Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; homologous to bacterial L35 and human MRPL35 ribosomal proteins

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.06 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0.05 0 0.63 0.62 0.61 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0.06 0 0 0 0 0 0 0 0 0 0.07 0.06 0.05 0.09 0.16 0.12 0.08 0.07 0.07 0.06 0.08 0.06
Golgi 0 0.08 0 0 0 0 0 0 0 0 0 0.11 0.2 0.22 0 0 0 0 0.06 0 0.08 0.08 0.06
Mitochondria 0.82 0.82 0.93 0.97 0.89 0.94 0.93 0.97 0.97 0.97 0.97 0.82 0.75 0.66 0.06 0.06 0.07 0.69 0.74 0.74 0.67 0.64 0.66
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.05 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.32 0.24 0.08 0 0.08 0.07 0 0.05 0.06 0 0.05 0.22 0.17 0.19 0.34 0.32 0.24 0.1 0.05 0 0 0.06 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 4 0 0 0 0 0 0 0 0 1 4 4 0 0 0 14 4 5 4 5 10
Bud 11 4 18 15 6 8 12 15 17 14 22 5 4 3 0 1 0 3 1 0 2 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 0 1 1 2
Bud Site 1 0 2 1 1 2 7 5 5 5 6 0 0 0 0 0 0
Cell Periphery 8 10 6 4 5 2 2 1 4 2 4 8 5 13 4 1 6 0 0 0 0 0 0
Cytoplasm 19 5 15 7 19 11 14 8 15 12 15 8 15 13 77 54 83 0 0 2 0 0 0
Endoplasmic Reticulum 18 7 2 1 5 3 3 2 3 2 7 6 5 12 7 3 5 4 1 4 1 2 1
Endosome 23 20 0 1 13 3 2 0 0 0 0 31 17 19 11 14 16 63 27 19 11 24 17
Golgi 15 28 0 1 2 0 0 0 0 0 0 49 62 79 0 0 1 28 22 6 14 26 17
Mitochondria 564 272 267 289 336 331 437 363 500 362 471 352 229 238 7 5 10 518 279 214 121 197 197
Nucleus 6 2 2 1 3 2 6 7 13 6 6 1 1 2 1 1 1 2 0 0 0 0 1
Nuclear Periphery 7 11 2 6 6 17 20 27 25 16 16 7 5 7 0 0 0 15 2 5 4 1 1
Nucleolus 8 6 1 15 13 15 20 15 13 14 9 9 7 6 0 1 1 2 1 1 1 1 3
Peroxisomes 4 9 0 4 5 4 4 0 3 1 2 8 2 11 5 3 6 8 8 12 8 19 14
SpindlePole 5 4 5 2 4 5 13 10 15 5 8 5 1 2 1 1 5 7 1 3 1 3 2
Vac/Vac Membrane 219 79 24 10 31 25 18 18 30 10 24 96 52 67 42 28 33 75 19 7 6 17 19
Unique Cell Count 685 333 288 297 378 351 469 373 517 375 487 427 305 362 122 87 137 754 378 289 182 308 299
Labelled Cell Count 910 458 348 357 449 428 558 471 643 449 590 586 409 476 155 112 168 754 378 289 182 308 299


