Standard name
Human Ortholog
Description Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0.05 0 0.05 0 0 0 0.09 0.09 0 0 0 0 0 0 0
Cytoplasm 0.9 0.94 0.94 0.92 0.86 0.82 0.74 – 0.69 0.65 0.54 0.61 0.98 0.99 0.98 0.88 0.9 0.96 0.85 0.92 0.92 0.87 0.89 0.87
Endoplasmic Reticulum 0 0.05 0.06 0.07 0 0 0.08 – 0.07 0.1 0 0.09 0 0 0 0 0 0 0 0 0.06 0.06 0.09 0.09
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0 – 0.25 0.12 0.37 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.14 0 0.16 – 0.12 0.16 0.14 0.1 0 0 0 0.1 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 3 4 – 0 1 1 2 0 0 0 0 0 0 0 2 0 1 0 1
Bud 0 0 0 0 0 2 0 – 1 1 0 1 0 1 0 0 0 0 0 1 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 2
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 7 2 5 2 6 9 – 3 9 2 7 4 15 13 14 11 4 0 0 0 0 0 1
Cytoplasm 112 428 119 195 155 202 198 – 125 126 51 86 391 744 723 140 105 107 111 433 131 256 386 504
Endoplasmic Reticulum 3 21 8 14 2 8 21 – 13 19 3 13 11 15 8 3 2 4 3 16 7 17 37 54
Endosome 0 0 0 0 1 0 1 – 0 0 0 0 1 0 0 0 0 0 3 3 0 0 0 1
Golgi 2 0 0 0 0 0 3 – 0 1 0 0 0 0 0 1 0 0 2 0 0 3 1 1
Mitochondria 5 1 0 0 1 32 11 – 45 24 35 42 0 0 0 4 3 0 1 1 0 2 1 1
Nucleus 2 7 0 2 3 2 1 – 0 2 1 3 0 0 1 2 2 0 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 – 1 1 0 2 0 0 2 0 0 0 1 1 0 0 0 3
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 0 2 25 9 42 – 22 30 13 14 2 1 4 16 4 0 2 4 0 5 1 4
Unique Cell Count 125 453 127 211 180 246 266 181 193 95 140 401 754 740 160 117 111 131 472 144 294 435 580
Labelled Cell Count 128 464 129 218 189 265 290 210 214 106 170 409 776 752 180 127 116 131 472 144 294 435 580


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 10.0 10.7 10.6 11.7 7.7 10.3 – 9.0 9.4 8.5 8.7 11.3 11.0 11.1 11.7 12.2 11.3 10.3 11.1 11.9
Std Deviation (1e-4) 2.1 1.4 1.4 1.6 1.8 1.9 1.6 – 1.8 1.8 1.6 1.8 1.6 1.5 1.4 2.5 2.8 2.4 1.9 1.5 2.1
Intensity Change (Log2) – – – -0.01 0.13 -0.47 -0.05 – -0.25 -0.19 -0.33 -0.3 0.08 0.05 0.06 0.13 0.2 0.09 -0.06 0.05 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 2.6 2.7 0
Cytoplasm -0.4 -2.1 -3.1 -4.5 – -5.2 -5.9 -7.0 -6.2 2.1 3.7 2.5 -1.8 -1.1 0.9
Endoplasmic Reticulum 0.1 0 -1.4 0.6 – 0.3 1.1 0 0.9 0 0 0 0 0 -0.9
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.3 0 – 6.1 4.1 7.5 6.7 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.4 0 4.7 – 4.1 4.7 4.3 3.7 0 0 0 3.7 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7101 3.829 3.8488 3.8384 3.4833 3.9664 6.515 6.7961 6.479 5.8367 5.8156 6.2844 8.2229 8.3018 8.3816 8.4158 8.0795 8.3326
Actin 0.008 0.0054 0.0023 0.0022 0.0006 0.0004 0.0004 0.0006 0.0008 0.0006 0.0005 0.0009 0.0052 0.0017 0.0014 0.0043 0.0007 0.0009
