Standard name
Human Ortholog
Description Serine-rich protein of unknown function; predicted to be palmitoylated; overproduction suppresses growth inhibition caused by exposure to immunosuppressant leflunomide; MLF3 has a paralog, VHS2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.15 0.19 0.34 0.34 0.22 0.26 0.19 0.22 0.28 0.24 0.24 0.31 0.18 0.2 0.17 0.21 0.2 0.28 0.1 0 0.21 0.06 0.06 0.1
Cytoplasm 0.92 0.99 0.95 0.92 0.88 0.84 0.83 0.76 0.7 0.73 0.69 0.66 1.0 0.98 0.99 0.94 0.97 0.97 0.76 0.85 0.61 0.86 0.84 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0.07 0.19 0.26 0.28 0.29 0.29 0.36 0.33 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 1 3 2 7 4 5 0 0 0 0 0 0 0 1 4 4 1 2 3
Bud 5 6 2 0 3 5 9 15 9 23 11 14 0 0 0 1 1 0 3 8 6 0 10 15
Bud Neck 1 0 1 0 1 0 0 2 1 0 0 1 0 0 0 0 0 0 1 1 2 0 2 3
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 28 36 95 50 43 72 70 94 94 108 58 81 52 46 57 93 67 91 19 6 58 10 18 26
Cytoplasm 177 183 263 135 169 229 300 330 232 327 167 172 282 227 341 406 318 311 150 155 169 134 249 211
Endoplasmic Reticulum 0 0 3 4 1 4 1 6 3 2 3 4 1 0 3 11 4 6 0 0 6 1 0 3
Endosome 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 1 1 0 3 0 1 1
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1
Mitochondria 24 1 2 5 14 52 93 122 95 131 87 85 0 0 0 3 3 1 7 1 15 0 0 1
Nucleus 0 0 1 0 1 1 2 3 1 0 1 1 0 1 1 6 4 3 0 0 0 0 3 0
Nuclear Periphery 0 0 0 0 0 1 1 4 1 2 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 1 0 3 2 1 0 0 0 0 1 0 0 0 5 1 4 0 2 1
Vac/Vac Membrane 0 0 2 0 0 1 4 8 4 9 2 1 0 2 1 20 7 5 2 1 1 0 1 0
Unique Cell Count 192 185 278 146 192 273 361 435 330 447 242 260 283 231 345 433 327 322 197 183 277 157 299 272
Labelled Cell Count 235 227 370 196 235 367 484 590 449 607 334 360 336 276 404 541 404 419 197 183 277 157 299 272


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 6.5 6.5 6.1 5.6 5.1 5.2 5.3 5.3 5.3 5.0 5.5 8.1 7.8 7.4 9.7 9.0 9.2 7.7 7.8 8.5
Std Deviation (1e-4) 1.8 2.4 2.2 2.5 2.2 2.1 2.2 1.7 1.7 1.6 1.8 1.6 2.1 2.1 2.2 1.4 1.5 1.4 2.1 2.1 2.3
Intensity Change (Log2) – – – -0.1 -0.22 -0.34 -0.32 -0.28 -0.29 -0.28 -0.36 -0.23 0.33 0.26 0.2 0.58 0.47 0.5 0.25 0.28 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 2.3 1.9 3.2 2.8 3.2 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -2.8 -2.0 -4.2 -3.7 -1.5 -2.9 -2.5 -0.7 -4.3 -3.6 -5.1 -3.7 -3.8 -1.6
Cytoplasm -0.9 -2.6 -4.1 -4.5 -6.5 -7.7 -7.2 -7.7 -8.4 3.6 2.2 3.1 -0.5 1.7 1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.9 7.2 8.8 9.4 9.4 9.7 10.6 10.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.9 0 0