Standard name
Human Ortholog
Description Ubiquitin-protein ligase (E3); controls septin dynamics and spindle position checkpoint (SPOC) with ligase Dma1p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8, similar to human Chfr; contains FHA and RING finger domains; DMA2 has a paralog, DMA1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.09 0.09 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.97 0.97 0.95 0.92 0.81 0.71 0.75 0.69 0.62 0.58 0.99 0.99 1.0 0.97 0.96 0.97 0.93 0.9 0.81 0.91 0.87 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.1 0.1 0.32 0.36 0.45 0.48 0.62 0.65 0.67 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 4 4 2 2 7 5
Bud 3 3 1 0 0 5 3 12 5 7 5 3 0 0 0 1 0 0 0 2 1 0 9 9
Bud Neck 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 2 3 4 4
Bud Site 0 0 0 0 0 0 0 0 3 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 9 2 3 5 3 4 4 4 9 2 7 1 9 5 37 23 29 1 1 2 0 1 2
Cytoplasm 411 465 189 127 170 171 185 109 155 132 128 116 267 479 440 599 243 326 383 442 163 148 328 327
Endoplasmic Reticulum 0 7 1 1 1 1 0 0 1 1 0 1 2 1 0 31 15 13 1 1 4 0 3 4
Endosome 0 0 1 0 0 1 3 1 0 0 0 0 0 0 0 2 2 1 2 8 4 1 1 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4 1 6 0 0 2 0
Mitochondria 27 9 4 13 18 60 81 69 99 119 133 134 0 1 0 6 3 7 5 10 11 0 5 9
Nucleus 2 0 0 0 1 0 1 2 0 0 0 0 0 0 0 6 0 1 1 0 0 0 2 1
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 0 2 0 0 1 0 0 0 3 0 0 0 2 1 0 1 3
Vac/Vac Membrane 1 2 0 0 0 0 3 0 3 2 0 4 0 1 0 3 4 2 4 3 3 2 4 18
Unique Cell Count 426 478 194 131 179 185 228 154 207 192 205 200 269 482 440 617 252 336 414 492 202 163 378 402
Labelled Cell Count 448 498 201 144 195 241 280 197 272 272 270 269 270 491 445 692 291 384 414 492 202 163 378 402


