Standard name
Human Ortholog
Description One of several homologs of bacterial chaperone DnaJ; located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.08 0 0.1 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.45 0.75 0.69 0.9 0.88 0.9 0.87 0.87 0.86 0.84 0.91 0.88 0.83 0.94 0.94 0.35 0.34 0.32 0 0 0 0.07 0.05 0.1
Endoplasmic Reticulum 0 0.08 0 0 0.05 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0.08 0.06 0.16 0.07 0.07 0.1
Endosome 0 0 0.17 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.08 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.12 0.11 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.47 0.14 0.3 0.09 0.08 0 0.12 0.12 0.12 0.15 0.06 0.11 0.07 0 0 0.64 0.69 0.69 0.82 0.82 0.67 0.75 0.78 0.68
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 2 4 4 3 2 3 2 2 2 1 1 3 0 0 0 1 0 2 0 0 0
Bud 0 0 0 5 6 10 35 16 26 8 9 6 0 0 0 0 0 0 0 1 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 2 1 0
Bud Site 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 0 1 3 5 1 2 1 2 3 2 3 5 1 4 0 0 0 0 0 0 0 0 0
Cytoplasm 48 27 130 157 194 209 372 338 236 252 136 189 165 124 264 108 64 61 3 2 6 8 10 21
Endoplasmic Reticulum 1 3 2 3 10 7 4 1 1 6 1 3 24 5 5 9 6 3 10 5 32 8 14 21
Endosome 4 1 32 6 2 9 13 16 7 5 1 5 4 0 1 4 6 8 1 1 9 3 7 12
Golgi 1 0 2 0 0 0 0 0 0 0 0 0 0 0 1 3 1 2 0 0 1 0 1 2
Mitochondria 11 3 1 1 0 0 6 3 4 16 7 8 0 0 0 6 7 1 2 1 9 4 4 4
Nucleus 3 0 0 0 0 0 1 2 0 3 0 2 0 1 0 54 23 21 1 0 0 0 1 0
Nuclear Periphery 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 1 0 1 0 1 1 0 1 1 2 2 0 0 0 1 0 0 0 0 2 0 0 0
Peroxisomes 0 0 2 0 0 0 2 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 2 0 1 0 4 2 1 0 1 2 1 1 3 1 0 0 0 1 0 0 0 0
Vac/Vac Membrane 50 5 56 15 18 9 51 48 32 44 9 23 14 4 9 197 130 132 106 71 138 87 153 144
Unique Cell Count 106 36 189 174 220 232 426 388 273 299 150 216 198 132 282 308 188 192 130 87 206 116 198 213
Labelled Cell Count 119 42 229 193 241 250 494 431 314 341 171 246 214 137 292 383 237 228 130 87 206 116 198 213


