Standard name
Human Ortholog
Description 1,3-beta-glucanosyltransferase; has similarity to Gas1p; localizes to the cell wall
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.33 0.31 0.38 0.67 0.47 0.67 0.53 0.5 0.78 0.67 0.82 0.83 0.71 0.8 0.87 0.29 0.24 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0.13 0.05 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.05 0 0 0 0 0.05 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0.22 0.16 0.09 0 0.08 0.14 0.13 0.08 0.1 0.05 0.1 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.09 0.13 0.06 0.08 0 0 0 0 0 0.22 0.2 0.27 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.68 0.64 0.56 0.43 0.62 0.42 0.48 0.41 0.3 0.41 0.17 0.24 0.33 0.31 0.18 0.64 0.67 0.63 0.89 0.86 0.85 0.72 0.86 0.84
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud 2 0 2 0 0 0 0 4 3 0 4 6 2 0 0 0 1 1 0 1 1 0 0 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 85 21 44 101 86 137 115 130 212 228 188 244 205 312 371 79 53 35 4 3 5 5 4 5
Endoplasmic Reticulum 2 0 1 3 2 7 2 3 4 3 1 1 5 3 1 13 15 7 6 0 2 26 14 8
Endosome 0 0 3 1 4 2 0 0 0 0 0 0 17 2 3 11 12 6 6 2 3 10 9 15
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 3 0 2 0 0 1 0 3
Mitochondria 38 15 19 13 7 16 31 34 21 34 12 30 2 1 3 17 9 3 5 2 3 4 6 1
Nucleus 9 3 4 3 3 8 19 33 16 28 6 10 1 2 3 61 45 48 0 0 1 0 0 1
Nuclear Periphery 2 0 0 2 0 2 1 0 1 1 1 0 0 0 2 0 1 0 2 0 0 2 2 3
Nucleolus 0 1 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 1 1 0 0 0 0 0 0 0 2 0 0 1 1 0 0 0 0 0 0 1
Vac/Vac Membrane 173 43 65 65 115 86 104 107 83 138 40 71 96 123 75 177 148 113 262 85 115 147 255 261
Unique Cell Count 254 67 117 151 184 205 218 261 273 340 229 295 290 391 425 275 220 179 296 99 136 204 298 312
Labelled Cell Count 312 83 138 189 218 258 273 311 340 433 252 364 330 443 460 360 289 213 296 99 136 204 298 312


