Standard name
Human Ortholog
Description Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0.1 0.07 0.1 0.07 0.08 0.13 0.08 0.09 0.13 0.07 0.13 0.17 0.21 0.1 0.12 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0.05 0 0.14 0 0.1 0.2 0.13 0.37 0.24 0.49 0.38 0 0 0 0.1 0 0.08 0 0 0.05 0 0.06 0.09
Nucleus 0.09 0.09 0.29 0.33 0.3 0.25 0.35 0.39 0.3 0.36 0.26 0.33 0.2 0.23 0.28 0.14 0.08 0.24 0 0 0 0.11 0.13 0.13
Nuclear Periphery 0.53 0.59 0.24 0.35 0.25 0.28 0.3 0.19 0.19 0.16 0.16 0.15 0.36 0.38 0.29 0.4 0.54 0.24 0.82 0.84 0.71 0.6 0.47 0.35
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.46 0.59 0.62 0.31 0.44 0.5 0.38 0.33 0.34 0.37 0.24 0.31 0.57 0.38 0.4 0.4 0.58 0.73 0.05 0 0.08 0.11 0.19 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0
Bud 0 0 0 0 0 0 2 4 6 3 1 5 0 0 0 0 0 0 0 1 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0
Cytoplasm 16 5 4 7 19 13 19 39 19 23 26 16 19 34 47 6 3 2 2 0 0 4 3 5
Endoplasmic Reticulum 5 4 0 0 1 2 0 0 0 0 4 0 1 4 1 4 1 1 2 0 2 1 4 2
Endosome 1 0 0 2 3 1 4 4 2 6 3 4 2 2 2 1 1 0 3 3 0 1 5 4
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 2 0 1 0 0 0
Mitochondria 69 7 0 15 5 19 45 38 93 58 95 83 1 5 2 6 1 3 11 3 4 4 12 19
Nucleus 24 12 12 34 57 47 81 116 75 89 50 72 30 46 64 9 2 9 3 3 3 12 27 28
Nuclear Periphery 137 79 10 36 48 53 69 56 47 39 31 32 54 78 65 25 13 9 235 127 61 68 95 75
Nucleolus 2 0 0 0 0 1 0 1 1 1 1 2 0 1 0 5 1 2 1 1 0 1 7 4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 0 2 1 2 1 4 1 2 3 0 0 0 3 1 1 2 3 5
Vac/Vac Membrane 119 79 26 32 84 93 88 99 86 91 46 69 84 78 91 25 14 27 12 6 6 12 39 58
Unique Cell Count 257 133 42 104 192 186 230 299 251 244 194 220 148 203 227 63 24 37 287 152 87 114 204 213
Labelled Cell Count 373 186 52 126 219 230 309 359 330 312 258 288 192 254 276 81 36 55 287 152 87 114 204 213


