Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.08 0.21 0.24 0.15 0.37 0.42 0.4 0.47 0.47 0.53 0.51 0.48 0.78 0.92 0.11 0.16 0.09 0 0 0 0 0 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.29 0.05 0.05 0 0.06 0.09 0.16 0.05 0.13 0.1 0.17 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.25 0.22 0.32 0.36 0.26 0.23 0.15 0.19 0.16 0.1 0.1 0.09 0.06 0 0 0.42 0.38 0.43 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.58 0.75 0.55 0.55 0.7 0.6 0.51 0.52 0.52 0.54 0.47 0.54 0.45 0.2 0.09 0.53 0.55 0.57 0.81 0.81 0.89 0.81 0.82 0.79
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 1 1 1 1 0 0 0 0 0 0 1 0 0 1 2
Bud 2 0 0 0 0 1 5 5 4 7 4 4 1 1 0 0 0 0 6 8 2 11 23 16
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 1 0 0 0 0 0
Bud Site 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 24 14 38 36 42 105 152 149 184 151 138 141 207 606 714 53 47 35 1 1 0 11 21 32
Endoplasmic Reticulum 0 0 1 0 1 0 1 0 1 0 1 0 4 4 1 18 6 9 0 0 0 0 3 3
Endosome 0 9 6 5 7 3 1 7 2 4 0 1 43 31 9 18 9 7 7 6 5 10 22 19
Golgi 0 1 3 0 2 0 1 0 0 0 0 0 1 1 1 7 3 4 0 1 1 2 2 1
Mitochondria 52 8 9 6 16 26 56 18 50 33 44 26 6 1 1 7 10 8 4 2 1 3 10 12
Nucleus 46 37 57 54 75 65 53 70 61 33 26 26 27 18 9 200 116 162 8 7 4 6 16 5
Nuclear Periphery 4 0 2 1 1 1 4 0 1 3 3 0 1 0 0 4 0 2 1 1 2 2 4 4
Nucleolus 3 2 1 0 0 0 2 0 3 0 0 0 4 0 0 5 7 0 2 2 0 1 1 2
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 2 3 1 2 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 3 0 2 0 2 0 0 0 1 9 9 3 4 7 2 1 0 1 9 13 14
Vac/Vac Membrane 105 128 97 84 199 170 183 193 202 173 121 149 193 159 72 251 167 214 161 159 175 266 584 453
Unique Cell Count 182 170 177 152 286 285 359 369 390 322 260 278 433 776 777 474 303 374 201 196 197 331 714 571
Labelled Cell Count 238 200 216 189 343 373 458 445 510 407 338 349 501 834 812 569 373 443 201 196 197 331 714 571


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 9.2 7.3 6.9 7.9 6.5 6.2 6.9 5.8 6.1 5.5 5.9 5.9 5.4 5.2 11.1 11.6 10.8 6.4 6.3 6.3
Std Deviation (1e-4) 2.8 4.5 2.7 2.6 2.6 2.9 2.8 2.7 2.7 2.6 2.4 2.3 1.8 1.5 1.5 5.2 5.5 5.1 2.4 2.1 1.9
Intensity Change (Log2) – – – -0.08 0.11 -0.16 -0.24 -0.08 -0.33 -0.25 -0.41 -0.31 -0.3 -0.44 -0.48 0.61 0.67 0.57 -0.19 -0.21 -0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 -1.9 3.5 4.8 4.4 5.8 5.6 6.6 6.2 6.0 14.5 20.7 -3.4 -1.6 -3.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.2 0 0
Endosome -0.1 0 0 0 0 0 0 0 0 2.7 0.4 0 0.2 -0.3 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.5 0.2 1.6 3.5 -0.1 2.8 2.0 3.7 1.7 0 0 0 0 -1.0 -1.9
Nucleus 0.6 -1.4 -2.2 -4.7 -3.4 -4.5 -6.1 -5.8 -6.2 -8.4 -13.3 -14.7 2.3 1.3 2.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.1 3.2 1.0 -0.8 -0.5 -0.7 -0.2 -1.7 -0.3 -2.3 -9.3 -14.3 -0.4 0.1 0.5