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 6.5 4.2 5.0 5.4 4.3 4.1 3.4 3.6 3.6 3.5 6.7 6.9 6.5 6.4 6.7 6.6 6.6 6.8 6.5
Std Deviation (1e-4) 0.9 1.0 0.9 1.3 1.3 1.1 1.0 0.9 0.9 0.9 0.8 1.1 1.2 1.0 1.5 1.7 2.1 1.3 1.2 1.2
Intensity Change (Log2) 0.23 0.36 0.04 -0.05 -0.3 -0.22 -0.25 -0.3 0.65 0.71 0.61 0.6 0.66 0.65 0.63 0.69 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -0.6 -3.2 0 0 0 0 0 0 -3.8 -3.2 -3.9 -2.8 0 -3.0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 -0.2 -0.4 1.1 0 0 0
Cytoplasm -1.8 -0.1 -1.3 -1.5 -2.1 -1.7 -1.3 -1.5 -2.5 -0.2 -1.0 12.8 12.0 12.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 2.3 0 0 0
Endosome 0 3.2 0 0 0 0 0 0 4.7 4.1 3.9 0 0 5.9
Golgi 0 0 0 0 0 0 0 0 6.0 8.1 8.5 0 0 0
Mitochondria 2.6 -1.7 0 0 0 0 0 0 -4.0 -5.8 -8.2 -17.1 -15.9 -17.3
Nucleus 0 0 0 0 0 1.8 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.5 2.8 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.6 -0.1 -0.6 -2.6 -1.8 -1.4 -3.3 -1.9 5.0 3.2 3.7 6.6 5.6 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0558 2.0672 2.0825 1.6344 1.622 2.2007 1.7903 1.2299 1.4381 1.413 1.5499 1.3733 2.6686 2.8976 2.6306 2.6432 2.2044 3.0026
Actin 0.0041 0.0036 0.0014 0.002 0.0019 0.0013 0.0225 0.0085 0.0256 0.006 0.0132 0.0062 0.0106 0.0133 0.0119 0.0005 0.0027 0.0053
Bud 0.0003 0.0008 0.0013 0.0004 0.0011 0.0002 0.0009 0.0015 0.0015 0.0026 0.004 0.0007 0.0013 0.0031 0.0007 0.0035 0.0004 0.0002
Bud Neck 0.0006 0.0004 0.0013 0.0006 0.0005 0.0003 0.0026 0.0004 0.0045 0.0003 0.0031 0.0008 0.0014 0.0009 0.0006 0.0001 0.0002 0.0013
Bud Periphery 0.0007 0.0017 0.0025 0.0007 0.0021 0.0005 0.0016 0.0031 0.0044 0.0186 0.0044 0.0013 0.0046 0.0074 0.0018 0.0177 0.0009 0.0006
Bud Site 0.0018 0.0126 0.0016 0.0006 0.0022 0.0002 0.0031 0.0128 0.0051 0.0024 0.0041 0.0009 0.0068 0.0198 0.0174 0.0019 0.0008 0.0003
Cell Periphery 0.0005 0.0017 0.0003 0.0002 0.0002 0.0002 0.0005 0.0007 0.0027 0.0006 0.0014 0.0002 0.0032 0.0025 0.0008 0.0006 0.0001 0.0002
Cytoplasm 0.0033 0.0039 0.0011 0.0006 0.0195 0.0029 0.0048 0.0099 0.0093 0.0061 0.0207 0.0037 0.0158 0.0168 0.0086 0.0023 0.0002 0.0053
Cytoplasmic Foci 0.025 0.0363 0.0158 0.0183 0.0252 0.0125 0.0519 0.0298 0.0302 0.0262 0.0372 0.0221 0.0287 0.0558 0.0223 0.0037 0.0357 0.0164
Eisosomes 0.0003 0.0005 0.0001 0.0001 0.0001 0.0001 0.0004 0.0004 0.0006 0.0005 0.0002 0.0002 0.001 0.0017 0.0006 0.0001 0.0001 0.0003
Endoplasmic Reticulum 0.0011 0.0044 0.0007 0.0004 0.0028 0.0009 0.0058 0.0042 0.0049 0.0113 0.0006 0.0023 0.0039 0.0059 0.0057 0.0005 0 0.0016
Endosome 0.0684 0.0748 0.0396 0.0362 0.0946 0.0504 0.1411 0.0876 0.0922 0.1267 0.0314 0.0662 0.0911 0.0907 0.053 0.0171 0.0193 0.0504
Golgi 0.0368 0.0572 0.0343 0.0394 0.0432 0.04 0.0774 0.0706 0.0402 0.0471 0.0181 0.0515 0.0591 0.0628 0.0332 0.0162 0.0353 0.0351
Lipid Particles 0.0456 0.0153 0.025 0.0082 0.0057 0.0209 0.0533 0.0141 0.0234 0.0455 0.0402 0.0157 0.0333 0.0298 0.0158 0.001 0.0106 0.022
Mitochondria 0.7506 0.7402 0.8342 0.8381 0.7552 0.8333 0.5432 0.704 0.6938 0.6403 0.738 0.7919 0.661 0.5701 0.7021 0.9092 0.6899 0.796
None 0.0105 0.0018 0.0008 0.0001 0.005 0.0003 0.0048 0.003 0.0071 0.0189 0.0028 0.0038 0.0255 0.0505 0.0618 0.0018 0.0002 0.0269
Nuclear Periphery 0.0025 0.003 0.0005 0.0001 0.0012 0.0022 0.0241 0.0038 0.0069 0.0081 0.0012 0.0022 0.0027 0.004 0.0033 0.0003 0 0.0007
Nucleolus 0.0006 0.0008 0.0006 0.0001 0.0003 0.0002 0.0011 0.0007 0.0011 0.0008 0.0228 0.0002 0.0014 0.0013 0.0017 0.0006 0.0001 0.0006
Nucleus 0.0018 0.0005 0.0003 0.0002 0.0008 0.0005 0.0059 0.0019 0.0014 0.0013 0.003 0.0005 0.0015 0.0015 0.0026 0.0014 0.0001 0.0004
Peroxisomes 0.0282 0.029 0.0249 0.0486 0.0193 0.0225 0.029 0.0222 0.0244 0.0143 0.0279 0.0195 0.0211 0.0443 0.0266 0.0121 0.2006 0.0251
Punctate Nuclear 0.0018 0.0003 0.0003 0.0001 0.0002 0.0001 0.0045 0.0004 0.0005 0.0013 0.0043 0.0003 0.0005 0.0007 0.0012 0.0001 0.0001 0.0021
Vacuole 0.0086 0.0063 0.0073 0.0026 0.0127 0.0038 0.012 0.0119 0.0118 0.013 0.0163 0.0046 0.0166 0.0097 0.0182 0.0067 0.0009 0.003
Vacuole Periphery 0.0068 0.0049 0.0062 0.0024 0.0062 0.0067 0.0095 0.0086 0.0084 0.008 0.0052 0.0051 0.0089 0.0074 0.01 0.0028 0.002 0.0062