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.9 4.8 4.7 4.7 4.3 4.1 4.1 4.0 3.5 3.6 3.5 5.8 5.2 5.3 6.9 8.1 8.0 4.7 5.0 5.6
Std Deviation (1e-4) 0.6 0.6 0.9 0.9 1.1 1.4 1.7 1.9 1.9 0.9 1.0 1.3 1.6 1.4 1.4 1.3 1.4 1.5 1.1 1.5 1.5
Intensity Change (Log2) – – – -0.03 -0.01 -0.16 -0.22 -0.22 -0.27 -0.46 -0.43 -0.45 0.28 0.13 0.15 0.53 0.76 0.75 -0.01 0.05 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.8 3.7 3.6
Cytoplasm -0.3 -1.2 -2.2 -5.3 -7.0 -6.5 -7.5 -8.6 -9.4 1.6 2.1 3.4 -0.2 -0.6 -0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.8 3.1 2.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7672 2.3587 2.3093 2.1312 2.0053 2.3984 3.9912 3.8326 4.0932 4.5256 3.7059 3.7726 4.6734 4.4024 4.2276 4.0846 4.6986 4.1553
Actin 0.0103 0.0056 0.0039 0.0002 0.0082 0.0066 0.064 0.0005 0.0195 0.0039 0.0086 0.0051 0.0506 0.0167 0.0157 0.0002 0.028 0.0173
Bud 0.0004 0.0012 0.0007 0.0023 0.0053 0.0004 0.001 0.0004 0.0026 0.0013 0.0004 0.0004 0.0034 0.0023 0.0041 0.0001 0.0004 0.0014
Bud Neck 0.0003 0.0006 0.0021 0.0004 0.0005 0.0015 0.0082 0.0001 0.0008 0.0018 0.0009 0.0022 0.0074 0.0002 0.0046 0.0001 0.0008 0.0014
Bud Periphery 0.0007 0.0013 0.0008 0.0019 0.0276 0.0006 0.0018 0.0006 0.0035 0.0022 0.0008 0.0008 0.0119 0.0049 0.004 0.0001 0.0008 0.0024
Bud Site 0.0014 0.0161 0.0135 0.0035 0.0007 0.0013 0.009 0.0015 0.0174 0.0214 0.0007 0.0002 0.0094 0.0045 0.0141 0 0.0007 0.0004
Cell Periphery 0.0005 0.0023 0.0009 0.0002 0.0003 0.0003 0.0007 0.0003 0.0005 0.0013 0.0002 0.0001 0.0018 0.0005 0.0004 0.0001 0.0001 0.0002
Cytoplasm 0.3147 0.408 0.402 0.5204 0.4261 0.535 0.3679 0.4559 0.4286 0.4282 0.4574 0.5448 0.2089 0.4682 0.3516 0.4625 0.4265 0.4862
Cytoplasmic Foci 0.0074 0.0105 0.0108 0.0711 0.0315 0.012 0.0357 0.0053 0.021 0.0072 0.0075 0.0203 0.02 0.0036 0.0131 0.0026 0.0094 0.0118
Eisosomes 0.0004 0.0004 0.0002 0.0001 0.0002 0.0002 0.0006 0.0001 0.0004 0.0002 0.0001 0.0001 0.0025 0.0002 0.0004 0.0001 0.0002 0.0002
Endoplasmic Reticulum 0.0032 0.0042 0.0043 0.0037 0.0028 0.0027 0.0097 0.0021 0.0038 0.0017 0.0025 0.0018 0.0146 0.0017 0.0026 0.0013 0.0015 0.0018
Endosome 0.0103 0.002 0.0081 0.0069 0.017 0.0054 0.0239 0.0018 0.0187 0.0075 0.0534 0.0024 0.0398 0.0041 0.0205 0.001 0.0091 0.0028
Golgi 0.0047 0.0006 0.003 0.0003 0.002 0.0011 0.0089 0.0003 0.0039 0.0035 0.0122 0.0004 0.0159 0.0114 0.0073 0.0001 0.0044 0.0015
Lipid Particles 0.0076 0.0005 0.0031 0.0007 0.001 0.0009 0.0107 0.0002 0.004 0.0031 0.0105 0.0004 0.0219 0.0001 0.0067 0.0001 0.0062 0.0016
Mitochondria 0.0047 0.0004 0.0049 0.0003 0.0065 0.0014 0.0052 0.0003 0.005 0.0064 0.008 0.0002 0.0071 0.0014 0.0076 0.0001 0.0017 0.0005
None 0.5994 0.5412 0.5159 0.3766 0.4529 0.4253 0.405 0.5275 0.4472 0.501 0.4117 0.4155 0.5225 0.4765 0.5223 0.5304 0.4941 0.4664
Nuclear Periphery 0.0015 0.0005 0.001 0.0008 0.0008 0.0008 0.0114 0.0007 0.0027 0.0004 0.0077 0.0006 0.0247 0.0005 0.0012 0.0003 0.0016 0.0005
Nucleolus 0.0017 0.0002 0.0002 0.0005 0.0002 0.0001 0.0013 0.0001 0.0007 0.0006 0.0005 0.0001 0.0013 0.0002 0.0004 0.0001 0.0002 0.0001
Nucleus 0.0021 0.0013 0.0012 0.0014 0.0009 0.0008 0.0078 0.0007 0.0012 0.001 0.0016 0.0005 0.0056 0.0013 0.0013 0.0005 0.0007 0.0005
Peroxisomes 0.0239 0.0004 0.0205 0.0005 0.0121 0.0011 0.0117 0.0001 0.0133 0.001 0.0011 0.0021 0.0127 0.0001 0.0151 0 0.0081 0.0018
Punctate Nuclear 0.0022 0.0011 0.0007 0.0055 0.0006 0.0008 0.0115 0.0004 0.0027 0.0004 0.0041 0.0012 0.012 0.0008 0.0018 0.0002 0.0041 0.0009
Vacuole 0.002 0.0016 0.0018 0.0024 0.0023 0.0014 0.0027 0.0009 0.0019 0.0052 0.0055 0.0007 0.004 0.0006 0.0038 0.0003 0.0012 0.0004
Vacuole Periphery 0.0006 0.0001 0.0003 0.0003 0.0006 0.0003 0.0012 0.0001 0.0007 0.0007 0.0045 0.0001 0.002 0.0001 0.0014 0 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.3395 14.4593 15.8609 13.8051 13.2211 16.1659 19.3871 18.4477 15.676 19.442
Translational Efficiency 1.2003 1.649 1.0505 0.8461 1.6913 1.4214 1.1333 1.044 1.2126 1.1613

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ubiquitin-protein ligase (E3); controls septin dynamics and spindle position checkpoint (SPOC) with ligase Dma1p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8, similar to human Chfr; contains FHA and RING finger domains; DMA2 has a paralog, DMA1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (78%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dma2

Dma2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dma2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available