ORF
Human Ortholog
Description NADH-dependent aldehyde reductase, involved in detoxification of furfural; expression is up-regulated in the presence of furfural and 5-hydroxymethylfurfural, which are compounds generated during lignocellulosic biomass pre-treatment; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.07 0.07 0 0 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.96 0.92 0.82 0.84 0.78 0.75 0.71 0.67 0.79 0.66 0.97 0.94 0.92 0.94 0.99 0.99 0.8 0.86 0.85 0.94 0.91 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.06 0.08 0.07 0.07 0.12 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.08 0.17 0.14 0.14 0.16 0.17 0.22 0.1 0.18 0 0.06 0.06 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 1 3 4 6 5 5 0 0 1 0 0 0 4 2 2 0 0 2
Bud 5 0 0 1 0 2 3 3 1 4 3 3 0 1 0 0 0 0 37 18 17 2 10 15
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 1
Bud Site 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 2 7 5 10 6 11 6 5 7 1 2 2 6 6 3 0 0 0 0 0 0
Cytoplasm 339 249 238 251 308 258 322 290 259 283 231 201 209 265 314 142 270 136 289 220 213 102 255 204
Endoplasmic Reticulum 2 0 2 0 4 2 16 23 29 28 19 38 0 1 3 8 10 5 0 1 1 0 0 0
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 4 1 1 0 0 4 2 1 0 0 1
Golgi 0 0 0 0 0 0 1 1 5 5 2 6 0 0 0 0 0 0 3 1 0 0 0 0
Mitochondria 11 1 0 4 5 20 12 9 12 9 7 5 0 0 0 2 0 0 4 1 2 1 1 3
Nucleus 1 0 0 0 2 2 6 5 3 7 2 3 0 2 2 1 0 0 1 1 1 0 1 1
Nuclear Periphery 1 0 0 0 1 0 1 2 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 1 0 0 0 0 0 1 1 4 0 0 0 3 1 3 0 2 3
Vac/Vac Membrane 4 2 11 22 63 43 59 63 61 93 29 54 5 16 19 7 3 0 0 2 3 0 1 0
Unique Cell Count 352 252 248 273 374 308 412 386 365 422 291 306 215 282 342 151 273 137 361 255 251 109 280 241
Labelled Cell Count 364 254 252 283 390 332 432 405 385 441 303 324 216 293 347 168 289 144 361 255 251 109 280 241


