Standard name
Human Ortholog
Description Zn2+-dependent endopolyphosphatase; required with PPN1 to mobilize polyphosphate stores in response to phosphate starvation; member of the PPP-superfamily of metalloproteases; localizes to the vacuolar lumen via the MVB pathway; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.96 0.97 0.97 0.96 0.96 0.94 0.93 0.86 0.86 0.84 0.86 0.92 0.95 0.98 – 0.79 0.82 0.35 0.49 0.46 0.37 0.36 0.36
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0 0.08 0.1 0.15 0.16 0.22 0.2 0.27 0.29 0 0 0 – 0 0 0 0 0 0.05 0.07 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.08 0 – 0.23 0.24 0.57 0.36 0.41 0.44 0.43 0.43
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 5 1 2 3 3
Bud 0 0 1 0 2 4 6 4 5 9 8 3 0 0 0 – 0 0 1 3 0 4 3 8
Bud Neck 0 0 0 0 0 1 2 3 1 0 0 2 0 1 0 – 0 0 1 4 2 1 0 1
Bud Site 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 1 0 1 0 1 1 0 3 0 0 0 1 0 0 – 0 1 0 0 0 0 0 0
Cytoplasm 160 274 198 239 343 597 678 647 370 462 391 427 172 184 177 – 103 101 62 143 89 73 77 87
Endoplasmic Reticulum 2 2 1 0 1 0 0 0 1 0 0 0 3 1 0 – 3 5 0 0 0 4 2 7
Endosome 0 2 0 0 0 1 0 0 0 0 0 0 0 2 0 – 0 0 4 8 3 2 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 1 0 0 0 2 2 1 2
Mitochondria 7 2 10 4 30 61 110 111 94 107 126 143 2 1 0 – 5 4 5 10 7 9 14 11
Nucleus 0 2 0 0 1 3 4 2 1 7 1 3 4 1 0 – 4 5 0 0 0 0 2 0
Nuclear Periphery 0 0 0 0 1 3 3 0 3 3 1 0 0 0 0 – 0 0 0 0 0 1 1 0
Nucleolus 0 0 0 0 0 0 1 2 2 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 1 2 0 4 3 4 0 0 0 0 0 – 1 0 0 4 1 0 2 3
Vac/Vac Membrane 7 11 4 4 9 19 10 16 18 23 23 18 11 16 7 – 30 29 102 107 80 86 92 103
Unique Cell Count 168 286 204 246 357 622 723 696 428 535 464 499 187 193 180 130 123 181 295 195 197 216 244
Labelled Cell Count 177 294 215 250 388 692 815 790 501 615 550 596 193 206 184 147 145 181 295 195 197 216 244


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.4 3.2 3.2 3.2 3.4 2.8 3.0 2.9 2.8 2.8 2.8 5.4 4.4 4.7 4.7 4.9 4.9 5.7 5.7 6.1
Std Deviation (1e-4) 0.4 1.0 0.8 1.2 1.1 1.6 1.2 1.2 1.2 0.8 1.0 1.2 2.2 1.0 1.5 0.8 1.1 1.2 2.1 2.3 2.8
Intensity Change (Log2) – – – -0.0 0.0 0.07 -0.2 -0.09 -0.15 -0.21 -0.19 -0.19 0.74 0.47 0.55 0.55 0.63 0.61 0.83 0.83 0.92


