Standard name
Human Ortholog
Description Glycosylphosphatidylinositol-dependent cell wall protein; expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0 0 0
Cytoplasm 0.33 0.38 0.39 0.39 0.35 0.42 0.36 0.47 0.44 0.5 0.48 0.17 0.25 0.2 0.24 0.32 0.32 0 0.06 0 0 0.05 0.06
Endoplasmic Reticulum 0.36 0.3 0.22 0.36 0.56 0.53 0.42 0.33 0.27 0.22 0.18 0.83 0.66 0.68 0 0 0 0.48 0.39 0.34 0.36 0.25 0.12
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.08 0.08 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.36 0.35 0.35 0.25 0.12 0.09 0.23 0.21 0.28 0.3 0.31 0.09 0.11 0.07 0.71 0.66 0.61 0.37 0.47 0.48 0.48 0.56 0.63
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 4 5 8 16 14 8 0 13 0 0 2 0 0 1 2 1 0 0 0 1
Bud 2 0 0 1 0 5 9 6 8 7 11 0 0 1 1 0 0 12 7 2 2 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 2 2 0 4 9 10 13 13 10 5 12 1 2 5 0 0 3 0 0 0 0 0 0
Cytoplasm 106 96 34 77 88 128 183 255 269 137 183 4 14 9 27 46 39 17 17 5 1 3 5
Endoplasmic Reticulum 113 76 19 72 141 163 214 179 162 60 69 19 37 30 1 4 2 199 116 46 11 16 10
Endosome 0 0 1 3 1 1 0 2 3 1 0 0 0 0 1 2 0 5 6 3 0 0 4
Golgi 5 0 0 1 0 0 2 1 3 1 1 0 0 0 2 3 6 0 0 0 0 0 0
Mitochondria 0 0 3 8 0 1 1 5 4 7 7 0 0 0 0 4 0 1 0 0 0 0 1
Nucleus 5 6 3 1 1 1 0 4 12 3 15 0 0 0 16 12 10 4 3 1 0 1 1
Nuclear Periphery 0 0 0 1 1 1 8 4 8 3 1 0 1 0 1 0 0 8 4 5 0 0 0
Nucleolus 0 0 0 2 0 1 6 1 3 3 4 0 0 0 1 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 113 88 31 49 30 26 120 115 168 82 118 2 6 3 81 94 73 153 141 64 15 35 56
Unique Cell Count 317 250 88 199 252 305 513 541 607 275 379 23 56 44 114 142 120 413 303 135 33 63 90
Labelled Cell Count 346 269 92 223 276 345 572 600 658 309 435 26 60 50 131 165 136 413 303 135 33 63 90