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.2 11.0 9.6 9.9 12.9 17.1 22.6 25.6 27.3 29.6 31.3 35.5 7.4 12.4 14.2 78.4 84.4 76.1 7.3 7.6 7.8
Std Deviation (1e-4) 7.7 7.6 5.2 5.6 7.9 9.5 10.7 11.4 12.8 12.2 13.0 14.6 4.6 11.4 12.1 36.5 31.7 28.7 3.5 3.7 3.6
Intensity Change (Log2) – – – 0.03 0.42 0.82 1.23 1.41 1.5 1.62 1.7 1.88 -0.37 0.36 0.56 3.02 3.13 2.98 -0.39 -0.35 -0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.9 -5.1 -4.6 -6.2 -6.9 -7.8 -8.7 -5.7 -8.7 0.7 -1.0 -2.0 -0.9 2.1 1.9
Endoplasmic Reticulum 0 0 0 2.4 3.3 4.0 3.5 3.4 5.3 0 0 0 0 2.2 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.7 4.7 3.8 4.0 4.5 4.6 6.1 2.4 4.8 -1.2 0.6 0.6 0.1 -2.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 70.6347 94.9237 85.215 86.8209 79.5682 85.6592 85.589 100.2662 92.2797 84.9176 89.7311 95.088 49.5954 65.3527 61.3995 51.8631 42.8798 58.722
Actin 0.0028 0.0002 0.0004 0.0002 0.0001 0.0002 0.0002 0.0006 0.0002 0.0002 0.0012 0.0001 0.0093 0.0007 0.0018 0.041 0.019 0.0013
Bud 0.0008 0.0007 0.0006 0.0038 0 0.0004 0.0001 0.0013 0.0008 0.0002 0.0013 0.0001 0.0015 0.0054 0.0077 0.0012 0.0157 0.0014
Bud Neck 0.0003 0.0001 0.0001 0.0002 0.0001 0.0005 0.0002 0.0002 0.0003 0.0003 0.0002 0.0006 0.0006 0.0002 0.0003 0.001 0.0005 0.0009
Bud Periphery 0.0015 0.0004 0.0001 0.0012 0 0.0001 0 0.0004 0.0005 0 0.0005 0.0001 0.0009 0.0014 0.0043 0.0008 0.007 0.0005
Bud Site 0.0009 0.0001 0.0002 0.0001 0 0 0 0.0013 0.0001 0 0.0007 0 0.0016 0.0039 0.0025 0.0022 0.001 0.0002
Cell Periphery 0.0016 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0003 0.0002 0.0001 0.0004 0.0001 0.0005 0.0002 0.0002 0.0003 0.0007 0.0001
Cytoplasm 0.9113 0.9935 0.988 0.9827 0.9894 0.973 0.9698 0.9842 0.9818 0.978 0.9764 0.9661 0.8785 0.9772 0.9658 0.8546 0.8485 0.955
Cytoplasmic Foci 0.0084 0.0002 0.0017 0.0001 0.0003 0.0008 0.0007 0.0003 0.0003 0.0002 0.001 0.0001 0.0138 0.001 0.0016 0.0133 0.0104 0.0015
Eisosomes 0.0002 0 0 0 0 0 0 0 0 0 0 0 0.0004 0 0 0.0004 0.0007 0
Endoplasmic Reticulum 0.0021 0.0002 0.0001 0.0001 0.0001 0.0003 0.001 0.0006 0.0004 0.0013 0.0007 0.0008 0.0044 0.0003 0.0005 0.0034 0.0024 0.0022
Endosome 0.0037 0.0001 0.0001 0 0 0.0003 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0091 0.0002 0.0001 0.0053 0.0068 0.0037
Golgi 0.002 0 0 0 0 0 0 0 0 0 0 0 0.0037 0.0002 0 0.0052 0.0048 0.0006
Lipid Particles 0.0056 0 0.0001 0 0 0.0001 0.0001 0 0 0 0.0001 0 0.0053 0.0011 0 0.0182 0.0061 0.0009
Mitochondria 0.0055 0 0.0002 0.0001 0 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0062 0.0002 0.0002 0.0062 0.008 0.0006
None 0.0038 0.0002 0.0005 0.0002 0.0002 0.0007 0.0011 0.0004 0.0002 0.0004 0.0016 0.0002 0.0095 0.0004 0.002 0.0059 0.0131 0.001
Nuclear Periphery 0.0048 0.0009 0.001 0.0017 0.0014 0.0024 0.0043 0.0018 0.002 0.0034 0.003 0.0046 0.009 0.0009 0.0019 0.0047 0.0106 0.0061
Nucleolus 0.0076 0 0.0012 0 0 0.0021 0.0001 0 0.0001 0.0001 0.0003 0 0.0034 0.0001 0.0001 0.0006 0.0166 0.0006
Nucleus 0.0187 0.0029 0.0043 0.0087 0.0076 0.0154 0.0206 0.0071 0.0109 0.014 0.0094 0.0257 0.0282 0.0043 0.0082 0.0117 0.0127 0.0176
Peroxisomes 0.0023 0 0 0 0 0 0 0 0 0 0 0 0.0023 0.0009 0.0001 0.0183 0.0007 0.0002
Punctate Nuclear 0.003 0.0001 0.0002 0.0001 0.0002 0.0006 0.0003 0.0002 0.0001 0.0002 0.0009 0.0002 0.0063 0.0004 0.0015 0.0022 0.0028 0.0031
Vacuole 0.0091 0.0003 0.0008 0.0003 0.0003 0.0019 0.001 0.0009 0.0015 0.0013 0.0018 0.0011 0.004 0.0006 0.0011 0.0024 0.0064 0.0019
Vacuole Periphery 0.0039 0 0.0002 0.0001 0 0.0007 0.0001 0 0.0003 0.0001 0.0002 0.0001 0.0014 0.0001 0.0001 0.0012 0.0054 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.5606 31.3966 51.1253 70.7767 65.1428 41.9276 31.9655 40.1733 58.4058 42.7909
Translational Efficiency 1.994 1.692 1.5566 1.569 1.5034 1.7808 1.755 1.6558 1.4239 1.5263

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description NADH-dependent aldehyde reductase, involved in detoxification of furfural; expression is up-regulated in the presence of furfural and 5-hydroxymethylfurfural, which are compounds generated during lignocellulosic biomass pre-treatment; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (66%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YNL134C

YNL134C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ynl134c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available