Standard name
Human Ortholog
Description Putative endonuclease; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0.08 0.14 0 0.05 0 0 0.06 0.05 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.16 0.26 0.29 0.36 0.31 0.19 0.1 0.11 0.08 0.09 0.07 0.06 0.34 0.44 0.52 0.47 0.41 0.39 0 0.05 0.06 0 0.06 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0.06 0 0.08 0 0.14 0.14 0.07 0 0 0 0 0 0.19 0.21 0.23 0.18 0.16 0.25 0.13 0.07 0.1 0.13 0.09 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.79 0.75 0.31 0.39 0.34 0.57 0.74 0.78 0.87 0.85 0.94 0.87 0.27 0.09 0.06 0.05 0.1 0.16 0.69 0.65 0.57 0.55 0.56 0.57
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.1 0 0 0 0 0 0 0 0 0
SpindlePole 0.07 0 0.27 0.2 0.2 0.12 0.13 0.13 0.06 0.08 0 0.07 0.12 0.16 0.13 0.16 0.19 0.18 0 0 0.12 0.08 0.09 0.06
Vac/Vac Membrane 0.08 0 0 0 0.08 0.11 0.08 0.06 0.07 0.06 0 0.1 0.14 0.09 0.07 0.13 0.12 0.13 0 0.1 0 0 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 3 8 6 0 0 1 2 1 0 1 8 5 1 7 4 5 3 2 1 4 10 20
Bud 9 9 7 5 14 6 19 28 23 22 18 27 13 8 5 6 6 8 0 1 1 4 6 12
Bud Neck 1 1 1 4 6 2 3 2 2 2 0 3 2 3 1 1 1 0 1 1 2 2 4 9
Bud Site 0 1 0 1 1 5 11 6 9 7 5 0 0 0 0 1 4 1 – – – – – –
Cell Periphery 1 0 0 9 1 2 4 0 3 3 1 5 2 6 3 13 9 4 0 0 0 0 0 1
Cytoplasm 33 30 15 64 87 55 47 50 37 42 23 26 102 158 209 137 63 70 2 6 8 10 22 26
Endoplasmic Reticulum 1 3 1 3 0 3 2 0 1 5 2 1 2 10 3 18 8 6 1 0 0 2 2 6
Endosome 12 1 4 3 39 41 31 18 6 6 1 7 58 74 93 53 24 44 28 8 13 30 34 55
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 3 3 0 3 5 2 3 1 0 3 7 6
Mitochondria 158 85 16 69 95 166 333 353 393 399 295 383 80 32 26 14 15 28 153 78 78 131 219 308
Nucleus 1 0 2 1 3 3 17 19 10 11 8 11 1 4 1 7 3 1 0 0 0 1 2 1
Nuclear Periphery 0 0 0 0 1 1 3 1 0 2 1 0 0 0 0 0 0 1 0 0 0 0 2 3
Nucleolus 1 2 0 0 0 1 3 2 1 2 4 2 1 0 1 0 1 1 0 0 1 2 1 4
Peroxisomes 15 1 0 4 5 2 12 4 5 2 1 5 20 25 42 3 2 8 9 2 3 4 11 14
SpindlePole 15 5 14 35 55 36 57 57 26 36 11 30 35 57 51 47 29 32 7 4 16 19 34 32
Vac/Vac Membrane 16 5 0 7 23 31 38 27 31 28 11 43 42 34 30 38 19 23 3 12 2 9 24 27
Unique Cell Count 201 114 51 176 282 293 451 451 450 468 314 442 301 360 404 292 152 178 222 122 136 240 394 544
Labelled Cell Count 264 148 63 213 336 354 580 568 549 568 381 544 369 419 466 348 193 234 222 122 136 240 394 544


