Standard name
Human Ortholog
Description Putative mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency; localizes to vacuole in response to H2O2

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.1 0.09 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.07 0.1 0 0 0 0 0.05 0 0 0
Golgi 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0.1 0.13 0.11 0.23 0.42 0.45 0.06 0.07 0 0.11 0.08 0.12
Mitochondria 0.91 0.85 0.98 0.96 0.88 0.93 0.92 0.89 0.94 0.94 0.96 0.97 0.87 0.9 0.82 0.13 0.06 0.09 0.8 0.76 0.83 0.66 0.66 0.66
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0.05 0.07 0.13 0.15 0.09 0.15 0.1 0.1 0.13 0 0 0 0 0.08 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.07 0.08 0.1 0.1 0.07 0.09 0.06 0.07 0 0 0 0.07 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.13 0.22 0.16 0 0 0 0.07 0.09 0.08
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.17 0.06 0 0 0 0 0.05 0.05 0 0 0 0.09 0 0.1 0.23 0.06 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 4 1 0 0 1 0 1 1 1 1 1 0 0 0 5 1 3 6 21 2 9 13 11
Bud 7 6 31 9 16 24 15 25 19 14 5 14 2 4 3 7 3 3 2 4 1 0 3 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2
Bud Site 0 0 6 1 1 6 5 2 4 3 10 4 0 0 0 0 0 0
Cell Periphery 23 30 18 9 4 3 0 2 6 3 3 10 9 6 11 16 5 8 0 0 0 0 0 2
Cytoplasm 3 14 7 10 12 9 11 18 11 11 8 6 7 9 8 14 3 4 1 2 1 0 1 0
Endoplasmic Reticulum 5 5 2 4 3 2 2 5 5 2 8 6 1 6 2 7 2 0 1 0 2 0 0 0
Endosome 5 19 2 1 30 3 1 9 2 4 0 0 10 13 23 18 2 3 17 27 26 5 11 8
Golgi 25 30 0 0 4 3 1 2 0 0 4 0 26 44 38 41 21 38 27 47 17 17 25 37
Mitochondria 422 554 549 312 512 589 823 797 772 896 554 650 218 294 274 23 3 8 383 527 482 106 205 212
Nucleus 5 1 1 0 3 1 4 10 2 3 1 2 0 0 0 3 0 1 0 0 0 1 1 0
Nuclear Periphery 22 20 24 15 40 83 130 77 125 96 59 84 7 10 9 1 4 0 2 4 2 0 3 1
Nucleolus 10 13 23 9 39 52 87 89 59 86 37 44 3 8 6 12 0 0 1 2 0 0 4 2
Peroxisomes 4 23 5 1 22 6 10 7 6 7 3 3 11 19 32 24 11 14 11 12 25 10 26 23
SpindlePole 2 2 2 0 14 13 5 10 4 8 3 2 3 2 4 14 2 3 0 6 1 1 3 0
Vac/Vac Membrane 76 110 36 14 24 18 25 41 37 28 21 12 23 13 33 41 3 13 18 25 8 2 10 13
Unique Cell Count 463 650 560 326 580 634 891 900 817 956 577 671 250 328 334 180 50 85 477 693 579 161 314 319
Labelled Cell Count 612 831 707 385 724 813 1119 1095 1053 1162 717 838 320 428 443 226 60 98 477 693 579 161 314 319