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.8 6.6 6.8 7.4 6.9 6.4 6.2 6.1 5.6 5.3 5.0 7.5 6.7 6.5 9.6 9.1 9.3 8.0 8.4 8.9
Std Deviation (1e-4) 0.8 0.9 1.8 1.6 1.5 1.6 1.5 1.5 1.7 1.4 1.7 1.2 1.7 1.1 1.8 2.8 2.4 2.5 1.3 1.5 1.6
Intensity Change (Log2) 0.05 0.17 0.06 -0.04 -0.07 -0.11 -0.24 -0.31 -0.39 0.2 0.02 -0.01 0.55 0.46 0.5 0.28 0.35 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 4.1 2.8 4.4 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5.0 4.8 5.5 5.5 5.3 4.6 4.0 4.9 4.6 3.4 5.5 7.1 -7.3 -6.7 -7.2
Endoplasmic Reticulum 0 2.1 0 0 0 0 0 0 0 4.3 0 0 0 0 0
Endosome -4.2 -5.9 -4.5 -6.1 -5.2 -5.5 -6.2 -5.0 -5.1 -5.0 -5.0 -6.9 -6.5 -4.4 -4.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 2.8 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 6.1 5.0 4.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -5.0 -5.6 -7.3 -5.3 -5.1 -4.8 -4.0 -5.5 -4.8 -5.8 -6.0 -8.2 7.4 7.7 7.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4114 5.0921 4.539 4.1792 4.3262 4.5443 2.9333 4.6856 4.3909 3.4347 3.2724 4.2949 2.9959 4.7753 4.0046 4.1767 3.5623 3.9871
Actin 0.0169 0 0.0004 0 0.0002 0.0008 0.0193 0.0001 0.0115 0.013 0.0631 0.0014 0.005 0 0.0023 0.0001 0.0403 0
Bud 0.0006 0.0022 0.0005 0.0005 0.0013 0.0004 0.0008 0.0004 0.001 0.0009 0.0006 0.0006 0.0005 0.0003 0.0004 0.0041 0.0004 0.0004
Bud Neck 0.0015 0.0017 0.0015 0.0009 0.002 0.002 0.0021 0.0022 0.0038 0.0013 0.0014 0.0037 0.0013 0.0015 0.0013 0.0023 0.0014 0.0021
Bud Periphery 0.0012 0.0014 0.0015 0.0012 0.0023 0.002 0.0011 0.0009 0.0018 0.0016 0.0011 0.0022 0.0015 0.0013 0.0011 0.0087 0.001 0.0019
Bud Site 0.002 0.0016 0.0025 0.0008 0.005 0.0011 0.0071 0.0032 0.0125 0.0022 0.0017 0.0014 0.0015 0.0017 0.0013 0.0118 0.0013 0.0007
Cell Periphery 0.0075 0.0113 0.0104 0.0041 0.0045 0.0072 0.0039 0.006 0.0065 0.0024 0.004 0.0054 0.0113 0.0152 0.0072 0.0116 0.0112 0.0142
Cytoplasm 0.0239 0.0109 0.0353 0.0355 0.0123 0.0081 0.0299 0.0493 0.0469 0.0048 0.0021 0.0142 0.0304 0.0246 0.0213 0.0099 0.0046 0.0076
Cytoplasmic Foci 0.0114 0.0099 0.0192 0.0091 0.0117 0.0079 0.0288 0.0071 0.0128 0.0347 0.0115 0.0072 0.0162 0.0074 0.0066 0.0054 0.0115 0.0033
Eisosomes 0.0002 0 0 0 0 0 0.0002 0 0.0001 0.0001 0.0001 0 0 0 0 0 0.0006 0
Endoplasmic Reticulum 0.035 0.0319 0.0489 0.0125 0.0092 0.0253 0.0428 0.0291 0.0342 0.0031 0.008 0.0125 0.0481 0.0689 0.0608 0.0173 0.0049 0.0276
Endosome 0.2095 0.0762 0.1436 0.1947 0.2145 0.1428 0.2534 0.1195 0.2076 0.2092 0.209 0.1065 0.206 0.07 0.1512 0.1299 0.0871 0.0942
Golgi 0.0051 0.0011 0.004 0.0034 0.0058 0.0034 0.0222 0.0021 0.0138 0.0598 0.0877 0.0038 0.0069 0.0018 0.0061 0.0029 0.0108 0.0026
Lipid Particles 0.0101 0.0044 0.0072 0.0096 0.0046 0.0133 0.0224 0.0097 0.013 0.0181 0.0174 0.0107 0.0139 0.0083 0.0147 0.0018 0.0209 0.0107
Mitochondria 0.0023 0.0015 0.0015 0.001 0.0012 0.0024 0.0071 0.0007 0.0059 0.0249 0.0246 0.0008 0.0046 0.0011 0.0015 0.01 0.0272 0.0013
None 0.0156 0.0008 0.0031 0.0004 0.0003 0.0002 0.0046 0.0006 0.0006 0.0003 0.0002 0.0003 0.0023 0.001 0.0004 0.0003 0.0002 0.0001
Nuclear Periphery 0.0261 0.0077 0.0123 0.0046 0.0186 0.0133 0.0516 0.0122 0.0071 0.0036 0.0029 0.0148 0.0124 0.0133 0.0117 0.0055 0.0031 0.0072
Nucleolus 0.003 0.0025 0.0016 0.0007 0.0021 0.0009 0.0023 0.0035 0.0024 0.0021 0.0028 0.0033 0.0022 0.0018 0.0019 0.0021 0.0007 0.0012
Nucleus 0.0169 0.0149 0.0166 0.0022 0.0176 0.0037 0.0105 0.0079 0.0066 0.0027 0.0017 0.0083 0.0048 0.0038 0.0044 0.0051 0.0019 0.0038
Peroxisomes 0.0007 0.0002 0.0005 0.0002 0.0002 0.0002 0.0064 0.0001 0.0015 0.0112 0.0009 0.0074 0.0005 0.0001 0.0022 0.0001 0.0287 0.0002
Punctate Nuclear 0.0022 0.0028 0.0042 0.0001 0.001 0.0003 0.0045 0.0003 0.0011 0.001 0.0003 0.0037 0.0016 0.0002 0.0004 0.0001 0.0015 0.0001
Vacuole 0.5643 0.7858 0.643 0.6543 0.6428 0.7068 0.4303 0.6975 0.5633 0.5462 0.4883 0.7387 0.587 0.7375 0.6551 0.6862 0.6993 0.7787
Vacuole Periphery 0.0442 0.031 0.042 0.0641 0.0428 0.0577 0.0488 0.0477 0.0461 0.0568 0.0708 0.0531 0.0419 0.0401 0.048 0.0846 0.0414 0.0422

