Standard name
Human Ortholog
Description Cell fusion regulator; cytoplasmic protein localized to shmoo tip; required for alignment of parental nuclei before nuclear fusion during mating; contains a Dbl-homology domain; binds specifically with activated Cdc42p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.98 0.96 0.94 0.94 0.9 0.71 0.83 0.62 0.71 0.99 0.99 1.0 0.93 0.95 – 0.85 0.79 0.62 0.82 0.83 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.08 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.06 0.06 0.31 0.17 0.55 0.24 0 0 0 0.05 0 – 0.05 0.11 0.24 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 – 4 1 1 1 2 2
Bud 0 0 0 0 0 0 1 1 2 2 3 2 0 0 0 0 0 – 3 0 1 2 5 4
Bud Neck 1 0 0 1 4 1 6 11 5 5 0 1 1 0 0 0 0 – 2 1 1 1 2 3
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 1 2 2 0 0 1 1 1 1 2 0 0 1 1 0 1 0 – 1 1 1 0 4 1
Cytoplasm 314 169 91 61 133 108 187 180 105 176 55 65 205 237 277 57 69 – 270 149 69 108 197 250
Endoplasmic Reticulum 1 0 0 0 0 0 0 3 2 0 0 5 0 1 0 2 6 – 1 0 0 0 1 0
Endosome 1 0 1 0 2 2 1 0 0 4 0 1 1 1 0 2 0 – 1 0 0 1 3 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – 1 1 0 2 0 1
Mitochondria 6 5 0 0 1 8 12 12 45 37 49 22 0 0 0 3 3 – 14 20 27 2 4 4
Nucleus 0 1 1 0 2 1 0 1 0 1 0 2 1 0 0 0 0 – 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 4 6 5 2 2 2 0 1 1 0 0 – 4 0 0 0 4 3
Vac/Vac Membrane 1 0 1 0 1 1 0 0 1 7 0 1 1 1 0 1 0 – 7 4 3 1 3 3
Unique Cell Count 319 173 93 62 138 115 199 201 147 213 89 91 207 240 278 61 73 317 189 113 131 238 286
Labelled Cell Count 325 177 96 64 144 123 214 215 168 238 109 102 210 242 279 68 78 317 189 113 131 238 286


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.8 4.7 5.0 5.5 4.2 4.4 4.5 3.5 4.3 3.3 4.0 5.5 5.8 5.3 5.2 5.3 4.8 5.9 5.9 5.6
Std Deviation (1e-4) 0.7 0.9 0.8 1.3 2.7 1.7 2.0 1.5 0.9 2.2 1.0 2.2 2.4 2.8 2.5 1.4 1.7 1.4 3.8 3.7 3.1
Intensity Change (Log2) – – – 0.09 0.21 -0.18 -0.11 -0.08 -0.45 -0.15 -0.53 -0.26 0.22 0.28 0.17 0.15 0.16 0.02 0.31 0.32 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4319 -0.8273 -0.9837 -0.6485 -2.0796 -1.1089 0.6323 -0.5442 -0.4962 -0.3107 -0.2039 -0.1939 -0.1981 -1.0769 -0.6622 -0.4552 0.3513 -1.0464
Actin 0.0509 0.0003 0.0011 0.001 0 0.0004 0.0785 0.0001 0.0107 0.0021 0.0486 0.0043 0.0969 0.0008 0.0406 0.0568 0.1801 0.0004
Bud 0.014 0.0009 0.0004 0.0001 0 0.0005 0.0234 0.002 0.014 0.0074 0.0003 0.0114 0.0029 0.0024 0.0009 0.0001 0.0073 0.0001
Bud Neck 0.0213 0.0002 0.0007 0.0006 0 0.0003 0.0163 0.0001 0.0037 0.0022 0.0012 0.011 0.0312 0.0003 0.0113 0.0006 0.0022 0.0051
Bud Periphery 0.038 0.0008 0.0005 0.0001 0 0.0014 0.0194 0.0026 0.0094 0.0031 0.0004 0.0085 0.0087 0.0045 0.0018 0.0001 0.0276 0.0001
