Standard name
Human Ortholog
Description P subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0.05 0.06 0 0.08 0.11 0.12
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.12 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.45 0.45 0.51 0.06 0.13 0.06 0.24 0.26 0.2
Mitochondria 0.96 1.0 0.98 0.98 0.96 0.94 0.9 0.87 0.93 0.94 0.96 0.94 0.92 0.96 0.95 0.09 0.12 0.08 0.82 0.67 0.82 0.62 0.52 0.59
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.05 0 0 0 0 0.06 0 0.05 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.23 0.05 0.1 0.07 0.1 0.17 0.18 0.2 0.16 0.17 0.13 0.14 0.1 0.07 0.06 0.16 0.16 0.2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.07 0.17 0.18 0.15 0 0.05 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 6 11 1 4 6 2 0 1 1 7 5 24 12 7 17 7 3 3 12 39 68
Bud 0 0 1 1 6 1 8 8 9 14 0 6 2 3 1 1 1 1 0 0 0 2 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 2 0 1 0 1 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 2 3 0 2 0 1 3 0 3 1 4 5 9 2 5 0 0 0 0 0 2
Cytoplasm 0 0 0 1 0 0 7 5 3 1 1 1 3 2 4 15 16 14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 1 0 2 1 2 4 3 2 0 1 0 0 4 8 6 7 0 0 0 0 1 1
Endosome 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 1 0 0 2 3
Golgi 2 0 0 5 14 0 3 15 1 0 0 1 7 18 26 89 61 115 7 7 5 37 95 116
Mitochondria 135 20 60 216 376 342 624 600 391 502 223 402 223 430 532 17 16 17 114 38 72 97 188 346
Nucleus 0 0 0 0 0 2 1 2 1 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0
Nuclear Periphery 1 1 0 1 9 14 40 30 22 22 10 33 0 0 0 2 0 0 0 0 0 0 0 1
Nucleolus 33 1 6 15 41 62 126 134 68 89 30 60 25 31 31 32 22 45 3 2 0 0 6 7
Peroxisomes 0 0 0 1 4 1 3 3 2 0 2 1 1 4 3 4 0 1 4 0 3 3 5 24
SpindlePole 0 0 0 0 0 0 2 1 1 4 0 0 0 1 1 0 1 0 0 0 0 0 1 0
Vac/Vac Membrane 16 0 2 5 8 12 12 15 10 6 5 10 22 32 37 34 25 34 0 2 0 2 18 5
Unique Cell Count 141 20 61 221 393 362 692 687 422 532 232 428 242 449 561 196 136 225 140 58 88 157 365 585
Labelled Cell Count 188 22 72 253 474 437 836 824 515 644 273 523 291 530 668 223 158 259 140 58 88 157 365 585


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 54.4 36.7 25.3 31.5 31.1 27.4 24.1 23.0 19.4 19.9 17.5 17.2 65.5 60.5 51.7 47.1 51.4 56.3 51.6 50.2 52.5
Std Deviation (1e-4) 13.8 8.5 6.5 7.7 7.9 7.4 6.7 6.5 6.3 5.9 4.9 5.1 18.4 16.2 14.7 18.3 21.5 20.5 13.5 14.8 14.4
Intensity Change (Log2) – – – 0.32 0.3 0.12 -0.07 -0.13 -0.38 -0.34 -0.53 -0.56 1.38 1.26 1.03 0.9 1.03 1.16 1.03 0.99 1.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 6.5 6.3 7.2
Mitochondria -0.3 -1.0 -1.3 -2.1 -2.6 -1.7 -1.3 -0.9 -1.4 -1.7 -1.0 -1.2 -13.4 -11.5 -14.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.1 1.4 1.6 1.9 1.3 1.4 0.7 0.9 0.1 0 0 1.2 1.2 1.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.8 2.8 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.5497 14.25 12.2403 11.7401 10.3705 13.3901 11.3379 21.4697 20.307 13.5605 13.0427 33.4442 7.4109 10.4146 9.8233 9.7616 8.5161 9.5458
