Standard name
Human Ortholog
Description Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.13 0.09 0.1 0.06 0.06 0.11 0.14 0.08 0.17 0 0.06 0 0.11 0.05 0.15 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 0.05 0.1 0.1 0.1 0.06 0.06 0.1 0.11 0.09 0.09 0.1 0.17 0.21 0.18 0.16 0.14 0 0 0 0 0 0
Cytoplasm 0.05 0.08 0.11 0.09 0.09 0.14 0.14 0.14 0.13 0.16 0.19 0.16 0.05 0 0.05 0.21 0.2 0.22 0 0 0 0 0 0
Endoplasmic Reticulum 0.95 0.91 0.89 0.86 0.84 0.78 0.8 0.74 0.76 0.69 0.59 0.63 0.91 0.92 0.86 0 0 0 0.9 0.91 0.87 0.89 0.89 0.89
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.1 0.07 0 0 0 0 0 0 0 0.06 0 0 0.37 0.53 0.41 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.09 0 0.17 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.07 0 0.06 0 0.07 0 0 0 0.21 0.37 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 4 0 25 20 23 19 17 27 33 14 39 6 17 10 34 21 44 1 1 0 2 6 9
Bud 0 1 0 0 1 0 0 4 2 6 0 2 0 0 0 2 4 6 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 8 1 19 22 22 18 18 23 26 15 21 31 53 70 56 65 43 1 0 0 5 15 18
Cytoplasm 5 10 2 17 20 32 42 41 30 38 33 37 17 8 17 67 82 66 3 0 0 1 3 4
Endoplasmic Reticulum 95 121 17 169 190 174 240 217 178 164 103 146 284 279 292 2 7 2 101 135 53 166 317 391
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 1 0
Golgi 0 0 0 20 17 8 1 7 3 5 0 3 19 8 12 115 221 125 0 1 1 2 1 1
Mitochondria 0 0 0 1 0 3 10 3 20 8 29 19 0 0 0 7 8 12 0 1 1 3 3 3
Nucleus 0 0 0 0 3 0 2 0 0 1 0 2 0 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 5 0 0 4 1 6 6 6 12 6 4 2 0 1 4 4 1 4 1 0 1 1 0 1
Nucleolus 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 1 1 0 6 3 4 10 21 7 15 5 15 5 2 10 65 153 31 0 4 1 1 3 2
Unique Cell Count 100 133 19 197 227 222 299 292 235 236 175 230 311 304 341 315 417 303 113 148 62 186 358 440
Labelled Cell Count 110 145 20 261 277 272 348 335 303 302 203 286 362 368 415 355 563 334 113 148 62 186 358 440


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.7 26.9 19.2 21.3 20.1 17.9 16.6 16.5 15.9 16.1 15.2 16.1 26.2 25.3 23.9 24.1 29.9 28.8 23.4 22.8 22.6
Std Deviation (1e-4) 3.0 5.1 2.9 4.0 3.9 3.5 3.3 3.9 3.5 3.4 3.3 3.2 4.7 3.8 4.0 5.5 6.7 5.9 3.9 3.8 3.7
Intensity Change (Log2) – – – 0.15 0.06 -0.11 -0.21 -0.22 -0.28 -0.25 -0.34 -0.26 0.45 0.39 0.31 0.33 0.63 0.58 0.28 0.25 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum -0.4 -0.7 -1.1 -1.0 -1.5 -1.4 -1.8 -2.6 -2.3 0.3 0.4 -0.5 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.3 4.5 3.6
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 29.7699 29.208 28.2257 29.0443 27.4975 27.3673 32.8649 32.5785 32.7828 28.8339 29.5816 31.6465 35.3657 33.6931 35.0871 33.0611 33.3877 32.7914