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.7 19.3 10.6 15.2 19.4 17.0 15.6 15.8 16.6 15.7 15.8 20.9 20.4 19.3 27.8 26.6 27.8 17.3 12.8
Std Deviation (1e-4) 5.9 4.9 3.2 5.8 5.7 4.4 4.1 4.7 4.6 5.1 5.0 6.5 8.0 5.3 12.0 12.0 14.0 5.7 4.4
Intensity Change (Log2) 0.52 0.87 0.68 0.55 0.57 0.64 0.56 0.57 0.98 0.94 0.86 1.39 1.32 1.39 0.7 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.6 0.6 -0.5 1.5 1.0 1.8 1.6 -1.9 -1.7 -2.1 -2.3 -1.0 -0.9
Endoplasmic Reticulum 2.4 5.6 5.3 3.6 2.2 1.0 0 -0.7 5.5 5.3 5.2 -4.9 -4.6 -4.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 2.6 1.5 1.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.8 -4.9 -6.3 -2.4 -2.9 -1.5 -1.0 -0.7 -2.5 -3.3 -3.5 5.1 4.6 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.2622 31.7689 26.4682 17.0385 15.0271 26.697 19.9046 31.2487 29.1593 25.6118 29.6325 31.6973 26.5992 30.3375 37.5042 30.1002 20.4249 35.3483
Actin 0.0324 0.0004 0.0218 0.0297 0.0829 0.0158 0.0073 0.0002 0.0148 0.0195 0.0018 0.0002 0.0165 0.0002 0.052 0.0048 0.0917 0.0008
Bud 0.0008 0.0003 0.002 0.0009 0.0022 0.001 0.0011 0.0002 0.0026 0.0012 0.0008 0.0001 0.0004 0.0004 0.0008 0.0004 0.0014 0.0016
Bud Neck 0.0033 0.0021 0.0025 0.0009 0.0099 0.0038 0.0007 0.0008 0.0018 0.0062 0.0117 0.0015 0.0147 0.001 0.0024 0.0005 0.0047 0.002
Bud Periphery 0.0017 0.001 0.006 0.0019 0.0031 0.0023 0.0014 0.0007 0.0018 0.0039 0.001 0.0004 0.0006 0.001 0.001 0.0008 0.0025 0.0019
Bud Site 0.0141 0.0006 0.0237 0.0015 0.058 0.0009 0.0102 0.0006 0.0046 0.0014 0.0004 0.0001 0.0053 0.0011 0.0009 0.0002 0.0013 0.0001
Cell Periphery 0.0437 0.0521 0.0233 0.0203 0.0267 0.0046 0.0187 0.032 0.0195 0.0534 0.0303 0.0031 0.0115 0.0472 0.0184 0.0007 0.0029 0.0012
Cytoplasm 0.049 0.0575 0.0762 0.0685 0.031 0.0871 0.0536 0.0923 0.1593 0.0566 0.0732 0.0323 0.0255 0.0629 0.0653 0.0135 0.2154 0.0864
Cytoplasmic Foci 0.0251 0.0015 0.0136 0.0205 0.0146 0.0145 0.0476 0.0011 0.0095 0.0022 0.0032 0.0007 0.0185 0.0014 0.0036 0.0016 0.0047 0.0012
Eisosomes 0.0005 0.0001 0.0003 0.0003 0.0012 0.0001 0.0002 0 0.0001 0.0003 0 0 0.0006 0.0001 0.0004 0 0.0003 0
Endoplasmic Reticulum 0.2766 0.5557 0.529 0.3784 0.2915 0.5287 0.3056 0.6401 0.5522 0.3643 0.5343 0.7213 0.3535 0.5003 0.6455 0.6684 0.1172 0.5906
Endosome 0.0367 0.0092 0.026 0.0995 0.0411 0.0261 0.0803 0.0051 0.0232 0.0422 0.0477 0.0231 0.0362 0.0142 0.0064 0.0438 0.0395 0.0129
Golgi 0.0048 0.0018 0.0052 0.0406 0.0213 0.0089 0.0122 0.0003 0.0043 0.008 0.0039 0.0019 0.0033 0.0009 0.0035 0.0269 0.0105 0.003
Lipid Particles 0.024 0.0256 0.0109 0.0334 0.0723 0.0124 0.0297 0.0135 0.0067 0.0123 0.0047 0.0017 0.0084 0.0285 0.0058 0.0025 0.0044 0.0135
Mitochondria 0.006 0.0006 0.0046 0.0431 0.0162 0.0061 0.0147 0.0001 0.0061 0.0189 0.0014 0.0007 0.0042 0.0005 0.0073 0.018 0.0163 0.0021
None 0.0086 0.0004 0.0047 0.0025 0.0944 0.0015 0.0074 0.0001 0.0022 0.0014 0.0011 0.0001 0.02 0.0003 0.0008 0.0001 0.003 0.0002
Nuclear Periphery 0.0987 0.0469 0.0929 0.0642 0.0891 0.1267 0.1299 0.0386 0.0653 0.1367 0.0911 0.0735 0.2088 0.0388 0.078 0.0696 0.1382 0.1904
Nucleolus 0.0044 0.0009 0.0011 0.0008 0.0057 0.0006 0.003 0.0004 0.001 0.001 0.0002 0.0001 0.0011 0.003 0.0001 0.0001 0.0012 0.0007
Nucleus 0.04 0.012 0.0131 0.0076 0.0155 0.0137 0.037 0.0037 0.0246 0.0135 0.0154 0.0039 0.0427 0.0074 0.0062 0.0046 0.0338 0.0208
Peroxisomes 0.0054 0.0001 0.0016 0.0069 0.0022 0.0013 0.0046 0 0.0012 0.0003 0.0001 0 0.001 0 0.0022 0.0002 0.0008 0.0001
Punctate Nuclear 0.0245 0.0003 0.0157 0.0068 0.0146 0.0048 0.0175 0.0002 0.0046 0.0023 0.0015 0.0002 0.0088 0.0003 0.0012 0.0002 0.0098 0.0007
Vacuole 0.2654 0.2173 0.1156 0.1551 0.0916 0.1189 0.1945 0.1589 0.0834 0.2326 0.163 0.1243 0.1899 0.2729 0.0939 0.1212 0.282 0.0366
Vacuole Periphery 0.0343 0.0137 0.0099 0.0165 0.015 0.02 0.0229 0.0108 0.0113 0.0219 0.0133 0.0106 0.0287 0.0176 0.0045 0.0218 0.0183 0.0332

