Standard name | |
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Human Ortholog | |
Description | Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | – | – | – | 2 | 1 | 0 | 1 | 5 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 4 | 2 | 2 | 8 | 3 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 13 | 1 | 2 | 23 | 17 | 17 | 38 | 15 | 6 | 10 | 6 | – | – | – | 25 | 28 | 18 | 0 | 5 | 0 | 0 | 0 | 2 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | – | – | 16 | 9 | 9 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 0 | 3 | 0 | 0 | 1 | 2 | 0 | 5 | 1 | 0 | 1 | 1 | – | – | – | 14 | 8 | 6 | 1 | 2 | 1 | 0 | 0 | 0 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 2 | 0 | 4 | 0 | 0 | 1 | 0 |
Mitochondria | 49 | 22 | 3 | 14 | 5 | 30 | 45 | 69 | 89 | 75 | 103 | 69 | – | – | – | 20 | 12 | 7 | 6 | 2 | 1 | 0 | 1 | 0 |
Nucleus | 135 | 209 | 17 | 71 | 128 | 147 | 232 | 252 | 169 | 160 | 121 | 162 | – | – | – | 180 | 108 | 148 | 138 | 218 | 51 | 8 | 8 | 16 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 2 | 0 | 3 | 1 | – | – | – | 1 | 1 | 0 | 4 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 2 | 3 | – | – | – | 2 | 0 | 1 | 7 | 5 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 1 | 3 | 4 | 6 | 9 | 2 | 3 | 1 | 0 | – | – | – | 4 | 1 | 2 | 2 | 6 | 1 | 0 | 1 | 0 |
Vac/Vac Membrane | 7 | 32 | 0 | 3 | 11 | 15 | 25 | 9 | 5 | 6 | 9 | 3 | – | – | – | 43 | 34 | 30 | 21 | 30 | 11 | 2 | 1 | 2 |
Unique Cell Count | 166 | 255 | 18 | 80 | 165 | 193 | 294 | 332 | 233 | 204 | 174 | 190 | 261 | 182 | 201 | 188 | 287 | 74 | 13 | 21 | 28 | |||
Labelled Cell Count | 199 | 281 | 21 | 93 | 171 | 218 | 330 | 393 | 287 | 252 | 259 | 248 | 309 | 202 | 224 | 188 | 287 | 74 | 13 | 21 | 28 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 5.0 | 5.0 | 4.9 | 6.6 | 5.0 | 5.0 | 5.3 | 4.5 | 4.4 | 4.2 | 4.3 | – | – | – | 6.6 | 6.6 | 6.6 | – | – | – |
Std Deviation (1e-4) | 0.5 | 1.2 | 0.7 | 1.3 | 2.8 | 1.4 | 1.3 | 1.8 | 1.2 | 0.9 | 1.4 | 0.8 | – | – | – | 1.3 | 1.6 | 1.8 | – | – | – |
Intensity Change (Log2) | – | – | – | -0.02 | 0.42 | 0.01 | 0.01 | 0.1 | -0.15 | -0.19 | -0.23 | -0.2 | – | – | – | 0.42 | 0.41 | 0.4 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleus | -0.7 | -1.7 | -1.8 | -1.6 | -1.8 | -2.0 | -1.6 | -2.2 | -1.1 | – | – | – | -2.3 | -2.9 | -2.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1205 | 0.8859 | 0.8455 | 1.0709 | 0.579 | 0.6035 | 0.787 | 1.05 | 0.7635 | 1.1006 | 2.0759 | 0.8288 | 0.4081 | 0.4293 | 0.206 | 0.5344 | 0.1766 | 0.1305 |
Actin | 0.0098 | 0.0001 | 0.0012 | 0.0043 | 0.0023 | 0.0004 | 0.0121 | 0.0001 | 0.0125 | 0.0331 | 0.0165 | 0.001 | 0.0142 | 0 | 0.0141 | 0.0033 | 0.0415 | 0.001 |
Bud | 0.0006 | 0.0009 | 0.0004 | 0.0024 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0013 | 0.0025 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0003 | 0.0005 | 0.0001 |
Bud Neck | 0.0033 | 0.0001 | 0.0004 | 0.0036 | 0.0002 | 0.001 | 0.0016 | 0.0001 | 0.0011 | 0.0013 | 0.0059 | 0.001 | 0.001 | 0.0001 | 0.0008 | 0.002 | 0.0012 | 0.001 |
Bud Periphery | 0.0014 | 0.0002 | 0.0012 | 0.0048 | 0.0002 | 0.0002 | 0.0009 | 0 | 0.0003 | 0.0018 | 0.0065 | 0.0003 | 0.0013 | 0.0001 | 0.0009 | 0.0015 | 0.0011 | 0.0003 |
Bud Site | 0.0027 | 0.0003 | 0.0011 | 0.0034 | 0.0003 | 0.0002 | 0.0016 | 0.0001 | 0.0018 | 0.0038 | 0.0555 | 0.0001 | 0.0016 | 0.0001 | 0.0012 | 0.0006 | 0.0011 | 0.0005 |