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.8129 38.2721 38.0812 36.453 52.3549 32.0919 41.5552 35.9119 50.66 46.1954
Translational Efficiency 1.8848 1.5167 1.2439 1.565 1.3136 1.9273 1.3299 1.4282 1.0648 1.3897

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
183 186 384 1175 2282 1219 524 1105 2465 1405 908 2280

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 902.79 950.80 1066.87 1051.84 807.81 859.23 1068.11 1125.81 814.86 871.35 1067.59 1087.69
Standard Deviation 115.64 132.76 118.75 151.15 104.01 104.81 126.49 164.06 107.83 113.25 123.28 161.82
Intensity Change Log 2 0.074751 0.240922 0.220453 0.089028 0.402972 0.478875 0.081511 0.319720 0.348270

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000189 0.000130 0.005135 0.025551 0.000152 0.000181 0.002424 0.019761 0.000155 0.000174 0.003571 0.022745
Bud Neck 0.017868 0.011491 0.007663 0.020716 0.024163 0.012448 0.012378 0.023484 0.023695 0.012322 0.010384 0.022058
Bud Site 0.012214 0.009335 0.011569 0.063243 0.010373 0.012756 0.013019 0.053287 0.010510 0.012303 0.012406 0.058418
Cell Periphery 0.001875 0.001353 0.003040 0.007558 0.000364 0.001116 0.001782 0.014536 0.000476 0.001148 0.002314 0.010940
Cytoplasm 0.037279 0.056748 0.193154* 0.203787* 0.186739* 0.043043 0.266082* 0.208019* 0.175644* 0.044857 0.235240* 0.205838*
Cytoplasmic Foci 0.069271 0.058158 0.090857 0.064908 0.232253* 0.024806 0.095963 0.042242 0.220154* 0.029221 0.093803 0.053923
Eisosomes 0.000239 0.000042 0.000956 0.002625 0.000194 0.000103 0.000824 0.004129 0.000197 0.000095 0.000880 0.003354
Endoplasmic Reticulum 0.019719 0.010631 0.164901* 0.151936* 0.010692 0.017382 0.069231 0.223026* 0.011362 0.016488 0.109690* 0.186390*
Endosome 0.128335 0.084342 0.029713 0.033689 0.134536 0.058992 0.022416 0.023144 0.134076 0.062347 0.025502 0.028578
Golgi 0.003955 0.001515 0.001878 0.009057 0.003501 0.004588 0.000878 0.012168 0.003534 0.004181 0.001301 0.010565
Lipid Particles 0.015778 0.005847 0.050588 0.077526 0.040703 0.006472 0.032329 0.109605* 0.038852 0.006389 0.040051 0.093073
Mitochondria 0.000518 0.000179 0.000152 0.001848 0.001336 0.003848 0.000182 0.000885 0.001275 0.003362 0.000169 0.001382
Mitotic Spindle 0.001124 0.002505 0.002238 0.005985 0.006247 0.001775 0.000614 0.002703 0.005867 0.001871 0.001301 0.004394
None 0.000050 0.000027 0.000444 0.000132 0.001421 0.000225 0.000501 0.000289 0.001319 0.000199 0.000477 0.000208
Nuclear Periphery 0.000424 0.000333 0.004095 0.000558 0.000613 0.000722 0.002427 0.000268 0.000599 0.000670 0.003132 0.000418
Nuclear Periphery Foci 0.004827 0.004169 0.057884 0.013553 0.009014 0.000756 0.033628 0.015168 0.008703 0.001208 0.043886 0.014336
Nucleolus 0.000727 0.000237 0.000302 0.000207 0.003211 0.000894 0.000423 0.000171 0.003026 0.000807 0.000372 0.000189
Nucleus 0.003992 0.005580 0.059034 0.015326 0.016857 0.010121 0.079031 0.016068 0.015902 0.009520 0.070574 0.015686
Peroxisomes 0.001964 0.003206 0.001650 0.004740 0.004660 0.001915 0.001793 0.003813 0.004460 0.002086 0.001732 0.004291
Vacuole 0.666639* 0.737738* 0.314396* 0.294253* 0.308568* 0.785357* 0.363753* 0.225250* 0.335151* 0.779053* 0.342879* 0.260811*
Vacuole Periphery 0.013015 0.006435 0.000352 0.002805 0.004403 0.012500 0.000324 0.001981 0.005042 0.011697 0.000336 0.002406

