Standard name | |
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Human Ortholog | |
Description | GTPase activating protein (GAP) for Gsp1p; involved in nuclear transport |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 6 | 11 | 11 | 13 | 8 | 11 | 10 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 3 | 0 | 4 | 6 | 10 | 12 | 18 | 13 | 18 | 17 | 1 | 13 | 14 | 9 | 9 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 291 | 127 | 113 | 87 | 109 | 138 | 141 | 131 | 123 | 129 | 96 | 89 | 341 | 368 | 417 | 214 | 196 | 174 | 288 | 126 | 104 | 226 | 273 | 287 |
Endoplasmic Reticulum | 0 | 1 | 4 | 2 | 7 | 15 | 22 | 42 | 45 | 26 | 18 | 32 | 4 | 5 | 4 | 15 | 8 | 6 | 1 | 0 | 8 | 3 | 3 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 3 | 3 | 1 | 1 | 0 | 0 | 0 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 0 | 1 | 7 | 30 | 17 | 16 | 41 | 27 | 40 | 1 | 0 | 0 | 0 | 5 | 3 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 0 | 0 | 0 | 1 | 8 | 1 | 0 | 5 | 2 | 0 | 0 | 2 | 1 | 6 | 16 | 4 | 0 | 1 | 0 | 2 | 3 | 3 |
Unique Cell Count | 294 | 128 | 116 | 88 | 115 | 161 | 200 | 192 | 195 | 195 | 146 | 156 | 344 | 374 | 422 | 228 | 215 | 180 | 297 | 131 | 116 | 243 | 287 | 302 |
Labelled Cell Count | 297 | 129 | 120 | 89 | 121 | 176 | 227 | 217 | 219 | 228 | 174 | 189 | 347 | 391 | 436 | 250 | 239 | 197 | 297 | 131 | 116 | 243 | 287 | 302 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 30.7 | 43.8 | 30.2 | 28.1 | 25.6 | 23.8 | 22.4 | 21.5 | 20.3 | 19.5 | 18.3 | 18.7 | 34.0 | 32.3 | 30.9 | 29.5 | 27.7 | 29.6 | 34.6 | 35.0 | 37.6 |
Std Deviation (1e-4) | 5.6 | 7.8 | 5.9 | 5.1 | 4.9 | 5.0 | 4.7 | 4.8 | 4.1 | 4.7 | 4.4 | 4.3 | 7.2 | 6.5 | 6.1 | 7.8 | 7.3 | 7.8 | 8.6 | 8.9 | 8.8 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.24 | -0.35 | -0.43 | -0.49 | -0.58 | -0.63 | -0.72 | -0.69 | 0.17 | 0.1 | 0.03 | -0.03 | -0.13 | -0.03 | 0.2 | 0.21 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 1.4 | 2.3 | 1.6 | 2.9 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | -1.0 | -3.3 | -5.8 | -6.1 | -6.8 | -6.4 | -6.3 | -7.5 | 1.4 | 0.7 | 1.1 | -1.4 | -2.2 | -0.4 |
Endoplasmic Reticulum | 0 | 0.9 | 1.9 | 2.4 | 4.4 | 4.6 | 2.9 | 2.6 | 4.1 | 0 | 0 | 0 | 1.2 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.4 | 3.3 | 3.2 | 5.3 | 4.9 | 5.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 20.9032 | 28.1895 | 24.1652 | 22.1171 | 19.4096 | 24.7561 | 21.194 | 34.247 | 30.9507 | 26.2007 | 31.6798 | 30.5076 | 25.1576 | 32.3269 | 29.5503 | 28.8285 | 25.6797 | 29.319 |
Actin | 0.0094 | 0.0008 | 0.0055 | 0.0012 | 0.0013 | 0.0011 | 0.0235 | 0.0002 | 0.0012 | 0.0409 | 0 | 0.0014 | 0.0167 | 0.0007 | 0.0004 | 0.001 | 0.0316 | 0.0008 |
Bud | 0.0007 | 0.0004 | 0.0007 | 0.0017 | 0.0007 | 0.0007 | 0.0012 | 0.0002 | 0.0006 | 0.0015 | 0.0001 | 0.0009 | 0.0006 | 0.0002 | 0.0003 | 0.0008 | 0.0014 | 0.0002 |
Bud Neck | 0.0005 | 0.0004 | 0.0018 | 0.0009 | 0.0007 | 0.0014 | 0.0009 | 0.0002 | 0.0005 | 0.0005 | 0.0004 | 0.0012 | 0.0013 | 0.0003 | 0.0004 | 0.0007 | 0.0008 | 0.0011 |
Bud Periphery | 0.0012 | 0.0001 | 0.0006 | 0.0011 | 0.0004 | 0.0008 | 0.0012 | 0.0001 | 0.0002 | 0.0007 | 0 | 0.0006 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.0011 | 0.0001 |
