Standard name
Human Ortholog
Description RecQ family nucleolar DNA helicase; role in genome integrity maintenance, chromosome synapsis, meiotic joint molecule/crossover formation; stimulates activity of Top3p; rapidly lost in response to rapamycin in Rrd1p-dependent manner; forms nuclear foci upon DNA replication stress; yeast SGS1 complements mutations in human homolog BLM implicated in Bloom syndrome; also similar to human WRN implicated in Werner syndrome; human BLM and WRN can each complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.55 0.73 0.74 0.78 0.73 0.44 0.53 0.57 0.53 0.54 0.39 0.5 0.63 0.74 0.71 0.3 0.29 0.32 0.41 0.43 0.55 0.54 0.71 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.13 0.15 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.28 0.14 0.37 0.43 0.2 0.63 0.56 0.54 0.64 0.64 0.72 0.6 0.05 0 0.15 0 0.05 0 0.13 0.17 0.18 0.11 0.08 0.12
Nucleus 0.07 0 0 0 0 0 0.06 0 0.06 0 0.06 0.07 0 0 0 0.17 0.2 0.16 0.1 0.06 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.21 0.23 0 0 0.13 0.07 0 0 0 0 0 0 0.32 0.23 0.19 0.54 0.48 0.49 0.18 0.19 0.12 0.2 0.11 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 7 5
Bud 0 0 2 1 2 0 3 3 5 8 3 5 1 0 4 1 0 1 1 4 0 2 6 6
Bud Neck 0 0 0 0 0 0 2 2 1 1 0 2 0 0 0 0 0 0 0 2 0 1 3 1
Bud Site 1 0 0 0 0 1 4 7 1 2 0 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 2
Cytoplasm 41 161 56 49 122 81 153 219 102 161 58 132 199 310 341 109 98 123 48 116 47 116 324 360
Endoplasmic Reticulum 0 0 1 0 0 1 2 2 0 1 0 0 1 5 7 21 19 25 2 1 1 1 2 8
Endosome 3 3 0 0 8 1 0 1 1 0 0 0 12 16 7 18 43 60 4 8 0 4 6 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 1 4 0 0 2 0
Mitochondria 21 31 28 27 33 116 161 208 123 191 106 158 15 9 74 13 15 6 15 46 15 22 34 58
Nucleus 5 4 0 0 6 8 16 16 11 13 9 18 11 9 6 64 68 61 11 15 0 10 3 0
Nuclear Periphery 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 2 1 1 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 1 0 3 3 2 3 2 2 1 4 1 2 1 5 6 6 3 11 1 4 9 6
Vac/Vac Membrane 16 51 1 1 22 13 11 10 1 7 1 11 99 96 92 197 159 189 21 50 10 43 48 27
Unique Cell Count 75 221 76 63 168 184 290 385 192 300 147 263 314 418 479 368 333 389 117 274 86 216 457 493
Labelled Cell Count 90 252 89 78 196 224 355 473 249 388 180 337 340 449 532 429 409 473 117 274 86 216 457 493


