Standard name
Human Ortholog
Description Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.05
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0.06 0 0 0.08 0.08 0.15 0 0 0 0 0 0
Cytoplasm 0 0 – 0.05 0.05 0.06 0.07 – 0 0 0 0 0 0 0 0.36 0.31 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0.06 0 0.07 0 0 0
Golgi 0.1 0 – 0.05 0.35 0 0 – 0 0 0 0 0.19 0.14 0.25 0.31 0.38 0.25 0.06 0.08 0 0.07 0.07 0.1
Mitochondria 0.78 0.93 – 0.87 0.65 0.86 0.69 – 0.79 0.85 0.9 0.87 0.87 0.88 0.77 0.13 0.1 0 0.73 0.82 0.81 0.74 0.74 0.67
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0.08 – 0.07 0 0 0.07 – 0.08 0 0.06 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleolus 0.08 0 – 0 0 0.08 0.12 – 0.12 0.12 0.16 0.06 0.05 0 0 0 0 0.07 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.23 0.14 – 0.05 0.31 0.14 0.16 – 0.08 0.1 0.09 0.08 0.09 0.12 0.14 0.24 0.32 0.3 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 2 0 1 0 – 0 0 0 0 1 1 4 0 1 2 0 1 0 4 9 22
Bud 0 2 – 2 0 1 2 – 3 2 1 1 0 5 2 0 0 0 1 0 0 0 2 2
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 – 0 0 0 0 – 0 2 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 1 – 1 2 0 3 – 2 0 2 1 8 7 15 8 6 6 0 0 0 0 0 0
Cytoplasm 4 1 – 4 7 4 13 – 7 4 4 2 4 9 12 35 22 8 0 0 0 0 0 2
Endoplasmic Reticulum 0 3 – 0 3 0 1 – 1 0 0 0 2 6 9 3 1 1 0 0 0 1 1 3
Endosome 3 1 – 0 5 0 4 – 0 1 0 0 1 7 9 5 2 1 6 1 1 8 9 15
Golgi 9 1 – 4 50 1 5 – 2 2 1 2 26 51 104 30 27 10 6 8 0 14 27 42
Mitochondria 72 84 – 66 93 57 131 – 126 125 121 97 120 313 327 13 7 1 79 87 13 143 284 288
Nucleus 0 0 – 1 3 0 8 – 3 1 4 1 0 1 0 4 5 1 0 0 0 0 2 0
Nuclear Periphery 6 7 – 5 2 1 13 – 13 6 8 8 5 6 13 0 0 2 0 0 0 1 4 4
Nucleolus 7 2 – 2 4 5 23 – 19 18 22 7 7 3 2 1 3 3 0 0 0 1 3 2
Peroxisomes 0 0 – 0 0 0 1 – 0 0 0 1 4 5 8 2 0 1 4 0 0 13 22 23
SpindlePole 1 0 – 0 1 0 2 – 2 3 1 0 0 1 0 1 0 0 0 0 0 0 3 4
Vac/Vac Membrane 21 13 – 4 45 9 31 – 12 14 12 9 13 44 60 24 23 12 2 4 0 5 12 11
Unique Cell Count 92 90 76 144 66 191 159 147 135 111 138 354 422 98 72 40 108 106 16 194 386 429
Labelled Cell Count 127 115 91 215 79 237 190 178 176 129 191 459 565 126 97 48 108 106 16 194 386 429


