Standard name
Human Ortholog
Description Linker protein responsible for recruitment of myosin to the bud neck; interacts with the C-terminal extensions of septins Cdc11p and Shs1p and binds Myo1p to promote cytokinesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud 0.05 0.05 0.08 0.06 0.12 0.11 0.13 0.09 0.11 0.09 0.07 0.07 0 0 0 0 0 0.08 0.24 0.2 0.2 0 0 0
Bud Neck 0.13 0.12 0.09 0.14 0.2 0.12 0.13 0.11 0.15 0.12 0.15 0.13 0.17 0.19 0.16 0.17 0.1 0.08 0.23 0.22 0.2 0.05 0 0
Bud Site 0 0 0 0.06 0 0 0 0 0.05 0 0 0 0.05 0 0.05 0 0.05 0 – – – – – –
Cell Periphery 0 0.05 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.63 0.68 0.69 0.62 0.45 0.53 0.49 0.44 0.4 0.46 0.45 0.41 0.55 0.47 0.43 0.55 0.74 0.65 0.4 0.47 0.44 0.85 0.92 0.98
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0.13 0.11 0.25 0.32 0.29 0.29 0.38 0.41 0.45 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0.08 0.05 0.07 0.06 0.08 0.07 0.05 0.06 0 0 0.08 0.07 0.05 0.08 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.1 0.07 0.1 0.09 0.06 0.1 0.12 0.08 0.05 0.09 0.1 0.2 0.19 0 0 0 0 0 0.05 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0.05 0.05 0 0 0 0.05 0 0.06 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 14 2 2 6 2 4 1 6 8 1 2 8 5 10 2 4 3 3 5 7 0 1 0
Bud 6 7 20 9 25 30 40 32 46 46 29 35 7 4 10 2 3 5 30 34 55 8 5 1
Bud Neck 15 17 23 22 42 31 41 39 63 63 60 63 37 36 37 14 10 5 30 37 54 10 3 0
Bud Site 5 5 11 9 9 8 5 7 22 21 12 15 10 2 11 3 5 1 – – – – – –
Cell Periphery 2 7 13 5 1 3 10 8 11 24 10 18 6 7 4 3 6 1 0 0 1 0 0 0
Cytoplasm 74 99 172 100 95 142 150 153 165 236 178 196 120 88 98 45 71 40 52 80 122 174 329 338
Endoplasmic Reticulum 1 0 0 0 2 2 1 1 3 2 1 1 0 0 0 3 4 2 0 2 5 2 0 1
Endosome 4 1 9 4 15 10 6 10 7 5 5 4 16 10 8 3 0 2 1 2 2 1 2 0
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 0 2 0 2 0
Mitochondria 11 4 8 21 22 68 99 100 119 196 165 214 0 1 3 3 4 2 0 0 4 0 0 0
Nucleus 1 0 1 2 4 6 8 11 13 16 9 4 7 4 11 1 0 0 0 0 0 0 1 1
Nuclear Periphery 0 0 0 1 2 0 2 2 0 0 2 4 0 1 2 0 0 0 0 0 0 1 0 0
Nucleolus 7 4 3 6 16 13 22 21 31 36 20 30 9 5 19 6 5 5 1 1 0 0 0 0
Peroxisomes 3 2 10 6 8 4 7 5 6 5 4 8 6 4 9 2 1 1 0 0 0 0 0 0
SpindlePole 2 0 24 12 21 24 18 33 48 41 20 44 23 38 43 3 2 2 5 3 13 1 3 0
Vac/Vac Membrane 2 3 4 2 4 7 12 23 19 28 17 21 4 9 9 5 3 5 0 1 1 3 0 1
Unique Cell Count 118 146 248 161 209 269 309 346 408 516 398 478 220 187 227 82 96 62 129 170 277 206 359 347
Labelled Cell Count 137 163 301 201 272 350 425 446 559 727 533 659 253 214 274 97 120 74 129 170 277 206 359 347


Ambiguous, Bud, Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 10.6 8.4 7.6 7.4 6.4 6.0 5.9 5.9 5.5 5.1 5.1 10.3 10.5 9.7 14.5 14.4 14.0 10.0 10.3 10.4
Std Deviation (1e-4) 1.3 1.7 1.2 1.8 2.5 1.7 1.9 1.6 1.9 1.2 1.3 1.2 1.8 2.0 2.1 3.2 2.8 3.5 1.4 1.6 1.4
Intensity Change (Log2) – – – -0.15 -0.17 -0.39 -0.47 -0.5 -0.5 -0.6 -0.71 -0.71 0.3 0.32 0.21 0.79 0.78 0.73 0.26 0.3 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0 0
Bud -1.0 1.4 1.2 1.8 0.5 1.3 0.4 -0.4 -0.4 -2.3 -2.7 -1.6 -1.8 -1.6 0
Bud Neck 1.4 3.3 0.8 1.5 0.8 2.3 1.2 2.1 1.5 2.4 3.0 2.3 1.9 0.3 -0.3
Bud Site 0.5 -0.1 -0.9 -2.0 -1.7 0.5 -0.2 -0.9 -0.9 0.1 -2.0 0.2 0 0 0
Cell Periphery -1.0 -2.9 -2.7 -1.2 -1.9 -1.7 -0.4 -1.8 -0.9 -1.4 -0.7 -2.0 0 0.4 0
Cytoplasm -1.5 -5.2 -3.9 -4.9 -6.1 -7.2 -6.1 -6.1 -7.2 -3.3 -4.7 -5.8 -2.4 0.8 -0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.6 1.7 0.1 -1.2 -0.5 -1.5 0 -2.0 0 1.7 0.9 -0.1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.8 3.1 7.1 8.6 8.0 8.2 10.2 10.7 11.5 0 0 -1.4 0 0 0
Nucleus 0 0 0 0 2.4 2.4 2.4 0 0 0 0 3.1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 3.4 2.4 3.3 3.0 3.6 3.4 2.5 3.1 2.0 0 3.7 0 0 0
Peroxisomes -0.2 -0.1 -1.8 -1.2 -2.0 -2.1 0 -2.6 -1.9 -0.8 -1.1 0 0 0 0
SpindlePole -0.8 0.1 -0.3 -1.7 -0.1 0.8 -0.8 -2.3 -0.2 0.3 3.1 2.9 -1.7 -2.4 -1.6
Vacuole 0 0 0.8 1.6 2.9 2.1 2.5 1.9 1.9 0 1.9 1.6 0 0 0