Bud Site | 0.0008 | 0.0004 | 0.0007 | 0.0004 | 0.0004 | 0.0001 | 0.0037 | 0.0002 | 0.0004 | 0.0027 | 0 | 0.0001 | 0.0062 | 0.0005 | 0.0002 | 0.0003 | 0.0021 | 0 |
Cell Periphery | 0.003 | 0.0003 | 0.0017 | 0.0007 | 0.0002 | 0.0006 | 0.0004 | 0.0003 | 0.001 | 0.0002 | 0 | 0.0006 | 0.0016 | 0.0005 | 0.0003 | 0.0003 | 0.0006 | 0.0002 |
Cytoplasm | 0.8112 | 0.9537 | 0.8834 | 0.8239 | 0.7599 | 0.8557 | 0.7453 | 0.9745 | 0.9379 | 0.7802 | 0.9708 | 0.9125 | 0.8313 | 0.9637 | 0.9372 | 0.8763 | 0.7912 | 0.8798 |
Cytoplasmic Foci | 0.016 | 0.0053 | 0.0092 | 0.0081 | 0.0323 | 0.0099 | 0.0251 | 0.0015 | 0.0052 | 0.0302 | 0.0018 | 0.0081 | 0.0087 | 0.003 | 0.0053 | 0.0078 | 0.0153 | 0.0056 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0.0008 | 0 | 0 | 0 | 0.0006 | 0 |
Endoplasmic Reticulum | 0.0438 | 0.0199 | 0.0313 | 0.0087 | 0.0231 | 0.051 | 0.0265 | 0.0096 | 0.0085 | 0.0115 | 0.0034 | 0.023 | 0.0524 | 0.0116 | 0.0224 | 0.0114 | 0.0122 | 0.0627 |
Endosome | 0.0385 | 0.0096 | 0.0244 | 0.0371 | 0.0849 | 0.0401 | 0.0421 | 0.0024 | 0.0136 | 0.0396 | 0.0058 | 0.0284 | 0.0139 | 0.0032 | 0.0106 | 0.0289 | 0.0451 | 0.0193 |
Golgi | 0.0116 | 0.0024 | 0.0042 | 0.0034 | 0.0067 | 0.0079 | 0.0188 | 0.0002 | 0.0029 | 0.0167 | 0.0001 | 0.0029 | 0.0034 | 0.0017 | 0.0008 | 0.0008 | 0.0128 | 0.0032 |
Lipid Particles | 0.0056 | 0 | 0.0005 | 0.0002 | 0.0273 | 0.0003 | 0.015 | 0.0001 | 0.0004 | 0.0043 | 0 | 0.0001 | 0.0063 | 0.0009 | 0.0003 | 0.0002 | 0.0098 | 0.0001 |
Mitochondria | 0.0096 | 0.0005 | 0.004 | 0.0024 | 0.004 | 0.0127 | 0.0124 | 0.0001 | 0.0049 | 0.0156 | 0.0001 | 0.0031 | 0.0087 | 0.003 | 0.0003 | 0.0003 | 0.0119 | 0.0025 |
None | 0.0016 | 0.0002 | 0.0004 | 0.0003 | 0.0016 | 0.0005 | 0.005 | 0.0001 | 0.0002 | 0.0037 | 0.0001 | 0.0004 | 0.0014 | 0.0001 | 0.0003 | 0.0003 | 0.0036 | 0.0006 |
Nuclear Periphery | 0.0042 | 0.001 | 0.0014 | 0.0031 | 0.0232 | 0.0043 | 0.0288 | 0.0012 | 0.0012 | 0.0096 | 0.0014 | 0.0027 | 0.0131 | 0.0008 | 0.0026 | 0.0049 | 0.0241 | 0.0075 |
Nucleolus | 0.0003 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0007 | 0 | 0 | 0.002 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0009 | 0 |
Nucleus | 0.0022 | 0.0007 | 0.0009 | 0.0017 | 0.0031 | 0.001 | 0.0167 | 0.0011 | 0.001 | 0.0196 | 0.001 | 0.0014 | 0.0027 | 0.0006 | 0.001 | 0.002 | 0.0084 | 0.0015 |
Peroxisomes | 0.0085 | 0 | 0.0065 | 0.0001 | 0.0125 | 0.0001 | 0.0037 | 0 | 0.0001 | 0.0044 | 0 | 0.0001 | 0.0012 | 0.002 | 0 | 0.0001 | 0.0011 | 0 |
Punctate Nuclear | 0.0042 | 0 | 0.0001 | 0.0001 | 0.0012 | 0.0002 | 0.0134 | 0 | 0.0001 | 0.005 | 0 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0001 | 0.0036 | 0 |
Vacuole | 0.0226 | 0.0036 | 0.0189 | 0.0951 | 0.0116 | 0.0081 | 0.0126 | 0.0068 | 0.0181 | 0.008 | 0.0118 | 0.0086 | 0.0242 | 0.0064 | 0.0148 | 0.0507 | 0.0151 | 0.0092 |
Vacuole Periphery | 0.0043 | 0.0007 | 0.0037 | 0.0098 | 0.0048 | 0.0033 | 0.0031 | 0.001 | 0.002 | 0.0028 | 0.0031 | 0.0039 | 0.0034 | 0.0007 | 0.0025 | 0.0128 | 0.0067 | 0.0054 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 174.4353 | 149.0846 | 181.1895 | 150.3165 | 141.4249 | 143.2551 | 127.5173 | 131.7106 | 163.196 | 130.9841 |
Translational Efficiency | 0.9466 | 1.068 | 0.8881 | 1.3002 | 1.0411 | 1.1239 | 1.1696 | 0.9982 | 0.8766 | 1.0366 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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