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.1 9.0 4.9 6.2 7.0 6.2 6.4 6.8 6.6 6.8 6.9 6.9 9.6 9.4 9.5 9.5 9.4 9.5 9.9 10.9 11.3
Std Deviation (1e-4) 1.4 1.8 1.0 1.6 1.4 1.5 1.6 1.7 1.8 1.9 2.0 1.9 1.6 1.7 1.7 2.5 2.4 2.7 2.2 2.3 2.5
Intensity Change (Log2) 0.35 0.51 0.33 0.37 0.47 0.42 0.47 0.5 0.5 0.97 0.94 0.95 0.96 0.93 0.96 1.01 1.16 1.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -1.9 -2.4 -1.4 -4.1 -2.7 -3.1 -4.5 -4.5 -3.2 -3.1 -3.2 -3.5 -0.9 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 -0.2 0 0 0 0 0.9 0 0 0 0 0 0 0
Cell Periphery -0.4 -3.1 -3.6 -5.4 -4.8 -3.5 -4.7 -3.4 -2.0 0.3 -1.2 0.1 3.2 0 0
Cytoplasm 1.9 1.1 0.3 0 1.1 0.2 -0.2 0.2 -0.6 0 1.6 1.3 4.6 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 4.9 0 0 0 0 0 0 0 0 4.0 5.7 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 7.8 8.9 8.2 11.6 0 16.3
Mitochondria -2.0 -6.5 -4.2 -4.6 -6.6 -3.3 -3.8 -2.0 -1.3 -6.4 -5.5 -8.6 -23.8 -21.3 -22.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.2 1.9 5.3 6.2 3.1 6.5 4.0 3.8 5.1 -1.0 -0.9 -1.2 -2.4 0 0
Nucleolus -1.0 1.9 2.9 4.0 4.0 2.4 3.6 1.7 1.9 -2.2 -1.3 -1.9 1.4 0 0
Peroxisomes 0 3.2 0.1 0.4 0 0 0 0 0 0 4.3 6.3 7.5 0 0
SpindlePole 0 3.0 2.6 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.3 -1.7 -3.0 -3.3 -1.6 -1.5 -3.3 -2.2 -4.2 1.4 -1.6 1.9 6.2 0 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.3259 9.1226 8.7242 8.1386 6.7299 8.8464 7.8584 8.0673 7.5658 6.3154 6.9752 7.7345 10.9826 9.4886 9.8097 8.8017 10.2022 9.8251
Actin 0.0269 0.015 0.0042 0.0013 0.0025 0.0004 0.0086 0.0031 0.0021 0.001 0.0151 0.0009 0.001 0.0015 0.0005 0.0004 0.0005 0.0021
Bud 0.0007 0.0008 0.0008 0.0003 0.0001 0 0.0002 0.0002 0.0001 0.0001 0.0002 0 0.0001 0.0006 0 0.0005 0.0001 0
Bud Neck 0.0005 0.0003 0.0001 0.0004 0 0 0.0004 0 0.0001 0 0.0026 0 0 0.0001 0 0.0002 0 0
Bud Periphery 0.0012 0.0009 0.0016 0.0007 0.0001 0 0.0004 0.0002 0.0002 0.0003 0.0004 0 0.0002 0.0011 0 0.0015 0.0001 0
Bud Site 0.0088 0.0072 0.0006 0.0007 0.0001 0 0.0011 0.0004 0.0001 0 0.0006 0 0.0001 0.0009 0 0.0009 0 0
Cell Periphery 0.0011 0.0003 0.0003 0.0004 0.0001 0 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0002 0 0.0016 0 0
Cytoplasm 0.0012 0.0002 0.0003 0.0001 0 0 0.0007 0 0.0003 0 0.001 0 0 0.0001 0 0.0001 0 0
Cytoplasmic Foci 0.012 0.0016 0.004 0.0089 0.0008 0.0006 0.0076 0.0046 0.0048 0.0031 0.0107 0.0003 0.0005 0.0013 0.0002 0.0036 0.0004 0.0009
Eisosomes 0.0004 0.0003 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0007 0.0002 0.0006 0.0002 0 0 0.0009 0 0.0013 0.0001 0.0008 0.0001 0.0001 0.0001 0 0 0 0.0001
Endosome 0.0073 0.012 0.0088 0.0171 0.0036 0.0003 0.0171 0.0048 0.011 0.0039 0.0175 0.0022 0.0043 0.0094 0.0009 0.0144 0.0015 0.0043
Golgi 0.0095 0.0112 0.0088 0.0066 0.0199 0.0023 0.0224 0.0127 0.0177 0.0261 0.0261 0.0147 0.0121 0.0186 0.0052 0.0324 0.0039 0.0161
Lipid Particles 0.0366 0.0113 0.0145 0.0307 0.0018 0.0051 0.0237 0.0041 0.0025 0.0289 0.0233 0.0012 0.0043 0.0016 0.0006 0.0022 0.0001 0.0009
Mitochondria 0.8207 0.9179 0.9308 0.8922 0.9672 0.9836 0.8969 0.9581 0.9466 0.9223 0.8733 0.9781 0.9709 0.9495 0.9876 0.93 0.9914 0.9705
None 0.0026 0.0001 0.0002 0.0002 0 0 0.0009 0 0.0004 0.0001 0.002 0 0 0 0 0 0 0
Nuclear Periphery 0.0036 0.0011 0.0006 0.0008 0 0 0.0006 0 0.0017 0.0002 0.0023 0 0.0001 0.0001 0 0 0 0
Nucleolus 0.0188 0.0002 0.001 0.0021 0.0001 0 0.001 0.0007 0.0001 0.0002 0.0009 0 0 0.0001 0 0.0004 0 0
Nucleus 0.0016 0.0002 0.0003 0.0007 0 0 0.001 0 0.0002 0.0001 0.0009 0 0 0.0001 0 0.0001 0 0
Peroxisomes 0.0278 0.0062 0.0057 0.0089 0.002 0.0066 0.0104 0.0094 0.0046 0.0087 0.0112 0.0011 0.0029 0.007 0.0017 0.0088 0.0016 0.003
Punctate Nuclear 0.0023 0 0.0001 0.0005 0 0 0.0008 0.0001 0.0016 0 0.0046 0 0 0 0 0 0 0
Vacuole 0.008 0.0036 0.008 0.014 0.0003 0.0001 0.0021 0.0004 0.0012 0.0008 0.0018 0.0002 0.0006 0.0036 0.0007 0.0012 0.0001 0.0003
Vacuole Periphery 0.0077 0.0096 0.0086 0.0129 0.0011 0.0009 0.0028 0.0009 0.0032 0.0039 0.0039 0.001 0.0026 0.004 0.0023 0.0015 0.0002 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.2647 15.9829 22.0258 36.1416 21.5254 25.5276 39.8237 39.7332 39.4474 43.7253
Translational Efficiency 2.6144 3.1773 2.3169 1.8135 2.7527 3.3636 1.8023 2.0387 2.2424 1.8655