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 642.7599 150.0313 18.9497 34.1666 370.385 306.9264 145.2413 79.5117 37.5246 434.3682
Translational Efficiency 1.9727 1.4878 2.1997 1.4043 2.4549 4.3242 1.7579 1.5758 2.3384 2.0156

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
874 1562 286 895 832 1762 1605 488 1706 3324 1891 1383

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1102.52 1580.63 1592.53 1457.18 1465.99 1505.34 1752.60 1430.31 1279.78 1540.72 1728.39 1447.70
Standard Deviation 299.18 429.99 319.08 436.16 375.96 340.04 366.07 352.19 384.44 386.76 363.91 408.71
Intensity Change Log 2 0.519695 0.530516 0.402374 0.038214 0.257622 -0.035547 0.264792 0.381131 0.168884

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000991 0.000510 0.000470 0.001198 0.000270 0.000328 0.000320 0.001187 0.000640 0.000414 0.000342 0.001194
Bud Neck 0.002483 0.001954 0.006549 0.008113 0.001181 0.001123 0.001179 0.007532 0.001849 0.001513 0.001991 0.007908
Bud Site 0.023914 0.012605 0.014413 0.034058 0.010291 0.006646 0.005710 0.048062 0.017270 0.009446 0.007026 0.038999
Cell Periphery 0.013689 0.009117 0.005201 0.014425 0.014583 0.006363 0.007501 0.023393 0.014125 0.007657 0.007153 0.017589
Cytoplasm 0.055856 0.029503 0.031529 0.109626* 0.036953 0.018104 0.018406 0.098606 0.046637 0.023460 0.020391 0.105737*
Cytoplasmic Foci 0.009045 0.003919 0.003653 0.015839 0.003520 0.004541 0.000904 0.017764 0.006351 0.004248 0.001320 0.016519
Eisosomes 0.000062 0.000031 0.000026 0.000092 0.000017 0.000021 0.000031 0.000172 0.000040 0.000026 0.000030 0.000120
Endoplasmic Reticulum 0.512363* 0.559759* 0.580809* 0.290909* 0.565324* 0.644012* 0.820680* 0.376344* 0.538192* 0.604420* 0.784401* 0.321055*
Endosome 0.022944 0.020607 0.034398 0.039819 0.015796 0.019160 0.015939 0.040677 0.019458 0.019840 0.018731 0.040122
Golgi 0.003386 0.003321 0.001846 0.006645 0.002579 0.003200 0.001769 0.008518 0.002992 0.003257 0.001781 0.007306
Lipid Particles 0.007098 0.002414 0.008976 0.024610 0.004679 0.002767 0.007346 0.041298 0.005918 0.002602 0.007592 0.030498
Mitochondria 0.001340 0.001819 0.000283 0.001412 0.000494 0.001398 0.000441 0.000680 0.000928 0.001596 0.000417 0.001154
Mitotic Spindle 0.000149 0.000188 0.000312 0.008295 0.000292 0.000192 0.000689 0.004914 0.000219 0.000190 0.000632 0.007102
None 0.001606 0.002122 0.000041 0.001817 0.000556 0.000656 0.000944 0.000214 0.001094 0.001345 0.000807 0.001252
Nuclear Periphery 0.000660 0.002971 0.006835 0.003159 0.001158 0.001778 0.002047 0.000598 0.000903 0.002339 0.002772 0.002255
Nuclear Periphery Foci 0.003248 0.008142 0.030559 0.032617 0.007471 0.004116 0.012216 0.013349 0.005307 0.006008 0.014990 0.025818
Nucleolus 0.000214 0.000389 0.000500 0.000284 0.000121 0.000337 0.000033 0.000117 0.000169 0.000361 0.000103 0.000225
Nucleus 0.001947 0.002629 0.003670 0.005556 0.000906 0.001104 0.000537 0.004477 0.001439 0.001821 0.001011 0.005175
Peroxisomes 0.000461 0.000144 0.000301 0.000388 0.000121 0.000228 0.000066 0.000600 0.000295 0.000189 0.000101 0.000463
Vacuole 0.295552* 0.296407* 0.252290* 0.383746* 0.302178* 0.255645* 0.098575 0.302098* 0.298783* 0.274800* 0.121823 0.354936*
Vacuole Periphery 0.042991 0.041448 0.017342 0.017392 0.031511 0.028282 0.004668 0.009400 0.037392 0.034469 0.006585 0.014572

