Standard name | |
---|---|
Human Ortholog | |
Description | S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 2 | 1 | – | 0 | 0 | 6 | 4 | 3 | 4 | 11 | 14 |
Bud | 7 | 1 | 3 | 2 | 2 | 2 | 16 | 16 | 15 | 10 | 14 | 13 | 1 | 0 | 0 | – | 0 | 3 | 2 | 0 | 0 | 1 | 3 | 10 |
Bud Neck | 4 | 0 | 4 | 5 | 15 | 4 | 10 | 10 | 5 | 9 | 2 | 5 | 12 | 7 | 6 | – | 4 | 1 | 1 | 2 | 0 | 1 | 5 | 11 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 5 | 2 | 4 | 3 | 0 | 0 | 0 | 0 | – | 1 | 2 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 4 | 1 | 1 | 0 | 4 | 1 | 1 | 1 | 0 | 0 | 2 | 2 | 10 | – | 6 | 0 | 1 | 0 | 0 | 0 | 2 | 6 |
Cytoplasm | 117 | 90 | 99 | 154 | 194 | 132 | 132 | 124 | 115 | 82 | 83 | 122 | 412 | 474 | 587 | – | 45 | 33 | 38 | 22 | 21 | 30 | 78 | 91 |
Endoplasmic Reticulum | 4 | 5 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 2 | – | 8 | 5 | 7 | 1 | 4 | 2 | 4 | 5 |
Endosome | 0 | 6 | 2 | 0 | 6 | 1 | 2 | 4 | 0 | 1 | 0 | 0 | 3 | 1 | 5 | – | 5 | 7 | 14 | 13 | 7 | 5 | 18 | 12 |
Golgi | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 1 | 5 | 1 | 3 | 0 | 0 | 3 | 2 |
Mitochondria | 123 | 29 | 4 | 28 | 27 | 85 | 193 | 128 | 209 | 165 | 214 | 192 | 2 | 1 | 0 | – | 7 | 8 | 163 | 110 | 102 | 85 | 165 | 217 |
Nucleus | 1 | 0 | 0 | 1 | 3 | 0 | 0 | 6 | 10 | 3 | 2 | 1 | 0 | 0 | 0 | – | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 |
Peroxisomes | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 1 | 0 | 1 | 1 | 1 | 3 |
SpindlePole | 8 | 3 | 1 | 12 | 25 | 20 | 25 | 24 | 16 | 22 | 13 | 23 | 5 | 5 | 7 | – | 15 | 9 | 8 | 6 | 5 | 5 | 13 | 15 |
Vac/Vac Membrane | 4 | 4 | 3 | 1 | 11 | 6 | 11 | 2 | 2 | 2 | 4 | 10 | 3 | 7 | 4 | – | 8 | 8 | 10 | 11 | 2 | 3 | 13 | 20 |
Unique Cell Count | 228 | 131 | 111 | 184 | 267 | 226 | 321 | 260 | 301 | 241 | 261 | 310 | 434 | 487 | 602 | 87 | 67 | 270 | 180 | 158 | 145 | 335 | 431 | |
Labelled Cell Count | 270 | 145 | 121 | 205 | 288 | 252 | 397 | 322 | 375 | 302 | 335 | 367 | 447 | 500 | 623 | 106 | 81 | 270 | 180 | 158 | 145 | 335 | 431 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 2.9 | 4.3 | 4.9 | 4.3 | 4.6 | 3.7 | 3.3 | 3.8 | 3.1 | 3.3 | 3.1 | 3.5 | 4.8 | 5.1 | 4.9 | – | 6.3 | 6.0 | 4.6 | 4.9 | 5.4 |
