Standard name
Human Ortholog
Description Cytochrome b5; involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.06 0.05 0 – 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.06 – 0 0 0 0.05 0 0.07 0.11 0.11 0.1 0.07 0.09 0 0 0 0 0 0
Cytoplasm 0.2 0.22 0.23 0.23 0.21 0.3 – 0.37 0.42 0.43 0.44 0.39 0.13 0.2 0.24 0.64 0.67 0.64 0.07 0 0 0 0 0
Endoplasmic Reticulum 0.68 0.71 0.52 0.68 0.48 0.54 – 0.47 0.44 0.4 0.42 0.42 0.82 0.73 0.69 0 0 0 0.79 0.87 0.82 0.81 0.86 0.79
Endosome 0 0 0.07 0.06 0.12 0.06 – 0 0 0 0 0 0.11 0.1 0.07 0.06 0.07 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.13 0.13 0 0.08 – 0.06 0.09 0.07 0.12 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.16 0.13 0 0.2 0.1 – 0.11 0.09 0.13 0.08 0.09 0 0 0 0.23 0.13 0.14 0 0 0 0.06 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 12 10 12 16 10 – 21 9 10 5 17 14 14 5 11 8 9 8 5 5 3 8 3
Bud 1 1 13 4 2 7 – 9 5 8 12 13 2 2 2 1 0 1 5 2 2 1 2 5
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 4 4 1 0 2 2
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 5 0 8 11 24 – 18 14 12 19 23 26 44 56 25 12 17 0 0 0 0 1 0
Cytoplasm 77 77 76 46 69 113 – 189 153 140 164 207 52 84 123 165 113 123 29 8 14 10 12 18
Endoplasmic Reticulum 264 251 174 134 159 201 – 238 157 128 156 220 316 303 362 5 1 4 350 348 347 210 343 342
Endosome 15 7 23 11 38 22 – 7 12 5 2 13 41 41 37 16 11 15 11 6 12 2 5 9
Golgi 0 3 0 1 1 1 – 1 0 0 0 0 4 1 2 2 2 3 1 4 1 0 2 7
Mitochondria 1 1 43 26 8 29 – 29 32 21 44 73 0 0 0 4 3 4 3 2 10 3 5 6
Nucleus 1 0 1 0 0 0 – 6 1 1 1 0 1 1 2 1 4 6 0 0 0 1 1 2
Nuclear Periphery 0 3 4 5 4 2 – 11 8 4 7 10 5 6 4 1 0 1 9 7 8 5 2 2
Nucleolus 0 0 0 0 0 0 – 2 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 4 1 2 0 – 0 0 0 0 0 2 2 6 1 0 2 2 3 2 0 0 0
Vac/Vac Membrane 64 57 44 1 67 39 – 55 33 42 31 47 5 5 15 59 22 26 13 5 12 16 11 25
Unique Cell Count 387 356 335 196 330 372 506 360 323 374 530 387 417 521 259 168 192 444 400 425 259 401 432
Labelled Cell Count 437 417 392 249 378 448 586 425 371 442 623 468 503 614 292 176 211 444 400 425 259 401 432


