Standard name | |
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Human Ortholog | |
Description | Catalytic subunit of the DNA polymerase I alpha-primase complex; required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 7 | 9 | 4 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 0 | 4 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1 | 0 | 1 | 1 | 0 | 2 | 12 | 9 | 20 | 23 | 10 | 25 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 4 | 4 | 10 | 18 | 22 | 20 | 47 | 20 | 24 | 14 | 16 | 11 | 28 | 32 | 11 | 8 | 12 | 0 | 1 | 0 | 0 | 2 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 3 | 1 | 1 | 1 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 2 | 17 | 50 | 97 | 84 | 118 | 169 | 128 | 238 | 340 | 165 | 275 | 4 | 4 | 35 | 3 | 1 | 3 | 1 | 4 | 0 | 0 | 2 | 4 |
Nucleus | 137 | 231 | 171 | 154 | 302 | 301 | 365 | 327 | 351 | 476 | 195 | 337 | 162 | 192 | 192 | 67 | 44 | 41 | 126 | 228 | 156 | 127 | 163 | 182 |
Nuclear Periphery | 5 | 18 | 10 | 2 | 46 | 34 | 47 | 23 | 31 | 40 | 18 | 40 | 3 | 1 | 4 | 8 | 6 | 3 | 10 | 4 | 4 | 2 | 1 | 0 |
Nucleolus | 10 | 12 | 7 | 9 | 15 | 18 | 21 | 22 | 43 | 60 | 6 | 40 | 7 | 12 | 24 | 0 | 0 | 0 | 4 | 9 | 5 | 1 | 2 | 7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 3 | 2 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 4 | 2 |
Vac/Vac Membrane | 6 | 30 | 6 | 5 | 25 | 33 | 13 | 39 | 10 | 12 | 5 | 13 | 3 | 4 | 8 | 10 | 10 | 6 | 4 | 12 | 15 | 4 | 31 | 37 |
Unique Cell Count | 146 | 263 | 189 | 187 | 377 | 391 | 466 | 461 | 478 | 652 | 281 | 470 | 179 | 232 | 251 | 88 | 64 | 61 | 150 | 273 | 187 | 142 | 218 | 248 |
Labelled Cell Count | 164 | 312 | 249 | 281 | 491 | 529 | 651 | 600 | 724 | 987 | 417 | 750 | 190 | 246 | 300 | 101 | 70 | 68 | 150 | 273 | 187 | 142 | 218 | 248 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.4 | 5.7 | 4.3 | 4.4 | 4.7 | 4.6 | 4.3 | 5.4 | 4.0 | 3.9 | 4.1 | 3.6 | 6.4 | 6.4 | 5.4 | 7.8 | 8.9 | 8.9 | 6.0 | 5.4 | 6.0 |
Std Deviation (1e-4) | 1.3 | 1.1 | 0.8 | 1.7 | 1.4 | 1.5 | 1.2 | 3.2 | 1.5 | 1.2 | 1.6 | 1.0 | 1.5 | 1.7 | 1.4 | 1.9 | 2.4 | 2.3 | 1.6 | 1.5 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.13 | 0.08 | -0.03 | 0.32 | -0.1 | -0.13 | -0.07 | -0.26 | 0.57 | 0.56 | 0.31 | 0.85 | 1.05 | 1.05 | 0.46 | 0.32 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.7 | 1.5 | 1.9 | 1.3 | 3.5 | 1.3 | 1.1 | 1.6 | 0.9 | 2.0 | 3.8 | 4.0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0.3 | 0 | 0 | 0 | 0 | -6.6 | -7.5 | -3.3 | -4.5 | -4.3 | -3.6 |
Nucleus | -2.3 | -3.1 | -3.9 | -3.7 | -5.3 | -4.8 | -5.0 | -5.4 | -5.2 | 0 | -2.3 | -3.8 | -3.2 | -4.2 | -4.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | -0.2 | 0 | 0 | 0 | 0 | -1.9 | 0 | -2.2 | 1.2 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0.6 | 0 | 0 | 0 | 0 | 0.1 | 0.7 | 2.4 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.3 | 1.7 | 2.4 | -0.3 | 2.4 | 0 | 0 | 0 | -0.3 | 0 | 0 | 0 | 2.7 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.652 | 5.9307 | 5.7789 | 4.5722 | 4.5844 | 4.9839 | 4.5142 | 5.7837 | 5.9757 | 5.2017 | 4.4622 | 4.6344 | 3.0933 | 3.4619 | 3.4957 | 3.7453 | 2.8264 | 3.1764 |
Actin | 0.0403 | 0.0018 | 0.002 | 0.0011 | 0.0156 | 0.0006 | 0.0066 | 0 | 0.0009 | 0.0001 | 0.0025 | 0.0007 | 0 | 0 | 0.0002 | 0.0001 | 0.0015 | 0 |
Bud | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0012 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 |
