Standard name | |
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Human Ortholog | |
Description | Transcription factor for glycerol biosynthetic genes; required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 |
Bud | 1 | 4 | 3 | 8 | 5 | 5 | 9 | 12 | 21 | 24 | 20 | 13 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 8 | 9 |
Bud Neck | 1 | 2 | 23 | 4 | 17 | 10 | 17 | 16 | 14 | 13 | 10 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 3 | 7 |
Bud Site | 2 | 0 | 2 | 3 | 7 | 3 | 13 | 11 | 29 | 24 | 19 | 15 | 0 | 1 | 2 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 15 | 12 | 19 | 20 | 24 | 36 | 26 | 28 | 32 | 21 | 12 | 26 | 40 | 40 | 3 | 14 | 10 | 2 | 11 | 2 | 0 | 2 | 1 |
Endoplasmic Reticulum | 0 | 2 | 3 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 1 | 4 | 3 |
Endosome | 5 | 4 | 0 | 7 | 10 | 3 | 6 | 1 | 2 | 0 | 5 | 7 | 7 | 65 | 59 | 5 | 10 | 7 | 44 | 44 | 68 | 57 | 67 | 61 |
Golgi | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 3 | 1 |
Mitochondria | 47 | 114 | 18 | 41 | 41 | 135 | 236 | 107 | 359 | 367 | 429 | 212 | 99 | 20 | 35 | 2 | 10 | 12 | 5 | 13 | 24 | 15 | 20 | 15 |
Nucleus | 94 | 107 | 164 | 128 | 130 | 141 | 211 | 218 | 230 | 224 | 242 | 260 | 133 | 114 | 118 | 9 | 39 | 39 | 69 | 91 | 71 | 30 | 33 | 20 |
Nuclear Periphery | 2 | 2 | 3 | 3 | 1 | 1 | 1 | 4 | 7 | 9 | 4 | 6 | 7 | 3 | 2 | 0 | 2 | 2 | 7 | 4 | 15 | 5 | 8 | 9 |
Nucleolus | 19 | 13 | 2 | 8 | 14 | 11 | 25 | 26 | 57 | 50 | 84 | 62 | 45 | 42 | 45 | 6 | 14 | 7 | 33 | 52 | 65 | 28 | 43 | 33 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 3 | 2 |
SpindlePole | 15 | 20 | 93 | 90 | 77 | 72 | 76 | 97 | 66 | 66 | 41 | 77 | 15 | 33 | 24 | 6 | 8 | 11 | 39 | 42 | 88 | 55 | 73 | 62 |
Vac/Vac Membrane | 6 | 14 | 8 | 2 | 5 | 4 | 4 | 3 | 1 | 7 | 7 | 3 | 4 | 15 | 14 | 2 | 6 | 13 | 12 | 35 | 24 | 27 | 29 | 22 |
Unique Cell Count | 160 | 240 | 279 | 240 | 261 | 316 | 456 | 398 | 526 | 568 | 612 | 511 | 272 | 271 | 281 | 26 | 89 | 83 | 229 | 314 | 400 | 244 | 319 | 271 |
Labelled Cell Count | 197 | 298 | 332 | 313 | 329 | 409 | 637 | 523 | 816 | 817 | 884 | 679 | 340 | 336 | 340 | 35 | 104 | 105 | 229 | 314 | 400 | 244 | 319 | 271 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 4.1 | 5.0 | 4.7 | 5.1 | 3.9 | 4.3 | 4.5 | 3.7 | 3.6 | 3.5 | 4.2 | 4.5 | 4.8 | 4.6 | 7.0 | 7.5 | 6.9 | 4.4 | 4.5 | 4.7 |
Std Deviation (1e-4) | 0.6 | 0.7 | 0.6 | 1.3 | 1.2 | 0.9 | 1.1 | 1.1 | 0.7 | 0.6 | 0.7 | 0.9 | 1.0 | 1.3 | 1.0 | 1.6 | 1.4 | 1.5 | 1.7 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.02 | -0.34 | -0.23 | -0.14 | -0.43 | -0.47 | -0.49 | -0.23 | -0.15 | -0.07 | -0.1 | 0.5 | 0.6 | 0.47 | -0.17 | -0.15 | -0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.8853 | 0.2889 | 0.2082 | 0.4156 | 0.1724 | 0.2652 | 0.897 | 0.9082 | 0.921 | 0.5937 | 0.7902 | 0.5637 | -0.0009 | -0.0178 | -0.2722 | 0.8386 | -0.5399 | 0.0749 |
Actin | 0.0199 | 0.0003 | 0.0041 | 0.0376 | 0.0007 | 0.0003 | 0.0132 | 0.0004 | 0.0117 | 0.0034 | 0.0044 | 0.0002 | 0.024 | 0.0004 | 0.0012 | 0.1168 | 0.0011 | 0.0155 |
Bud | 0.0007 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0009 | 0.0002 | 0.0006 | 0.0002 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0003 |
Bud Neck | 0.0049 | 0.001 | 0.0008 | 0.0022 | 0.002 | 0.0029 | 0.0035 | 0.0012 | 0.0035 | 0.0056 | 0.0017 | 0.004 | 0.0033 | 0.0014 | 0.001 | 0.0034 | 0.0011 | 0.0077 |
Bud Periphery | 0.0024 | 0.0004 | 0.0004 | 0.0007 | 0.0001 | 0.0001 | 0.0012 | 0.0002 | 0.0017 | 0.0004 | 0.0007 | 0.0003 | 0.0009 | 0.0005 | 0.0003 | 0.0012 | 0.0003 | 0.0009 |