Bud Site 0.0573 0.0225 0.0306 0.0016 0 0.0002 0.0412 0.0027 0.0144 0.0044 0.0007 0.0005 0.0099 0.0031 0.0091 0.0004 0.0046 0.0001
Cell Periphery 0.0028 0.0003 0.0007 0 0 0.0003 0.001 0.0001 0.0003 0.0005 0.0001 0.0002 0.0013 0.0002 0.0007 0.0001 0.0004 0
Cytoplasm 0.0141 0.0188 0.0212 0.006 0.01 0.0495 0.0207 0.0244 0.0125 0.0109 0.0071 0.0275 0.0255 0.0583 0.0239 0.116 0.0135 0.0996
Cytoplasmic Foci 0.0133 0.0031 0.002 0.1002 0.0012 0.0019 0.0451 0.002 0.0043 0.0382 0.0316 0.0175 0.0475 0.0091 0.0079 0.0236 0.0145 0.0113
Eisosomes 0.0058 0.0002 0.0002 0.0001 0.0001 0 0.0008 0.0001 0.0003 0.0002 0.0003 0.0001 0.0007 0.0001 0.0006 0.0003 0.0009 0.0001
Endoplasmic Reticulum 0.0044 0.0007 0.0006 0.0002 0 0.0009 0.0055 0.0004 0.0004 0.0005 0.0019 0.0006 0.0046 0.0005 0.0017 0.0005 0.0067 0.0007
Endosome 0.0636 0.0185 0.0214 0.0231 0 0.0222 0.0509 0.0004 0.0056 0.0213 0.0194 0.0093 0.0345 0.0022 0.0081 0.0024 0.0266 0.0115
Golgi 0.0067 0.0004 0.0047 0.0181 0 0.0027 0.0151 0.0001 0.0012 0.0035 0.0245 0.0018 0.0128 0.0011 0.0053 0.0053 0.0266 0.0031
Lipid Particles 0.0286 0.0012 0.0013 0.0047 0 0.0003 0.038 0.0002 0.002 0.0222 0.0151 0.0067 0.021 0.0067 0.0059 0.0151 0.027 0.0017
Mitochondria 0.0095 0.0004 0.0018 0.0008 0 0.0162 0.0151 0.0004 0.002 0.0509 0.004 0.015 0.0057 0.0033 0.0149 0.0008 0.0384 0.0036
None 0.5438 0.9211 0.9075 0.8257 0.9854 0.8818 0.5157 0.9603 0.9039 0.7568 0.8243 0.8348 0.6347 0.8962 0.833 0.7489 0.5212 0.8543
Nuclear Periphery 0.0087 0.0004 0.0008 0.0001 0.0001 0.0004 0.0079 0.0002 0.0004 0.0014 0.0024 0.0019 0.012 0.0004 0.0036 0.0003 0.0292 0.0029
Nucleolus 0.0226 0.0003 0.0002 0.0001 0.0002 0.0001 0.0123 0.0006 0.0013 0.0105 0.0003 0.0078 0.0023 0.0022 0.0014 0.0001 0.0105 0.0003
Nucleus 0.0192 0.0003 0.0003 0.0001 0.0003 0.0004 0.0131 0.0008 0.0017 0.0184 0.0004 0.0073 0.0028 0.0014 0.0017 0.0005 0.0177 0.0012
Peroxisomes 0.0045 0.0001 0.0001 0.0163 0 0.0001 0.0368 0.0001 0.0081 0.0222 0.0145 0.0201 0.0254 0.0019 0.0162 0.0267 0.0341 0.0003
Punctate Nuclear 0.0235 0.0003 0.0003 0.0009 0.0025 0.0001 0.0363 0.0016 0.0031 0.0166 0.002 0.0072 0.0149 0.0035 0.0026 0.0012 0.0035 0.0026
Vacuole 0.0387 0.0076 0.0021 0.0002 0 0.0136 0.0051 0.0007 0.0004 0.0055 0.0004 0.0044 0.0036 0.0014 0.0071 0.0002 0.0027 0.0007
Vacuole Periphery 0.0089 0.0015 0.0015 0.0001 0 0.0068 0.0018 0.0001 0.0001 0.0012 0.0005 0.0019 0.0011 0.0004 0.0016 0.0001 0.0047 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.2385 1.4548 4.3182 4.3863 4.9234 13.0123 5.5162 5.6259 5.3551 5.9701
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2073 1600 154 1286 1686 1805 240 121 3759 3405 394 1407

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 646.72 805.44 1044.97 988.45 697.48 736.43 998.97 1073.12 669.49 768.86 1016.95 995.73