Bud 0.0003 0.0017 0.0012 0.0002 0.0001 0.0001 0.0001 0.0003 0.0004 0.0003 0.0001 0.0001 0.0003 0.0009 0.0002 0.0008 0.0001 0.0001
Bud Neck 0.0007 0.0012 0.0017 0.0015 0.001 0.001 0.0003 0.0012 0.0007 0.001 0.0008 0.001 0.0016 0.0004 0.0003 0.0075 0.0003 0.0009
Bud Periphery 0.0004 0.0011 0.0007 0.0001 0 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0004 0.0001 0.0005 0 0.0001
Bud Site 0.0017 0.0174 0.0138 0.0018 0.0001 0.0001 0.0004 0.0053 0.0011 0.0005 0.0001 0.0001 0.0048 0.0097 0.0006 0.0034 0.0001 0.0001
Cell Periphery 0.0003 0.0004 0.0004 0.0001 0.0001 0 0.0002 0.0003 0.0001 0.0001 0.0001 0 0.0004 0.0004 0.0002 0.0004 0 0.0001
Cytoplasm 0.8363 0.8727 0.8518 0.8465 0.8543 0.8721 0.8549 0.9133 0.8821 0.8491 0.8525 0.8708 0.7701 0.9104 0.8832 0.8283 0.9075 0.8867
Cytoplasmic Foci 0.0149 0.0134 0.013 0.0139 0.0166 0.0191 0.0105 0.0111 0.0098 0.0188 0.0236 0.0105 0.0104 0.0076 0.0085 0.0171 0.0168 0.0104
Eisosomes 0.0004 0.0001 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0529 0.0199 0.053 0.0252 0.0336 0.0445 0.0684 0.0218 0.049 0.0651 0.0371 0.0624 0.1246 0.0316 0.059 0.0887 0.0229 0.0544
Endosome 0.0221 0.0129 0.0148 0.0305 0.0284 0.0257 0.0143 0.0127 0.0151 0.018 0.0419 0.0196 0.0175 0.0086 0.0119 0.0245 0.0293 0.0156
Golgi 0.0028 0.0012 0.001 0.0018 0.0005 0.0011 0.0009 0.0008 0.0008 0.0011 0.0009 0.0008 0.0021 0.001 0.0012 0.0019 0.0029 0.0008
Lipid Particles 0.0031 0.0009 0.0003 0.0013 0.0003 0.0022 0.0007 0.0003 0.0005 0.0007 0.0007 0.0002 0.0019 0.0004 0.0003 0.0005 0.0004 0.0002
Mitochondria 0.0019 0.0019 0.001 0.0006 0.0003 0.0032 0.0007 0.0004 0.0009 0.0007 0.0008 0.0011 0.001 0.0012 0.0007 0.0008 0.0006 0.0008
None 0.036 0.0363 0.028 0.0438 0.0526 0.0183 0.0363 0.016 0.0182 0.0249 0.0273 0.0182 0.0356 0.0139 0.0229 0.0054 0.0118 0.0208
Nuclear Periphery 0.0055 0.0026 0.0051 0.0051 0.0029 0.0032 0.0041 0.0024 0.008 0.0046 0.0032 0.0044 0.0095 0.004 0.0045 0.0062 0.0024 0.0026
Nucleolus 0.0003 0.0003 0.0002 0.001 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0.0004 0.0001 0 0 0 0
Nucleus 0.0031 0.0043 0.0054 0.0058 0.0027 0.0037 0.0035 0.0074 0.0065 0.0064 0.0038 0.005 0.0055 0.0039 0.0024 0.0043 0.0018 0.0027
Peroxisomes 0.001 0.0005 0.0002 0.0002 0.0001 0.0004 0.0002 0.0001 0.0001 0.001 0.0002 0.0001 0.0014 0.0001 0.0001 0.0002 0.0001 0.0001
Punctate Nuclear 0.0024 0.0021 0.0021 0.0125 0.0009 0.0008 0.0004 0.0009 0.0019 0.0012 0.0007 0.0016 0.003 0.0012 0.0004 0.0009 0.0005 0.0003
Vacuole 0.0051 0.0034 0.0035 0.0048 0.0042 0.003 0.0031 0.0047 0.0031 0.005 0.0046 0.0024 0.0036 0.0021 0.0018 0.0036 0.0014 0.0018
Vacuole Periphery 0.0008 0.0004 0.0006 0.0009 0.0007 0.0008 0.0004 0.0004 0.0006 0.0006 0.0009 0.0006 0.0007 0.0004 0.0004 0.0007 0.0004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.6493 13.9171 20.6542 24.9139 25.2313 13.6147 21.9869 11.8717 25.0976 22.4511