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.31 1.16 -0.85 -3.26 -2.69 -0.34 -0.74 -5.11 -4.17 -4.47 1.13 1.35 -2.84 -4.94 -5.21
Bud Neck 0.90 -2.69 -7.63 -9.08 -1.24 0.30 -0.25 -7.87 -8.01 -7.79 1.08 -0.66 -11.17 -12.41 -10.52
Bud Site 3.26 1.65 -3.10 -6.69 -4.08 1.95 1.95 -6.89 -7.77 -7.75 3.95 4.53 -7.35 -10.68 -10.89
Cell Periphery 2.40 3.57 0.24 -2.52 -3.70 4.54 3.83 -2.25 -5.61 -5.14 4.92 5.08 -1.14 -6.08 -6.20
Cytoplasm 4.94 2.56 -8.23 -13.15 -9.44 4.17 3.87 -7.40 -10.39 -10.23 6.61 6.82 -11.48 -17.22 -17.23
Cytoplasmic Foci 4.31 3.97 -4.18 -8.24 -7.80 -1.35 4.75 -6.44 -5.94 -7.77 2.88 8.06 -7.98 -10.09 -12.97
Eisosomes 2.05 2.76 -0.85 -1.98 -2.24 -1.06 -2.12 -2.82 -2.75 -2.52 1.91 1.11 -2.86 -3.46 -3.23
Endoplasmic Reticulum -2.75 -2.06 14.25 19.67 11.80 -5.02 -16.51 9.81 15.10 24.53 -5.74 -20.03 18.09 26.53 39.68*
Endosome 1.20 -2.92 -6.38 -8.56 -0.88 -2.44 -0.33 -6.67 -5.81 -6.61 -0.38 0.25 -9.93 -10.51 -10.07
Golgi 0.04 1.63 -2.40 -3.03 -5.14 -0.84 1.89 -3.91 -3.15 -4.61 -0.46 2.33 -4.58 -4.31 -7.21
Lipid Particles 3.16 -0.68 -6.47 -9.59 -4.04 1.45 -1.77 -7.54 -8.23 -6.98 3.32 -1.41 -10.11 -12.47 -9.19
Mitochondria -0.98 5.27 -0.11 0.74 -2.76 -2.61 0.50 -0.65 1.75 -0.82 -2.24 4.50 -0.72 1.21 -2.60
Mitotic Spindle -0.66 -1.80 -4.95 -4.93 -4.85 0.52 -0.64 -2.19 -2.24 -2.00 0.27 -0.84 -5.33 -5.36 -4.89
None -0.71 3.20 -0.12 0.54 -2.93 -0.26 -0.73 1.30 1.66 1.58 -0.61 0.62 -0.17 0.36 -0.70
Nuclear Periphery -5.49 -6.60 -5.41 -0.33 3.59 -1.46 -2.29 1.91 3.53 4.79 -4.87 -5.83 -4.15 0.19 1.34
Nuclear Periphery Foci -5.03 -7.39 -10.06 -8.12 -0.55 2.34 -2.63 -2.93 -6.23 -0.38 -0.88 -6.81 -9.88 -9.86 -4.61
Nucleolus -1.69 -0.86 -2.13 0.86 0.60 -1.70 7.76 -0.09 1.68 -5.21 -2.32 1.30 -2.60 1.49 -2.41
Nucleus -1.36 -1.56 -6.15 -4.76 -1.94 -0.63 1.95 -4.38 -4.26 -4.72 -1.34 1.19 -7.77 -6.93 -7.93
Peroxisomes 3.50 0.93 0.75 -3.37 -0.53 -1.65 2.61 -3.59 -2.38 -4.03 1.67 3.93 -2.23 -3.61 -5.51
Vacuole -0.17 1.80 -6.33 -6.98 -6.40 3.52 16.28 -0.86 -3.85 -14.02 2.51 18.55 -5.71 -8.80 -23.55
Vacuole Periphery 0.45 6.40 8.27 9.46 0.47 1.11 10.57 7.82 9.30 -2.63 1.25 15.07 10.88 12.47 -6.02
Standard name
Human Ortholog
Description Glycosylphosphatidylinositol-dependent cell wall protein; expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid
Localization
Cell Percentages ER (75%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Tos6

Tos6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tos6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available