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.0 8.4 – 7.7 9.3 6.0 8.2 – 7.0 7.1 6.7 7.8 10.7 9.5 10.4 12.9 13.6 14.2 9.2 9.2 9.6
Std Deviation (1e-4) 1.9 2.0 – 1.7 1.6 1.4 1.6 – 1.6 1.7 1.4 1.9 2.4 2.1 1.9 3.9 4.5 3.6 2.3 2.3 2.6
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.2999 7.0497 6.5004 5.9604 6.0249 6.6111 8.102 9.0967 8.778 8.441 8.0558 8.416 9.7397 11.0165 9.933 10.0794 9.1418 9.8299
Actin 0.023 0.0035 0.0019 0.0005 0.0026 0.0083 0.0234 0.0048 0.0031 0.0006 0.0003 0.0186 0.004 0.0069 0.0078 0.0002 0.0009 0.0005
Bud 0.0006 0.0001 0 0 0.0001 0 0.0003 0.0005 0.0027 0.0001 0 0.0004 0.0001 0.0006 0.0009 0 0.0001 0.0001
Bud Neck 0.0004 0.0001 0 0 0 0.0001 0.0004 0.0004 0.0003 0 0 0.0009 0.0001 0.0006 0.0002 0 0 0.0001
Bud Periphery 0.0008 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0.0007 0.0044 0.0002 0 0.0006 0.0003 0.0012 0.0021 0.0001 0.0002 0.0002
Bud Site 0.0039 0.0001 0.0001 0 0.0001 0.0001 0.0007 0.0051 0.0088 0.0001 0 0.0004 0.0003 0.0032 0.0051 0 0 0.0001
Cell Periphery 0.0004 0.0002 0.0001 0.0001 0 0 0.0002 0.0009 0.0003 0.0001 0 0.0002 0.0001 0.0007 0.0009 0 0 0.0001
Cytoplasm 0.0004 0 0.0001 0 0 0.0014 0.0039 0.0001 0.0002 0 0 0.0004 0.0004 0.0001 0.0001 0 0.0001 0
Cytoplasmic Foci 0.0063 0.0011 0.0015 0.0004 0.0009 0.0007 0.0181 0.0065 0.0094 0.0009 0 0.005 0.0026 0.0041 0.0012 0.0001 0.0007 0.0005
Eisosomes 0.0008 0.0002 0.0002 0.0001 0.0001 0.0001 0.0003 0.0003 0.0001 0.0001 0.0001 0.0001 0.0003 0.0002 0.0003 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0009 0 0.0012 0 0 0.0002 0.0069 0.0001 0.0004 0.0002 0 0.0003 0.0002 0.0002 0.0001 0 0.0001 0.0001
Endosome 0.0169 0.0089 0.0032 0.0023 0.0022 0.0023 0.0325 0.0116 0.0146 0.0072 0.0001 0.0085 0.0095 0.0203 0.0047 0.0003 0.0389 0.005
Golgi 0.0209 0.0178 0.0056 0.003 0.0072 0.0057 0.0202 0.0203 0.0277 0.0043 0.0003 0.0069 0.0141 0.0224 0.0095 0.0006 0.016 0.0065
Lipid Particles 0.0262 0.0042 0.0028 0.0094 0.0043 0.0062 0.0265 0.0123 0.0082 0.0264 0.0001 0.0043 0.0448 0.0141 0.0039 0.004 0.0014 0.0099
Mitochondria 0.849 0.951 0.9645 0.9688 0.9797 0.9612 0.8241 0.9135 0.8935 0.9509 0.9978 0.9407 0.8881 0.8953 0.9429 0.9919 0.9295 0.9606
None 0.0032 0 0.0012 0 0 0.002 0.0011 0 0.0001 0.0001 0 0.0002 0.0002 0.0001 0.0001 0 0 0
Nuclear Periphery 0.0023 0 0.0023 0 0 0.0013 0.015 0.0001 0.0004 0.0019 0 0.0026 0.004 0.0002 0.0001 0 0.0001 0.0004
Nucleolus 0.0029 0.0011 0.0001 0.0003 0.0001 0.0003 0.0008 0.0005 0.0004 0.0003 0 0.0003 0.0006 0.0038 0.0002 0 0 0.0005
Nucleus 0.0012 0.0002 0.0001 0.0001 0 0.0014 0.0033 0.0001 0.0003 0.0002 0 0.0004 0.0091 0.0003 0.0001 0 0 0.0002
Peroxisomes 0.0242 0.005 0.0035 0.0011 0.0011 0.0021 0.0092 0.0086 0.0085 0.0011 0.0002 0.0027 0.0116 0.0121 0.0061 0.0011 0.0013 0.0014
Punctate Nuclear 0.0012 0 0.0001 0 0 0.0026 0.0032 0 0.0002 0.0003 0 0.0044 0.0008 0 0 0 0 0
Vacuole 0.0053 0.0021 0.0027 0.002 0.0003 0.0006 0.0038 0.0053 0.005 0.0014 0.0001 0.0009 0.0025 0.0059 0.0057 0.0002 0.0032 0.0048
Vacuole Periphery 0.0094 0.0042 0.0088 0.0118 0.0009 0.0034 0.0055 0.0083 0.0114 0.0036 0.0009 0.0012 0.0062 0.0078 0.008 0.0012 0.0072 0.0092