Std Deviation (1e-4) | 0.5 | 0.8 | 1.3 | 1.5 | 1.6 | 1.3 | 1.2 | 1.1 | 1.0 | 1.1 | 1.6 | 1.2 | 1.0 | 1.2 | 1.0 | – | 3.1 | 2.4 | 1.4 | 1.3 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.08 | -0.41 | -0.54 | -0.35 | -0.66 | -0.55 | -0.63 | -0.45 | -0.02 | 0.07 | -0.0 | – | 0.38 | 0.3 | -0.08 | 0.02 | 0.15 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.6566 | -0.9886 | -0.8932 | -0.924 | -0.618 | -0.9568 | -0.4312 | -0.3584 | -0.2834 | -0.7875 | -0.04 | -0.468 | -1.2113 | -1.2665 | -0.9949 | -0.4786 | -0.9796 | -1.089 |
Actin | 0.0506 | 0.0036 | 0.0161 | 0.0519 | 0.0735 | 0.0121 | 0.034 | 0.0013 | 0.0052 | 0.0033 | 0.0079 | 0.009 | 0.0123 | 0.0073 | 0.0408 | 0.0212 | 0.0073 | 0.0115 |
Bud | 0.0019 | 0.0012 | 0.0017 | 0.003 | 0.0008 | 0.0022 | 0.0006 | 0.0011 | 0.0017 | 0.0005 | 0.0008 | 0.0026 | 0.0023 | 0.0018 | 0.0017 | 0.0239 | 0.0026 | 0.002 |
Bud Neck | 0.003 | 0.0005 | 0.0016 | 0.0024 | 0.0013 | 0.0057 | 0.0091 | 0.0006 | 0.0047 | 0.0008 | 0.0006 | 0.0041 | 0.004 | 0.0011 | 0.0063 | 0.0046 | 0.0013 | 0.0126 |
Bud Periphery | 0.0024 | 0.0019 | 0.0057 | 0.0038 | 0.0016 | 0.0065 | 0.0015 | 0.0031 | 0.0036 | 0.0035 | 0.0025 | 0.006 | 0.0047 | 0.0029 | 0.0042 | 0.016 | 0.0071 | 0.0053 |
Bud Site | 0.0157 | 0.0145 | 0.0142 | 0.0093 | 0.0012 | 0.0016 | 0.0042 | 0.0079 | 0.0109 | 0.0008 | 0.0012 | 0.0008 | 0.0139 | 0.0185 | 0.0087 | 0.0205 | 0.0031 | 0.0007 |
Cell Periphery | 0.0011 | 0.0009 | 0.001 | 0.0005 | 0.0002 | 0.0005 | 0.001 | 0.0012 | 0.0008 | 0.0012 | 0.0004 | 0.0004 | 0.0013 | 0.0014 | 0.001 | 0.001 | 0.0025 | 0.0005 |
Cytoplasm | 0.0688 | 0.111 | 0.0667 | 0.0829 | 0.0671 | 0.1196 | 0.0525 | 0.1083 | 0.109 | 0.1205 | 0.0401 | 0.1566 | 0.0949 | 0.207 | 0.1771 | 0.173 | 0.2264 | 0.2351 |
Cytoplasmic Foci | 0.0955 | 0.0683 | 0.0816 | 0.0638 | 0.1434 | 0.0865 | 0.0542 | 0.0791 | 0.0518 | 0.1198 | 0.076 | 0.0942 | 0.117 | 0.1343 | 0.0932 | 0.1307 | 0.073 | 0.1098 |
Eisosomes | 0.0015 | 0.0009 | 0.0007 | 0.0004 | 0.0004 | 0.0004 | 0.0007 | 0.0006 | 0.0005 | 0.0015 | 0.0006 | 0.0002 | 0.0013 | 0.0011 | 0.0021 | 0.0006 | 0.0016 | 0.0004 |
Endoplasmic Reticulum | 0.0065 | 0.0038 | 0.0023 | 0.0018 | 0.0029 | 0.0019 | 0.0078 | 0.003 | 0.0022 | 0.0087 | 0.0012 | 0.003 | 0.0032 | 0.0073 | 0.0065 | 0.0039 | 0.009 | 0.0034 |
Endosome | 0.0467 | 0.0315 | 0.0296 | 0.0174 | 0.0659 | 0.0217 | 0.0493 | 0.0381 | 0.0394 | 0.0727 | 0.0508 | 0.0346 | 0.0561 | 0.0789 | 0.0687 | 0.0861 | 0.1201 | 0.0697 |