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2283 1482 541 595 1611 1826 2294 1614 3894 3308 2835 2209

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 865.35 980.23 1318.86 1330.80 822.88 991.27 1249.77 1248.43 847.78 986.32 1262.95 1270.62
Standard Deviation 135.43 174.27 214.20 228.22 139.55 163.53 204.21 218.64 138.74 168.52 207.94 224.26
Intensity Change Log 2 0.179837 0.607936 0.620938 0.268596 0.602909 0.601361 0.223782 0.605488 0.611429

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000641 0.002112 0.005363 0.007124 0.001500 0.001502 0.002436 0.002942 0.000996 0.001776 0.002995 0.004068
Bud Neck 0.000585 0.001205 0.006526 0.001810 0.002049 0.001312 0.000700 0.000680 0.001191 0.001264 0.001812 0.000984
Bud Site 0.002611 0.001786 0.005356 0.003872 0.005092 0.002788 0.001504 0.003008 0.003637 0.002339 0.002239 0.003241
Cell Periphery 0.000250 0.000150 0.000243 0.000294 0.001453 0.000225 0.000107 0.000316 0.000748 0.000191 0.000133 0.000310
Cytoplasm 0.000850 0.000352 0.000729 0.000508 0.000669 0.000543 0.000300 0.000473 0.000775 0.000458 0.000382 0.000482
Cytoplasmic Foci 0.005898 0.014812 0.041842 0.015691 0.004498 0.016150 0.005961 0.008300 0.005319 0.015551 0.012808 0.010291
Eisosomes 0.000032 0.000062 0.000171 0.000177 0.000059 0.000064 0.000095 0.000104 0.000043 0.000063 0.000110 0.000123
Endoplasmic Reticulum 0.000129 0.000203 0.000617 0.000525 0.000275 0.000163 0.000138 0.000322 0.000189 0.000181 0.000230 0.000377
Endosome 0.015857 0.020127 0.026103 0.012949 0.008412 0.031459 0.004887 0.007802 0.012777 0.026382 0.008936 0.009189
Golgi 0.011728 0.025642 0.049294 0.053234 0.008577 0.024080 0.014510 0.032818 0.010424 0.024780 0.021148 0.038317
Lipid Particles 0.002654 0.003384 0.006488 0.001616 0.003355 0.005744 0.001607 0.001009 0.002944 0.004687 0.002539 0.001173
Mitochondria 0.941014* 0.894162* 0.755570* 0.854715* 0.943937* 0.882490* 0.946941* 0.917160* 0.942224* 0.887719* 0.910422* 0.900340*
Mitotic Spindle 0.000614 0.003395 0.023407 0.011270 0.000753 0.002409 0.004051 0.002724 0.000671 0.002851 0.007745 0.005026
None 0.000253 0.000336 0.001242 0.000897 0.000460 0.000398 0.000501 0.000581 0.000338 0.000370 0.000642 0.000666
Nuclear Periphery 0.000050 0.000421 0.003847 0.001479 0.000055 0.000208 0.000284 0.000300 0.000052 0.000303 0.000964 0.000617
Nuclear Periphery Foci 0.000104 0.000348 0.002327 0.000925 0.000091 0.000256 0.000180 0.000165 0.000099 0.000297 0.000590 0.000370
Nucleolus 0.000130 0.000331 0.001764 0.000119 0.000318 0.000260 0.000125 0.000084 0.000208 0.000292 0.000438 0.000094
Nucleus 0.000504 0.000673 0.000443 0.000063 0.000308 0.000237 0.000034 0.000031 0.000423 0.000433 0.000112 0.000040
Peroxisomes 0.008506 0.015052 0.023826 0.008761 0.012055 0.015245 0.007092 0.006593 0.009975 0.015159 0.010285 0.007177
Vacuole 0.002733 0.006627 0.021941 0.008996 0.002437 0.007896 0.003917 0.005778 0.002610 0.007328 0.007357 0.006645
Vacuole Periphery 0.004857 0.008817 0.022900 0.014976 0.003648 0.006571 0.004629 0.008809 0.004357 0.007577 0.008115 0.010470

