 2.18 -4.83 -5.31 -5.58 -3.88 1.83 -6.02 -6.31 -6.47 1.85
Cell Periphery -0.49 -4.79 -3.34 -3.19 3.47 2.99 -5.63 -11.58 -14.52 -8.23 1.08 -5.09 -5.42 -5.65 2.31
Cytoplasm -1.89 -2.88 -11.35 -5.30 1.06 0.02 -7.18 -8.18 -10.14 -3.28 -1.39 -5.18 -13.39 -10.99 -0.14
Cytoplasmic Foci -13.51 -8.01 -18.26 -8.34 -1.84 -6.37 -11.86 -19.99 -16.00 -7.54 -13.71 -14.66 -27.31 -17.77 -6.87
Eisosomes 1.80 2.25 -0.96 -3.08 -3.38 -6.46 -10.36 -9.83 -0.10 8.51 -1.83 -8.71 -3.83 -2.18 7.31
Endoplasmic Reticulum -3.28 -6.72 -5.62 -3.25 6.61 0.10 0.06 -1.61 -1.34 -2.34 -0.67 -6.50 -4.16 -2.33 6.26
Endosome -2.38 -14.61 -14.16 -12.82 9.80 2.88 -1.30 -14.48 -15.94 -13.41 0.67 -12.97 -20.46 -20.47 1.09
Golgi -4.76 17.96 -17.34 -12.96 -27.73* -14.42 -23.90 -27.52 -17.73 -2.06 -11.97 -14.87 -31.18 -22.03 -10.82
Lipid Particles 8.34 18.73 43.15* 34.36* 6.38 10.21 33.79* 49.70* 41.83* 8.89 12.31 37.69 65.39 54.33* 11.26
Mitochondria -0.33 4.81 -11.29 -11.10 -13.32 -3.57 -6.08 -13.56 -11.69 -7.65 -2.40 -4.21 -17.41 -16.10 -12.76
Mitotic Spindle -3.10 -5.33 -6.21 -5.27 2.19 1.08 -0.95 -4.68 -5.22 -4.32 -0.94 -5.55 -7.76 -7.43 -1.18
None -1.52 6.80 -5.57 -0.24 -13.15 -4.46 -11.08 -7.81 -4.04 0.39 -2.75 -6.01 -9.10 -2.29 -4.07
Nuclear Periphery -5.68 -5.74 -5.07 -3.83 2.28 -2.56 -3.36 -4.84 -4.12 -4.00 -5.15 -6.67 -6.91 -5.56 -1.74
Nuclear Periphery Foci -2.03 -8.15 -1.40 0.66 7.89 0.73 4.34 -0.07 -0.65 -3.21 -1.08 -6.78 -1.04 -0.08 6.27
Nucleolus -0.51 -2.85 1.22 2.38 3.12 0.83 1.10 1.75 1.64 0.61 0.35 -2.06 2.12 2.78 3.13
Nucleus -0.13 -2.57 0.93 1.86 2.81 1.42 1.74 1.93 1.26 0.67 1.28 0.25 2.07 1.59 2.77
Peroxisomes 2.31 1.09 -6.79 -8.30 -7.11 0.71 -9.90 -8.36 -9.03 4.18 2.31 -9.03 -10.60 -12.27 1.72
Vacuole -1.97 -9.24 -7.87 -7.31 5.92 -0.47 -3.90 -8.87 -8.80 -7.15 -1.68 -9.49 -11.88 -11.47 0.59
Vacuole Periphery -3.69 -5.86 -9.52 -8.69 -5.13 -3.29 -5.54 -8.57 -8.28 -7.46 -5.10 -8.11 -12.21 -11.56 -9.47
Standard name
Human Ortholog
Description Protein of unknown function with possible role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion
Localization
Cell Percentages
Cell Cycle Regulation No
Subcompartmental Group N/A

Ldo16

Ldo16


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ldo16-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available