Cell Periphery | 0.0004 | 0 | 0.0003 | 0.0006 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0012 | 0.0036 | 0 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0006 | 0.0001 |
Cytoplasm | 0.0046 | 0.0009 | 0.0015 | 0.0029 | 0.0004 | 0.0003 | 0.0057 | 0.0009 | 0.0017 | 0.0034 | 0.012 | 0.0001 | 0.0074 | 0.007 | 0.0015 | 0.0004 | 0.0075 | 0.0015 |
Cytoplasmic Foci | 0.0145 | 0.0007 | 0.0035 | 0.0081 | 0.0044 | 0.001 | 0.0282 | 0 | 0.0058 | 0.0507 | 0.0443 | 0.0012 | 0.0068 | 0.0001 | 0.0016 | 0.0065 | 0.0116 | 0.0005 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0004 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0008 | 0.0001 |
Endoplasmic Reticulum | 0.005 | 0.0001 | 0.0038 | 0.0013 | 0.0004 | 0.0001 | 0.0025 | 0.0001 | 0.0003 | 0.0029 | 0.0099 | 0.0003 | 0.0029 | 0.0003 | 0.001 | 0.0007 | 0.0059 | 0.0003 |
Endosome | 0.0289 | 0.002 | 0.014 | 0.0299 | 0.0139 | 0.0076 | 0.0265 | 0.0001 | 0.003 | 0.039 | 0.056 | 0.0016 | 0.0145 | 0.0002 | 0.0028 | 0.0031 | 0.0234 | 0.0012 |
Golgi | 0.0075 | 0.0006 | 0.0075 | 0.0166 | 0.006 | 0.0046 | 0.0062 | 0 | 0.005 | 0.0129 | 0.0151 | 0.0011 | 0.0034 | 0 | 0.0013 | 0.0016 | 0.0112 | 0.0008 |
Lipid Particles | 0.0239 | 0.0042 | 0.002 | 0.0115 | 0.0097 | 0.001 | 0.0187 | 0 | 0.0078 | 0.0742 | 0.0157 | 0.0092 | 0.009 | 0 | 0.0021 | 0.007 | 0.0389 | 0.0043 |
Mitochondria | 0.0193 | 0.0011 | 0.0094 | 0.0453 | 0.0401 | 0.0058 | 0.0126 | 0.0012 | 0.0081 | 0.0063 | 0.055 | 0.001 | 0.0055 | 0.0003 | 0.0077 | 0.0041 | 0.0159 | 0.0075 |
None | 0.0052 | 0.0061 | 0.0003 | 0.0006 | 0.0004 | 0.0002 | 0.0053 | 0.0001 | 0.011 | 0.0137 | 0.0052 | 0.0001 | 0.0062 | 0.0028 | 0.005 | 0.0003 | 0.0101 | 0.0011 |
Nuclear Periphery | 0.0386 | 0.0193 | 0.0132 | 0.0124 | 0.0032 | 0.0023 | 0.0108 | 0.0064 | 0.0044 | 0.0043 | 0.0268 | 0.0056 | 0.022 | 0.0076 | 0.0058 | 0.0067 | 0.0194 | 0.0031 |
Nucleolus | 0.0328 | 0.0087 | 0.0134 | 0.0218 | 0.0175 | 0.0293 | 0.0095 | 0.0087 | 0.0052 | 0.0116 | 0.0094 | 0.0148 | 0.0094 | 0.0064 | 0.0076 | 0.0075 | 0.0217 | 0.0144 |
Nucleus | 0.7536 | 0.9343 | 0.8947 | 0.7969 | 0.8887 | 0.9271 | 0.8188 | 0.9809 | 0.9203 | 0.7014 | 0.5368 | 0.9557 | 0.8609 | 0.9721 | 0.9288 | 0.9316 | 0.7162 | 0.9469 |
Peroxisomes | 0.0093 | 0.0004 | 0.0004 | 0.0126 | 0.0068 | 0.0005 | 0.0152 | 0 | 0.0048 | 0.0137 | 0.0229 | 0.0016 | 0.0039 | 0 | 0.0053 | 0.0185 | 0.0058 | 0.0011 |
Punctate Nuclear | 0.0204 | 0.0196 | 0.0229 | 0.0024 | 0.004 | 0.017 | 0.0174 | 0.0009 | 0.0061 | 0.0041 | 0.0881 | 0.0047 | 0.0233 | 0.0022 | 0.0108 | 0.0018 | 0.052 | 0.0139 |
Vacuole | 0.0122 | 0.0003 | 0.0055 | 0.0136 | 0.0005 | 0.0009 | 0.0038 | 0.0002 | 0.0003 | 0.0174 | 0.0076 | 0.0002 | 0.0034 | 0.0005 | 0.0004 | 0.0015 | 0.0079 | 0.0003 |
Vacuole Periphery | 0.0059 | 0.0002 | 0.003 | 0.005 | 0.0008 | 0.0005 | 0.0018 | 0.0002 | 0.0002 | 0.0016 | 0.0042 | 0.0005 | 0.0024 | 0.0002 | 0.0004 | 0.0008 | 0.0058 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.1569 | 7.2323 | 6.6052 | 7.4362 | 9.2792 | 5.5979 | 8.8595 | 7.7829 | 6.2426 | 8.801 |
Translational Efficiency | 1.4499 | 1.4759 | 1.5686 | 1.3967 | 1.2039 | 1.8893 | 1.051 | 1.2149 | 1.5469 | 1.3726 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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