Endoplasmic Reticulum, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.3 4.9 5.1 6.6 6.4 5.2 5.5 5.9 5.4 5.4 5.2 5.7 5.0 4.7 6.9 8.3 9.0 6.9 6.4 7.0
Std Deviation (1e-4) 0.8 1.0 1.2 1.0 1.4 1.4 1.6 1.5 1.6 1.4 1.6 1.5 1.1 1.1 1.2 1.8 2.4 2.4 1.5 1.4 1.7
Intensity Change (Log2) – – – 0.07 0.44 0.39 0.1 0.18 0.27 0.15 0.14 0.09 0.21 0.04 -0.06 0.51 0.77 0.88 0.5 0.39 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4.8 1.6 5.1 2.6 2.2 7.6 5.6 8.3 9.0 6.2 8.7 11.2 -1.7 -2.6 -3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2.5 0
Endosome 0 0 0 0 0 0 0 0 0 1.4 0 0 0 1.2 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.9 -3.7 -2.3 -0.5 -0.8 -2.5 -1.8 -3.4 -1.7 -6.4 0 0 -3.2 -3.8 -4.7
Nucleus 0 0 0 1.8 2.8 1.0 1.8 0 0 0 0 0 4.6 4.2 5.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.0 1.2 -2.4 -1.4 -2.6 -4.7 -2.8 -7.3 -6.1 -4.2 -4.7 -8.3 1.6 2.1 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3702 6.3557 5.6232 5.4584 4.8547 5.9711 2.2848 3.9903 3.2606 2.6646 2.8174 3.6623 4.0977 6.0985 4.8542 4.7815 4.6232 4.9721
Actin 0.0136 0.0057 0.001 0.0107 0.0003 0.0057 0.0058 0.0001 0.0089 0.0005 0.0022 0.0009 0.0353 0.0002 0.0224 0.0435 0.0002 0.0002
Bud 0.0014 0.0003 0.0005 0.0007 0.0003 0.0005 0.0003 0.0004 0.0007 0.0003 0.0008 0.0004 0.0006 0.0004 0.0007 0.0006 0.0004 0.0005
Bud Neck 0.0033 0.0011 0.0015 0.0045 0.0012 0.0025 0.0009 0.0012 0.0045 0.0021 0.0016 0.0028 0.0048 0.0008 0.0021 0.0014 0.0015 0.0025
Bud Periphery 0.0049 0.001 0.0014 0.0032 0.0011 0.002 0.0009 0.0014 0.0022 0.0012 0.0028 0.0019 0.0012 0.0011 0.002 0.0014 0.0013 0.0022
Bud Site 0.0043 0.0017 0.0025 0.0024 0.0011 0.001 0.0022 0.002 0.0067 0.0016 0.0032 0.0012 0.0039 0.0032 0.0034 0.0029 0.0016 0.0011
Cell Periphery 0.0061 0.0041 0.0049 0.0068 0.0023 0.0059 0.0045 0.0115 0.0068 0.013 0.006 0.0062 0.0026 0.0048 0.0047 0.0032 0.0017 0.006
Cytoplasm 0.0154 0.0189 0.0159 0.0034 0.0035 0.0046 0.0247 0.0331 0.0393 0.0051 0.0191 0.0218 0.0217 0.0518 0.0303 0.0074 0.005 0.0123
Cytoplasmic Foci 0.0109 0.0095 0.009 0.0071 0.0047 0.0032 0.0263 0.0039 0.0207 0.034 0.0258 0.0088 0.0226 0.0086 0.0181 0.0062 0.0037 0.0082
Eisosomes 0.0001 0 0 0.0043 0 0 0.0001 0 0.0004 0 0.0001 0.0001 0.0004 0 0.0002 0.0002 0 0
Endoplasmic Reticulum 0.0236 0.0194 0.0267 0.0081 0.0177 0.0177 0.0347 0.0606 0.0357 0.0161 0.0175 0.0264 0.0313 0.0407 0.0852 0.0387 0.024 0.0417
Endosome 0.186 0.0758 0.183 0.1735 0.165 0.1168 0.2901 0.1247 0.2449 0.1593 0.166 0.1431 0.2104 0.1438 0.1795 0.1874 0.1778 0.1359
Golgi 0.0085 0.0032 0.0083 0.0197 0.0043 0.0034 0.0121 0.0028 0.0206 0.0063 0.0059 0.0068 0.0169 0.0032 0.0153 0.0115 0.0116 0.0068
Lipid Particles 0.0209 0.0031 0.0136 0.0112 0.0151 0.0078 0.0445 0.0107 0.0268 0.0193 0.0448 0.0141 0.0239 0.0071 0.0155 0.0099 0.0039 0.0129
Mitochondria 0.0204 0.0037 0.0164 0.0271 0.0125 0.003 0.0072 0.0021 0.0188 0.0085 0.0295 0.0073 0.007 0.0043 0.0079 0.0135 0.0219 0.0124
None 0.0025 0.0003 0.0009 0.001 0.0003 0.0003 0.0167 0.0011 0.043 0.002 0.0052 0.0284 0.0156 0.0014 0.0047 0.0013 0.0002 0.0048
Nuclear Periphery 0.023 0.0128 0.0181 0.0064 0.0143 0.0146 0.0258 0.0258 0.0184 0.0175 0.0044 0.0142 0.0228 0.0241 0.0237 0.018 0.0129 0.012
Nucleolus 0.0059 0.0029 0.0023 0.001 0.0121 0.002 0.005 0.0013 0.0024 0.0222 0.0023 0.0037 0.0026 0.001 0.0009 0.0014 0.0013 0.0017
Nucleus 0.0128 0.0066 0.0104 0.003 0.0093 0.0061 0.0076 0.004 0.0047 0.0231 0.005 0.0116 0.0106 0.0036 0.0045 0.0061 0.0028 0.006
Peroxisomes 0.004 0.0015 0.0042 0.0013 0.0004 0.0005 0.0101 0.0001 0.0079 0.015 0.0073 0.0024 0.0108 0.0001 0.004 0.0013 0.0002 0.0004
Punctate Nuclear 0.003 0.0002 0.0008 0.0004 0.0003 0.0004 0.0027 0.0001 0.0013 0.0019 0.0007 0.0009 0.0068 0.0003 0.0051 0.0018 0.0001 0.0002
Vacuole 0.5282 0.7162 0.5841 0.6062 0.6351 0.7012 0.4196 0.6557 0.4284 0.5715 0.5918 0.6026 0.4858 0.6435 0.5138 0.5781 0.6317 0.6501
Vacuole Periphery 0.1013 0.1118 0.0943 0.0979 0.099 0.1006 0.0582 0.0573 0.0569 0.0795 0.0576 0.0945 0.0625 0.056 0.0561 0.0644 0.0961 0.0822

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 106.1695 171.5142 161.4782 158.1329 110.6143 87.9203 205.1197 191.9914 153.3455 112.4062
Translational Efficiency 1.3084 1.4175 1.4553 1.2232 1.0939 1.7574 1.3203 1.2719 1.3315 1.2154

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description 1,3-beta-glucanosyltransferase; has similarity to Gas1p; localizes to the cell wall
Localization
Cell Percentages vacuole (60%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Gas5

Gas5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gas5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available