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 106.2538 96.4457 101.9285 104.3447 142.0015 109.4625 102.6874 98.7252 105.9305 101.0733
Translational Efficiency 1.2786 1.5758 1.3899 1.6083 1.2214 1.1337 1.2093 1.3505 1.1072 1.2301

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1904 615 2005 1141 1467 1741 740 1653 3371 2356 2745 2794

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 827.98 1067.29 1297.82 1314.56 836.83 1032.67 1016.34 1356.57 831.83 1041.71 1221.94 1339.41
Standard Deviation 195.12 199.41 229.34 216.50 115.68 161.35 234.84 234.67 165.37 172.77 262.46 228.36
Intensity Change Log 2 — 0.366284 0.648422 0.666912 — 0.303373 0.280377 0.696957 — 0.335004 0.475131 0.682093

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000754 0.008486 0.000642 0.007324 0.001544 0.001337 0.002544 0.002761 0.001097 0.003204 0.001155 0.004624
Bud Neck 0.001083 0.002760 0.001203 0.001367 0.000892 0.000798 0.022704 0.000832 0.000999 0.001310 0.006999 0.001051
Bud Site 0.001574 0.004538 0.002729 0.004337 0.003016 0.001713 0.046719 0.003011 0.002202 0.002450 0.014588 0.003553
Cell Periphery 0.000220 0.000801 0.000294 0.000490 0.000241 0.000189 0.004450 0.000733 0.000229 0.000349 0.001414 0.000634
Cytoplasm 0.000768 0.000557 0.000213 0.000842 0.000328 0.000373 0.015704 0.000365 0.000577 0.000421 0.004389 0.000560
Cytoplasmic Foci 0.006065 0.018394 0.008972 0.009888 0.009985 0.007922 0.029025 0.004670 0.007771 0.010655 0.014378 0.006801
Eisosomes 0.000029 0.000320 0.000094 0.000235 0.000045 0.000049 0.000226 0.000191 0.000036 0.000120 0.000130 0.000209
Endoplasmic Reticulum 0.000173 0.000622 0.000240 0.000404 0.000249 0.000166 0.001683 0.000255 0.000206 0.000285 0.000629 0.000316
Endosome 0.009208 0.017121 0.004400 0.006351 0.018627 0.012511 0.049707 0.003678 0.013307 0.013715 0.016614 0.004770
Golgi 0.011817 0.049042 0.032375 0.050913 0.017564 0.016664 0.034289 0.027691 0.014318 0.025116 0.032891 0.037174
Lipid Particles 0.003571 0.008990 0.002775 0.000811 0.003975 0.002243 0.009358 0.000492 0.003747 0.004004 0.004549 0.000622
Mitochondria 0.949381* 0.845794* 0.873032* 0.884353* 0.917724* 0.931973* 0.667694* 0.931778* 0.935605* 0.909477* 0.817677* 0.912411*
Mitotic Spindle 0.001121 0.002579 0.002724 0.003548 0.001152 0.002495 0.006431 0.002041 0.001134 0.002517 0.003723 0.002657
None 0.000439 0.000668 0.002289 0.000969 0.000289 0.000311 0.003661 0.000948 0.000373 0.000405 0.002659 0.000956
Nuclear Periphery 0.000076 0.000219 0.000928 0.000267 0.000127 0.000088 0.000824 0.000090 0.000098 0.000123 0.000900 0.000163
Nuclear Periphery Foci 0.000133 0.000216 0.000602 0.000167 0.000161 0.000143 0.001875 0.000132 0.000145 0.000162 0.000945 0.000146
Nucleolus 0.000222 0.000146 0.000448 0.000104 0.000095 0.000271 0.005447 0.000101 0.000167 0.000239 0.001795 0.000102
Nucleus 0.001155 0.000083 0.000113 0.000071 0.000084 0.000146 0.005063 0.000045 0.000689 0.000129 0.001448 0.000056
Peroxisomes 0.006948 0.025180 0.002506 0.006279 0.013385 0.006421 0.028746 0.003468 0.009749 0.011318 0.009580 0.004616
Vacuole 0.001659 0.006690 0.015946 0.007167 0.004174 0.006703 0.037419 0.005761 0.002753 0.006700 0.021735 0.006335
Vacuole Periphery 0.003604 0.006793 0.047476 0.014112 0.006344 0.007483 0.026432 0.010955 0.004797 0.007303 0.041803 0.012244