Bud Neck | 0.0027 | 0.0003 | 0.0002 | 0.0002 | 0.0008 | 0.0023 | 0.0014 | 0 | 0 | 0 | 0.0061 | 0.0007 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0004 |
Bud Periphery | 0.0027 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0011 | 0.003 | 0 | 0 | 0 | 0.0004 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0005 | 0.0001 |
Bud Site | 0.0034 | 0.0171 | 0.0012 | 0.0004 | 0.0003 | 0.0003 | 0.0039 | 0 | 0 | 0 | 0.0033 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0004 | 0 |
Cell Periphery | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0009 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0035 | 0.0002 | 0.0008 | 0.0005 | 0.0005 | 0.0006 | 0.0121 | 0 | 0.0002 | 0.0001 | 0.0023 | 0.0002 | 0 | 0.0007 | 0.0004 | 0.001 | 0.0003 | 0.0001 |
Cytoplasmic Foci | 0.0078 | 0.0006 | 0.007 | 0.0038 | 0.0012 | 0.002 | 0.0235 | 0 | 0.0015 | 0 | 0.007 | 0.0015 | 0 | 0 | 0.0121 | 0 | 0.0055 | 0 |
Eisosomes | 0.0005 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0042 | 0 | 0.0031 | 0.0017 | 0.0015 | 0.0015 | 0.0045 | 0 | 0.0002 | 0.0001 | 0.0043 | 0.0003 | 0 | 0.0001 | 0.0004 | 0.0005 | 0.0011 | 0.0001 |
Endosome | 0.0156 | 0.0001 | 0.0055 | 0.0063 | 0.014 | 0.0033 | 0.0177 | 0 | 0.0034 | 0 | 0.0318 | 0.0033 | 0 | 0.0002 | 0.0013 | 0.0001 | 0.0053 | 0 |
Golgi | 0.006 | 0.0002 | 0.0028 | 0.0024 | 0.0214 | 0.0005 | 0.0028 | 0 | 0.0009 | 0 | 0.006 | 0.0013 | 0 | 0 | 0.0003 | 0 | 0.007 | 0 |
Lipid Particles | 0.0196 | 0 | 0.0136 | 0.0339 | 0.0054 | 0.0051 | 0.0106 | 0 | 0.0026 | 0 | 0.0206 | 0.0043 | 0 | 0 | 0.0064 | 0.0001 | 0.0085 | 0 |
Mitochondria | 0.0282 | 0.0124 | 0.0255 | 0.0035 | 0.0109 | 0.0111 | 0.0141 | 0.0003 | 0.0004 | 0.0003 | 0.002 | 0.0021 | 0.0001 | 0.0015 | 0.0006 | 0.0042 | 0.0492 | 0.0002 |
None | 0.0035 | 0 | 0.001 | 0.0002 | 0.0002 | 0.0002 | 0.0049 | 0 | 0.0002 | 0 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0014 | 0.0001 | 0 |
Nuclear Periphery | 0.0613 | 0.0105 | 0.0241 | 0.4004 | 0.3593 | 0.0568 | 0.0356 | 0.0128 | 0.014 | 0.1382 | 0.2076 | 0.0889 | 0.0015 | 0.0214 | 0.0582 | 0.3194 | 0.5428 | 0.0463 |
Nucleolus | 0.0226 | 0.0166 | 0.0141 | 0.0039 | 0.0071 | 0.0263 | 0.0154 | 0.0082 | 0.0055 | 0.0013 | 0.0053 | 0.0106 | 0.0061 | 0.008 | 0.0063 | 0.0004 | 0.0037 | 0.0192 |
Nucleus | 0.7384 | 0.9386 | 0.884 | 0.5267 | 0.5504 | 0.8773 | 0.7946 | 0.9778 | 0.9669 | 0.8586 | 0.6828 | 0.8768 | 0.9922 | 0.9656 | 0.9034 | 0.6635 | 0.3545 | 0.9325 |
Peroxisomes | 0.003 | 0.0002 | 0.0029 | 0.0025 | 0.0004 | 0.0005 | 0.0035 | 0 | 0.0007 | 0 | 0.0011 | 0.0028 | 0 | 0 | 0.0005 | 0 | 0.0074 | 0 |
Punctate Nuclear | 0.0189 | 0.0003 | 0.0082 | 0.0031 | 0.0007 | 0.0017 | 0.0351 | 0.0001 | 0.0021 | 0.0001 | 0.0049 | 0.0009 | 0 | 0.0003 | 0.0078 | 0.0004 | 0.0008 | 0.0002 |
Vacuole | 0.0089 | 0.0002 | 0.0012 | 0.001 | 0.0014 | 0.0038 | 0.0045 | 0.0001 | 0.0001 | 0.0001 | 0.0061 | 0.0008 | 0 | 0.0007 | 0.0007 | 0.0002 | 0.0013 | 0.0003 |
Vacuole Periphery | 0.0072 | 0.0003 | 0.002 | 0.0081 | 0.008 | 0.0044 | 0.0039 | 0.0004 | 0.0003 | 0.0011 | 0.0044 | 0.0038 | 0 | 0.0009 | 0.0008 | 0.0088 | 0.0096 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 92.5137 | 22.1582 | 9.1346 | 27.0266 | 70.2808 | 67.0635 | 18.557 | 12.7538 | 19.9323 | 75.8339 |
Translational Efficiency | 1.1358 | 0.8872 | 0.8148 | 0.7246 | 0.8669 | 1.3695 | 0.901 | 0.6976 | 0.6019 | 0.7012 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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