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 11.1 6.8 9.2 10.0 9.1 – 9.3 9.0 9.2 9.1 8.9 11.1 10.7 10.2 9.4 9.3 8.8 11.6 12.4 12.1
Std Deviation (1e-4) 1.5 1.4 1.3 1.8 1.6 1.5 – 1.7 1.8 1.7 1.8 1.9 1.8 1.6 1.5 1.7 1.7 1.6 2.1 2.2 2.4
Intensity Change (Log2) – – – 0.44 0.56 0.42 – 0.45 0.42 0.44 0.42 0.39 0.72 0.66 0.59 0.48 0.46 0.37 0.78 0.87 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.8 1.2 -0.2 – 0.9 -0.4 0.1 -1.5 0.2 0.5 0.3 -2.2 0.8 1.0 1.0
Bud -1.2 -2.8 -1.6 – -1.9 -2.1 -1.0 -0.5 -1.2 -3.2 -3.3 -3.8 -2.8 0 -2.3
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 3.4 4.7 – 3.5 3.6 3.6 4.2 3.9 4.8 6.1 6.2 5.8 0 5.5
Cytoplasm 0.2 -0.6 2.3 – 4.5 5.6 5.6 5.9 5.0 -3.2 -0.8 0.3 10.1 9.7 9.4
Endoplasmic Reticulum 3.7 -1.0 0.6 – -1.4 -2.2 -3.2 -2.7 -3.0 8.5 5.9 5.2 -13.2 -11.4 -11.6
Endosome -0.6 2.1 -0.5 – -4.2 -2.1 -3.4 -4.6 -3.2 1.8 1.4 0.1 -0.3 -0.1 0.4
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 -5.0 -2.2 – -3.6 -1.7 -2.7 -0.4 0.4 -7.3 -7.5 -8.4 -5.1 -4.1 -4.2
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 1.1 1.0 0 0.7 0.8 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -5.0 2.5 -1.1 – -1.0 -1.7 0 -2.1 -2.0 -6.3 -6.6 -5.8 3.1 0 0.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.5258 10.0755 9.4197 8.865 8.4197 9.6339 8.8611 10.1018 9.558 8.6287 7.6655 9.6387 10.8255 12.7982 12.0196 12.1624 10.7103 12.1355
Actin 0.0017 0.0032 0.0121 0.0004 0.0364 0.0162 0.0236 0.0006 0.0102 0.0112 0.0164 0.002 0.0058 0.0004 0.0259 0.0387 0.0473 0.0003
Bud 0 0.0001 0.0001 0 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0001 0 0.0001 0.0001 0.0001 0
Bud Neck 0.0003 0.0002 0.0006 0.0001 0.0016 0.0006 0.0007 0.0008 0.0001 0.0009 0.0004 0.0004 0.0004 0.0003 0.001 0.0006 0.0004 0.0004
Bud Periphery 0 0.0001 0.0001 0.0001 0.0004 0.0001 0.0002 0.0002 0.0001 0.0003 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0002 0
Bud Site 0.0002 0.0006 0.0003 0 0.0007 0.0002 0.0015 0.0011 0.0001 0.0002 0.0014 0.0001 0.0002 0.0002 0.0006 0.0001 0.0001 0
Cell Periphery 0.0011 0.001 0.0005 0.0002 0.0006 0.0003 0.0009 0.0018 0.0007 0.0007 0.0004 0.0003 0.0006 0.0012 0.0006 0.0005 0.0005 0.0009
Cytoplasm 0.0194 0.0392 0.017 0.0035 0.0111 0.02 0.0173 0.0412 0.0171 0.0533 0.0202 0.0167 0.0118 0.0841 0.0627 0.0356 0.0073 0.0226
Cytoplasmic Foci 0.007 0.0111 0.0087 0.0012 0.0084 0.0062 0.0123 0.0101 0.0065 0.0076 0.0489 0.0045 0.006 0.0051 0.0175 0.002 0.0025 0.0036
Eisosomes 0 0 0.0001 0 0.0002 0.0001 0.0001 0 0 0.0002 0.0001 0 0.0001 0 0.0001 0.0003 0.0003 0
Endoplasmic Reticulum 0.7735 0.7635 0.8268 0.9459 0.7126 0.8209 0.7189 0.7569 0.8328 0.7365 0.6386 0.8463 0.785 0.7651 0.7568 0.855 0.7453 0.82
Endosome 0.0155 0.0223 0.0206 0.0104 0.0197 0.0148 0.0462 0.0303 0.0262 0.0222 0.0777 0.0204 0.0335 0.0086 0.0148 0.0064 0.0142 0.0104
Golgi 0.0042 0.0022 0.003 0.001 0.0055 0.0036 0.0151 0.0061 0.0046 0.0284 0.028 0.0046 0.0035 0.0015 0.0048 0.0037 0.0083 0.0017
Lipid Particles 0.0812 0.0877 0.0426 0.0127 0.0633 0.0389 0.0825 0.1021 0.0716 0.0865 0.0778 0.0535 0.027 0.1025 0.0826 0.0412 0.0429 0.098
Mitochondria 0.003 0.0003 0.0008 0.0008 0.0006 0.0004 0.0017 0.0012 0.0008 0.0189 0.0012 0.0017 0.0012 0.0001 0.0009 0.0005 0.0012 0.0002
None 0.0003 0.0005 0.0016 0.0002 0.0032 0.0003 0.0046 0.0003 0.0003 0.0002 0.0034 0.0002 0.0022 0.0003 0.002 0.0002 0.002 0.0001
Nuclear Periphery 0.0731 0.0525 0.0415 0.0168 0.0795 0.0635 0.0578 0.0303 0.0195 0.0175 0.066 0.0404 0.0992 0.0212 0.0148 0.0103 0.1097 0.0339
Nucleolus 0.0001 0.0001 0.0003 0 0.0012 0.0001 0.0002 0.0004 0 0 0.0001 0 0.0004 0.0001 0.0002 0 0.0003 0
Nucleus 0.0028 0.0037 0.0041 0.0008 0.0051 0.004 0.0024 0.0034 0.0011 0.0103 0.004 0.0015 0.0089 0.0029 0.0017 0.0006 0.0023 0.0022
Peroxisomes 0.0066 0.0002 0.0004 0 0.0023 0.0022 0.0025 0.0002 0.0003 0.0007 0.0016 0.0006 0.0005 0.0001 0.0021 0.0011 0.0021 0
Punctate Nuclear 0.0011 0.0024 0.0133 0.0006 0.0417 0.0026 0.0026 0.0023 0.0005 0.0003 0.008 0.0013 0.0051 0.0009 0.007 0.0005 0.0072 0.0011
Vacuole 0.0069 0.0081 0.004 0.0038 0.0043 0.0038 0.0066 0.0099 0.0062 0.0034 0.0038 0.0043 0.0066 0.0052 0.0031 0.0019 0.0028 0.0035
Vacuole Periphery 0.0018 0.001 0.0015 0.0014 0.0013 0.0012 0.0019 0.0007 0.0011 0.0006 0.0016 0.0009 0.0018 0.0004 0.0005 0.0003 0.0027 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 75.992 100.5397 62.5067 74.007 88.6743 54.684 48.0804 75.1009 62.9169 74.1765
Translational Efficiency 2.122 1.8138 2.752 1.8692 2.1732 2.5051 3.2758 2.0409 2.3779 2.2498

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytochrome b5; involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
Localization
Cell Percentages ER (100%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Cyb5

Cyb5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cyb5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available