Actin 0.0005 0.0004 0 0 0 0 0.0017 0 0.0004 0 0.0091 0 0.0006 0.0012 0.0001 0 0.0001 0.0005
Bud 0 0 0 0 0 0 0.0001 0 0.0002 0 0 0 0 0.0001 0 0 0 0
Bud Neck 0.0001 0.0001 0 0 0 0.0001 0.0003 0 0 0 0.0001 0.0004 0.0001 0.0007 0.0001 0.0001 0.0002 0.0008
Bud Periphery 0.0001 0.0001 0 0 0 0 0.0002 0 0.0012 0 0.0003 0 0.0002 0.0001 0 0 0 0.0001
Bud Site 0.0001 0 0 0 0 0 0.0006 0 0 0 0.0001 0 0 0.0003 0 0 0 0
Cell Periphery 0.0087 0.0204 0.0078 0.0123 0.0027 0.0035 0.0105 0.0098 0.0055 0.0039 0.0094 0.0048 0.0065 0.0069 0.0057 0.0013 0.0023 0.002
Cytoplasm 0.0051 0.0101 0.0017 0.0002 0.0004 0.0006 0.0018 0.0047 0.0006 0.0002 0.0007 0.0014 0.0026 0.0062 0.0019 0.0004 0.0007 0.0024
Cytoplasmic Foci 0.0004 0.0002 0 0 0 0 0.0012 0.0001 0.0021 0 0.0108 0 0 0.0001 0.0001 0 0 0.0026
Eisosomes 0.0028 0.0002 0.0002 0.0002 0.0001 0 0.0005 0 0.0001 0 0.0009 0.0001 0.0001 0.0001 0 0 0.0001 0
Endoplasmic Reticulum 0.9479 0.9447 0.9814 0.9824 0.9921 0.9917 0.94 0.9601 0.9733 0.9919 0.9205 0.9874 0.9294 0.9674 0.9775 0.9925 0.9828 0.9725
Endosome 0.0014 0.0007 0.0001 0 0.0001 0 0.0024 0.0003 0.0002 0 0.0028 0.0002 0.0005 0.0002 0.0002 0.0002 0.0003 0.0022
Golgi 0.0013 0.0007 0.0004 0.0001 0.0004 0.0001 0.0006 0.0014 0.0004 0 0.0057 0.0003 0.0004 0.0005 0.0009 0.0005 0.0013 0.0007
Lipid Particles 0.0029 0.014 0.0036 0.0011 0.0002 0.0005 0.0026 0.0188 0.0024 0.0003 0.0125 0.0006 0.0013 0.0019 0.0048 0.0001 0.0003 0.0013
Mitochondria 0.0011 0.0002 0 0 0 0 0.0005 0 0.004 0 0.0003 0 0.0008 0 0 0 0 0.0002
None 0.0004 0 0 0 0 0 0.0006 0 0.0001 0 0.0008 0 0.0001 0 0 0 0 0.0003
Nuclear Periphery 0.0203 0.0047 0.0033 0.003 0.0033 0.0025 0.027 0.0032 0.0058 0.0032 0.0043 0.0035 0.0505 0.0115 0.0073 0.0037 0.0099 0.0108
Nucleolus 0.0001 0 0 0 0 0 0.0003 0 0 0 0.0001 0 0 0 0 0 0 0.0002
Nucleus 0.0009 0.0001 0 0 0 0 0.0013 0 0.0001 0 0.0001 0.0001 0.0011 0.0003 0.0001 0 0.0002 0.0006
Peroxisomes 0 0 0 0 0 0 0.0002 0 0.0015 0 0.0139 0 0 0 0 0 0 0.0001
Punctate Nuclear 0.0001 0 0 0 0 0 0.002 0 0.0001 0 0.0063 0 0 0 0 0 0 0.0011
Vacuole 0.0041 0.0031 0.0012 0.0005 0.0006 0.0008 0.0038 0.0014 0.0017 0.0005 0.001 0.001 0.004 0.0023 0.0011 0.0009 0.0014 0.0012
Vacuole Periphery 0.0016 0.0001 0.0001 0 0.0001 0 0.0016 0 0.0004 0 0.0002 0.0001 0.0017 0.0002 0.0002 0.0001 0.0004 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 156.3742 112.0406 95.571 118.7048 126.8112 105.3624 121.4893 115.046 102.7617 146.7808
Translational Efficiency 1.0233 0.9627 1.171 0.6406 1.0631 1.756 1.0213 1.0172 1.2036 1.028

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1555 1216 1697 1244 1732 1689 244 1443 3287 2905 1941 2687

