Standard name
Human Ortholog
Description Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I.
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.93 0.97 0.94 0.96 0.96 0.96 0.9 0.89 0.82 0.79 0.72 0.79 0.99 0.98 1.0 0.96 0.92 – 0.69 0.79 0.61 0.84 0.81 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0 0.09 0.17 0.15 0.38 0.27 0.45 0.32 0 0 0 0 0 – 0.16 0.1 0.16 0.08 0.07 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.05 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 2 2 1 1 3 5
Bud 0 2 0 0 0 0 3 1 4 8 3 1 0 1 0 0 0 – 1 0 3 1 4 5
Bud Neck 1 1 4 3 3 5 3 11 4 14 3 2 5 0 1 0 1 – 0 1 1 0 1 2
Bud Site 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 1 2 2 1 1 0 0 0 0 0 0 0 3 2 – 1 1 1 0 1 2
Cytoplasm 191 202 103 115 192 191 250 193 141 190 123 148 211 200 246 297 193 – 135 168 67 109 168 203
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 22 14 – 2 0 0 0 0 3
Endosome 0 1 0 1 1 0 0 1 0 0 0 0 0 1 0 1 4 – 2 2 1 0 0 1
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 – 1 0 0 0 0 1
Mitochondria 24 3 4 4 3 17 47 32 65 65 76 61 1 0 0 7 4 – 30 21 17 10 14 12
Nucleus 0 0 0 0 2 0 4 2 2 3 0 1 0 0 0 0 1 – 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 3 0 0 0 1 0 0 0 0 0 0 – 1 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 1 0 2 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 1 6 6 0 4 2 4 1 0 0 1 1 – 3 0 6 0 3 3
Vac/Vac Membrane 1 4 0 0 1 1 5 6 1 0 0 4 0 2 1 11 9 – 3 10 4 1 2 3
Unique Cell Count 205 208 109 120 199 199 278 217 172 241 170 188 213 204 247 310 209 196 214 110 131 208 251
Labelled Cell Count 219 213 113 126 206 217 323 253 218 284 213 221 218 204 248 343 230 196 214 110 131 208 251


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 3.9 4.9 4.5 4.7 4.0 3.5 4.2 3.1 3.2 3.0 3.4 4.1 4.9 4.7 5.0 5.2 4.7 4.4 4.6 4.8
Std Deviation (1e-4) 0.7 0.9 2.2 2.4 2.2 2.1 1.8 2.3 1.8 1.3 1.4 1.3 0.8 1.8 1.5 1.0 1.3 0.9 1.6 1.4 1.6
Intensity Change (Log2) – – – -0.11 -0.06 -0.29 -0.49 -0.23 -0.65 -0.59 -0.71 -0.54 -0.24 0.01 -0.05 0.05 0.09 -0.05 -0.15 -0.07 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0669 -0.2448 -0.0448 0.5134 0.0834 0.0446 -0.5227 -0.6373 -0.5227 -0.8523 -0.3491 -0.6725 -1.4738 -1.5833 -1.6525 -0.1578 -1.2101 -1.7068
Actin 0.0133 0.0025 0.0022 0.0534 0.0034 0.0004 0.0157 0.0002 0.0214 0.0001 0.064 0.0002 0.0107 0.0003 0.0015 0.0087 0.0117 0.0038
Bud 0.0008 0.0001 0.0003 0.0002 0.0014 0.0001 0.0008 0.0023 0.0014 0 0.0003 0.002 0.002 0.001 0.0013 0.0053 0.0035 0.0002
Bud Neck 0.0041 0.0002 0.0002 0.0007 0.0001 0.0008 0.003 0.0001 0.0027 0.0003 0.0014 0.0005 0.0038 0.0003 0.0001 0.0007 0.0005 0.0019
Bud Periphery 0.0026 0.0001 0.0003 0.0003 0.005 0.0002 0.0021 0.006 0.0026 0 0.0005 0.0072 0.0026 0.002 0.0034 0.0196 0.0145 0.0003
