Standard name
Human Ortholog
Description RNA binding protein involved in synthesis of 18S and 5.8S rRNAs; component of ribosomal small subunit (SSU) processome and 90S preribosome; required for pre-rRNA packaging and compaction of processome into dense terminal balls; part of Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription with role in biogenesis of large ribosomal subunit; binds single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.32 0.26 0.22 0.4 0.23 0.34 0.32 0.25 0.38 0.37 0.3 0.32 0.53 0.4 0.49 0.19 0.07 0.09 0 0 0 0 0 0
Nucleus 0.17 0.31 0.15 0.08 0.17 0.09 0.08 0.08 0.09 0.08 0.1 0.07 0.08 0.05 0.05 0.22 0.1 0.11 0.08 0.16 0.07 0.06 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.85 0.84 0.9 0.91 0.83 0.87 0.85 0.83 0.82 0.79 0.83 0.88 0.9 0.88 0.84 0.78 0.89 0.86 0.86 0.77 0.84 0.86 0.84 0.79
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0.05 0.07 0.07 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 2 1 0 2 4 3 2 2 0 0 0 0 0 0 0 0 1 1 2 3
Bud Neck 0 1 0 1 1 1 1 3 0 1 1 2 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 1 1 8 8 22 17 10 12 18 0 1 6 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 3 3 4 11 4 8 10 4 14 5 2 10 12 21 2 3 3 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Endosome 0 0 1 0 5 5 0 12 1 0 1 3 1 2 3 0 0 0 0 0 3 0 2 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 1 1
Mitochondria 77 40 61 129 96 144 112 144 149 131 110 118 126 96 135 7 8 8 1 0 3 1 2 7
Nucleus 40 48 43 26 69 40 28 48 34 29 36 25 20 13 13 8 11 10 20 26 20 12 16 8
Nuclear Periphery 0 1 0 0 2 2 3 4 5 4 1 6 0 0 0 0 0 0 0 0 2 0 0 1
Nucleolus 203 130 252 295 347 369 303 474 325 277 302 326 213 210 228 29 96 81 213 124 242 167 219 183
Peroxisomes 1 0 0 0 0 1 2 5 1 3 4 4 2 1 3 0 0 1 0 0 0 0 0 0
SpindlePole 3 0 1 1 2 14 13 27 21 26 27 52 3 4 10 1 0 0 4 0 3 0 3 6
Vac/Vac Membrane 3 0 0 1 2 3 2 5 0 1 1 1 2 1 1 1 1 1 3 3 5 7 8 15
Unique Cell Count 239 154 280 325 417 426 355 569 394 352 366 372 236 239 273 37 108 94 248 161 288 194 260 232
Labelled Cell Count 332 223 361 458 538 592 480 756 562 499 502 559 377 341 420 48 121 105 248 161 288 194 260 232


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 43.1 31.7 19.6 20.6 17.0 14.0 11.7 11.9 10.5 9.7 9.5 8.8 32.5 28.5 23.9 53.8 60.8 59.4 41.4 39.3 37.8
Std Deviation (1e-4) 18.3 13.2 7.0 7.1 6.0 4.2 3.4 4.0 4.2 3.2 3.5 2.8 13.9 12.3 10.8 23.7 24.5 20.7 16.0 14.4 12.9
Intensity Change (Log2) – – – 0.07 -0.2 -0.48 -0.75 -0.72 -0.9 -1.01 -1.05 -1.15 0.73 0.54 0.29 1.46 1.63 1.6 1.08 1.0 0.95


