Standard name | |
---|---|
Human Ortholog | |
Description | SR protein kinase (SRPK); involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 2 | 1 | 2 | 1 | 3 | 1 | 5 | 3 | 7 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 13 | 7 | 9 | 12 | 6 | 10 | 12 | 18 | 19 | 25 | 6 | 9 | 8 | 14 | 7 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 179 | 155 | 205 | 249 | 328 | 359 | 377 | 398 | 283 | 339 | 264 | 300 | 310 | 341 | 384 | 117 | 98 | 98 | 169 | 151 | 212 | 152 | 327 | 303 |
Endoplasmic Reticulum | 2 | 1 | 2 | 3 | 3 | 6 | 18 | 12 | 11 | 16 | 24 | 20 | 3 | 0 | 6 | 9 | 7 | 1 | 1 | 1 | 0 | 8 | 11 | 9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 |
Mitochondria | 1 | 1 | 0 | 0 | 0 | 9 | 19 | 7 | 27 | 37 | 60 | 27 | 0 | 1 | 2 | 0 | 2 | 1 | 0 | 1 | 1 | 0 | 1 | 1 |
Nucleus | 1 | 0 | 2 | 2 | 1 | 2 | 3 | 9 | 2 | 5 | 2 | 0 | 0 | 3 | 1 | 8 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 0 | 0 | 2 | 0 | 9 | 1 | 46 | 20 | 37 | 18 | 15 | 1 | 1 | 2 | 0 | 1 | 1 | 2 | 1 | 4 | 2 | 11 | 9 |
Unique Cell Count | 184 | 156 | 209 | 258 | 331 | 380 | 415 | 469 | 336 | 417 | 359 | 360 | 318 | 346 | 395 | 123 | 101 | 101 | 179 | 163 | 221 | 167 | 362 | 334 |
Labelled Cell Count | 187 | 160 | 224 | 264 | 343 | 399 | 428 | 487 | 362 | 457 | 399 | 390 | 326 | 356 | 403 | 148 | 117 | 111 | 179 | 163 | 221 | 167 | 362 | 334 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.2 | 8.5 | 9.5 | 9.2 | 9.6 | 8.1 | 7.9 | 8.3 | 7.6 | 7.6 | 7.1 | 7.8 | 10.3 | 10.4 | 10.2 | 12.8 | 12.6 | 11.9 | 9.4 | 9.4 | 10.2 |
Std Deviation (1e-4) | 1.5 | 1.5 | 1.0 | 1.0 | 1.1 | 1.1 | 1.1 | 1.1 | 1.1 | 1.2 | 1.2 | 1.5 | 1.4 | 1.4 | 1.3 | 2.4 | 2.3 | 2.3 | 1.0 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.02 | -0.22 | -0.27 | -0.19 | -0.32 | -0.31 | -0.42 | -0.28 | 0.13 | 0.13 | 0.11 | 0.44 | 0.41 | 0.33 | -0.01 | -0.01 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.9 | -2.0 | -1.8 | -3.3 | -2.7 | -1.4 | -1.0 | -0.5 | 0.3 | -2.6 | -2.1 | -2.7 | 1.7 | 0.2 | 0.2 |
Cytoplasm | -1.0 | 1.0 | -2.1 | -3.4 | -5.1 | -5.1 | -5.9 | -7.4 | -5.4 | -0.5 | 0.4 | -0.7 | -1.5 | -0.6 | -0.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.3 | 0 | 0 | 2.0 | 3.2 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.1 | 0 | 4.2 | 4.4 | 6.2 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 4.7 | 3.6 | 4.4 | 3.3 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.0575 | 5.3806 | 4.7886 | 4.6864 | 3.97 | 5.0578 | 4.8927 | 6.8395 | 6.4281 | 6.032 | 6.2873 | 6.2994 | 5.9821 | 7.7955 | 7.1407 | 6.6028 | 6.2404 | 6.7728 |
Actin | 0.0418 | 0.0002 | 0.0398 | 0.0192 | 0.0298 | 0.0083 | 0.0187 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0022 | 0.0266 | 0.0012 | 0.0143 | 0.0377 | 0.0155 | 0.003 |
Bud | 0.0007 | 0.0001 | 0.0008 | 0.0003 | 0.0051 | 0.0006 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0034 | 0.0003 | 0.0014 | 0.0006 | 0.0009 | 0.0002 |
Bud Neck | 0.0141 | 0.0002 | 0.0012 | 0.0003 | 0.0025 | 0.001 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0006 | 0.0012 | 0.0001 | 0.0009 | 0.001 | 0.001 | 0.0007 |
Bud Periphery | 0.0009 | 0 | 0.0007 | 0.0003 | 0.0114 | 0.0005 | 0.0006 | 0 | 0 | 0 | 0 | 0.0013 | 0.0014 | 0.0001 | 0.0008 | 0.0006 | 0.0011 | 0.0002 |