Bud Site 0.0064 0.0005 0.0027 0.0018 0.0022 0 0.0092 0.002 0.0037 0 0.0013 0.0002 0.0197 0.001 0.0024 0.0034 0.0005 0.0001
Cell Periphery 0.0007 0.0001 0.0001 0.0001 0.0002 0 0.0027 0.0001 0.0006 0 0.0001 0.0001 0.0005 0.0001 0.0002 0.0006 0.0002 0.0001
Cytoplasm 0.0666 0.0714 0.0483 0.0626 0.0162 0.07 0.03 0.0265 0.0414 0.0294 0.0208 0.0595 0.0371 0.0585 0.0756 0.0564 0.1432 0.1554
Cytoplasmic Foci 0.0254 0.0062 0.0182 0.0245 0.0231 0.0022 0.016 0.0039 0.0074 0.0008 0.0222 0.0109 0.0365 0.0072 0.0104 0.0283 0.0258 0.0135
Eisosomes 0.0004 0.0005 0.0001 0.0004 0.0002 0.0001 0.0005 0.0001 0.0009 0.0001 0.0004 0.0001 0.0008 0.0003 0.0004 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0058 0.0008 0.001 0.0009 0.0009 0.0006 0.0039 0.0004 0.0027 0.0003 0.0015 0.0003 0.003 0.0009 0.0027 0.0048 0.0026 0.0033
Endosome 0.0282 0.0009 0.018 0.0233 0.014 0.0007 0.0105 0.0004 0.0093 0.0004 0.0244 0.0006 0.0183 0.0011 0.0157 0.104 0.0146 0.0088
Golgi 0.007 0.0001 0.0083 0.0236 0.0057 0.0001 0.0025 0.0001 0.0031 0 0.0078 0 0.0042 0.0001 0.0046 0.0129 0.0213 0.0023
Lipid Particles 0.0145 0.0003 0.0057 0.0276 0.0139 0.0002 0.0043 0.0003 0.0028 0 0.0181 0.0001 0.0221 0.0004 0.0055 0.0069 0.0249 0.0042
Mitochondria 0.0088 0.0004 0.0019 0.0054 0.0097 0.0072 0.0051 0.0024 0.0092 0.0004 0.0028 0.0003 0.0055 0.0009 0.0044 0.0899 0.0135 0.0153
None 0.7418 0.8544 0.8745 0.6897 0.7876 0.877 0.8562 0.9348 0.8639 0.8889 0.7535 0.8826 0.7833 0.887 0.8425 0.4672 0.5421 0.7435
Nuclear Periphery 0.0095 0.0037 0.0007 0.0015 0.0015 0.001 0.0058 0.0008 0.0033 0.0019 0.0136 0.0005 0.0069 0.0006 0.0025 0.0066 0.0133 0.0028
Nucleolus 0.0041 0.0008 0.0002 0.0007 0.0023 0.0013 0.0012 0.0017 0.0008 0.0025 0.0012 0.0007 0.0026 0.0014 0.0005 0.0054 0.0008 0.0003
Nucleus 0.0227 0.0403 0.0085 0.0372 0.054 0.0321 0.0044 0.0057 0.0091 0.0676 0.0031 0.0309 0.0191 0.0293 0.0131 0.1294 0.098 0.0345
Peroxisomes 0.0221 0.0019 0.0057 0.0397 0.0529 0.0004 0.0046 0.0027 0.0079 0 0.0469 0.0001 0.0049 0.0006 0.0077 0.0014 0.0619 0.0028
Punctate Nuclear 0.0078 0.0144 0.0018 0.0033 0.0023 0.005 0.0173 0.0087 0.0039 0.007 0.0141 0.0027 0.0104 0.0062 0.0016 0.0124 0.0018 0.0044
Vacuole 0.005 0.0004 0.0009 0.0025 0.0027 0.0004 0.0032 0.0006 0.0015 0.0002 0.0009 0.0005 0.0044 0.0008 0.0031 0.0274 0.0023 0.002
Vacuole Periphery 0.0022 0.0001 0.0003 0.0006 0.0009 0.0001 0.001 0.0002 0.0005 0 0.0011 0.0001 0.0017 0.0001 0.0008 0.0082 0.003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0118 13.1894 16.7778 12.5926 8.8815 9.4796 10.7162 13.1796 10.4034 11.9342
Translational Efficiency 1.1916 0.8155 0.5503 0.7168 0.9694 1.4927 0.8963 0.6247 0.8214 0.948

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2617 155 1851 1733 1447 2289 1935 1294 4064 2444 3786 3027