Bud Site | 0.0082 | 0.0018 | 0.001 | 0.0012 | 0.0004 | 0.0001 | 0.0104 | 0.0012 | 0.0033 | 0.0019 | 0.0016 | 0.0003 | 0.0132 | 0.0009 | 0.0006 | 0.0067 | 0.0003 | 0.0009 |
Cell Periphery | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0004 | 0.0002 | 0.0003 | 0 | 0.0004 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 |
Cytoplasm | 0.0147 | 0.0023 | 0.0019 | 0.001 | 0.0059 | 0.0068 | 0.0029 | 0.0017 | 0.0032 | 0.0005 | 0.0021 | 0.0003 | 0.0074 | 0.0033 | 0.0012 | 0.0009 | 0.0004 | 0.0039 |
Cytoplasmic Foci | 0.0182 | 0.0003 | 0.0017 | 0.0049 | 0.0135 | 0.0002 | 0.014 | 0.0004 | 0.0067 | 0.0096 | 0.0193 | 0.0001 | 0.0256 | 0.0007 | 0.0012 | 0.0017 | 0.0002 | 0.0043 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0007 | 0 | 0 | 0.0014 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0053 | 0.0005 | 0.0058 | 0.001 | 0.0012 | 0.0021 | 0.0021 | 0.0004 | 0.0011 | 0.0003 | 0.0074 | 0.0001 | 0.0022 | 0.0003 | 0.0004 | 0.0008 | 0.0001 | 0.0003 |
Endosome | 0.0256 | 0.0008 | 0.0094 | 0.0036 | 0.0227 | 0.0007 | 0.0235 | 0.0008 | 0.0138 | 0.0187 | 0.0452 | 0.0003 | 0.0182 | 0.0007 | 0.0009 | 0.002 | 0.0004 | 0.0041 |
Golgi | 0.0056 | 0.0003 | 0.004 | 0.0037 | 0.0013 | 0.0001 | 0.0141 | 0.0002 | 0.0048 | 0.0102 | 0.0298 | 0.0001 | 0.0056 | 0.0002 | 0.0004 | 0.0029 | 0.0001 | 0.0094 |
Lipid Particles | 0.0152 | 0.0021 | 0.0145 | 0.0207 | 0.0125 | 0.0009 | 0.037 | 0.001 | 0.0173 | 0.0178 | 0.0648 | 0.0004 | 0.0231 | 0.0005 | 0.0027 | 0.0017 | 0.0001 | 0.0077 |
Mitochondria | 0.01 | 0.0012 | 0.0053 | 0.0021 | 0.0017 | 0.0002 | 0.0071 | 0.0005 | 0.0137 | 0.0047 | 0.0306 | 0.0004 | 0.0027 | 0.0005 | 0.0012 | 0.0042 | 0.0008 | 0.0036 |
None | 0.0034 | 0.0004 | 0.0051 | 0.0009 | 0.0099 | 0.0009 | 0.0022 | 0.0011 | 0.0017 | 0.0004 | 0.0014 | 0.0003 | 0.03 | 0.0144 | 0.003 | 0.0014 | 0.0003 | 0.0071 |
Nuclear Periphery | 0.0501 | 0.032 | 0.0686 | 0.1048 | 0.0553 | 0.038 | 0.0748 | 0.0699 | 0.0916 | 0.1886 | 0.1785 | 0.0272 | 0.0427 | 0.0643 | 0.1126 | 0.1382 | 0.1364 | 0.0308 |
Nucleolus | 0.0818 | 0.0865 | 0.0961 | 0.0671 | 0.21 | 0.1797 | 0.1036 | 0.1242 | 0.1357 | 0.1111 | 0.2326 | 0.2041 | 0.12 | 0.1623 | 0.0939 | 0.1371 | 0.101 | 0.2088 |
Nucleus | 0.5979 | 0.7728 | 0.6171 | 0.5864 | 0.5358 | 0.6851 | 0.5058 | 0.57 | 0.4633 | 0.316 | 0.1904 | 0.6385 | 0.4961 | 0.5361 | 0.5463 | 0.3714 | 0.5642 | 0.5499 |
Peroxisomes | 0.0119 | 0.0007 | 0.0034 | 0.0085 | 0.0016 | 0.0002 | 0.0085 | 0.0001 | 0.0034 | 0.0086 | 0.0099 | 0.0001 | 0.0116 | 0.0001 | 0.0004 | 0.0016 | 0.0001 | 0.0123 |
Punctate Nuclear | 0.1115 | 0.0944 | 0.1565 | 0.1512 | 0.1226 | 0.0808 | 0.1702 | 0.225 | 0.2171 | 0.2985 | 0.1732 | 0.1228 | 0.1686 | 0.2116 | 0.2307 | 0.2039 | 0.1921 | 0.1302 |
Vacuole | 0.0081 | 0.0014 | 0.0017 | 0.0005 | 0.0017 | 0.0004 | 0.0027 | 0.0008 | 0.0029 | 0.0019 | 0.003 | 0.0003 | 0.0019 | 0.0005 | 0.0003 | 0.0006 | 0.0002 | 0.0014 |
Vacuole Periphery | 0.0039 | 0.0006 | 0.002 | 0.0011 | 0.0008 | 0.0003 | 0.002 | 0.0007 | 0.0023 | 0.0015 | 0.0027 | 0.0002 | 0.0012 | 0.0008 | 0.0016 | 0.0012 | 0.0006 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.4938 | 5.4799 | 11.1823 | 11.6341 | 9.9348 | 9.1899 | 14.5443 | 10.4397 | 13.5016 | 12.7374 |
Translational Efficiency | 0.7663 | 1.7047 | 0.7703 | 0.7357 | 0.7581 | 1.0427 | 0.65 | 0.7591 | 0.7066 | 0.8354 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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