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.9 4.8 4.9 5.2 4.5 4.1 4.6 4.0 4.1 4.0 4.2 5.6 5.8 5.6 6.2 6.2 6.4 5.2 5.3 5.7
Std Deviation (1e-4) 0.6 1.3 0.7 1.2 1.3 1.4 1.1 1.3 1.1 1.0 2.1 1.0 1.2 1.5 1.6 1.3 1.6 1.8 1.6 1.7 1.6
Intensity Change (Log2) – – – 0.04 0.1 -0.1 -0.23 -0.06 -0.26 -0.22 -0.28 -0.19 0.22 0.26 0.23 0.37 0.37 0.41 0.1 0.15 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 2.7 – 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7228 2.0541 1.9777 1.75 1.5107 1.8904 0.4934 1.0909 0.8361 0.7229 0.419 0.9441 0.4395 0.7317 0.5151 0.8072 0.9875 0.1897
Actin 0.0114 0.0063 0.0144 0.0245 0.0159 0.004 0.0276 0.0103 0.0137 0.0003 0.0069 0.0049 0.0594 0.0199 0.0609 0.0204 0.0007 0.0527
Bud 0.0006 0.0021 0.003 0.001 0.0019 0.0007 0.0014 0.0014 0.0015 0.0012 0.0031 0.0006 0.0011 0.0048 0.007 0.0017 0.0019 0.0014
Bud Neck 0.001 0.0025 0.0025 0.0003 0.0008 0.0013 0.0045 0.0012 0.0035 0.0005 0.001 0.0031 0.0021 0.0019 0.0054 0.0007 0.0019 0.015
Bud Periphery 0.0017 0.0032 0.0075 0.0022 0.0066 0.0018 0.004 0.002 0.0034 0.0024 0.0065 0.0015 0.0014 0.0066 0.0172 0.0027 0.0016 0.0032
Bud Site 0.0033 0.0211 0.009 0.0006 0.0028 0.0013 0.0081 0.0111 0.0163 0.0008 0.0033 0.0007 0.0096 0.0518 0.0291 0.0019 0.0166 0.0019
Cell Periphery 0.0014 0.0007 0.0015 0.0002 0.0014 0.0005 0.0019 0.0005 0.0041 0.0002 0.0005 0.0003 0.001 0.0017 0.0047 0.0004 0.0005 0.0008
Cytoplasm 0.0093 0.0116 0.0128 0.0039 0.0072 0.0049 0.0105 0.0182 0.0189 0.0057 0.0067 0.0099 0.0153 0.0245 0.0371 0.027 0.2015 0.0106
Cytoplasmic Foci 0.0421 0.0507 0.0341 0.0236 0.0303 0.0299 0.0595 0.0692 0.0721 0.0339 0.0528 0.0541 0.0729 0.0919 0.0858 0.0712 0.0988 0.0842
Eisosomes 0.001 0.0003 0.0009 0.0003 0.0016 0.0002 0.001 0.0002 0.001 0.0001 0.0002 0.0003 0.0009 0.0008 0.0014 0.0004 0 0.0004
Endoplasmic Reticulum 0.0031 0.0019 0.0013 0.0003 0.0017 0.0011 0.0065 0.0021 0.0015 0.0005 0.0022 0.0024 0.0058 0.0031 0.0045 0.0027 0.0022 0.0011
Endosome 0.1123 0.118 0.0856 0.035 0.0748 0.0548 0.1289 0.1068 0.0811 0.0971 0.1546 0.0895 0.2202 0.1384 0.1085 0.1509 0.1137 0.09
Golgi 0.0751 0.0898 0.0618 0.0226 0.0465 0.0442 0.0593 0.0794 0.0496 0.022 0.0726 0.0485 0.1242 0.0983 0.097 0.075 0.0171 0.1184
Lipid Particles 0.0356 0.0203 0.0184 0.0147 0.0333 0.0158 0.0559 0.0156 0.0156 0.0024 0.0147 0.0179 0.0696 0.0166 0.0112 0.0099 0.2373 0.0197
Mitochondria 0.6036 0.6044 0.6736 0.6965 0.6823 0.7743 0.5176 0.5876 0.627 0.7926 0.6174 0.666 0.3009 0.4397 0.4228 0.5848 0.2563 0.5361
None 0.0159 0.0106 0.0237 0.0877 0.0293 0.0087 0.0185 0.004 0.011 0.0009 0.0019 0.0249 0.0236 0.0329 0.0267 0.0157 0.0032 0.0019
Nuclear Periphery 0.0081 0.0007 0.0007 0.0012 0.006 0.0024 0.0049 0.0035 0.0017 0.0004 0.0008 0.0042 0.0018 0.0007 0.0008 0.0013 0.0008 0.0003
Nucleolus 0.0018 0.0015 0.0007 0.0008 0.0018 0.0008 0.0022 0.0006 0.0017 0.0003 0.0004 0.0006 0.0012 0.0005 0.0007 0.0003 0.0028 0.0002
Nucleus 0.0035 0.0023 0.0008 0.0009 0.0042 0.0029 0.0016 0.001 0.0023 0.0004 0.0005 0.001 0.0011 0.0007 0.0007 0.0008 0.0018 0.0004
Peroxisomes 0.0537 0.0328 0.0329 0.0722 0.0363 0.0428 0.0636 0.0602 0.0604 0.0152 0.0388 0.0589 0.0655 0.0502 0.0678 0.0177 0.0167 0.0529
Punctate Nuclear 0.0015 0.0007 0.0006 0.0082 0.0052 0.0015 0.0034 0.0004 0.0024 0.0002 0.0003 0.0015 0.0014 0.0004 0.0006 0.0006 0.0026 0.0005
Vacuole 0.0087 0.0128 0.0097 0.0019 0.0059 0.0029 0.0135 0.0193 0.0066 0.0121 0.0085 0.0046 0.0159 0.0107 0.0069 0.0095 0.0195 0.0041
Vacuole Periphery 0.0052 0.0057 0.0046 0.0013 0.0045 0.0032 0.0056 0.0057 0.0044 0.0109 0.0064 0.0046 0.0049 0.004 0.0032 0.0044 0.0024 0.0041

