Standard name
Human Ortholog
Description Phosphoribosylpyrophosphate amidotransferase (PRPPAT); catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; also known as amidophosphoribosyltransferase
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09 0.05 0.11 0.12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.18 0.14 0.08 0.09 0.05 0.06
Bud Site 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.06 0.05 0.08 0.13 0.08 0.12 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.18 0.17 0.58 0.9 0.91 0.81 0.77 0.79 0.73 0.81 0.73 0.51 0.8 0.89 0.37 0.29 0.35 0.05 0.07 0.05 0.06 0.08 0.05
Endoplasmic Reticulum 0 0 0 0 0.06 0 0 0.08 0.08 0.05 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.07 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0.05 0
Golgi 0 0.06 0 0.09 0 0 0 0 0 0 0 0 0.15 0.11 0.07 0.23 0.27 0.21 0 0 0 0 0 0
Mitochondria 0.11 0.09 0.07 0 0 0 0.06 0.07 0.09 0.12 0.08 0.12 0.06 0 0 0.09 0 0.09 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.78 0.68 0.54 0.13 0 0 0 0 0 0 0 0 0.23 0 0 0.24 0.3 0.37 0.11 0.22 0.05 0.13 0.05 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
SpindlePole 0 0 0.27 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.09 0 0.46 0.27 0.53 0.49 0.52 0.55
Vac/Vac Membrane 0 0 0 0.09 0 0 0 0.05 0 0.05 0 0 0 0 0 0.07 0.13 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 5 5 2 3 5 4 11 6 6 5 10 12 4 3 5 2 1 2 8 3 2 7 9
Bud 5 2 4 1 2 2 2 5 2 7 0 6 8 5 6 3 0 1 6 24 13 9 41 34
Bud Neck 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 31 41 12 17 17 18
Bud Site 0 1 7 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 2 9 10 19 20 16 20 27 3 8 11 6 1 1 0 0 0 0 0 0
Cytoplasm 18 35 18 26 86 141 166 195 124 143 135 147 109 244 391 123 50 63 7 20 8 11 30 15
Endoplasmic Reticulum 0 3 1 0 6 5 9 20 12 10 13 12 7 8 7 10 3 5 2 5 1 0 3 0
Endosome 0 2 8 5 0 0 1 1 0 0 0 0 1 1 0 6 6 0 5 18 8 3 19 10
Golgi 0 12 1 4 2 1 2 5 0 0 0 0 32 33 29 75 46 38 1 1 2 4 3 2
Mitochondria 15 17 8 2 0 6 12 17 14 23 13 24 13 0 0 29 6 17 1 2 1 2 2 1
Nucleus 0 1 0 0 0 1 1 0 1 1 1 0 1 0 0 0 0 1 0 2 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 105 131 58 6 0 0 2 1 0 2 0 0 48 11 5 81 51 67 19 66 8 24 19 9
Peroxisomes 1 1 1 1 1 0 0 0 0 0 0 0 4 3 2 2 0 2 5 7 5 6 18 11
SpindlePole 4 2 29 1 0 0 1 1 0 0 0 0 5 1 2 41 15 8 79 82 80 89 202 160
Vac/Vac Membrane 0 8 2 4 0 2 9 13 3 10 1 5 2 3 1 22 22 5 2 9 0 6 8 3
Unique Cell Count 134 192 107 45 96 155 205 253 157 197 166 200 213 305 438 332 171 179 173 302 151 183 385 290
Labelled Cell Count 151 222 145 52 102 173 219 288 182 219 188 231 245 321 457 403 204 210 173 302 151 183 385 290


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 38.5 43.8 23.9 23.3 21.9 18.8 15.6 16.2 16.1 14.2 15.1 14.8 55.6 57.3 59.4 40.3 46.2 51.4 40.6 39.3 38.4
Std Deviation (1e-4) 11.6 15.8 9.1 8.8 6.3 5.1 4.4 4.6 3.8 3.8 3.5 3.4 16.7 16.0 16.1 12.9 16.6 20.6 14.9 14.5 14.0
Intensity Change (Log2) – – – -0.04 -0.13 -0.35 -0.61 -0.56 -0.57 -0.75 -0.67 -0.69 1.22 1.26 1.31 0.75 0.95 1.1 0.76 0.72 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0.1 0.4 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2.9 3.8 3.0 3.7 4.0 0 0 0 0 0 0
Cytoplasm 5.1 10.4 12.1 10.9 10.6 9.9 9.3 10.5 9.5 5.9 11.7 15.5 3.9 2.3 3.3
Endoplasmic Reticulum 0 0 0 0 2.6 2.5 0 2.5 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 -1.5 0
Golgi 0 0 0 0 0 0 0 0 0 3.9 3.2 2.3 5.1 5.6 4.8
Mitochondria 0 0 -1.3 -0.6 -0.3 0.4 1.2 0.1 1.2 -0.5 0 0 0.4 -1.5 0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -4.7 -8.5 -10.4 -11.3 -12.6 -10.4 -11.1 -10.7 -11.6 -5.7 -12.1 -15.4 -5.8 -4.1 -2.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -3.5 -5.5 -6.9 -7.6 -8.4 -6.9 -7.7 -7.1 -7.7 -6.8 -9.2 -10.7 -3.6 -4.1 -5.5
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.9 3.2 0