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1609.70 2059.86 2549.25 2625.35 1653.38 1794.07 2609.90 2583.15 1632.72 1905.33 2556.87 2602.69
Standard Deviation 292.61 372.00 398.57 504.95 279.18 348.14 353.02 495.12 286.44 381.56 393.65 500.14
Intensity Change Log 2 — 0.355754 0.663281 0.705718 — 0.117818 0.658576 0.643713 — 0.240096 0.660899 0.674634

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000520 0.000981 0.000196 0.001689 0.000444 0.000341 0.000145 0.000935 0.000480 0.000609 0.000190 0.001284
Bud Neck 0.000293 0.000241 0.000563 0.002929 0.000079 0.000225 0.002556 0.001589 0.000180 0.000232 0.000813 0.002209
Bud Site 0.003462 0.002095 0.000753 0.010844 0.000581 0.001782 0.001355 0.005710 0.001944 0.001913 0.000829 0.008087
Cell Periphery 0.004570 0.003917 0.007834 0.032847 0.003374 0.003586 0.012786 0.037915 0.003940 0.003725 0.008457 0.035569
Cytoplasm 0.002790 0.001026 0.000289 0.006761 0.000399 0.002611 0.000214 0.002308 0.001530 0.001947 0.000279 0.004370
Cytoplasmic Foci 0.003752 0.000957 0.000034 0.001196 0.000587 0.002201 0.000954 0.001267 0.002084 0.001681 0.000149 0.001234
Eisosomes 0.000182 0.000161 0.000281 0.000368 0.000069 0.000191 0.000282 0.000218 0.000122 0.000179 0.000281 0.000288
Endoplasmic Reticulum 0.960607* 0.964949* 0.970746* 0.828409* 0.985444* 0.948326* 0.924315* 0.868843* 0.973694* 0.955284* 0.964909* 0.850124*
Endosome 0.007390 0.006238 0.002507 0.010491 0.002807 0.009611 0.008993 0.007612 0.004975 0.008199 0.003322 0.008945
Golgi 0.004587 0.005754 0.002400 0.013318 0.001465 0.006647 0.007319 0.009198 0.002942 0.006273 0.003018 0.011106
Lipid Particles 0.004568 0.004736 0.002408 0.037145 0.001600 0.009981 0.009346 0.028241 0.003004 0.007786 0.003280 0.032363
Mitochondria 0.000327 0.000514 0.000196 0.000723 0.000137 0.000510 0.000112 0.000587 0.000227 0.000512 0.000185 0.000650
Mitotic Spindle 0.000111 0.000058 0.000026 0.001016 0.000052 0.000181 0.000088 0.001058 0.000080 0.000130 0.000033 0.001039
None 0.000873 0.000575 0.000264 0.000671 0.000290 0.001016 0.000011 0.000525 0.000566 0.000831 0.000232 0.000592
Nuclear Periphery 0.000297 0.000557 0.002002 0.003988 0.000307 0.000800 0.004902 0.003633 0.000302 0.000698 0.002366 0.003797
Nuclear Periphery Foci 0.002145 0.001987 0.001935 0.012164 0.000644 0.004436 0.012745 0.004818 0.001354 0.003411 0.003294 0.008219
Nucleolus 0.000004 0.000004 0.000005 0.000026 0.000002 0.000102 0.000004 0.000061 0.000003 0.000061 0.000005 0.000045
Nucleus 0.000086 0.000046 0.000042 0.000447 0.000036 0.000166 0.000059 0.000559 0.000060 0.000116 0.000044 0.000507
Peroxisomes 0.000100 0.000064 0.000007 0.000243 0.000086 0.000130 0.000010 0.000124 0.000093 0.000103 0.000008 0.000179
