Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0.05 0 0 0 0 0.05 0.05
Bud 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0.07 0.15 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.13 0.52 0.27 0.19 0.08 0.09 0 0.08 0.13 0.13
Mitochondria 0.91 0.91 0.93 0.93 0.85 0.89 0.9 0.93 0.92 0.94 0.95 0.9 0.89 0.81 0.06 0 0.08 0.81 0.76 0.78 0.69 0.64 0.62
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0.07 0.06 0.09 0.13 0.07 0.13 0.07 0.12 0.08 0.08 0.05 0 0 0.08 0.06 0 0 0 0 0 0
Nucleolus 0.07 0 0.06 0.05 0.07 0.12 0.09 0.1 0.09 0.08 0.09 0 0 0 0 0 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0 0.08 0.06 0.07 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.09 0.05 0 0.06 0.05 0 0 0 0 0 0.11 0.07 0.11 0.19 0.31 0.21 0.05 0.05 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 0 1 0 0 0 1 0 0 0 2 2 12 0 3 2 4 3 3 12 15
Bud 3 0 7 17 3 4 13 14 14 12 14 4 1 4 4 2 2 0 0 0 1 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 1
Bud Site 0 0 3 5 2 0 7 3 5 1 6 0 0 0 0 1 0
Cell Periphery 7 6 4 11 2 1 1 1 5 7 4 9 12 7 7 1 3 0 0 0 0 1 0
Cytoplasm 0 2 9 11 20 11 15 13 8 9 7 9 9 23 8 4 9 0 0 1 1 0 4
Endoplasmic Reticulum 4 1 2 2 2 4 0 3 0 3 3 0 4 2 1 1 3 0 0 0 1 0 2
Endosome 3 0 0 1 15 0 7 0 3 0 0 2 7 9 5 1 2 7 4 9 2 11 9
Golgi 5 8 1 1 8 0 1 0 1 0 0 16 26 54 57 7 12 24 18 9 13 34 44
Mitochondria 273 186 223 267 318 344 517 392 514 397 477 233 347 347 7 0 5 240 166 188 114 177 211
Nucleus 2 0 2 1 1 1 6 0 0 2 1 0 0 0 2 0 3 1 0 0 1 2 2
Nuclear Periphery 9 4 17 16 32 51 39 53 42 52 40 22 19 15 1 2 4 1 2 1 1 0 3
Nucleolus 20 7 15 13 27 47 52 43 53 33 45 7 14 5 3 1 4 0 0 1 1 2 3
Peroxisomes 2 1 1 5 10 1 30 2 8 3 5 11 13 25 4 1 2 3 2 19 10 18 13
SpindlePole 0 2 2 0 4 1 7 4 3 2 2 1 0 1 0 0 0 0 1 1 2 1 2
Vac/Vac Membrane 55 19 11 1 24 19 18 15 22 7 10 29 27 49 21 8 13 13 11 2 5 7 19
Unique Cell Count 299 205 241 287 372 387 577 422 560 424 500 259 390 429 110 26 62 299 218 242 166 276 342
Labelled Cell Count 383 238 298 351 469 484 713 543 679 528 614 343 481 543 132 29 65 299 218 242 166 276 342