Bud Site | 0.0065 | 0.0008 | 0.0064 | 0.0006 | 0.006 | 0.0003 | 0.0023 | 0.0002 | 0.0013 | 0.0001 | 0.0001 | 0.0008 | 0.0009 | 0.0046 | 0.0021 | 0.0216 | 0.0017 | 0.0004 |
Cell Periphery | 0.0006 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 |
Cytoplasm | 0.7211 | 0.9774 | 0.8321 | 0.8658 | 0.765 | 0.8833 | 0.7053 | 0.9888 | 0.9694 | 0.9109 | 0.9229 | 0.9603 | 0.8667 | 0.9776 | 0.9204 | 0.9066 | 0.7975 | 0.9317 |
Cytoplasmic Foci | 0.0389 | 0.002 | 0.0162 | 0.0167 | 0.0418 | 0.0134 | 0.0502 | 0.0021 | 0.0057 | 0.0032 | 0.0074 | 0.0038 | 0.0312 | 0.0032 | 0.0167 | 0.0034 | 0.0108 | 0.0158 |
Eisosomes | 0.0005 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0223 | 0.0117 | 0.0047 | 0.0121 | 0.0109 | 0.0075 | 0.016 | 0.002 | 0.0013 | 0.0054 | 0.0008 | 0.004 | 0.0068 | 0.0014 | 0.0036 | 0.0035 | 0.0008 | 0.0048 |
Endosome | 0.0452 | 0.001 | 0.0424 | 0.0216 | 0.0447 | 0.037 | 0.0723 | 0.0015 | 0.0131 | 0.0292 | 0.0352 | 0.008 | 0.0132 | 0.0009 | 0.0117 | 0.0077 | 0.0318 | 0.0199 |
Golgi | 0.0193 | 0.0001 | 0.0109 | 0.012 | 0.0283 | 0.0181 | 0.0128 | 0.0001 | 0.0045 | 0.0095 | 0.0047 | 0.0024 | 0.0106 | 0.0004 | 0.0089 | 0.0068 | 0.0544 | 0.0095 |
Lipid Particles | 0.0125 | 0 | 0.0062 | 0.0032 | 0.0084 | 0.0039 | 0.0323 | 0.0005 | 0.0003 | 0.0216 | 0.0229 | 0.0006 | 0.0041 | 0.0011 | 0.0081 | 0.0028 | 0.008 | 0.0007 |
Mitochondria | 0.0174 | 0.0001 | 0.0065 | 0.0035 | 0.0116 | 0.003 | 0.0118 | 0 | 0.0002 | 0.0104 | 0.0001 | 0.01 | 0.0083 | 0.0001 | 0.0018 | 0.001 | 0.0685 | 0.0018 |
None | 0.0289 | 0.0036 | 0.0052 | 0.002 | 0.004 | 0.0017 | 0.0091 | 0.0028 | 0.0019 | 0.0013 | 0.0008 | 0.0011 | 0.005 | 0.0014 | 0.0017 | 0.0016 | 0.0005 | 0.0008 |
Nuclear Periphery | 0.0099 | 0.0008 | 0.0037 | 0.0242 | 0.0123 | 0.0034 | 0.0119 | 0.0004 | 0.0003 | 0.0013 | 0.0002 | 0.0007 | 0.0024 | 0.0004 | 0.0008 | 0.0013 | 0.0005 | 0.0014 |
Nucleolus | 0.0008 | 0 | 0.0041 | 0.0001 | 0.0009 | 0.0002 | 0.0009 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Nucleus | 0.0045 | 0.0011 | 0.0064 | 0.0039 | 0.0038 | 0.0124 | 0.0054 | 0.0006 | 0.0006 | 0.0009 | 0.0007 | 0.0009 | 0.0021 | 0.0006 | 0.0008 | 0.001 | 0.0008 | 0.0012 |
Peroxisomes | 0.0047 | 0.0001 | 0.0021 | 0.0097 | 0.0046 | 0.0006 | 0.0196 | 0 | 0.0001 | 0.0008 | 0.0011 | 0.0005 | 0.0136 | 0.0048 | 0.0039 | 0.0007 | 0.0023 | 0.0044 |
Punctate Nuclear | 0.0022 | 0.0002 | 0.0027 | 0.001 | 0.0034 | 0.0006 | 0.0087 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.001 | 0.0015 | 0.0011 | 0.0005 | 0.0002 | 0.0003 |
Vacuole | 0.005 | 0.0005 | 0.0057 | 0.0018 | 0.0031 | 0.0031 | 0.0171 | 0.0004 | 0.0006 | 0.0026 | 0.0017 | 0.001 | 0.0007 | 0.0002 | 0.0004 | 0.0009 | 0.0019 | 0.0023 |
Vacuole Periphery | 0.0022 | 0 | 0.0015 | 0.0017 | 0.0017 | 0.0012 | 0.0031 | 0 | 0.0001 | 0.0018 | 0.0005 | 0.0005 | 0.0004 | 0 | 0.0002 | 0.0003 | 0.0014 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.8194 | 38.0567 | 32.8369 | 38.3138 | 42.36 | 42.0536 | 44.1838 | 45.4489 | 41.4578 | 49.3723 |
Translational Efficiency | 0.7779 | 0.8739 | 0.7403 | 0.69 | 0.6843 | 0.7486 | 0.7811 | 0.6437 | 0.6935 | 0.5723 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|