Vacuole 0.002262 0.003200 0.006285 0.029807 0.001129 0.005517 0.012356 0.021008 0.001665 0.004547 0.007049 0.025082
Vacuole Periphery 0.001076 0.001939 0.001228 0.004917 0.000468 0.001641 0.001446 0.003793 0.000755 0.001765 0.001256 0.004313

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.89 2.54 -2.26 -1.07 -2.85 0.27 0.79 -1.40 -2.07 -2.54 -0.46 1.39 -2.57 -2.15 -3.75
Bud Neck 0.57 -2.18 -5.59 -5.68 -5.09 -4.15 -3.77 -6.70 -6.07 1.27 -1.13 -4.86 -7.83 -7.67 -5.38
Bud Site 1.37 3.12 -4.28 -5.03 -5.87 -2.34 -1.00 -4.60 -3.44 -3.32 0.04 2.05 -6.11 -6.10 -7.04
Cell Periphery 0.78 -3.29 -11.51 -11.70 -9.84 -0.36 -2.72 -13.80 -13.78 -6.01 0.43 -4.95 -17.95 -18.11 -14.10
Cytoplasm 2.39 3.59 -3.31 -4.89 -5.50 -5.31 1.08 -4.48 -0.41 -4.67 -1.02 3.73 -4.72 -4.33 -6.95
Cytoplasmic Foci 3.28 4.57 2.89 -0.88 -4.75 -3.81 -0.44 -1.84 1.80 -0.42 0.84 4.69 1.73 1.22 -4.81
Eisosomes 0.21 -0.81 -1.46 -1.91 -0.64 -1.14 -2.65 -5.57 -0.28 0.73 -0.74 -1.76 -2.74 -1.38 -0.08
Endoplasmic Reticulum -0.91 -2.42 16.48 16.93 18.22 9.91 5.38 18.67 11.55 4.39 6.30 3.20 24.52 19.93 21.52
Endosome 1.47 8.20 -3.70 -5.09 -11.05 -11.46 -3.69 -9.49 2.63 0.83 -6.67 4.04 -8.11 -1.56 -11.49
Golgi -0.97 2.86 -6.60 -5.05 -9.65 -5.39 -1.66 -8.73 -2.11 -0.54 -4.33 -0.14 -10.49 -5.07 -9.75
Lipid Particles -0.13 2.07 -9.88 -9.80 -10.80 -5.69 -2.26 -11.51 -6.83 -4.38 -4.62 -0.54 -14.87 -11.63 -14.46
Mitochondria -0.93 1.76 -2.40 -0.82 -3.13 -2.80 0.51 -5.06 -0.74 -5.10 -2.43 0.71 -4.70 -1.12 -4.94
Mitotic Spindle 1.40 2.36 -3.17 -3.32 -3.40 -1.14 -1.03 -2.84 -2.27 -2.73 -0.82 1.96 -4.24 -3.84 -4.41
None 0.79 2.48 0.59 -0.34 -2.04 -2.29 2.09 -1.51 1.53 -5.07 -1.10 2.15 -0.32 0.93 -2.83
Nuclear Periphery -2.04 -10.20 -14.71 -12.31 -6.66 -1.87 -5.47 -14.34 -8.18 1.52 -2.45 -11.34 -20.53 -13.38 -5.75
Nuclear Periphery Foci 0.23 0.19 -6.52 -6.45 -6.49 -5.07 -3.67 -7.45 -0.48 2.39 -3.92 -3.20 -8.74 -5.55 -5.18
Nucleolus 0.40 -1.24 -2.91 -2.95 -2.81 -1.34 -2.33 -1.59 0.54 -1.54 -1.33 -3.13 -2.22 0.32 -2.13
Nucleus 1.76 2.01 -3.87 -4.12 -4.15 -1.62 -1.25 -2.21 -1.60 -2.12 -1.25 1.06 -3.70 -3.15 -3.78
Peroxisomes 1.51 4.89 -1.09 -1.37 -1.82 -1.10 3.73 -1.29 0.33 -5.09 -0.51 6.29 -1.37 -1.06 -2.82
Vacuole -1.81 -5.83 -13.01 -12.42 -10.74 -4.82 -4.11 -11.95 -8.71 -2.90 -5.09 -7.71 -17.62 -14.82 -12.42
Vacuole Periphery -1.67 -0.61 -5.70 -3.77 -5.43 -4.29 -1.92 -6.12 -3.79 -3.29 -3.74 -2.24 -8.33 -5.43 -6.70
Standard name
Human Ortholog
Description Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Kre1

Kre1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kre1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available