Standard name
Human Ortholog
Description Alpha-1,6-mannosyltransferase localized to the ER; responsible for addition of alpha-1,6 mannose to dolichol-linked Man7GlcNAc2; acts in the dolichol pathway for N-glycosylation; human homolog ALG12 complements yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.11 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.06 0 0 0 0 0.07 0.07 0.05 0.08 0.11 0.22 0.18 0.11 0 0 0 0 0 0
Cytoplasm 0.1 0.19 0.15 0.17 0.15 0.13 0.14 0.17 0.11 0.17 0.2 0.14 0.07 0.15 0.17 0.39 0.39 0.42 0 0.05 0 0 0 0
Endoplasmic Reticulum 0.81 0.72 0.5 0.39 0.34 0.29 0.36 0.25 0.37 0.26 0.25 0.27 0.8 0.79 0.75 0.09 0 0.06 0.87 0.8 0.87 0.84 0.77 0.81
Endosome 0 0 0.05 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0.29 0.51 0.26 0.47 0.46 0.45 0.64 0.59 0.52 0.57 0 0 0 0.17 0.21 0.14 0 0.06 0 0.06 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0 0.17 0.12 0.31 0.27 0.22 0.27 0.13 0.22 0.12 0.16 0.18 0.05 0 0.09 0.21 0.28 0 0 0 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 8 6 7 5 2 3 9 7 8 8 7 12 6 4 2 4 4 3 3 8 3 20 10
Bud 0 3 6 3 4 4 4 10 7 8 7 3 3 2 1 0 2 1 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 2 1
Bud Site 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 0 7 8 9 20 17 14 12 18 25 34 25 13 25 5 13 4 0 0 1 4 10 14
Cytoplasm 15 23 35 37 38 42 63 87 32 72 68 66 33 26 37 9 28 15 5 7 2 5 7 4
Endoplasmic Reticulum 125 86 117 83 89 92 166 126 104 113 86 128 365 137 168 2 0 2 162 116 247 300 378 427
Endosome 0 0 11 7 21 9 9 12 1 4 4 4 3 1 0 0 1 0 1 0 1 1 3 7
Golgi 1 0 2 1 2 0 0 0 0 0 0 0 1 0 0 1 1 0 0 1 0 2 2 6
Mitochondria 2 11 69 109 67 150 211 223 181 256 176 264 0 0 0 4 15 5 5 8 7 21 30 20
Nucleus 2 0 0 3 0 2 0 8 1 2 1 3 7 1 2 0 1 0 0 0 0 0 0 0
Nuclear Periphery 1 5 5 2 6 3 21 14 17 16 7 20 8 0 1 0 1 0 0 2 2 8 4 4
Nucleolus 0 0 1 0 0 1 0 4 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 1 1 0 1 1 1 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 17 5 39 25 80 85 103 132 36 94 40 73 80 9 1 2 15 10 4 5 2 8 23 26
Unique Cell Count 155 119 234 214 262 317 462 498 284 432 338 466 457 173 224 23 72 36 187 146 285 359 489 530
Labelled Cell Count 174 141 298 286 321 411 599 640 398 595 424 603 537 195 240 26 81 41 187 146 285 359 489 530