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.80 -2.54 -10.86 -10.89 -6.69 -0.77 -4.21 -11.16 -11.14 -9.51 -0.60 -3.92 -15.42 -15.40 -11.31
Bud Neck 1.83 3.26 -0.64 -3.59 -7.36 7.61 6.11 0.49 -5.85 -5.04 7.95 8.81 1.44 -6.95 -7.92
Bud Site 0.82 0.13 -10.36 -13.75 -13.36 -1.39 -1.80 -12.24 -10.60 -10.49 -1.17 -1.83 -18.83 -16.00 -16.75
Cell Periphery 0.98 -1.22 -7.03 -7.19 -4.34 -6.33 -3.75 -11.14 -10.49 -9.70 -5.47 -4.67 -14.44 -13.35 -10.65
Cytoplasm -2.01 -13.06 -19.30 -14.29 -1.29 25.02 -6.48 -2.96 -23.27 4.12 24.14 -6.33 -4.99 -30.41 2.69
Cytoplasmic Foci 0.85 -1.82 0.50 -0.68 4.49 38.44* 21.06 34.53 -6.27 10.48 36.67 22.28 32.51 -9.48 10.28
Eisosomes 1.76 -3.45 -8.33 -10.06 -5.41 1.37 -4.31 -8.94 -9.22 -7.33 1.68 -5.73 -12.23 -12.92 -9.17
Endoplasmic Reticulum 1.73 -10.94 -15.36 -15.61 0.95 -4.46 -9.19 -23.27 -22.31 -14.24 -3.57 -14.61 -28.87 -27.53 -8.82
Endosome 2.75 7.84 7.63 5.07 -1.15 16.79 31.17* 31.00* 11.60 -0.47 16.37 31.66 30.96 11.46 -1.71
Golgi 2.29 2.24 -3.39 -7.84 -8.38 -1.30 13.20 -5.06 -3.84 -6.82 -0.89 10.86 -7.19 -4.94 -10.06
Lipid Particles 2.50 -5.95 -11.13 -16.53 -4.48 15.40 2.82 -12.17 -19.76 -13.24 15.64 0.06 -13.88 -25.95 -12.99
Mitochondria 1.70 1.86 -2.12 -2.92 -2.97 -2.63 11.09 1.94 3.06 -2.87 -2.51 11.50 -0.00 2.39 -3.85
Mitotic Spindle -0.78 -1.61 -3.35 -1.33 -0.48 4.49 6.52 2.23 -1.09 -2.03 4.30 3.35 1.51 -2.45 -1.77
None 0.96 -1.80 -2.77 -4.42 1.42 5.85 4.49 5.71 -0.29 1.37 5.99 4.34 6.46 0.15 2.14
Nuclear Periphery 0.51 -6.75 -1.15 -1.36 6.44 -0.71 -5.68 7.73 3.27 6.86 -0.51 -8.56 2.72 1.86 9.11
Nuclear Periphery Foci 0.17 -9.86 -4.33 -4.41 8.58 8.72 -8.51 -4.31 -15.30 6.62 8.23 -12.26 -4.76 -16.49 10.68
Nucleolus 2.15 1.53 2.32 0.75 1.75 5.48 8.95 9.71 3.03 4.23 5.82 9.07 9.76 2.99 4.05
Nucleus -0.71 -11.77 -10.85 -4.22 9.06 4.19 -13.48 -0.63 -3.88 12.92 4.38 -16.41 -1.50 -5.10 15.79
Peroxisomes -0.79 0.44 -4.76 -0.76 -5.65 5.61 9.61 3.28 -3.11 -5.06 5.05 10.64 1.78 -3.75 -7.55
Vacuole -2.15 12.41 13.55 17.92 0.61 -47.59* -6.25 5.99 50.62* 10.39 -45.56* -3.00 5.32 53.87* 7.74
Vacuole Periphery 2.90 5.89 4.65 3.77 -3.25 -9.83 19.03 5.22 11.68 -3.75 -8.94 18.11 5.72 11.58 -4.70
Standard name
Human Ortholog
Description One of several homologs of bacterial chaperone DnaJ; located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins
Localization
Cell Percentages vacuole (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Scj1

Scj1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Scj1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available