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.70 -4.02 -3.82 -2.82 -0.86 -0.01 -2.04 -2.41 -2.35 -0.82 -2.33 -5.20 -5.04 -3.46 -1.55
Bud Neck -1.84 -6.12 -3.41 -1.36 4.65 1.15 2.33 2.27 1.87 -0.06 -0.22 -1.95 0.64 1.02 3.30
Bud Site 1.00 -2.60 -1.16 -2.12 1.25 1.85 3.06 1.50 -0.30 -1.84 1.83 2.01 0.41 -1.33 -1.50
Cell Periphery 2.35 0.12 -0.80 -3.05 -0.79 3.96 4.35 3.60 -1.13 -2.75 4.22 4.66 3.13 -2.04 -3.04
Cytoplasm 1.41 0.36 0.97 -1.87 1.64 0.46 1.42 0.71 0.50 -1.70 1.35 1.67 1.21 -0.30 -1.24
Cytoplasmic Foci -6.58 -9.52 -4.55 -0.37 6.11 -8.37 -1.90 -3.54 4.96 -2.21 -10.43 -7.81 -5.16 4.17 2.02
Eisosomes -4.97 -8.13 -4.04 -3.17 -0.13 -0.69 -3.49 -3.57 -3.22 -0.59 -4.28 -8.06 -6.20 -4.56 -0.91
Endoplasmic Reticulum -2.14 -4.34 -4.09 -3.20 0.65 2.52 2.09 -0.67 -2.56 -2.34 0.30 -0.81 -3.48 -3.67 -2.13
Endosome -2.03 -3.18 1.36 3.11 3.91 -9.89 3.26 0.47 10.38 -3.05 -8.29 3.36 3.10 10.95 -0.25
Golgi -6.31 -8.05 -7.67 -4.86 -0.57 -7.48 -3.60 -8.88 -2.84 -6.52 -9.52 -6.94 -11.39 -5.05 -6.35
Lipid Particles -0.87 -3.41 1.67 2.70 4.86 -2.10 2.31 3.05 4.95 1.35 -2.42 0.76 3.51 5.70 3.47
Mitochondria 6.98 13.01 7.88 3.34 -5.76 8.76 -0.62 4.17 -4.63 5.16 11.24 6.69 7.91 -2.04 1.68
Mitotic Spindle -3.36 -6.07 -3.93 -2.79 2.65 -2.90 -3.46 -2.82 -0.39 1.21 -4.57 -6.77 -4.95 -2.22 2.04
None -2.04 -8.91 -9.41 -9.34 2.89 0.45 -0.30 -0.92 -2.45 -2.30 -0.44 -4.89 -5.27 -6.32 -0.64
Nuclear Periphery -2.49 -3.72 -2.34 -1.68 1.99 -4.20 -3.78 -1.74 -0.64 -0.10 -3.61 -4.51 -2.91 -1.52 1.24
Nuclear Periphery Foci -2.87 -4.75 -1.76 -1.22 2.14 -5.01 -1.89 -1.92 1.91 0.27 -4.54 -4.93 -2.09 -0.54 1.38
Nucleolus -1.57 -2.59 0.15 1.90 2.62 0.61 1.08 1.21 1.68 0.84 -0.18 -0.96 1.26 2.55 2.73
Nucleus -0.26 0.14 1.14 1.14 2.18 0.66 1.71 1.72 1.61 0.26 0.08 1.42 1.73 1.57 2.11
Peroxisomes -2.94 -4.21 -0.12 2.36 3.84 -1.40 2.56 2.72 4.71 0.35 -3.39 -0.22 2.11 5.28 2.26
Vacuole -5.02 -7.20 -4.11 -1.46 4.27 -6.33 -2.38 -3.43 1.72 -1.89 -8.05 -6.81 -4.99 0.66 0.66
Vacuole Periphery -3.69 -7.71 -5.48 -3.11 2.76 -3.63 -1.45 -5.24 -2.19 -4.51 -4.87 -5.43 -7.05 -3.11 -2.47
Standard name
Human Ortholog
Description Putative mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency; localizes to vacuole in response to H2O2
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Sws2

Sws2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sws2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available