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.6 6.2 6.0 7.2 5.8 5.6 6.1 5.0 5.1 4.8 4.7 6.5 6.2 6.3 7.6 8.9 7.7 5.9 6.4 6.7
Std Deviation (1e-4) 0.7 0.9 0.9 1.9 2.3 2.0 1.8 2.2 1.6 1.4 2.0 1.5 1.2 1.4 1.6 2.0 1.6 1.6 1.2 1.6 1.2
Intensity Change (Log2) – – – -0.04 0.21 -0.09 -0.15 -0.02 -0.33 -0.27 -0.38 -0.39 0.06 -0.01 0.03 0.29 0.52 0.3 -0.07 0.05 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.6 0 0 0.7 0 0.6 1.2 1.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 0 4.8 3.5 0 0 0 0 0 0 0 0
Nucleus 0.5 0.1 -0.4 0.8 1.3 0.2 1.0 -0.4 0.5 -1.1 -0.8 0 -1.8 -1.9 -0.4
Nuclear Periphery 1.3 0.2 0.6 0.8 -0.8 -0.8 -1.2 0 0 1.5 1.8 0.6 1.7 2.5 0.1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.5 -2.1 -1.4 -2.9 -3.6 -3.4 -3.0 -4.9 -3.8 -0.6 -2.8 -2.6 -2.2 -0.3 1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0165 0.9696 0.7181 0.3622 0.4742 0.5814 1.3891 1.5267 1.3889 1.1586 0.8981 1.342 1.2065 0.942 0.9717 0.8487 1.0206 0.7545
Actin 0.0013 0.0008 0.0084 0.0005 0.005 0.0001 0.0123 0.0002 0.0002 0.0017 0.0005 0.0004 0.0086 0.0001 0.0056 0.0001 0.02 0.0012
Bud 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0004 0 0 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0015 0.0001
Bud Neck 0.0004 0.0032 0.0002 0.0001 0.0003 0 0.0012 0 0 0.0001 0.0001 0.0001 0.0009 0 0.0004 0 0.0003 0.0001
Bud Periphery 0.0001 0.0001 0.0005 0.0005 0.0002 0.0001 0.0007 0 0.0001 0.0002 0.0001 0.0002 0.0002 0 0.0002 0 0.005 0.0003
Bud Site 0.0004 0.0027 0.0017 0.0002 0.0002 0 0.0046 0 0 0.0002 0.0002 0 0.0008 0 0.0057 0 0.0013 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0.0002 0 0 0.0003 0 0 0.0001 0 0 0.0001 0 0.0001 0 0.0002 0
Cytoplasm 0.0032 0.0031 0.0027 0.0021 0.0021 0.0007 0.008 0.0031 0.0007 0.0008 0.0007 0.001 0.0022 0.0107 0.0043 0.0001 0.0018 0.0035
Cytoplasmic Foci 0.0054 0.0005 0.0029 0.0013 0.0019 0.0002 0.0138 0.0001 0.005 0.0058 0.0058 0.0006 0.0162 0.0001 0.0016 0 0.0048 0.0028
Eisosomes 0.0001 0 0.0001 0 0.0004 0 0.0002 0 0 0 0 0 0.0001 0 0.0001 0 0.0003 0.0001
Endoplasmic Reticulum 0.0102 0.0116 0.0114 0.0111 0.0037 0.003 0.0105 0.0167 0.018 0.035 0.004 0.0048 0.0092 0.0179 0.0095 0.0084 0.005 0.0061
Endosome 0.0255 0.0017 0.0074 0.0198 0.0144 0.002 0.0179 0.0011 0.0079 0.0044 0.0229 0.0037 0.0174 0.0012 0.0059 0.0018 0.0346 0.0061
Golgi 0.0041 0.0002 0.0025 0.0025 0.0052 0.0004 0.0051 0 0.001 0.0024 0.0193 0.003 0.0056 0 0.0022 0.0001 0.0202 0.0011
Lipid Particles 0.0326 0.0006 0.0056 0.0007 0.0052 0.0034 0.0169 0.0002 0.0096 0.0116 0.0028 0.0076 0.012 0.0002 0.0058 0.0003 0.0311 0.0032
Mitochondria 0.0023 0.0009 0.0093 0.0149 0.0022 0.0009 0.0112 0.0006 0.0046 0.0615 0.0064 0.0131 0.0031 0.0006 0.006 0.002 0.0204 0.004
None 0.0086 0.0011 0.0082 0.0003 0.027 0.0007 0.0172 0.0135 0.0038 0.0007 0.0005 0.0053 0.0016 0.0009 0.0013 0.0002 0.0089 0.0026
Nuclear Periphery 0.8523 0.8766 0.8768 0.8944 0.7882 0.9399 0.8194 0.8857 0.8888 0.779 0.8595 0.9043 0.854 0.8644 0.8905 0.9382 0.7532 0.9135
Nucleolus 0.0009 0.001 0.0002 0.0001 0.0014 0.0003 0.0008 0.0004 0.0002 0.0001 0.0004 0.0006 0.0014 0.0016 0.0003 0.0001 0.0008 0.0004
Nucleus 0.0157 0.0528 0.0131 0.0114 0.1198 0.0297 0.0134 0.0312 0.0089 0.0153 0.0379 0.0318 0.0178 0.0675 0.0171 0.0082 0.0614 0.0327
Peroxisomes 0.0025 0.0001 0.0035 0.0001 0.0008 0.0001 0.0101 0 0.0037 0.0445 0.0011 0.0008 0.011 0 0.0013 0 0.0083 0.001
Punctate Nuclear 0.0043 0.0042 0.0015 0.0003 0.0042 0.0011 0.0026 0.0136 0.0005 0.0012 0.0209 0.0031 0.0065 0.0034 0.0009 0.0002 0.0029 0.0056
Vacuole 0.0032 0.0013 0.0021 0.0041 0.001 0.0019 0.0045 0.0011 0.0019 0.002 0.001 0.002 0.0018 0.0015 0.0017 0.0008 0.0024 0.0012
Vacuole Periphery 0.0268 0.0375 0.0418 0.0354 0.0168 0.0155 0.0291 0.0324 0.0449 0.0334 0.0157 0.0175 0.0295 0.0297 0.0394 0.0393 0.0157 0.0144

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.1253 9.7132 9.0095 14.9844 11.2702 7.0587 16.9412 14.9142 15.5702 19.4964
Translational Efficiency 0.6558 0.8066 0.8895 0.5556 0.6109 1.2652 0.5009 0.4988 0.6522 0.5515

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids
Localization
Cell Percentages nuclear periphery (66%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Asi3

Asi3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Asi3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available