Golgi | 0.0111 | 0.0053 | 0.0052 | 0.006 | 0.0178 | 0.007 | 0.0073 | 0.0049 | 0.0035 | 0.0031 | 0.0069 | 0.0058 | 0.0136 | 0.0117 | 0.0132 | 0.016 | 0.0169 | 0.0107 |
Lipid Particles | 0.0239 | 0.0135 | 0.0116 | 0.0034 | 0.0314 | 0.0083 | 0.0191 | 0.0105 | 0.0062 | 0.0054 | 0.0091 | 0.0058 | 0.0395 | 0.016 | 0.017 | 0.014 | 0.0096 | 0.0068 |
Mitochondria | 0.0328 | 0.0188 | 0.0333 | 0.0496 | 0.0181 | 0.0356 | 0.0247 | 0.0261 | 0.0128 | 0.0815 | 0.0341 | 0.0319 | 0.0399 | 0.0168 | 0.0467 | 0.0611 | 0.0487 | 0.0472 |
None | 0.5646 | 0.6954 | 0.6839 | 0.6701 | 0.4528 | 0.6581 | 0.6835 | 0.6894 | 0.7279 | 0.5423 | 0.7384 | 0.6134 | 0.5582 | 0.4582 | 0.4777 | 0.3763 | 0.4444 | 0.4531 |
Nuclear Periphery | 0.0134 | 0.0014 | 0.0018 | 0.0013 | 0.0257 | 0.0025 | 0.0139 | 0.001 | 0.0024 | 0.0096 | 0.0021 | 0.0044 | 0.0007 | 0.0023 | 0.002 | 0.0013 | 0.0043 | 0.0012 |
Nucleolus | 0.0033 | 0.001 | 0.0014 | 0.0014 | 0.0009 | 0.0006 | 0.0014 | 0.001 | 0.0008 | 0.0005 | 0.0012 | 0.0006 | 0.0031 | 0.0009 | 0.0016 | 0.0036 | 0.0015 | 0.0005 |
Nucleus | 0.0046 | 0.0014 | 0.0017 | 0.004 | 0.0021 | 0.0014 | 0.0036 | 0.0021 | 0.0021 | 0.0035 | 0.0011 | 0.0031 | 0.0014 | 0.0016 | 0.0016 | 0.0084 | 0.003 | 0.0014 |
Peroxisomes | 0.0311 | 0.0152 | 0.0288 | 0.0135 | 0.0491 | 0.0136 | 0.0121 | 0.0093 | 0.0061 | 0.0038 | 0.0158 | 0.0145 | 0.0195 | 0.012 | 0.0186 | 0.0248 | 0.0043 | 0.0173 |
Punctate Nuclear | 0.0143 | 0.0044 | 0.0052 | 0.0098 | 0.0359 | 0.0105 | 0.0125 | 0.0022 | 0.0027 | 0.0023 | 0.0009 | 0.0042 | 0.0025 | 0.0043 | 0.0028 | 0.002 | 0.002 | 0.0022 |
Vacuole | 0.0052 | 0.0046 | 0.0045 | 0.0027 | 0.0054 | 0.0028 | 0.005 | 0.0078 | 0.0047 | 0.0123 | 0.0047 | 0.0038 | 0.0093 | 0.0118 | 0.0063 | 0.009 | 0.008 | 0.0056 |
Vacuole Periphery | 0.002 | 0.0011 | 0.0013 | 0.001 | 0.0025 | 0.0009 | 0.0023 | 0.0012 | 0.0012 | 0.0026 | 0.0036 | 0.0011 | 0.0013 | 0.003 | 0.0022 | 0.0022 | 0.0034 | 0.0028 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.1791 | 8.3503 | 13.1671 | 13.5322 | 12.8679 | 22.1726 | 14.7752 | 12.8215 | 14.129 | 17.009 |
Translational Efficiency | 0.3074 | 0.6652 | 0.3118 | 0.429 | 0.4681 | 0.2411 | 0.3668 | 0.4872 | 0.4213 | 0.3696 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|