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.6514 11.7426 10.054 13.4354 7.8605 7.0692 15.0695 13.5612 14.6403 14.6656
Translational Efficiency 0.6281 0.6217 0.7509 0.4736 0.9462 1.6161 0.7606 0.7199 0.5787 0.6501

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
36 630 1510 1281 1411 1456 568 940 1447 2086 2078 2221

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 943.27 659.58 892.25 844.94 707.75 740.63 957.72 923.59 713.61 716.15 910.15 878.23
Standard Deviation 221.13 108.70 241.14 110.87 94.39 107.89 120.17 128.82 106.07 114.36 216.92 125.00
Intensity Change Log 2 — -0.516123 -0.080223 -0.158822 — 0.065513 0.436364 0.384013 — -0.237714 0.164144 0.099193

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.004390 0.003856 0.009163 0.023006 0.001069 0.007832 0.009658 0.037841 0.001152 0.006631 0.009299 0.029285
Bud Neck 0.001861 0.026524 0.020340 0.018297 0.021237 0.012351 0.023955 0.016834 0.020755 0.016631 0.021328 0.017678
Bud Site 0.012994 0.048172 0.084820 0.137332* 0.029644 0.027053 0.060289 0.146410* 0.029230 0.033431 0.078115 0.141174*
Cell Periphery 0.000526 0.000603 0.000187 0.000415 0.000473 0.000442 0.000256 0.000456 0.000474 0.000491 0.000206 0.000432
Cytoplasm 0.004666 0.021697 0.061919 0.068110 0.034485 0.014599 0.053812 0.086996 0.033744 0.016743 0.059703 0.076103
Cytoplasmic Foci 0.060975 0.105915 0.134798 0.135789 0.163439 0.119106 0.166845 0.155830 0.160890 0.115122 0.143557 0.144271
Eisosomes 0.000123 0.000159 0.000118 0.000183 0.000168 0.000252 0.000157 0.000218 0.000167 0.000224 0.000129 0.000198
Endoplasmic Reticulum 0.010104 0.000688 0.006179 0.003017 0.002748 0.000553 0.005719 0.002501 0.002931 0.000594 0.006053 0.002799
Endosome 0.051780 0.142634 0.073637 0.071988 0.100099 0.099217 0.126275 0.058738 0.098897 0.112330 0.088025 0.066380
Golgi 0.081898 0.127904* 0.064159 0.069707 0.117713* 0.210878* 0.039734 0.065516 0.116822* 0.185818* 0.057482 0.067933
Lipid Particles 0.003467 0.007102 0.003718 0.002706 0.009645 0.004857 0.019756 0.002265 0.009492 0.005535 0.008102 0.002519
Mitochondria 0.591782* 0.342728* 0.158029* 0.121240* 0.395056* 0.385455* 0.057686 0.064579 0.399950* 0.372551* 0.130601* 0.097259
Mitotic Spindle 0.016334 0.023658 0.163972* 0.091378 0.011278 0.017460 0.116248* 0.081705 0.011403 0.019332 0.150927* 0.087284
None 0.000390 0.003939 0.000490 0.000242 0.000532 0.000374 0.001134 0.000185 0.000529 0.001451 0.000666 0.000218
Nuclear Periphery 0.001942 0.000125 0.001203 0.000358 0.000424 0.000047 0.003131 0.000231 0.000462 0.000071 0.001730 0.000305
Nuclear Periphery Foci 0.020382 0.000576 0.001772 0.001134 0.000447 0.000161 0.004928 0.000839 0.000943 0.000287 0.002635 0.001009
Nucleolus 0.017414 0.005977 0.001568 0.000654 0.001390 0.000402 0.011717 0.000287 0.001789 0.002085 0.004342 0.000499
Nucleus 0.043430 0.003667 0.022468 0.011013 0.007886 0.001293 0.054106 0.008286 0.008771 0.002010 0.031116 0.009859
Peroxisomes 0.008232 0.100312* 0.065977 0.155581* 0.042078 0.073000 0.110046* 0.170918* 0.041236 0.081248 0.078023 0.162072*
Vacuole 0.044479 0.020014 0.109865 0.077846 0.036313 0.015176 0.121735 0.089937 0.036516 0.016637 0.113110 0.082964
Vacuole Periphery 0.022832 0.013751 0.015619 0.010004 0.023872 0.009491 0.012814 0.009427 0.023846 0.010778 0.014852 0.009760