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 3.3 3.5 0 3.1 3.7 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.4 0 0 0 0 2.2 0 0 2.3 2.7 3.8 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.7 0.4 3.4 2.7 1.1 4.4 4.2 2.4 2.8 7.4 4.5 6.8 -0.4 -3.3 -2.9
Nucleus -2.8 0.4 -2.4 -3.0 -3.1 -2.7 -2.8 -2.1 -3.6 -2.4 -3.6 -4.1 1.0 -1.3 -1.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.3 -2.5 -1.4 -1.8 -2.6 -2.7 -3.8 -2.7 -0.9 0.1 -0.8 -2.3 -2.1 -0.3 -1.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2.6 2.8 3.4 3.6 4.3 4.3 6.3 0 0 2.8 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 24.9139 34.502 28.3473 33.4989 32.5605 30.9031 27.9554 43.7565 37.2139 34.6519 38.3062 36.5388 39.6179 43.4955 38.6507 38.4092 38.3846 39.9536
Actin 0.0212 0 0.0151 0 0.0512 0.0001 0.0114 0 0.0001 0.0004 0 0.0026 0.0014 0 0 0 0 0
Bud 0.0003 0 0.0002 0.0001 0.0001 0.0001 0.0004 0 0 0.0001 0 0.0001 0 0 0 0 0 0
Bud Neck 0.0011 0 0.0003 0.0001 0.002 0.0004 0.0006 0 0 0 0 0.0001 0.0001 0 0.0001 0 0 0
Bud Periphery 0.0005 0 0.0005 0.0001 0.0002 0.0003 0.0007 0 0 0.0002 0 0.0003 0.0001 0 0 0 0 0
Bud Site 0.0011 0 0.0009 0.0001 0.0002 0.0002 0.0015 0 0.0001 0.0002 0 0.0001 0.0002 0.0001 0.0001 0 0 0
Cell Periphery 0.0005 0.0002 0.0004 0.0002 0.0003 0.0009 0.0004 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0098 0 0.0003 0 0.0001 0.0003 0.0005 0 0.0002 0.0001 0 0.0001 0.0001 0 0 0 0 0
Cytoplasmic Foci 0.0086 0 0.0054 0.0002 0.0012 0.0005 0.0142 0 0.0051 0.002 0 0.0046 0.0192 0.0001 0.001 0.0005 0 0
Eisosomes 0.0003 0 0.0001 0 0.0001 0.0001 0.0003 0 0 0.0002 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0028 0 0.0016 0 0.0001 0 0.0007 0 0 0.0001 0 0.0002 0 0 0 0 0 0
Endosome 0.0132 0 0.0106 0 0.0025 0.0001 0.0119 0 0.0009 0.0014 0 0.0021 0.0093 0 0 0 0 0
Golgi 0.0038 0 0.0052 0 0.0114 0 0.0083 0 0.0004 0.0018 0 0.0036 0.016 0 0.0001 0 0 0
Lipid Particles 0.0066 0.0001 0.0117 0.0001 0.0008 0.0017 0.0383 0.0001 0.0077 0.0369 0.0003 0.0081 0.017 0 0.0028 0.0011 0 0.0002
Mitochondria 0.0098 0.0005 0.0244 0.0015 0.0117 0.0483 0.0252 0.0002 0.0018 0.0114 0.002 0.0085 0.0019 0.0006 0.0011 0.0007 0.0006 0.0007
None 0.0171 0 0.0002 0 0 0.0003 0.0018 0 0.0006 0.0005 0 0.0002 0.0001 0 0 0 0 0
Nuclear Periphery 0.0283 0 0.0162 0.0001 0.0001 0.0005 0.0073 0 0.0007 0.0029 0 0.0006 0 0 0 0 0 0
Nucleolus 0.7861 0.861 0.8336 0.8206 0.8646 0.8608 0.7913 0.9073 0.8989 0.8662 0.8946 0.8978 0.8352 0.8919 0.8955 0.9326 0.9121 0.9237
Nucleus 0.0587 0.1372 0.0583 0.1762 0.0515 0.0805 0.067 0.0918 0.0789 0.0668 0.1022 0.0654 0.0725 0.1052 0.098 0.0643 0.0869 0.0748
Peroxisomes 0.0022 0 0.0012 0.0001 0.0008 0.0004 0.0087 0 0.0004 0.0037 0 0.0006 0.026 0 0.0006 0.0002 0 0
Punctate Nuclear 0.0217 0.0001 0.0076 0.0003 0.0001 0.0002 0.0047 0.0002 0.0029 0.0008 0.0001 0.0042 0.0003 0.0005 0.0002 0.0002 0 0.0002
Vacuole 0.0026 0.0003 0.0027 0.0003 0.0004 0.0016 0.0019 0.0001 0.0003 0.0006 0.0001 0.0002 0.0002 0.0004 0.0002 0.0001 0.0001 0.0001
Vacuole Periphery 0.0036 0.0004 0.0037 0.0002 0.0005 0.0027 0.0031 0.0001 0.0008 0.0035 0.0005 0.0005 0.0002 0.0006 0.0002 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 191.2355 201.0763 187.7078 150.1579 144.8529 147.2516 140.1346 117.8944 98.0306 130.9564
Translational Efficiency 1.1677 1.2142 1.014 1.03 1.0222 1.0669 0.9704 0.9131 0.892 0.9681

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description RNA binding protein involved in synthesis of 18S and 5.8S rRNAs; component of ribosomal small subunit (SSU) processome and 90S preribosome; required for pre-rRNA packaging and compaction of processome into dense terminal balls; part of Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription with role in biogenesis of large ribosomal subunit; binds single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress
Localization
Cell Percentages nucleolus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Rrp5

Rrp5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrp5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available