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.2 12.8 7.3 8.8 10.0 8.7 8.3 7.6 7.8 7.6 7.5 11.4 11.3 11.0 13.7 12.0 12.3 11.3 11.6 11.8
Std Deviation (1e-4) 1.9 2.5 1.8 2.2 2.8 2.2 2.3 2.4 2.2 2.0 2.2 2.3 2.1 2.0 3.4 3.2 3.6 2.8 2.6 2.7
Intensity Change (Log2) 0.26 0.45 0.26 0.19 0.06 0.1 0.05 0.04 0.65 0.63 0.6 0.91 0.72 0.75 0.63 0.67 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1.7 0 0 -0.6 0.3 -0.3 -0.1 -0.1 -1.0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.5 0 0 0 0 0 0 0 1.3 1.1 0 0 0 0
Cytoplasm 0.1 0.9 -0.6 -0.9 -0.5 -2.1 -1.2 -2.0 -0.2 -1.0 0.9 1.4 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.2 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 3.6 3.8 5.5 12.0 0 0
Mitochondria 0.2 -2.6 -1.5 -1.3 0.2 -0.4 0.5 1.6 -1.0 -1.5 -4.1 -15.8 -12.1 -13.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.7 0.7 2.4 -0.2 2.2 0.2 2.1 0.5 0.6 -1.1 -2.1 -2.4 0 0
Nucleolus -0.9 0.5 2.4 1.3 1.7 1.5 0.7 1.3 -1.9 -1.5 -3.7 -1.4 0 0
Peroxisomes 0 0 0 3.3 0 0 0 0 2.8 2.4 3.5 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.2 1.0 0.2 -1.0 -0.6 -0.4 -2.2 -2.0 2.7 1.2 3.0 4.4 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.6129 7.2013 7.0494 6.0699 6.5406 7.2731 6.976 7.2432 7.1523 6.4457 6.7144 6.8369 8.82 8.5336 8.6282 8.8433 6.5788 8.2285
Actin 0.0439 0.0037 0.0146 0.0833 0.0028 0.0006 0.0262 0.0126 0.032 0.0014 0.0017 0.0069 0.0145 0.0126 0.0084 0.0153 0.0016 0.0204
Bud 0.0006 0.0003 0.0001 0.0004 0.0003 0 0.0007 0.0008 0.0016 0 0.0001 0.0002 0.0004 0.0006 0.0027 0.0007 0.0073 0.0008
Bud Neck 0.0011 0.0001 0.0001 0.0162 0.0002 0 0.0007 0.0002 0.0003 0 0.0001 0.0002 0.0002 0.0002 0.0001 0.0003 0.0002 0.0002
Bud Periphery 0.0008 0.0004 0.0002 0.0005 0.0005 0.0001 0.0011 0.0023 0.0025 0.0001 0.0002 0.0005 0.0004 0.0006 0.0061 0.0029 0.0289 0.0021
Bud Site 0.0061 0.0056 0.0004 0.0042 0.0016 0 0.0042 0.0026 0.0162 0.0001 0.0001 0.0004 0.0013 0.0084 0.0009 0.0008 0.0017 0.0005
Cell Periphery 0.0004 0.0002 0.0001 0.0004 0.0001 0.0001 0.0005 0.0002 0.0006 0.0002 0.0001 0.0004 0.0001 0.0002 0.0002 0.0003 0.0003 0.0001
Cytoplasm 0.0035 0 0.0018 0.0023 0.0001 0 0.0018 0.0001 0.0001 0 0 0.0001 0.0024 0.0002 0.0002 0.0001 0.0007 0.0002
Cytoplasmic Foci 0.006 0.0018 0.0067 0.0368 0.0071 0.0002 0.0204 0.0042 0.0029 0.0015 0.0011 0.003 0.0097 0.0074 0.0043 0.001 0.0092 0.0048
Eisosomes 0.0003 0.0005 0.0005 0.0005 0.0001 0.0001 0.0004 0.0003 0.0005 0.0001 0.0002 0.0005 0.0002 0.0004 0.0002 0.0003 0.0001 0.0002
Endoplasmic Reticulum 0.0006 0 0.0005 0.0005 0.0001 0 0.0007 0.0001 0.0001 0 0.0001 0.0002 0.0003 0.0001 0.0004 0.0004 0.0008 0.0002
Endosome 0.013 0.0037 0.0194 0.0153 0.0173 0.0013 0.0265 0.0063 0.0061 0.0015 0.0037 0.0057 0.0103 0.0103 0.0079 0.0037 0.0624 0.0067
Golgi 0.0101 0.0162 0.0116 0.0181 0.0342 0.0023 0.0183 0.0215 0.0273 0.0044 0.0068 0.0175 0.0145 0.0326 0.0086 0.0048 0.037 0.0267
Lipid Particles 0.0075 0.003 0.0202 0.0229 0.0162 0.0042 0.041 0.0082 0.0025 0.0157 0.019 0.0122 0.0079 0.0133 0.013 0.0029 0.012 0.0213
Mitochondria 0.8676 0.9439 0.8684 0.7444 0.8863 0.969 0.7991 0.918 0.8837 0.9664 0.9601 0.9356 0.9079 0.894 0.9322 0.9586 0.7734 0.9
None 0.0091 0 0.0072 0.0026 0.0001 0 0.0024 0 0.0001 0 0.0001 0.0001 0.0042 0.0001 0.0005 0.0001 0.0004 0.0003
Nuclear Periphery 0.0004 0 0.0045 0.0002 0.0001 0 0.0014 0.0001 0.0001 0 0.0002 0.0041 0.0007 0.0009 0.001 0.0005 0.0006 0.0001
Nucleolus 0.0006 0.0001 0.0005 0.0003 0.0001 0.0001 0.0097 0.0002 0.0002 0.0001 0.0001 0.0001 0.0005 0.0002 0.0005 0.0001 0.0003 0.0001
Nucleus 0.0005 0 0.0075 0.0002 0.0001 0.0001 0.0031 0.0001 0.0001 0 0.0001 0.0001 0.0011 0.0001 0.0004 0.0002 0.0006 0.0001
Peroxisomes 0.0173 0.0177 0.0234 0.0477 0.0307 0.0061 0.0293 0.0183 0.0106 0.0073 0.0033 0.0083 0.0165 0.0119 0.0066 0.0058 0.0063 0.0128
Punctate Nuclear 0.0014 0 0.0025 0.0016 0.0001 0 0.0022 0 0 0 0.0001 0.0001 0.005 0.0001 0.0012 0.0002 0.0002 0.0007
Vacuole 0.004 0.0005 0.0035 0.0007 0.0008 0.0064 0.0052 0.0013 0.0081 0.0002 0.0006 0.0008 0.0007 0.0018 0.0017 0.0004 0.0189 0.0008
Vacuole Periphery 0.0052 0.0021 0.0062 0.0011 0.0013 0.0095 0.0051 0.0026 0.0043 0.0006 0.0023 0.0031 0.001 0.0041 0.0033 0.0007 0.037 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 52.5431 46.9703 22.6551 32.6897 45.984 55.1397 49.2726 60.9019 46.0123 51.5392
Translational Efficiency 1.0636 0.9781 1.6187 1.1367 1.0858 1.229 1.1418 1.164 1.412 1.4024