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 6.0 7.0 7.5 7.6 6.7 6.3 6.4 6.1 5.6 6.3 5.8 9.1 8.6 9.5 7.2 7.8 9.3 9.1 9.4 9.7
Std Deviation (1e-4) 1.0 0.8 1.4 1.4 1.5 1.6 1.2 1.4 1.3 1.2 2.6 1.3 1.1 1.2 1.4 1.9 1.6 3.0 1.6 1.8 1.5
Intensity Change (Log2) – – – 0.1 0.12 -0.06 -0.15 -0.13 -0.21 -0.32 -0.16 -0.28 0.38 0.28 0.44 0.03 0.16 0.41 0.37 0.41 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.4 -0.5 0 0 0 -0.1 0 -0.2 0 0 0.5 0 0 0 0
Bud 0 0 0 0 -0.5 -0.1 0 -0.4 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.4 0.3 1.8 0.5 -0.1 0.7 0.8 2.3 2.3 1.5 2.1 3.4 0 0 0
Cytoplasm 0.7 -0.1 -0.6 -0.5 0.9 -1.2 0.6 1.6 -0.3 -3.2 0 0.5 0 4.4 3.8
Endoplasmic Reticulum -2.4 -3.6 -5.0 -3.6 -6.6 -3.1 -6.2 -6.0 -5.9 8.1 6.0 5.5 -3.8 -7.6 -5.0
Endosome -0.8 1.5 -1.2 -2.1 -1.7 -3.3 -3.1 -2.6 -3.3 0 -2.4 -3.3 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.6 -1.0 4.2 4.1 3.9 7.8 7.3 5.4 6.8 -12.2 -7.8 -8.8 -1.2 -1.4 -2.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.1 0 1.6 0.5 2.2 1.1 0 1.4 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.5 3.6 2.8 1.7 2.9 -1.3 1.6 -1.6 -0.3 0.3 -3.5 -6.1 0 0.8 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0002 5.207 4.9892 4.5465 4.1023 4.779 4.889 6.0446 5.6256 5.2558 4.6596 5.4625 5.9466 5.9405 5.4432 5.1365 5.6085 5.2117
Actin 0.0067 0.0024 0.0022 0.0265 0.0005 0.0045 0.032 0.0002 0.0085 0.0003 0.0044 0.0009 0 0.0009 0.0088 0.0043 0.0006 0.0003
Bud 0.0001 0.0002 0.0001 0.0003 0.0001 0 0.0004 0 0.0001 0 0 0 0 0.0001 0 0.0006 0 0
Bud Neck 0.0006 0.0005 0.0002 0.0278 0.0098 0.0009 0.0002 0.0001 0.0003 0.0009 0.0046 0.0003 0 0.0003 0.0015 0.0011 0.0002 0.0008
Bud Periphery 0.0002 0.0004 0.0001 0.001 0.0001 0.0001 0.0006 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0064 0 0
Bud Site 0.0002 0.0024 0.0005 0.0004 0.0003 0.0001 0.0016 0.0004 0.0011 0 0.0001 0 0 0.0091 0.0006 0.0008 0 0
Cell Periphery 0.0011 0.0038 0.0008 0.0015 0.0006 0.0006 0.0017 0.0035 0.0013 0.0013 0.0009 0.0002 0.0007 0.002 0.0029 0.016 0.0005 0.0006
Cytoplasm 0.0562 0.0989 0.0477 0.0237 0.0957 0.0661 0.019 0.0568 0.0341 0.0028 0.0466 0.0343 0.0074 0.0596 0.0477 0.029 0.019 0.0446
Cytoplasmic Foci 0.0087 0.0065 0.0099 0.0025 0.0013 0.003 0.0033 0.0043 0.0038 0.0001 0.0009 0.002 0.0001 0.0022 0.0023 0.0213 0.0016 0.0022
Eisosomes 0.0002 0.0003 0.0002 0.0003 0 0 0.0012 0 0.0001 0 0.0001 0 0 0.0001 0.0001 0.0005 0 0
Endoplasmic Reticulum 0.8285 0.7797 0.8774 0.8525 0.8291 0.8817 0.8472 0.8586 0.9211 0.9797 0.9273 0.9257 0.9714 0.8964 0.9106 0.8727 0.9576 0.9287
Endosome 0.0256 0.0316 0.0215 0.0158 0.0072 0.0112 0.0188 0.0222 0.0062 0.0021 0.0057 0.0166 0.0008 0.0066 0.009 0.0063 0.0042 0.0071
Golgi 0.0037 0.006 0.0044 0.0054 0.0004 0.001 0.0083 0.0013 0.0029 0.0003 0.0013 0.0018 0.0001 0.0013 0.0026 0.0042 0.0008 0.001
Lipid Particles 0.0058 0.0198 0.0126 0.0049 0.0012 0.003 0.0109 0.0176 0.0044 0.0008 0.0005 0.0021 0.0026 0.0034 0.0024 0.0215 0.0089 0.0028
Mitochondria 0.0014 0.0055 0.0006 0.0077 0.0002 0.0019 0.0013 0.0037 0.0007 0.0002 0.0003 0.0013 0 0.0003 0.0005 0.0009 0.0001 0.0002
None 0.0063 0.0094 0.0066 0.0016 0.0399 0.0027 0.0265 0.0117 0.0048 0.0001 0.0007 0.0015 0.0001 0.0027 0.0024 0.0008 0.0002 0.002
Nuclear Periphery 0.0312 0.0169 0.0097 0.0167 0.0071 0.0134 0.0177 0.0108 0.0043 0.008 0.0048 0.0089 0.0151 0.0086 0.0055 0.0029 0.0041 0.0067
Nucleolus 0.0002 0.0001 0 0.0003 0 0 0.0001 0 0 0 0 0 0 0 0 0.0002 0 0
Nucleus 0.0138 0.0031 0.0009 0.0036 0.0029 0.0017 0.0009 0.0024 0.0004 0.0005 0.0005 0.0006 0.0002 0.0006 0.0003 0.0002 0.0003 0.0005
Peroxisomes 0.0005 0.0001 0.0002 0.0006 0 0.0035 0.0005 0 0.0028 0 0 0 0 0 0.0001 0.0065 0 0
Punctate Nuclear 0.0045 0.0005 0.0004 0.0022 0.0003 0.0008 0.0004 0.0004 0.0009 0 0.0001 0.0003 0 0.0001 0.0001 0.002 0.0001 0.0002
Vacuole 0.0035 0.0107 0.0035 0.0036 0.0027 0.0031 0.0058 0.0053 0.0019 0.0023 0.0008 0.0023 0.0013 0.005 0.002 0.0014 0.0015 0.002
Vacuole Periphery 0.0012 0.0011 0.0006 0.0011 0.0003 0.0006 0.0014 0.0006 0.0002 0.0005 0.0002 0.0008 0.0002 0.0005 0.0003 0.0003 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 46.9746 35.7383 34.4751 44.6868 38.4434 32.631 34.6293 42.4769 37.3433 50.94
Translational Efficiency 0.831 0.7234 0.8698 0.6533 0.6877 0.9661 0.8647 0.6158 0.8376 0.6293

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alpha-1,6-mannosyltransferase localized to the ER; responsible for addition of alpha-1,6 mannose to dolichol-linked Man7GlcNAc2; acts in the dolichol pathway for N-glycosylation; human homolog ALG12 complements yeast null mutant
Localization
Cell Percentages ER (95%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Alg12

Alg12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alg12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available