Actin 0.0326 0.0002 0.0137 0.0002 0.026 0.0036 0.0197 0.0027 0.0097 0.0229 0.0473 0.0174 0.041 0.0006 0.0003 0.0002 0.0055 0.0001
Bud 0.0017 0.0002 0.0015 0.0002 0.0013 0.002 0.0015 0.0086 0.0062 0.0026 0.0008 0.0031 0.0002 0.0001 0.0001 0.0001 0.0003 0
Bud Neck 0.0107 0.0002 0.0018 0.0004 0.0142 0.0017 0.0027 0.0001 0.0002 0.0003 0.0005 0.0005 0.0006 0.0015 0.0002 0.0003 0.0005 0.0005
Bud Periphery 0.0019 0 0.0007 0 0.0017 0.0009 0.001 0.004 0.0029 0.0021 0.0009 0.0088 0.0002 0 0 0 0.0004 0
Bud Site 0.0067 0.0033 0.0027 0.0001 0.0034 0.0002 0.0177 0.008 0.0028 0.0165 0.0012 0.0005 0.0013 0.0018 0.0001 0 0.0005 0
Cell Periphery 0.0005 0.0002 0.0003 0 0.0004 0.0001 0.0005 0.0004 0.0003 0.0002 0.0001 0.0001 0.0001 0.0005 0 0 0.0001 0
Cytoplasm 0.7577 0.9778 0.9137 0.9806 0.7796 0.9486 0.8012 0.8232 0.7791 0.8626 0.8005 0.4991 0.8581 0.9829 0.996 0.9947 0.8867 0.9961
Cytoplasmic Foci 0.0336 0.0016 0.0089 0.0029 0.0311 0.007 0.0301 0.0146 0.02 0.0289 0.0328 0.0311 0.0105 0.0014 0.001 0.0016 0.0047 0.0007
Eisosomes 0.0003 0 0.0001 0 0.0005 0 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0189 0.0011 0.0025 0.0032 0.0076 0.0041 0.0038 0.0002 0.001 0.0003 0.0019 0.0014 0.0042 0.0038 0.0003 0.0005 0.0031 0.0006
Endosome 0.034 0.0005 0.0113 0.0063 0.0359 0.0041 0.0213 0.0003 0.0111 0.0006 0.0372 0.0218 0.0101 0.0003 0.0003 0.0005 0.0531 0.0003
Golgi 0.0085 0.0001 0.0022 0.0005 0.0162 0.0013 0.0137 0.0002 0.0006 0.0006 0.0421 0.0247 0.0431 0.0001 0 0 0.0189 0
Lipid Particles 0.0116 0 0.0024 0 0.0141 0.0004 0.0276 0.0007 0.0068 0.0019 0.0112 0.0026 0.0026 0.0003 0 0 0.0021 0
Mitochondria 0.0331 0.0101 0.022 0.0001 0.0316 0.0182 0.036 0.123 0.1365 0.0521 0.0068 0.3728 0.0068 0 0 0 0.0088 0
None 0.0082 0.0008 0.0081 0.0004 0.0019 0.0018 0.0041 0.0055 0.0046 0.0024 0.0007 0.001 0.0014 0.0042 0.0006 0.0005 0.0009 0.0004
Nuclear Periphery 0.0087 0.0005 0.0012 0.0009 0.0092 0.001 0.0025 0.0002 0.0009 0.0003 0.0012 0.002 0.0009 0.0005 0.0002 0.0002 0.0057 0.0002
Nucleolus 0.0016 0 0.0004 0 0.0008 0.0001 0.0004 0.002 0.0018 0.0002 0.0001 0.0001 0 0.0001 0 0 0.0002 0
Nucleus 0.0153 0.001 0.002 0.0014 0.0061 0.0021 0.002 0.001 0.0018 0.0012 0.0006 0.0024 0.0011 0.001 0.0006 0.0009 0.0023 0.0011
Peroxisomes 0.0028 0 0.0011 0 0.0064 0.0002 0.0078 0.0024 0.0027 0.0029 0.0113 0.0044 0.0168 0 0 0 0.0003 0
Punctate Nuclear 0.0034 0.0001 0.0006 0.0001 0.0043 0.0002 0.0036 0.0008 0.0008 0.0004 0.0021 0.0051 0.0003 0.0007 0 0.0001 0.0006 0
Vacuole 0.0061 0.0021 0.0024 0.0022 0.0058 0.0017 0.002 0.0012 0.007 0.0005 0.0005 0.0007 0.0003 0.0003 0.0002 0.0002 0.0026 0.0001
Vacuole Periphery 0.002 0.0001 0.0005 0.0004 0.002 0.0007 0.0005 0.0007 0.003 0.0003 0.0004 0.0003 0.0002 0 0 0.0001 0.0027 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 64.6762 25.0962 23.8085 29.3882 20.9639 60.7336 49.2238 49.1025 58.1084 57.9823
