Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 – 0.05 0 0.07 0 0 0 0 0 0.09 0.1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Cytoplasm 0.73 0.77 0.88 0.64 0.61 0.46 – 0.4 0.31 0.32 0.28 0.28 0.84 0.91 0.96 0.23 0.38 0.25 0.3 0.15 0.36 0.33 0.28 0.34
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0.1 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0.16 0.16 0.15 0.06 0 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Mitochondria 0.27 0.2 0.21 0.52 0.36 0.68 – 0.64 0.81 0.78 0.83 0.8 0.1 0 0 0.12 0.08 0.13 0.47 0.59 0.41 0.45 0.45 0.35
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 – 0.09 0 0.06 0 0.05 0 0 0 0.15 0.18 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.17 0.1 0.15 0.05 0.08 0 0 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 0 0 0 0 0 – 0 0 0 0 0 2 0 0 4 3 3 9 3 3 4 9 16
Bud 2 0 1 7 10 15 – 22 11 17 11 23 9 3 2 7 9 4 1 1 0 2 8 13
Bud Neck 3 0 3 0 3 0 – 8 0 3 0 1 0 1 0 0 1 2 2 1 0 3 7 10
Bud Site 0 0 1 0 4 1 – 12 2 5 2 14 0 0 0 0 2 1 – – – – – –
Cell Periphery 2 1 2 1 1 1 – 0 1 0 1 2 0 1 2 4 3 5 1 1 3 1 1 2
Cytoplasm 233 75 115 147 194 144 – 188 83 80 92 147 313 583 659 19 35 25 126 20 50 59 117 160
Endoplasmic Reticulum 9 1 0 0 2 0 – 1 1 1 2 0 3 2 0 7 9 8 2 1 2 2 3 1
Endosome 0 2 0 0 5 0 – 1 0 0 0 0 19 20 8 13 15 15 24 6 2 4 22 25
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 6 0 6 1 1 0 2 2 4
Mitochondria 88 20 28 119 113 212 – 302 217 193 277 422 36 25 13 10 7 13 200 82 56 80 189 163
Nucleus 1 1 1 0 0 2 – 6 2 3 2 1 0 3 1 2 5 3 0 0 1 0 1 1
Nuclear Periphery 1 0 0 0 1 0 – 2 4 0 2 2 0 0 0 0 3 0 0 0 0 0 2 0
Nucleolus 0 0 0 1 0 2 – 2 0 2 3 1 0 1 0 1 2 0 1 0 0 0 1 0
Peroxisomes 0 0 0 0 8 2 – 2 0 3 0 3 10 2 7 3 0 6 1 1 0 2 2 1
SpindlePole 2 3 1 4 17 8 – 43 5 15 14 28 8 4 1 12 16 9 14 3 4 3 16 13
Vac/Vac Membrane 8 1 0 1 7 6 – 9 6 3 1 5 7 9 10 14 9 15 23 10 5 6 30 40
Unique Cell Count 321 98 131 231 316 310 469 268 249 332 526 373 638 684 82 91 99 426 141 139 179 424 470
Labelled Cell Count 352 104 152 280 365 393 598 332 325 407 649 407 654 703 102 119 115 426 141 139 179 424 470


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.1 2.8 3.0 3.7 2.9 – 2.8 2.6 2.8 2.6 2.5 4.1 4.3 4.2 6.3 5.4 6.2 4.1 4.6 4.4
Std Deviation (1e-4) 0.5 0.7 0.8 1.4 1.7 1.1 – 1.0 1.4 1.3 1.2 0.9 1.1 1.3 1.3 1.5 1.2 1.6 1.5 1.9 1.5
Intensity Change (Log2) – – – 0.09 0.41 0.02 – 0.02 -0.09 -0.02 -0.11 -0.16 0.56 0.63 0.59 1.16 0.95 1.15 0.53 0.7 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 4.9 – 4.6