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 706.75 756.16 650.33 768.92 629.80 711.83 820.79 807.65 679.35 714.64 737.45 785.48
Standard Deviation 74.55 94.38 132.22 92.67 73.80 85.24 90.46 120.58 82.92 86.53 141.38 107.23
Intensity Change Log 2 — 0.097492 -0.120028 0.121634 — 0.176639 0.382119 0.358836 — 0.135328 0.138401 0.238275

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000152 0.000629 0.000282 0.000569 0.000127 0.000384 0.000117 0.003827 0.000143 0.000399 0.000198 0.001962
Bud Neck 0.025481 0.042593 0.004926 0.007468 0.014445 0.049299 0.004391 0.009105 0.021552 0.048874 0.004653 0.008167
Bud Site 0.008107 0.020221 0.011013 0.026119 0.004146 0.021107 0.006177 0.033788 0.006697 0.021051 0.008542 0.029398
Cell Periphery 0.000160 0.000146 0.000196 0.000205 0.000126 0.000183 0.000134 0.000193 0.000148 0.000180 0.000165 0.000200
Cytoplasm 0.476111* 0.297404* 0.423916* 0.440670* 0.488097* 0.394824* 0.357159* 0.430017* 0.480379* 0.388646* 0.389797* 0.436116*
Cytoplasmic Foci 0.220991* 0.347094* 0.010982 0.022183 0.222542* 0.222858* 0.008124 0.039416 0.221543* 0.230737* 0.009521 0.029550
Eisosomes 0.000192 0.000185 0.000024 0.000056 0.000133 0.000150 0.000027 0.000058 0.000171 0.000152 0.000026 0.000057
Endoplasmic Reticulum 0.000953 0.000843 0.001392 0.002610 0.000722 0.000566 0.001572 0.001500 0.000871 0.000584 0.001484 0.002135
Endosome 0.010630 0.038785 0.001277 0.003607 0.008946 0.023349 0.001105 0.021843 0.010031 0.024328 0.001189 0.011403
Golgi 0.003014 0.007391 0.000102 0.002755 0.001793 0.005696 0.000041 0.020528 0.002579 0.005803 0.000071 0.010352
Lipid Particles 0.009205 0.005878 0.000724 0.001641 0.008735 0.007199 0.000840 0.001382 0.009037 0.007115 0.000783 0.001530
Mitochondria 0.006074 0.007070 0.000643 0.004612 0.007119 0.015060 0.000608 0.012304 0.006446 0.014553 0.000625 0.007900
Mitotic Spindle 0.001438 0.003071 0.002239 0.016808 0.001126 0.011146 0.002665 0.049499 0.001327 0.010633 0.002457 0.030783
None 0.003197 0.002033 0.020212 0.002637 0.003293 0.003344 0.005110 0.007778 0.003231 0.003261 0.012494 0.004835
Nuclear Periphery 0.000365 0.000605 0.000919 0.001836 0.000498 0.000500 0.001612 0.001577 0.000413 0.000507 0.001273 0.001725
Nuclear Periphery Foci 0.000221 0.000553 0.000602 0.001028 0.000302 0.000283 0.000408 0.001003 0.000250 0.000300 0.000503 0.001017
Nucleolus 0.001219 0.002757 0.001118 0.000370 0.001957 0.001986 0.000506 0.000589 0.001482 0.002035 0.000805 0.000464
Nucleus 0.182254* 0.100920 0.499060* 0.363572* 0.194897* 0.156934* 0.579831* 0.240718* 0.186756* 0.153382* 0.540341* 0.311053*
Peroxisomes 0.006176 0.038521 0.000483 0.002696 0.005710 0.016152 0.000148 0.002850 0.006010 0.017571 0.000312 0.002762