Translational Efficiency 0.6564 0.6574 0.5673 0.5474 0.549 0.6849 0.5153 0.6224 0.6361 0.5582

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
329 1088 280 773 673 959 163 1494 1002 2047 443 2267

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2135.81 4318.65 4694.34 3145.92 4523.54 3463.80 2721.76 3304.36 3739.54 3918.16 3968.54 3250.34
Standard Deviation 535.87 1235.85 1261.24 804.37 1080.48 984.49 601.53 884.29 1461.41 1203.26 1429.50 861.16
Intensity Change Log 2 — 1.015797 1.136139 0.558699 — -0.385097 -0.732912 -0.453081 — 0.224843 0.155279 -0.046020

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001892 0.004124 0.005241 0.007164 0.002376 0.006259 0.055984 0.005154 0.002217 0.005124 0.023912 0.005840
Bud Neck 0.001618 0.001719 0.008599 0.002645 0.000845 0.001626 0.005893 0.001298 0.001099 0.001676 0.007604 0.001757
Bud Site 0.002011 0.001498 0.005889 0.005347 0.000755 0.000809 0.002451 0.001005 0.001167 0.001175 0.004624 0.002486
Cell Periphery 0.000125 0.000983 0.000192 0.000483 0.000087 0.000081 0.000500 0.000732 0.000099 0.000560 0.000305 0.000647
Cytoplasm 0.000439 0.000299 0.000490 0.000264 0.000079 0.000514 0.000235 0.000152 0.000197 0.000400 0.000396 0.000190
Cytoplasmic Foci 0.023194 0.029292 0.064270 0.016004 0.020780 0.025580 0.021357 0.011480 0.021572 0.027553 0.048480 0.013023
Eisosomes 0.002644 0.007098 0.002176 0.003201 0.008397 0.004006 0.004756 0.002439 0.006508 0.005650 0.003125 0.002699
Endoplasmic Reticulum 0.000085 0.000131 0.000085 0.000209 0.000027 0.000110 0.001387 0.000166 0.000046 0.000121 0.000564 0.000181
Endosome 0.000510 0.000244 0.001221 0.000937 0.000122 0.000522 0.004372 0.000929 0.000249 0.000374 0.002381 0.000932
Golgi 0.008023 0.008892 0.008902 0.016604 0.005004 0.014707 0.160011* 0.016057 0.005995 0.011616 0.064502 0.016243
Lipid Particles 0.003418 0.005813 0.013393 0.003414 0.003965 0.005974 0.043160 0.006426 0.003785 0.005889 0.024345 0.005399
Mitochondria 0.879730* 0.905334* 0.783947* 0.909422* 0.934410* 0.908395* 0.605097* 0.918259* 0.916456* 0.906768* 0.718140* 0.915246*
Mitotic Spindle 0.000361 0.001080 0.017962 0.001955 0.000768 0.000282 0.002976 0.000750 0.000634 0.000706 0.012448 0.001161
None 0.007571 0.004976 0.010098 0.004022 0.008463 0.005374 0.006741 0.004686 0.008170 0.005163 0.008863 0.004460
Nuclear Periphery 0.000381 0.000363 0.005586 0.000537 0.000350 0.000382 0.004144 0.000614 0.000361 0.000372 0.005056 0.000588
Nuclear Periphery Foci 0.000422 0.000210 0.001131 0.000180 0.000109 0.000239 0.000904 0.000163 0.000211 0.000223 0.001047 0.000169
Nucleolus 0.004536 0.001966 0.004472 0.001735 0.000837 0.002444 0.000670 0.000817 0.002051 0.002190 0.003073 0.001130