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1116 1488 198 101 1387 1475 2702 1365 2503 2963 2900 1466

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 957.38 1101.46 1438.57 1511.02 803.06 1106.06 1369.35 1221.26 871.87 1103.75 1374.08 1241.22
Standard Deviation 182.99 217.54 249.81 231.38 245.57 201.62 215.91 234.77 232.87 209.78 219.09 245.75
Intensity Change Log 2 0.202254 0.587472 0.658359 0.461850 0.769912 0.604791 0.326490 0.673566 0.634169

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001575 0.002904 0.007990 0.006781 0.001565 0.003530 0.000969 0.003664 0.001569 0.003215 0.001449 0.003879
Bud Neck 0.001835 0.001102 0.004123 0.006312 0.000551 0.000695 0.001115 0.004938 0.001123 0.000899 0.001320 0.005032
Bud Site 0.003896 0.003163 0.006553 0.007605 0.003537 0.002080 0.002080 0.016223 0.003697 0.002624 0.002386 0.015630
Cell Periphery 0.000600 0.000253 0.000247 0.000500 0.000405 0.000135 0.000092 0.001180 0.000492 0.000195 0.000102 0.001133
Cytoplasm 0.000522 0.000704 0.001102 0.000572 0.002180 0.000548 0.000677 0.002840 0.001440 0.000627 0.000706 0.002683
Cytoplasmic Foci 0.013850 0.015502 0.035299 0.027076 0.004346 0.009243 0.005869 0.011842 0.008584 0.012386 0.007879 0.012891
Eisosomes 0.000120 0.000146 0.000189 0.000605 0.000071 0.000093 0.000060 0.000173 0.000093 0.000120 0.000069 0.000203
Endoplasmic Reticulum 0.000278 0.000224 0.000482 0.000466 0.000147 0.000157 0.000059 0.001280 0.000205 0.000191 0.000088 0.001224
Endosome 0.014003 0.012282 0.026522 0.010770 0.004144 0.009259 0.004404 0.021235 0.008540 0.010777 0.005914 0.020514
Golgi 0.019304 0.027174 0.057095 0.053151 0.007870 0.024036 0.010457 0.036165 0.012968 0.025612 0.013641 0.037335
Lipid Particles 0.008694 0.005043 0.005924 0.002297 0.003860 0.004182 0.000984 0.006842 0.006015 0.004614 0.001321 0.006529
Mitochondria 0.902288* 0.905685* 0.770691* 0.807263* 0.948625* 0.924845* 0.950033* 0.821761* 0.927965* 0.915223* 0.937789* 0.820762*
Mitotic Spindle 0.001938 0.001752 0.012162 0.008981 0.000936 0.001481 0.002732 0.009148 0.001383 0.001617 0.003376 0.009137
None 0.000644 0.000528 0.001379 0.000842 0.000942 0.000367 0.000579 0.000995 0.000809 0.000447 0.000634 0.000984
Nuclear Periphery 0.000320 0.000097 0.001179 0.000470 0.000050 0.000080 0.000289 0.002136 0.000171 0.000088 0.000349 0.002021
Nuclear Periphery Foci 0.000517 0.000159 0.000891 0.000288 0.000094 0.000134 0.000166 0.001072 0.000282 0.000146 0.000215 0.001018
Nucleolus 0.000348 0.000220 0.000257 0.000125 0.000656 0.000228 0.000269 0.001095 0.000519 0.000224 0.000268 0.001028
Nucleus 0.000149 0.000308 0.000099 0.000062 0.001132 0.000155 0.000078 0.000582 0.000694 0.000232 0.000080 0.000546
Peroxisomes 0.018810 0.012748 0.024463 0.053927 0.016054 0.011586 0.004879 0.007845 0.017283 0.012170 0.006216 0.011020
Vacuole 0.005292 0.006511 0.018659 0.005034 0.001144 0.003810 0.005763 0.025384 0.002993 0.005166 0.006643 0.023982
Vacuole Periphery 0.005018 0.003494 0.024694 0.006875 0.001692 0.003356 0.008446 0.023601 0.003175 0.003426 0.009555 0.022449