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.7803 8.5032 8.413 18.0886 16.4438 3.1689 19.3446 29.0141 17.1645 23.7684
Translational Efficiency 2.1987 4.396 4.617 2.4971 2.9977 19.2267 2.4918 2.2041 2.9714 2.2681

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
347 598 2523 83 1729 1751 2460 1706 2076 2349 4983 1789

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 662.86 815.07 985.51 1283.83 698.02 794.28 1098.30 1206.02 692.14 799.57 1041.19 1209.63
Standard Deviation 82.69 117.68 139.82 161.87 101.96 110.19 150.82 194.07 99.86 112.51 155.91 193.39
Intensity Change Log 2 0.298220 0.572166 0.953678 0.186379 0.653932 0.788914 0.241938 0.614684 0.871519

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000523 0.001087 0.001157 0.023498 0.000596 0.001039 0.001505 0.004508 0.000584 0.001052 0.001329 0.005389
Bud Neck 0.019151 0.006036 0.004473 0.007733 0.006586 0.004230 0.004117 0.003064 0.008687 0.004690 0.004297 0.003280
Bud Site 0.006934 0.013089 0.015330 0.033308 0.008032 0.009216 0.010608 0.012387 0.007849 0.010202 0.012999 0.013358
Cell Periphery 0.000543 0.000398 0.000181 0.000347 0.000504 0.000373 0.000146 0.000209 0.000511 0.000379 0.000164 0.000215
Cytoplasm 0.011058 0.005340 0.004717 0.002548 0.003566 0.002697 0.001444 0.001363 0.004818 0.003370 0.003101 0.001418
Cytoplasmic Foci 0.040716 0.040544 0.047269 0.075187 0.031562 0.031301 0.022797 0.018287 0.033092 0.033654 0.035188 0.020927
Eisosomes 0.000112 0.000073 0.000049 0.000140 0.000089 0.000067 0.000042 0.000060 0.000093 0.000069 0.000045 0.000064
Endoplasmic Reticulum 0.000491 0.001189 0.001713 0.002180 0.000745 0.000602 0.000508 0.000898 0.000702 0.000751 0.001118 0.000957
Endosome 0.077484 0.100838 0.072320 0.049815 0.071081 0.081887 0.039506 0.023545 0.072152 0.086712 0.056120 0.024764
Golgi 0.067154 0.075836 0.092312 0.084846 0.068865 0.078149 0.032225 0.049965 0.068579 0.077560 0.062648 0.051583
Lipid Particles 0.008527 0.007387 0.001353 0.012226 0.005291 0.003498 0.001568 0.001320 0.005832 0.004488 0.001459 0.001826
Mitochondria 0.659027* 0.639683* 0.514933* 0.468295* 0.734773* 0.710412* 0.756862* 0.795545* 0.722112* 0.692406* 0.634368* 0.780363*
Mitotic Spindle 0.008436 0.020905 0.086889 0.099828 0.005580 0.009835 0.050311 0.032266 0.006058 0.012653 0.068831 0.035400
None 0.000529 0.000524 0.000532 0.000471 0.001172 0.000338 0.000372 0.000476 0.001065 0.000385 0.000453 0.000476
Nuclear Periphery 0.000332 0.000976 0.001660 0.001241 0.000161 0.000109 0.001337 0.001663 0.000190 0.000330 0.001501 0.001643
Nuclear Periphery Foci 0.000409 0.000977 0.001651 0.001541 0.000418 0.000241 0.001146 0.001182 0.000416 0.000428 0.001402 0.001199
Nucleolus 0.002306 0.000640 0.001087 0.001608 0.002458 0.000604 0.000730 0.000501 0.002433 0.000613 0.000911 0.000553
Nucleus 0.007587 0.004963 0.003622 0.001432 0.002128 0.000669 0.001046 0.000943 0.003041 0.001762 0.002350 0.000965
Peroxisomes 0.068550 0.048508 0.010816 0.060904 0.027801 0.038590 0.012477 0.010612 0.034613 0.041115 0.011636 0.012946
Vacuole 0.009279 0.017620 0.076538 0.038843 0.013373 0.013583 0.032443 0.022732 0.012689 0.014611 0.054769 0.023479
Vacuole Periphery 0.010851 0.013387 0.061399 0.034010 0.015216 0.012561 0.028811 0.018474 0.014486 0.012771 0.045311 0.019195