Nucleus 0.000299 0.000282 0.000217 0.000153 0.000047 0.000527 0.000125 0.000095 0.000129 0.000397 0.000183 0.000115
Peroxisomes 0.014942 0.015727 0.018561 0.009076 0.005964 0.008082 0.005015 0.009990 0.008912 0.012145 0.013577 0.009678
Vacuole 0.019682 0.004647 0.016352 0.004269 0.002482 0.006485 0.019218 0.004464 0.008129 0.005509 0.017407 0.004398
Vacuole Periphery 0.028119 0.005320 0.031217 0.012379 0.004134 0.007602 0.055003 0.014322 0.012010 0.006389 0.039969 0.013659

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.55 -2.43 -3.99 -2.41 -1.17 -2.86 -5.63 -2.74 0.79 5.33 -3.60 -5.73 -4.49 -0.82 4.74
Bud Neck -0.26 -4.78 -1.80 -1.84 4.00 -1.92 -4.80 -1.20 0.80 4.38 -2.01 -6.59 -2.09 -0.28 5.90
Bud Site 0.52 -2.42 -1.99 -2.44 0.24 -0.15 -1.31 -0.51 -0.64 1.21 -0.05 -3.13 -2.01 -2.18 1.96
Cell Periphery -1.20 -1.69 -2.40 0.68 -1.90 0.07 -3.72 -1.46 -1.48 -0.51 -1.21 -3.60 -1.86 -0.18 -1.15
Cytoplasm 0.66 -0.24 0.89 0.45 1.44 -1.18 -2.14 -2.64 1.07 1.36 -1.16 -1.85 0.08 1.20 2.17
Cytoplasmic Foci -1.26 -4.71 1.44 3.59 5.91 -1.35 -0.39 3.16 5.01 3.25 -2.11 -4.92 3.33 6.67 6.83
Eisosomes -5.34 1.12 -1.03 4.55 -2.22 4.32 2.95 5.97 3.92 2.84 1.09 4.59 5.45 6.16 1.09
Endoplasmic Reticulum -1.03 0.00 -2.42 -1.34 -2.64 -2.29 -4.73 -3.73 -1.11 4.21 -2.74 -4.69 -4.48 -1.55 3.36
Endosome 2.37 -2.45 -2.00 -3.63 0.83 -2.45 -6.43 -3.75 -1.57 4.99 -1.40 -6.90 -4.29 -3.32 4.25
Golgi -0.35 -0.34 -2.43 -2.69 -2.55 -4.93 -8.76 -5.97 -0.55 8.11 -4.13 -7.84 -6.01 -2.66 6.40
Lipid Particles -1.96 -2.92 -0.01 2.33 2.97 -1.75 -4.56 -1.92 -0.32 4.25 -2.52 -5.30 -1.77 0.50 4.84
Mitochondria -1.87 4.70 -2.05 -0.40 -6.93 3.27 12.00 2.28 -1.35 -11.46 1.46 12.49 0.21 -1.49 -12.75
Mitotic Spindle -1.10 -3.30 -1.40 -0.66 2.93 0.90 -1.94 0.02 -2.15 2.12 -0.16 -3.46 -0.97 -0.84 3.30
None 3.32 -2.15 4.55 2.66 6.44 4.08 1.57 5.16 1.86 2.27 5.36 -0.80 6.70 2.73 6.44
Nuclear Periphery 0.22 -2.95 -1.40 -1.52 2.86 -0.40 -3.67 -2.79 -2.40 3.41 -0.20 -3.99 -3.11 -2.92 3.79
Nuclear Periphery Foci 1.92 -3.36 2.29 0.56 5.06 -2.73 -4.04 -2.21 1.64 3.78 -0.27 -5.90 1.09 1.62 6.37
Nucleolus 1.06 0.02 1.11 0.28 2.77 -2.08 0.75 0.09 2.11 -0.72 -0.17 -1.16 1.09 2.25 4.03
Nucleus -0.10 0.71 1.16 0.82 1.11 -1.34 -1.78 -1.32 1.21 0.87 -1.45 -1.42 0.27 1.52 1.99
Peroxisomes -0.19 -0.68 1.45 2.62 2.23 -1.46 0.24 -2.29 -1.04 -1.27 -1.84 -1.51 -0.43 1.55 1.30
Vacuole 4.21 0.73 4.29 0.33 4.07 -3.53 -3.93 -2.05 1.83 3.47 1.86 -3.48 2.71 1.43 5.37
Vacuole Periphery 4.25 -0.45 2.78 -3.30 3.90 -2.52 -6.10 -5.64 -4.09 4.84 2.79 -6.11 -0.74 -5.64 6.10
Standard name
Human Ortholog
Description P subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm
Localization
Cell Percentages mitochondrion (96%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Gcv2

Gcv2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcv2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available