Vacuole 0.043097 0.081368 0.019637 0.097489 0.034247 0.066798 0.029257 0.114867 0.039946 0.067722 0.024554 0.104918
Vacuole Periphery 0.000963 0.001933 0.000253 0.001069 0.001038 0.002181 0.000167 0.007158 0.000990 0.002165 0.000209 0.003672

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.74 -1.40 -4.58 1.12 -1.18 -3.26 -0.79 -1.82 -1.50 -1.79 -3.55 -1.50 -2.23 -1.49 -2.01
Bud Neck -2.46 13.38 12.89 5.48 -2.17 -13.14 6.63 4.52 17.40 -2.49 -11.40 15.30 13.22 18.89 -3.37
Bud Site -2.05 -1.14 -3.69 0.54 -2.29 -9.04 -0.96 -6.85 0.66 -5.92 -7.78 -0.88 -6.76 1.48 -5.58
Cell Periphery 0.52 -1.02 0.27 -0.35 1.24 -3.96 0.34 -0.55 3.44 -0.72 -2.17 -0.29 0.25 2.44 0.51
Cytoplasm 8.63 8.84 13.60 -3.30 3.46 9.98 18.04 11.47 2.98 -4.73 13.08 19.49 17.77 3.04 -1.08
Cytoplasmic Foci -6.08 49.62 47.57 16.90 -5.83 0.20 38.49 34.26 40.18 -9.27 -1.42 63.03 58.26 45.24 -11.09
Eisosomes 0.20 20.07 15.43 4.59 -14.92 -1.65 15.37 10.51 11.06 -11.70 2.12 24.15 18.27 12.22 -18.39
Endoplasmic Reticulum -0.40 -4.88 -10.45 -8.20 -7.49 1.55 -12.53 -9.79 -13.12 2.22 2.73 -10.63 -13.00 -17.13 -5.42
Endosome -4.74 13.40 10.66 6.14 -4.20 -9.99 10.53 -0.32 7.87 -6.58 -10.83 16.91 5.89 14.06 -7.53
Golgi -1.96 12.38 2.92 2.75 -2.05 -6.60 6.54 -3.52 -1.21 -4.64 -5.92 13.99 -1.79 1.84 -5.06
Lipid Particles 3.58 16.69 14.76 6.15 -8.64 1.91 10.31 9.77 12.53 -3.06 3.27 19.31 17.65 12.93 -8.00
Mitochondria -0.34 8.33 3.87 2.15 -2.70 -4.89 5.14 1.10 6.15 -3.63 -7.08 9.47 3.15 9.39 -4.53
Mitotic Spindle -1.01 -1.52 -5.67 -3.92 -5.06 -6.45 -2.15 -8.84 -5.52 -8.15 -6.40 -2.48 -10.41 -4.33 -9.48
None 1.91 -10.42 1.84 -1.00 11.15 -0.04 -2.05 -2.25 -2.16 -1.21 0.01 -10.36 -1.17 -0.98 8.91
Nuclear Periphery -2.24 -11.09 -25.68 -10.10 -13.96 -0.11 -20.18 -9.97 -10.29 -1.02 -2.57 -24.25 -22.95 -21.20 -8.96
Nuclear Periphery Foci -2.85 -5.87 -7.98 -2.76 -3.64 0.32 -3.39 -5.32 -5.20 -3.21 -1.27 -6.93 -9.65 -7.97 -5.00
Nucleolus -2.45 1.01 5.11 3.87 6.43 -0.01 3.37 3.24 7.33 -0.76 -2.10 3.77 5.46 8.57 4.65
Nucleus 5.98 -40.30 -27.56 -20.65 13.26 5.77 -48.55 -11.68 -16.62 29.73 6.59 -64.03 -28.35 -30.53 29.37
Peroxisomes -4.71 13.52 6.45 5.43 -1.54 -9.78 10.87 7.47 15.38 -3.01 -11.34 17.91 9.54 15.66 -2.61
Vacuole -4.52 7.92 -24.03 -7.40 -26.57 -11.80 -6.60 -24.71 -19.50 -21.18 -11.70 -0.49 -34.22 -24.97 -32.91
Vacuole Periphery -1.92 6.22 2.02 2.68 -1.77 -4.53 4.54 -2.93 -1.09 -4.62 -6.37 7.84 -1.87 2.05 -4.96
Standard name
Human Ortholog
Description Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I.
Localization
Cell Percentages cytoplasm (25%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rnh1

Rnh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rnh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available