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.43 -3.03 -2.63 -1.97 0.45 -2.12 1.62 -3.08 -0.13 -4.10 -3.08 0.32 -3.62 -0.88 -3.77
Bud Neck 1.45 -2.90 -1.39 -1.63 -0.67 -0.82 -3.36 -5.46 -5.21 -4.67 0.89 -0.85 -4.86 -5.27 -4.67
Bud Site 0.53 -1.47 -0.75 -0.90 -0.10 1.18 1.23 -5.36 -6.33 -6.47 1.17 1.38 -5.56 -6.26 -6.39
Cell Periphery 1.55 1.58 0.30 -0.94 -0.96 2.53 2.98 -3.40 -5.12 -5.35 2.56 3.43 -2.91 -4.89 -5.41
Cytoplasm -0.62 -1.36 -0.21 0.53 1.32 2.12 1.98 -0.12 -3.19 -2.96 1.88 1.70 -1.11 -3.06 -2.88
Cytoplasmic Foci -0.86 -3.51 -1.85 -1.61 0.90 -4.27 -1.74 -6.30 -1.97 -5.31 -3.42 0.68 -3.43 -0.40 -4.06
Eisosomes -1.40 -2.57 -2.46 -2.33 -2.10 -2.30 1.39 -4.76 -3.74 -5.47 -2.65 2.75 -4.48 -3.43 -5.65
Endoplasmic Reticulum 0.87 -1.91 -1.14 -1.46 0.25 -0.21 3.83 -3.76 -3.71 -4.06 0.39 4.38 -3.63 -3.67 -4.04
Endosome 0.85 -2.68 1.00 0.50 3.03 -4.57 -0.41 -9.90 -6.27 -9.97 -2.04 2.81 -6.92 -5.67 -9.03
Golgi -2.69 -4.44 -2.52 -1.93 0.25 -6.39 -1.81 -8.78 -3.20 -8.13 -6.65 -0.44 -7.71 -3.50 -7.54
Lipid Particles 2.02 1.26 3.71 2.19 2.07 -0.25 3.48 -2.23 -1.88 -5.38 1.33 5.58 -0.41 -1.60 -5.09
Mitochondria -0.37 5.93 3.14 3.27 -1.05 3.42 -0.39 13.88 11.21 15.37* 2.28 -1.99 12.53 11.21 14.25
Mitotic Spindle 0.21 -3.81 -2.02 -2.10 0.88 -1.10 -4.36 -5.41 -4.90 -4.17 -0.50 -3.97 -5.31 -5.18 -3.90
None 1.14 -4.42 -1.51 -2.92 3.14 1.76 1.20 0.10 -12.22 -7.81 1.91 1.06 -0.57 -10.46 -6.87
Nuclear Periphery 3.23 -2.44 -0.87 -2.36 1.87 -1.66 -5.58 -5.02 -4.95 -4.42 2.54 -3.29 -4.77 -4.99 -4.29
Nuclear Periphery Foci 3.09 -1.49 1.69 -1.60 2.52 -0.72 -1.94 -4.95 -4.79 -4.64 2.58 1.26 -3.88 -4.80 -4.40
Nucleolus 1.12 0.83 2.20 1.75 2.58 0.88 0.81 -0.64 -1.93 -1.87 1.06 0.92 -1.02 -1.95 -1.85
Nucleus -0.81 0.57 2.38 1.20 1.23 1.69 1.83 0.98 -2.30 -3.11 1.37 1.91 0.50 -1.61 -3.08
Peroxisomes 2.03 -0.84 -2.06 -2.44 -1.64 1.70 4.82 3.26 2.05 -2.20 2.59 6.05 2.69 0.60 -2.69
Vacuole -0.81 -3.48 0.14 0.74 3.36 -3.40 -7.69 -10.86 -9.31 -8.66 -2.59 -5.36 -9.97 -8.61 -8.12
Vacuole Periphery 1.96 -4.43 -0.85 -1.59 3.66 -3.15 -8.27 -12.42 -11.41 -8.05 -0.56 -7.67 -11.63 -11.55 -7.21
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrpl50

Mrpl50


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl50-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available