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.24 -2.07 -6.15 -8.54 -6.02 -6.78 -5.05 -9.25 -7.12 -6.22 -7.29 -10.05 -13.73 -10.11 -8.74
Bud Neck -7.25 -14.69 -13.44 1.94 1.29 3.79 -0.88 1.74 -2.74 2.61 1.77 -0.75 1.02 -1.24 2.72
Bud Site -5.99 -14.26 -19.33 -12.55 -8.11 0.82 -5.85 -17.00 -17.46 -10.20 -1.45 -13.86 -24.16 -23.49 -12.20
Cell Periphery -0.52 2.47 0.96 2.88 -4.25 0.33 3.45 0.53 0.21 -1.95 -0.23 4.88 1.05 1.33 -4.41
Cytoplasm -5.41 -16.98 -17.35 -11.50 -1.62 7.81 -4.52 -11.49 -15.95 -5.48 6.85 -8.85 -13.66 -20.72 -4.87
Cytoplasmic Foci -2.47 -3.81 -3.89 -4.02 -0.29 7.95 -0.12 2.25 -4.82 1.98 8.72 3.88 4.07 -5.70 0.20
Eisosomes -1.23 0.14 -1.97 -1.61 -7.62 -3.18 0.58 -2.98 1.53 -3.38 -2.95 4.50 -2.64 1.67 -8.43
Endoplasmic Reticulum 2.16 0.83 1.61 -5.91 5.01 5.24 -3.91 0.28 -6.37 4.26 5.56 -5.79 0.01 -8.45 6.72
Endosome -5.49 -1.23 -1.08 9.66 0.57 0.17 -2.38 9.51 9.67 8.87 -2.63 3.35 8.52 12.68 6.22
Golgi -1.86 0.91 0.75 8.40 -0.90 -12.79 14.70 10.48 22.22 -4.37 -10.87 12.99 11.33 23.82 -2.38
Lipid Particles -1.70 -0.30 0.32 2.66 2.04 4.10 -4.04 7.17 3.12 7.43 3.49 1.04 7.00 3.89 7.55
Mitochondria 3.29 5.98 6.48 15.62 4.50 0.70 32.17* 33.49* 32.87* -0.74 2.31 27.68 31.93 33.84 5.49
Mitotic Spindle -0.48 -10.84 -5.65 -11.34 8.94 -2.96 -11.60 -11.38 -10.25 3.07 -4.05 -25.85 -18.39 -16.23 9.70
None -2.25 -0.98 1.28 2.34 4.20 0.78 -2.61 5.37 1.15 4.19 -1.89 -1.56 5.18 2.52 5.95
Nuclear Periphery 2.09 0.73 1.73 -4.34 5.72 3.10 -3.79 0.73 -4.41 3.98 3.25 -5.56 0.52 -6.63 6.50
Nuclear Periphery Foci 2.09 1.94 2.02 -2.18 1.77 3.64 -4.60 -1.54 -2.50 3.99 2.51 -4.38 -0.54 -3.90 4.23
Nucleolus 0.92 1.31 1.39 2.82 5.06 3.90 -4.70 4.46 0.54 5.26 -0.40 -3.68 3.19 2.62 6.33
Nucleus 2.00 0.85 1.45 -6.00 4.40 5.42 -8.12 -2.24 -7.18 7.36 5.20 -10.14 -2.95 -9.80 8.41
Peroxisomes -11.50 -11.78 -20.11 -4.95 -12.05 -6.54 -8.29 -14.64 -10.07 -5.09 -9.16 -8.23 -20.54 -11.97 -13.55
Vacuole 1.49 -4.47 -3.32 -16.16 3.00 8.80 -12.49 -12.87 -16.48 1.18 8.69 -20.96 -17.08 -23.58 2.64
Vacuole Periphery 1.29 1.17 1.97 2.58 4.03 9.61 6.50 8.11 0.73 1.73 8.87 6.40 9.83 1.93 4.40
Standard name
Human Ortholog
Description Putative endonuclease; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
Localization
Cell Percentages mitochondrion (85%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Ngl1

Ngl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ngl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available