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 6.2 6.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1581 -0.2374 -0.1235 -0.0379 -0.119 0.0956 1.264 0.6924 1.3266 1.0912 1.3107 1.4665 -1.0049 -1.3705 -1.9084 0.5793 -0.4319 -1.9342
Actin 0.0094 0.0008 0.005 0.0014 0.0218 0.0002 0.0049 0.0004 0.023 0.0529 0.0174 0.0004 0.0237 0.0001 0.0002 0.0211 0.1129 0
Bud 0.0034 0.0029 0.0041 0.005 0.0093 0.0018 0.0021 0.0004 0.0006 0.0017 0.0043 0.0009 0.0006 0.0001 0 0.0008 0.0024 0
Bud Neck 0.0043 0.0002 0.0007 0.0016 0.0004 0.0051 0.0018 0.0002 0.0004 0.0005 0.0004 0.0009 0.0041 0.0004 0 0.0003 0.0006 0.0002
Bud Periphery 0.0067 0.0036 0.0037 0.0027 0.0045 0.0027 0.0078 0.0006 0.0005 0.0051 0.005 0.0074 0.0014 0.0002 0.0001 0.0024 0.0033 0
Bud Site 0.0069 0.008 0.0031 0.0254 0.0053 0.0007 0.0033 0.0183 0.0027 0.0008 0.0043 0.0001 0.0183 0.0014 0.0001 0.0011 0.0063 0
Cell Periphery 0.0007 0.0002 0.0002 0.0028 0.0003 0.0002 0.001 0.0005 0.0003 0.0004 0.0007 0.0002 0.0015 0.0002 0.0001 0.0005 0.0005 0
Cytoplasm 0.0386 0.0314 0.0317 0.0453 0.0465 0.0716 0.0682 0.1769 0.1069 0.0782 0.1115 0.1164 0.047 0.0372 0.0423 0.0067 0.0191 0.1393
Cytoplasmic Foci 0.0194 0.0079 0.0083 0.024 0.0217 0.0147 0.0262 0.0098 0.0327 0.0357 0.0426 0.0188 0.0691 0.0052 0.003 0.0613 0.083 0.0385
Eisosomes 0.0003 0.0002 0.0002 0.0001 0.0006 0.0001 0.0003 0.0001 0.0002 0.0002 0.0004 0.0001 0.0044 0.0001 0.0003 0.0003 0.0005 0
Endoplasmic Reticulum 0.0016 0.0004 0.0011 0.0004 0.0015 0.0005 0.0076 0.0033 0.0016 0.0022 0.0057 0.0016 0.0047 0.0013 0.0005 0.0024 0.0076 0.0003
Endosome 0.0146 0.0014 0.0047 0.0153 0.0307 0.0037 0.037 0.0134 0.0296 0.0788 0.0426 0.0222 0.0335 0.0121 0.0006 0.0691 0.0183 0.0218
Golgi 0.0029 0.0001 0.0014 0.0016 0.0059 0.0002 0.0015 0.0004 0.0026 0.0022 0.0132 0.0005 0.0059 0.0015 0.0001 0.091 0.0414 0.0002
Lipid Particles 0.0084 0.0005 0.0025 0.0045 0.0239 0.0019 0.0053 0.0009 0.0016 0.0036 0.0059 0.001 0.0208 0.007 0.0004 0.0183 0.0189 0.0012
Mitochondria 0.0028 0.0009 0.0034 0.0013 0.0215 0.0005 0.0051 0.0006 0.0018 0.0048 0.031 0.0029 0.0019 0.0043 0.0001 0.0894 0.0173 0.0001
None 0.8444 0.9327 0.9114 0.8463 0.7408 0.8796 0.737 0.7395 0.7595 0.613 0.652 0.8028 0.7009 0.9171 0.9499 0.4776 0.6093 0.7906
Nuclear Periphery 0.0061 0.0004 0.0015 0.0006 0.0032 0.0005 0.0106 0.0009 0.0008 0.0026 0.0049 0.0009 0.0045 0.0006 0.0002 0.0039 0.001 0.0001
Nucleolus 0.001 0.0007 0.0013 0.0008 0.0029 0.0007 0.0022 0.0003 0.0009 0.0018 0.0023 0.0004 0.0026 0.0019 0.0002 0.0002 0.0001 0.0001
Nucleus 0.0052 0.0016 0.0028 0.0027 0.0046 0.0028 0.0038 0.0009 0.0016 0.0029 0.0083 0.0026 0.0027 0.0009 0.0003 0.0007 0.0004 0.0003
Peroxisomes 0.013 0.0005 0.0026 0.0076 0.021 0.0068 0.0023 0.0001 0.004 0.0013 0.007 0.0008 0.0142 0.0009 0.0001 0.1024 0.0549 0
Punctate Nuclear 0.0057 0.0047 0.0047 0.0027 0.0285 0.0035 0.0121 0.0003 0.0043 0.0006 0.0103 0.0014 0.025 0.0003 0.0002 0.0012 0.0009 0.0003
Vacuole 0.0037 0.0009 0.0049 0.0072 0.0034 0.0022 0.0538 0.03 0.0227 0.0913 0.0213 0.0159 0.012 0.0061 0.0012 0.0187 0.0007 0.0065
Vacuole Periphery 0.0008 0.0001 0.0005 0.0008 0.0017 0.0003 0.0061 0.0022 0.0017 0.0192 0.0089 0.0016 0.001 0.0011 0.0001 0.0307 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.0579 21.1151 29.0983 41.532 32.8122 11.2415 32.5325 34.3082 34.0263 57.5263
Translational Efficiency 0.4406 0.7678 0.7439 0.5713 0.7594 0.9854 0.8646 0.6029 0.8833 0.6688

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Zn2+-dependent endopolyphosphatase; required with PPN1 to mobilize polyphosphate stores in response to phosphate starvation; member of the PPP-superfamily of metalloproteases; localizes to the vacuolar lumen via the MVB pathway; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate
Localization
Cell Percentages vacuole (30%), cytoplasm (21%), mixed (43%)
Cell Cycle Regulation Yes
cytoplasm - MA (1.9e-07)
Subcompartmental Group N/A

Ppn2

Ppn2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ppn2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available