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.11 -3.31 -3.65 -3.56 -3.55 -1.26 -3.70 -5.06 -4.22 -3.82 -1.69 -4.34 -6.04 -5.28 -5.10
Bud Neck 2.78 3.15 1.98 -1.31 -2.11 2.13 2.48 3.41 1.23 1.63 3.30 3.87 4.51 1.67 1.75
Bud Site -2.32 -5.26 -2.55 -1.95 -1.76 -0.82 -2.11 -2.96 -2.18 -1.20 -1.83 -5.09 -3.74 -2.22 -0.50
Cell Periphery 1.11 3.89 1.88 0.46 -2.36 1.89 6.25 5.01 3.85 -3.80 2.09 6.62 5.71 3.95 -2.29
Cytoplasm 1.35 1.60 1.93 1.31 1.43 1.14 3.06 3.40 2.44 0.52 1.47 1.83 3.96 3.59 5.27
Cytoplasmic Foci -0.21 -1.48 -2.77 -2.74 -2.34 0.17 4.04 6.08 6.60 2.45 -0.36 -0.98 5.84 6.78 8.23
Eisosomes 2.09 3.47 -0.98 -3.05 -4.24 1.50 3.27 1.94 1.42 -4.05 1.93 3.82 2.24 1.04 -4.22
Endoplasmic Reticulum -2.65 -7.47 -3.23 -1.96 -1.23 0.52 1.59 -1.13 -1.18 -2.85 -0.28 -3.68 -2.01 -1.06 1.09
Endosome -2.31 0.66 2.86 5.44 3.31 -2.30 8.64 13.40 15.73 7.21 -3.40 4.88 14.40 18.31 15.74
Golgi -0.97 -3.48 -0.86 -0.37 0.66 -2.12 10.55 4.30 6.23 -4.81 -2.28 1.86 4.12 6.20 3.06
Lipid Particles 0.30 3.56 -0.65 -0.81 -2.13 1.83 4.30 4.78 3.90 0.72 1.28 5.62 4.97 3.99 -1.02
Mitochondria 0.90 7.11 4.39 4.06 1.44 2.25 -2.12 -5.44 -7.54 -3.77 3.06 10.31 -5.25 -8.17 -15.58
Mitotic Spindle -4.04 -20.53* -4.58 -3.98 -0.95 -3.40 -16.59 -10.32 -8.19 4.75 -5.47 -27.81 -11.07 -8.19 9.41
None 0.04 0.03 0.55 0.40 0.82 2.13 2.06 1.80 -3.11 -4.84 2.07 1.89 1.83 -1.88 -0.84
Nuclear Periphery -1.07 -5.01 -2.26 -0.43 0.73 1.57 -4.35 -3.28 -3.39 -0.65 -0.93 -7.48 -3.33 -2.83 -0.34
Nuclear Periphery Foci -1.81 -5.53 -2.07 -1.04 -0.20 1.38 -2.94 -2.85 -3.70 -0.19 -0.28 -6.11 -3.11 -3.02 0.60
Nucleolus 1.86 1.45 0.20 -1.34 -0.98 2.65 2.56 2.67 0.23 0.62 3.02 2.62 2.91 0.10 1.10
Nucleus 0.59 1.21 1.00 0.51 -0.03 2.84 1.84 1.69 -0.81 0.14 1.39 0.79 2.41 1.02 2.46
Peroxisomes 2.09 7.25 0.63 -0.70 -3.59 -3.21 6.49 7.10 9.52 1.20 -2.05 9.84 8.44 10.33 -0.78
Vacuole -3.31 -24.39 -2.81 -2.24 1.65 -0.14 -10.56 -4.93 -4.83 3.89 -1.48 -24.89 -5.93 -4.95 12.83
Vacuole Periphery -1.22 -19.25 -1.92 -1.71 2.48 2.06 -7.89 -2.10 -3.93 5.44 1.56 -19.78 -3.03 -4.31 14.15
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; homologous to bacterial L35 and human MRPL35 ribosomal proteins
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrp35

Mrp35


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrp35-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available