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.08 0.71 -5.49 0.60 -5.61 0.46 -1.86 -1.66 -2.09 -0.30 -4.42 -0.28 -5.49 -1.89 -5.52
Bud Neck -2.31 -0.26 -0.62 2.17 -0.55 0.33 -7.44 0.17 -0.24 7.47 -0.98 -6.98 -0.21 1.12 7.16
Bud Site -2.21 -1.39 -2.93 0.12 -1.46 1.33 -8.39 -0.10 -1.94 8.44 -0.37 -7.79 -2.11 -1.75 6.83
Cell Periphery -1.35 -0.65 -2.45 0.72 -1.60 0.88 -6.07 -3.85 -4.32 5.29 -0.97 -5.78 -4.48 -2.06 3.62
Cytoplasm 0.82 2.17 -0.12 -0.91 -2.14 -0.37 -5.28 -0.65 -0.07 5.26 0.98 -4.68 0.12 -0.98 4.74
Cytoplasmic Foci -6.12 -2.91 -2.78 3.74 -0.63 1.60 -6.22 4.51 3.26 8.24 -2.99 -5.87 1.15 3.88 6.58
Eisosomes -9.38 -8.86 -8.54 2.18 -5.62 -0.66 -10.24 -4.70 -4.57 1.00 -8.95 -12.64 -8.30 -3.95 -3.63
Endoplasmic Reticulum -2.86 -1.56 -3.28 1.29 -2.24 1.16 -7.94 -0.08 -2.03 8.32 -1.40 -6.82 -2.35 -0.56 5.02
Endosome -3.43 4.70 2.47 4.85 -2.45 3.22 -7.22 9.31 7.71 11.43 -0.31 -2.26 9.15 8.63 9.57
Golgi -7.29 -8.55 -9.16 -0.29 -3.99 0.40 -4.78 -3.39 -3.78 1.68 -5.42 -9.24 -9.38 -4.24 -1.49
Lipid Particles -2.58 0.90 3.51 4.17 4.33 1.57 -3.21 3.97 2.53 6.09 -0.28 -1.08 5.35 4.66 7.69
Mitochondria 9.11 12.14 8.48 -3.02 -1.32 -2.16 16.81 -2.11 0.08 -18.13* 5.01 18.53 4.88 -0.48 -14.11
Mitotic Spindle -1.66 -2.39 -3.52 -0.93 -0.93 -2.17 -4.91 -2.56 0.66 3.96 -2.67 -4.62 -4.29 -0.26 1.69
None -1.97 -14.45 -5.03 -3.94 13.66 -0.46 -4.53 -10.17 -14.02 3.65 -0.46 -10.45 -8.88 -14.98 8.00
Nuclear Periphery -2.57 -6.51 -2.29 -0.49 4.30 1.15 -3.80 1.08 -0.16 4.05 -1.07 -7.43 -1.71 -1.06 6.58
Nuclear Periphery Foci -1.17 -4.25 -0.45 1.18 4.55 0.47 -4.57 0.32 0.10 4.57 -0.36 -6.29 -0.03 0.28 6.17
Nucleolus 0.78 -1.51 1.21 1.21 3.22 -1.48 -3.84 -0.18 1.45 3.83 -0.67 -4.16 1.12 1.57 4.37
Nucleus 2.10 2.04 2.12 0.26 1.04 -0.93 -5.03 1.34 1.60 5.07 1.93 -1.81 2.18 1.51 5.14
Peroxisomes -4.59 4.00 0.45 4.71 -3.08 3.77 -3.97 5.65 2.83 7.07 -1.05 0.08 4.80 5.04 4.16
Vacuole -3.52 -12.47 -5.50 -0.29 5.91 -2.64 -10.47 -1.61 0.79 9.78 -5.39 -15.55 -5.19 0.35 11.42
Vacuole Periphery -2.90 -16.79 -7.51 -4.28 11.40 -1.11 -8.71 -3.98 -2.89 6.46 -3.48 -18.29 -8.65 -4.94 13.80
Standard name
Human Ortholog
Description Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Rcf2

Rcf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rcf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available