Translational Efficiency 3.0266 2.5011 1.2569 1.1562 1.4236 2.5215 1.3492 2.2934 1.3624 1.8132

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1885 993 297 886 1701 2417 937 630 3586 3410 1234 1516

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 971.63 1052.11 1119.55 1052.18 904.67 1085.38 1125.41 1211.43 939.87 1075.69 1124.00 1118.36
Standard Deviation 132.54 185.13 134.72 150.60 132.32 150.26 152.44 161.28 136.59 161.90 148.39 173.85
Intensity Change Log 2 — 0.114807 0.204440 0.114903 — 0.262737 0.314987 0.421248 — 0.188027 0.258799 0.270735

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000247 0.000359 0.000561 0.000261 0.000300 0.000574 0.000322 0.000746 0.000272 0.000512 0.000379 0.000463
Bud Neck 0.002110 0.001455 0.002757 0.002728 0.001614 0.002700 0.001954 0.002125 0.001875 0.002337 0.002147 0.002478
Bud Site 0.011867 0.016879 0.016707 0.012299 0.011107 0.016813 0.011147 0.022395 0.011507 0.016832 0.012485 0.016495
Cell Periphery 0.000249 0.000190 0.000194 0.000066 0.000213 0.000141 0.000119 0.000136 0.000232 0.000155 0.000137 0.000095
Cytoplasm 0.569394* 0.663345* 0.597292* 0.551513* 0.617014* 0.666397* 0.708921* 0.739649* 0.591982* 0.665508* 0.682054* 0.629696*
Cytoplasmic Foci 0.240459* 0.163958 0.111714 0.071251 0.213967* 0.170890 0.090305 0.086362 0.227892* 0.168871 0.095458 0.077531
Eisosomes 0.000045 0.000011 0.000027 0.000015 0.000024 0.000024 0.000015 0.000012 0.000035 0.000020 0.000018 0.000014
Endoplasmic Reticulum 0.054513 0.024790 0.005902 0.002045 0.038112 0.021202 0.002530 0.001084 0.046733 0.022247 0.003341 0.001646
Endosome 0.017088 0.018555 0.024310 0.011681 0.016584 0.014346 0.010077 0.007183 0.016849 0.015572 0.013503 0.009812
Golgi 0.018284 0.008767 0.001106 0.001691 0.012221 0.011188 0.000790 0.000708 0.015408 0.010483 0.000866 0.001282
Lipid Particles 0.007948 0.004339 0.007983 0.002680 0.005987 0.004813 0.003999 0.004628 0.007018 0.004675 0.004957 0.003490
Mitochondria 0.002040 0.000389 0.000066 0.000160 0.001888 0.001881 0.000040 0.000041 0.001968 0.001447 0.000046 0.000110
Mitotic Spindle 0.000715 0.002492 0.009122 0.011667 0.000821 0.003774 0.004519 0.006912 0.000765 0.003400 0.005627 0.009691
None 0.001037 0.001156 0.000380 0.001178 0.002455 0.001297 0.001098 0.000985 0.001710 0.001256 0.000925 0.001098
Nuclear Periphery 0.000197 0.000228 0.001568 0.000711 0.000261 0.000167 0.000311 0.000154 0.000227 0.000185 0.000613 0.000479
Nuclear Periphery Foci 0.001117 0.006130 0.023185 0.005825 0.002160 0.001115 0.015681 0.010158 0.001612 0.002575 0.017487 0.007626
Nucleolus 0.000236 0.000291 0.000124 0.000386 0.000222 0.000247 0.000132 0.000127 0.000230 0.000260 0.000130 0.000278