Standard Deviation 69.18 121.82 123.39 149.02 80.95 103.80 113.85 175.56 78.84 117.78 119.80 153.34
Intensity Change Log 2 — 0.316636 0.692248 0.612027 — 0.078397 0.518290 0.621588 — 0.197933 0.604605 0.616996

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000047 0.000390 0.001656 0.001222 0.000086 0.000293 0.000609 0.001445 0.000064 0.000339 0.001018 0.001241
Bud Neck 0.007718 0.013967 0.005891 0.013276 0.008054 0.025434 0.002993 0.005220 0.007868 0.020045 0.004126 0.012583
Bud Site 0.004327 0.007704 0.004669 0.042037 0.002568 0.010775 0.008321 0.021948 0.003538 0.009332 0.006894 0.040310
Cell Periphery 0.000289 0.000295 0.000297 0.000372 0.000198 0.000369 0.000205 0.000222 0.000248 0.000334 0.000241 0.000359
Cytoplasm 0.467658* 0.396225* 0.120975* 0.435677* 0.438159* 0.392815* 0.348516* 0.333591* 0.454427* 0.394418* 0.259579* 0.426898*
Cytoplasmic Foci 0.078834 0.073016 0.005539 0.017727 0.110150 0.082208 0.008827 0.024945 0.092880 0.077889 0.007542 0.018347
Eisosomes 0.000057 0.000051 0.000090 0.000035 0.000060 0.000062 0.000072 0.000056 0.000059 0.000057 0.000079 0.000037
Endoplasmic Reticulum 0.000449 0.001993 0.015509 0.004516 0.000810 0.001418 0.006673 0.002812 0.000611 0.001688 0.010127 0.004369
Endosome 0.002348 0.005205 0.003054 0.013482 0.005185 0.007721 0.004394 0.079821 0.003621 0.006539 0.003870 0.019187
Golgi 0.000306 0.001211 0.000178 0.012766 0.000687 0.002235 0.000123 0.006623 0.000477 0.001754 0.000144 0.012238
Lipid Particles 0.003413 0.003073 0.002334 0.000951 0.004992 0.002972 0.001955 0.004029 0.004121 0.003019 0.002103 0.001216
Mitochondria 0.002212 0.002622 0.000918 0.003484 0.004246 0.005955 0.000616 0.004898 0.003124 0.004389 0.000734 0.003606
Mitotic Spindle 0.001481 0.007077 0.001502 0.054385 0.002097 0.009772 0.002241 0.115818* 0.001757 0.008506 0.001952 0.059668
None 0.029914 0.016062 0.003113 0.010883 0.014969 0.016452 0.007650 0.006687 0.023210 0.016269 0.005877 0.010522
Nuclear Periphery 0.000411 0.000695 0.002187 0.002256 0.000623 0.000736 0.001768 0.001622 0.000506 0.000717 0.001932 0.002202
Nuclear Periphery Foci 0.000238 0.000945 0.003949 0.004098 0.000390 0.000449 0.002789 0.002904 0.000306 0.000682 0.003242 0.003996
Nucleolus 0.004037 0.005257 0.001250 0.001043 0.002929 0.006211 0.000678 0.000243 0.003540 0.005763 0.000902 0.000974
Nucleus 0.383455* 0.432495* 0.750441* 0.276582* 0.381615* 0.401466* 0.470463* 0.151590* 0.382630* 0.416047* 0.579896* 0.265833*
Peroxisomes 0.001143 0.003450 0.000364 0.000781 0.002773 0.004468 0.000411 0.002079 0.001874 0.003989 0.000392 0.000893
Vacuole 0.011051 0.027616 0.075834 0.097213 0.018867 0.026273 0.130419 0.222229* 0.014556 0.026904 0.109084 0.107964
