ORF
Human Ortholog
Description Putative methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; not an essential gene; YMR310C has a paralog, YGR283C, that arose from the whole genome duplication
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ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.06 0.09 0.06 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0.11 0.17 0.08 0.05 0.07 0 0.08 0 0.06 0.15 0.08 0.07 0 0 0 0 0 0 0.05 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.22 0.25 0.5 0.31 0.5 0.5 0.46 0.6 0.46 0.43 0.38 0.4 0.4 0.34 0.36 0.24 0.16 0.21 0.14 0.1 0.14 0.11 0.17 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0.2 0.06 0 0.07 0.09 0 0 0 0 0 0 0 0.2 0.26 0.22 0.27 0.23 0.12 0.12 0.1 0.14 0.12 0.15
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.76 0.14 0.06 0.11 0.05 0.12 0.42 0.27 0.52 0.51 0.68 0.53 0 0 0 0.1 0.08 0.12 0.05 0.06 0.14 0.07 0.05 0
Nucleus 0.05 0.15 0.14 0 0 0 0 0 0 0 0 0 0.17 0 0 0.14 0.2 0.18 0.09 0.15 0.05 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.16 0.07 0.07 0.1 0.08 0.07 0.09 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.06 0.19 0.5 0.34 0.34 0.19 0.19 0.16 0.15 0.09 0.22 0.32 0.42 0.38 0.42 0.42 0.29 0.19 0.19 0.2 0.39 0.35 0.35
Vac/Vac Membrane 0.07 0.39 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.11 0.22 0.21 0.17 0.08 0.08 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 2 0 0 2 0 1 0 0 0 0 2 0 0 1 0
Bud 1 0 1 1 3 5 18 15 24 16 20 13 1 0 0 3 1 2 1 2 1 0 4 5
Bud Neck 1 0 6 12 26 15 18 16 11 22 7 14 26 14 15 1 2 0 2 0 1 6 7 10
Bud Site 0 0 0 3 0 4 11 5 8 11 5 5 0 0 0 0 5 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 28 27 48 33 76 98 152 145 122 114 87 92 69 62 73 32 26 21 25 16 23 15 38 26
Endoplasmic Reticulum 0 2 0 0 0 0 0 2 0 0 0 0 2 1 2 5 6 7 0 0 2 0 1 1
Endosome 1 21 6 2 10 17 4 5 1 3 0 3 5 37 51 29 42 23 21 19 15 20 27 34
Golgi 1 1 1 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 0 2
Mitochondria 99 15 6 12 8 24 137 64 139 133 155 123 2 1 2 13 13 12 9 9 23 10 12 10
Nucleus 7 16 13 4 1 1 6 7 3 7 5 4 29 8 5 18 31 18 16 24 8 2 3 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 2 2
Nucleolus 2 1 0 1 0 0 0 0 1 1 0 1 0 1 0 10 25 7 11 15 13 10 20 13
Peroxisomes 0 0 0 0 0 6 4 2 1 0 0 2 0 1 6 0 2 0 0 0 0 0 0 1
SpindlePole 5 6 18 54 51 66 62 46 43 39 21 50 55 76 75 55 66 28 33 30 32 56 78 80
Vac/Vac Membrane 9 42 4 2 1 1 3 5 1 4 2 2 2 3 3 9 10 11 38 33 27 12 17 23
Unique Cell Count 130 107 96 107 151 196 330 240 265 263 228 231 174 182 200 131 158 98 177 162 166 145 227 228
Labelled Cell Count 154 131 103 124 177 237 415 313 354 354 303 309 193 204 233 177 230 131 177 162 166 145 227 228


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.5 4.9 4.7 4.8 4.1 3.5 4.0 3.2 3.3 3.0 3.4 4.9 5.2 5.0 6.8 6.9 6.2 4.7 5.2 5.6
Std Deviation (1e-4) 0.4 0.8 1.5 1.8 1.9 2.1 1.8 1.7 1.6 1.3 1.4 1.2 1.0 1.2 1.3 1.7 2.1 1.8 1.3 1.8 2.1
Intensity Change (Log2) – – – -0.04 -0.03 -0.26 -0.48 -0.27 -0.59 -0.55 -0.68 -0.52 -0.0 0.11 0.04 0.47 0.51 0.35 -0.06 0.1 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 0.9 0.7 1.8