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.7 3.4 3.6 4.5 3.2 3.1 3.2 3.0 2.5 2.7 2.4 4.3 4.6 3.8 5.8 5.7 6.6 4.3 4.2 4.7
Std Deviation (1e-4) 0.5 1.1 2.2 2.1 3.8 1.5 1.9 1.8 2.3 1.0 1.2 1.3 1.0 1.2 0.9 1.0 1.2 1.5 1.1 1.1 1.6
Intensity Change (Log2) – – – 0.09 0.41 -0.1 -0.13 -0.11 -0.18 -0.45 -0.34 -0.49 0.34 0.42 0.16 0.77 0.75 0.94 0.33 0.29 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 8.3 7.5 7.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1136 0.3644 0.3054 -0.1981 0.4586 -0.1682 1.9692 1.6333 1.4493 1.6156 0.9947 1.4246 -0.085 0.2609 -0.0983 0.3372 -0.0728 -0.14
Actin 0.047 0.0238 0.0198 0.0006 0.0007 0.0005 0.0143 0.0006 0.0021 0.0029 0.0085 0.0041 0.0068 0.0002 0.011 0.0117 0.0045 0.004
Bud 0.0039 0.0006 0.0005 0.0001 0.0001 0.0001 0.0009 0.0023 0.0006 0.002 0.0002 0.0005 0.0003 0.0002 0.0012 0.0002 0.0003 0.0001
Bud Neck 0.0082 0.0019 0.0085 0.0008 0.0008 0.008 0.0046 0.0006 0.0016 0.003 0.0008 0.0072 0.0038 0.0004 0.0026 0.0244 0.0009 0.0027
Bud Periphery 0.0032 0.0016 0.0011 0.0001 0 0.0001 0.0011 0.0008 0.0007 0.002 0.0002 0.0003 0.0006 0.0001 0.0004 0.0005 0.0003 0.0002
Bud Site 0.044 0.0104 0.0248 0.001 0.0002 0.0005 0.0122 0.011 0.0018 0.0061 0.0005 0.0003 0.0107 0.0013 0.0176 0.0014 0.0014 0.0003
Cell Periphery 0.002 0.0012 0.0015 0.0002 0.0001 0.0001 0.0003 0.0003 0.0001 0.0002 0.0001 0.0001 0.0003 0.0001 0.0003 0.0003 0.0001 0.0001
Cytoplasm 0.1944 0.365 0.3087 0.3709 0.3002 0.3083 0.2402 0.3912 0.3074 0.2651 0.2106 0.2936 0.1666 0.1731 0.1902 0.1666 0.2158 0.2064
Cytoplasmic Foci 0.0235 0.0192 0.0087 0.01 0.0025 0.0121 0.0277 0.016 0.017 0.0152 0.011 0.02 0.0595 0.0069 0.025 0.0286 0.0285 0.0163
Eisosomes 0.0009 0.0009 0.0005 0.0001 0.0001 0.0002 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0142 0.0163 0.0234 0.0087 0.0043 0.0069 0.0124 0.0071 0.0046 0.0051 0.0029 0.0031 0.009 0.0047 0.0053 0.0039 0.0033 0.0036
Endosome 0.0303 0.0054 0.0113 0.0071 0.0022 0.0077 0.0332 0.0086 0.0163 0.0187 0.0134 0.0113 0.031 0.0169 0.01 0.014 0.0131 0.0068
Golgi 0.0105 0.0022 0.0026 0.0002 0.0001 0.0002 0.0129 0.0006 0.0058 0.0107 0.0135 0.0021 0.0144 0.0005 0.003 0.0062 0.0055 0.0016
Lipid Particles 0.0214 0.004 0.0044 0.0008 0.0002 0.002 0.0175 0.0015 0.004 0.0165 0.0106 0.0013 0.0216 0.0012 0.0038 0.0278 0.009 0.0046
Mitochondria 0.0535 0.006 0.0017 0.0006 0.0005 0.0009 0.0267 0.001 0.0041 0.0153 0.0081 0.0023 0.004 0.0006 0.0011 0.0211 0.0292 0.0018
None 0.4239 0.4581 0.4931 0.3879 0.4542 0.3338 0.4063 0.3408 0.3744 0.3045 0.2659 0.2433 0.557 0.5968 0.5735 0.5576 0.4876 0.5231
Nuclear Periphery 0.0195 0.0088 0.0105 0.0101 0.0081 0.0089 0.0327 0.0132 0.0168 0.0245 0.0221 0.012 0.0122 0.0076 0.0121 0.0105 0.0059 0.0119
Nucleolus 0.0053 0.0014 0.0011 0.0029 0.0044 0.0052 0.006 0.002 0.0036 0.0029 0.0029 0.003 0.0098 0.0024 0.0029 0.0011 0.0019 0.0032
Nucleus 0.0542 0.0421 0.0654 0.1804 0.1674 0.2793 0.1089 0.1662 0.2013 0.2449 0.3886 0.3455 0.0374 0.169 0.1 0.0683 0.1619 0.1626
Peroxisomes 0.0096 0.0037 0.0006 0.0002 0.0001 0.0002 0.0114 0.0008 0.0028 0.0059 0.0041 0.0007 0.0194 0.0004 0.0058 0.0433 0.0128 0.0127
Punctate Nuclear 0.0081 0.0212 0.0076 0.0138 0.0531 0.021 0.0219 0.0301 0.0296 0.0485 0.0338 0.0451 0.0324 0.0144 0.0293 0.0102 0.0156 0.0357
Vacuole 0.0177 0.0058 0.0035 0.0034 0.0008 0.0036 0.006 0.0046 0.0043 0.0043 0.0014 0.0034 0.0022 0.0026 0.0043 0.0016 0.0016 0.0017
Vacuole Periphery 0.0047 0.0005 0.0006 0.0003 0.0002 0.0003 0.0027 0.0006 0.001 0.0014 0.0006 0.0006 0.0007 0.0006 0.0004 0.0006 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.7003 14.5322 10.6151 11.5813 13.9966 10.4085 17.8504 14.7208 13.063 14.0039
Translational Efficiency 0.3506 0.4177 0.3202 0.2645 0.2201 0.4231 0.3103 0.2826 0.2574 0.2895

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description RecQ family nucleolar DNA helicase; role in genome integrity maintenance, chromosome synapsis, meiotic joint molecule/crossover formation; stimulates activity of Top3p; rapidly lost in response to rapamycin in Rrd1p-dependent manner; forms nuclear foci upon DNA replication stress; yeast SGS1 complements mutations in human homolog BLM implicated in Bloom syndrome; also similar to human WRN implicated in Werner syndrome; human BLM and WRN can each complement yeast null mutant
Localization
Cell Percentages cytoplasm (17%), nucleus (12%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sgs1

Sgs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sgs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available