Nucleus 0.011063 0.009447 0.017489 0.022363 0.011635 0.012413 0.023170 0.014770 0.011335 0.011549 0.021803 0.019208
Peroxisomes 0.000773 0.000841 0.000443 0.001430 0.000583 0.000976 0.000436 0.002011 0.000683 0.000937 0.000437 0.001671
Vacuole 0.050372 0.069550 0.177863 0.299135* 0.052079 0.061356 0.123981 0.099512 0.051181 0.063742 0.136950 0.216178*
Vacuole Periphery 0.010244 0.006830 0.001206 0.000916 0.010753 0.007686 0.000454 0.000301 0.010485 0.007437 0.000635 0.000661

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.38 -4.35 0.40 3.02 4.27 -1.56 -0.45 -1.52 0.15 -1.43 -1.99 -2.71 -1.27 1.13 0.29
Bud Neck 3.13 -2.41 -0.95 -2.78 1.10 -3.85 -2.59 -2.29 2.14 0.12 -2.33 -2.77 -1.94 0.24 0.08
Bud Site -3.48 -2.14 1.54 4.06 2.82 -4.69 0.08 -3.75 -0.70 -3.64 -5.71 -0.79 -1.79 2.66 -0.95
Cell Periphery 4.17 3.59 16.66 13.22 9.45 5.42 7.32 6.20 2.10 -0.29 8.21 10.28 16.04 11.32 7.49
Cytoplasm -8.59 -0.67 4.20 10.66 3.21 -5.62 -5.49 -6.46 -2.54 -1.45 -11.11 -6.89 -0.04 8.17 5.68
Cytoplasmic Foci 10.87 17.63 32.27 15.66 5.98 7.26 22.19 20.79 15.72 0.63 13.31 31.14 37.81 24.30 4.67
Eisosomes 16.14 6.21 13.78 -4.72 5.83 0.12 5.16 7.17 6.27 3.71 8.03 11.25 15.06 4.60 5.19
Endoplasmic Reticulum 7.10 14.83 17.01 8.31 3.00 5.72 14.72 15.27 11.75 2.74 9.88 21.49 22.64 14.35 3.90
Endosome -1.02 -1.86 8.88 6.65 5.33 2.10 7.38 10.46 9.16 3.44 1.57 5.31 13.26 10.27 5.01
Golgi 8.89 24.46 19.42 7.79 0.03 1.05 19.99 20.46 12.72 1.11 6.33 31.73 25.99 13.45 -0.10
Lipid Particles 7.05 0.65 11.95 4.31 5.54 2.69 4.04 2.59 0.78 -0.40 7.03 5.28 10.12 4.18 4.11
Mitochondria 7.54 9.62 9.38 3.48 -1.61 0.01 4.30 4.30 8.57 0.35 1.87 8.34 8.18 8.82 -2.32
Mitotic Spindle -2.53 -2.73 -4.35 -3.25 0.14 -3.83 -4.35 -4.05 -2.39 -1.50 -4.47 -5.04 -5.91 -3.71 -1.41
None -0.29 4.12 0.62 0.62 -2.65 2.72 3.52 3.56 1.68 0.42 1.79 3.87 3.38 1.78 -0.31
Nuclear Periphery -0.54 -1.53 -5.31 -5.65 1.05 2.34 -3.31 0.84 -2.08 5.47 1.12 -2.05 -4.78 -6.56 0.96
Nuclear Periphery Foci -6.91 -10.71 -10.39 0.69 8.42 3.38 -12.45 -7.71 -8.86 4.28 -3.50 -16.52 -11.60 -8.98 9.72
Nucleolus -0.88 4.92 -1.39 -0.51 -2.69 -0.99 5.25 5.37 4.36 0.81 -1.20 7.13 -0.62 0.09 -2.55
Nucleus 1.57 -5.63 -10.68 -10.00 -1.87 -1.12 -11.83 -4.55 -4.17 6.35 -0.59 -13.90 -11.11 -10.60 3.60
Peroxisomes -0.42 7.02 -0.53 -0.38 -1.15 -6.17 3.24 -1.37 -0.62 -1.70 -3.90 6.49 -1.24 -0.58 -1.93
Vacuole -4.61 -12.85 -30.33 -26.69 -9.67 -3.35 -16.79 -13.12 -11.95 0.72 -5.80 -21.36 -30.72 -28.50 -11.28
Vacuole Periphery 3.73 13.55 15.54 9.03 1.44 3.60 15.57 15.80 13.81 3.39 4.91 21.71 22.08 16.12 1.11
Standard name
Human Ortholog
Description Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Gfd1

Gfd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gfd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available