Vacuole Periphery 0.000613 0.000652 0.000251 0.007214 0.000544 0.001915 0.000276 0.011217 0.000582 0.001322 0.000266 0.007558

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.73 -8.52 -8.08 -4.01 5.10 -6.87 -9.15 -5.69 -4.42 -1.16 -11.26 -11.30 -8.73 -5.38 3.63
Bud Neck -5.28 -0.05 -3.61 2.04 -3.22 -10.51 4.02 1.41 11.09 -2.22 -11.72 3.35 -3.46 7.50 -5.71
Bud Site -3.26 -1.31 -6.68 -5.04 -2.65 -9.39 -2.25 -4.02 -0.15 -1.82 -8.27 -2.23 -7.57 -4.46 -4.42
Cell Periphery 0.03 -0.93 1.89 2.21 2.90 -4.02 -1.00 -1.01 2.76 -0.21 -2.84 -0.62 0.93 3.47 1.49
Cytoplasm 8.07 29.60 16.38 7.24 -16.24 4.60 10.20 8.35 6.32 0.77 9.17 21.16 17.74 9.56 -8.14
Cytoplasmic Foci 1.37 24.19 23.60 18.18 -2.98 5.26 24.46 24.09 19.40 0.28 4.43 33.97 33.47 26.63 -1.48
Eisosomes 1.37 -6.42 2.04 0.30 7.18 -0.22 -4.90 -2.15 -1.94 2.40 0.57 -7.88 2.36 1.52 8.81
Endoplasmic Reticulum -12.31 -12.40 -12.43 -7.36 8.94 -4.84 -9.80 -5.89 -5.00 3.58 -12.19 -15.28 -12.96 -9.22 9.10
Endosome -5.84 -2.07 -2.29 2.30 -0.52 -2.54 1.06 -0.88 -0.36 -1.09 -5.42 -0.58 -0.83 3.10 -0.40
Golgi -2.79 1.88 -2.84 -1.63 -2.93 -3.21 5.22 -0.26 2.32 -1.65 -4.31 5.85 -2.65 -0.91 -3.07
Lipid Particles 0.59 1.79 6.13 5.19 5.19 2.53 3.66 4.32 1.62 1.33 2.34 4.01 8.20 5.35 6.42
Mitochondria -0.45 2.11 0.65 1.28 -3.51 -1.39 3.68 2.50 5.17 -2.23 -1.74 4.36 2.30 5.05 -5.21
Mitotic Spindle -3.99 -0.24 -9.99 -8.11 -10.01 -4.57 -0.20 -3.45 -2.83 -3.44 -6.10 -0.45 -10.57 -8.39 -10.53
None 7.04 16.26 12.05 5.14 -9.55 -0.86 5.69 1.78 2.43 -2.17 5.64 14.81 11.97 7.49 -4.95
Nuclear Periphery -7.39 -12.65 -18.94 -16.66 -2.09 -1.60 -11.48 -7.49 -6.81 -1.60 -4.78 -17.09 -19.62 -16.94 -3.49
Nuclear Periphery Foci -4.31 -3.14 -7.59 -3.62 1.28 -1.16 -6.55 -6.04 -5.43 2.23 -4.39 -6.38 -8.05 -5.64 2.43
Nucleolus -2.01 6.24 7.73 9.90 1.28 -6.45 6.90 10.57 11.85 2.51 -5.74 9.02 10.30 14.39 1.08
Nucleus -5.18 -16.71 -2.53 2.43 14.87 -2.00 -5.99 2.52 3.34 5.85 -4.92 -13.44 -2.00 1.95 11.24
Peroxisomes -3.91 3.80 4.41 5.72 1.97 -2.30 4.86 4.78 8.27 -0.15 -4.67 6.02 6.34 10.03 1.02
Vacuole -9.42 -8.33 -25.49 -21.75 -3.39 -4.24 -11.73 -11.08 -10.80 -4.63 -10.16 -14.30 -26.85 -24.42 -1.91
Vacuole Periphery -0.18 2.11 -1.98 -2.01 -2.80 -3.04 4.36 -0.86 0.72 -1.26 -2.88 3.56 -2.21 -0.51 -3.03
Standard name
Human Ortholog
Description Cell fusion regulator; cytoplasmic protein localized to shmoo tip; required for alignment of parental nuclei before nuclear fusion during mating; contains a Dbl-homology domain; binds specifically with activated Cdc42p
Localization
Cell Percentages cytoplasm (31%), nucleus (23%), mixed (35%)
Cell Cycle Regulation Yes
nucleus - S/G2 (0.00069)
Subcompartmental Group N/A

Fus2

Fus2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fus2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available