Standard name
Human Ortholog
Description Transcription factor involved in phospholipid synthesis; required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.1 0.08 0.1 – 0.18 0.15 0.18 0.16 0.13 0.09 0.16 0.2 0.14 0.18 0.18 0 0 0 0.06 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0 0.74 0.49 0.26 0.71 – 0.69 0.8 0.76 0.82 0.83 0.05 0.06 0.06 0.06 0.07 0.06 0 0 0 0 0 0
Nucleus 0.93 0.9 0.75 0.77 0.75 0.49 – 0.39 0.34 0.32 0.35 0.35 0.88 0.78 0.73 0.65 0.69 0.64 0.92 0.87 0.8 0.68 0.72 0.67
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0.05 – 0 0.06 0 0 0.05 0 0.05 0.09 0.23 0.17 0.15 0 0.06 0.1 0.1 0.17 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 1 1 8 – 13 20 19 24 22 0 1 0 0 0 0 0 1 1 3 3 4
Bud Neck 0 0 0 0 0 1 – 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 3 3 3 3 – 13 16 25 21 32 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 4 8 23 31 52 – 149 106 135 137 133 17 39 58 22 17 22 2 0 1 6 6 8
Endoplasmic Reticulum 1 0 1 0 0 0 – 1 0 0 0 0 0 2 0 5 0 3 0 0 0 0 1 0
Endosome 1 0 0 0 15 3 – 2 1 1 0 0 0 0 1 3 1 6 2 0 2 1 2 2
Golgi 0 0 0 1 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Mitochondria 55 2 159 116 101 360 – 587 552 564 710 847 9 15 19 9 7 7 0 1 3 1 2 3
Nucleus 222 76 161 181 288 247 – 330 231 235 301 356 161 186 217 101 66 77 224 76 178 70 188 178
Nuclear Periphery 0 1 1 0 1 0 – 3 1 5 3 6 0 1 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 2 4 4 – 7 3 4 0 6 0 0 3 0 0 0 0 0 2 0 3 5
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 3 4 – 13 6 6 12 5 0 0 1 0 0 2 2 0 4 2 5 4
Vac/Vac Membrane 4 4 3 4 9 24 – 34 39 18 39 48 5 11 26 35 16 18 7 5 22 10 44 53
Unique Cell Count 239 84 214 236 386 505 850 689 741 870 1024 184 237 297 155 96 120 243 87 222 103 264 266
Labelled Cell Count 287 88 337 331 457 706 1152 975 1013 1248 1455 192 255 326 175 107 135 243 87 222 103 264 266


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 5.7 3.5 4.2 4.8 3.4 – 3.5 2.8 3.7 2.8 2.8 5.4 5.4 5.0 6.9 6.0 7.0 4.7 5.3 5.2
Std Deviation (1e-4) 0.6 1.3 0.7 1.8 5.6 1.2 – 1.5 0.9 7.9 0.9 0.7 1.3 1.2 1.2 1.7 1.5 1.8 1.3 1.4 1.2
Intensity Change (Log2) – – – 0.27 0.44 -0.03 – 0.02 -0.33 0.09 -0.31 -0.32 0.62 0.63 0.52 0.98 0.77 0.99 0.41 0.59 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.5 2.0 2.9 – 5.1 4.5 5.2 4.6 3.9 2.3 4.4 5.3 3.6 4.2 4.5
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 -14.0 -14.8 -15.9 -13.0 -10.9 -12.0
Nucleus 0.4 -0.2 -6.5 – -9.5 -10.8 -11.4 -10.8 -10.9 3.1 0.8 -0.6 -2.1 -1.2 -2.1
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.2 – 1.9 2.6 0 2.1 2.2 0 2.0 3.5 6.6 5.2 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.7083 1.5457 1.4273 1.5349 1.4636 1.4361 0.5943 0.9674 0.6119 0.5736 0.0614 0.5244 3.053 2.8 2.2247 2.6749 2.4614 2.4487
Actin 0.0388 0.0002 0.0279 0.0133 0.0089 0.0062 0.0012 0.0001 0.0024 0 0.0008 0.0005 0.0216 0.0004 0.0064 0.0116 0.0166 0.0045
Bud 0.0007 0.0006 0.0006 0.0002 0.0001 0.0001 0.0003 0.0001 0.0004 0 0.0006 0 0.0004 0 0.0003 0.0005 0.0003 0.0001
Bud Neck 0.0033 0.0002 0.0011 0.0005 0.0008 0.0008 0.0009 0.0001 0.0002 0.0001 0.0005 0.0143 0.0055 0.0001 0.0004 0.0018 0.0004 0.0009
Bud Periphery 0.0021 0.0011 0.0011 0.0005 0.0002 0.0002 0.0009 0 0.0007 0 0.0054 0.0001 0.0006 0 0.0005 0.001 0.0004 0.0003
Bud Site 0.002 0.003 0.0019 0.0009 0.0004 0.0001 0.0012 0.0003 0.0004 0.0001 0.0006 0.0001 0.0089 0.0001 0.0027 0.0034 0.0013 0.0001
Cell Periphery 0.0003 0 0.0002 0.0002 0 0 0.0004 0 0.0001 0 0.0008 0 0.0003 0 0.0001 0.0002 0.0001 0.0001
Cytoplasm 0.0069 0.0021 0.0024 0.0001 0.0012 0.001 0.0195 0.0117 0.0043 0.0015 0.0003 0.0015 0.0092 0.0013 0.0043 0.001 0.0003 0.0003
Cytoplasmic Foci 0.0204 0.0001 0.0025 0.0013 0.005 0.0008 0.0066 0.0057 0.0029 0.0068 0.0001 0.0005 0.0041 0.0003 0.0025 0.006 0.0005 0.0001
Eisosomes 0.0003 0 0.0005 0.0005 0.0001 0 0 0 0 0 0.0002 0 0.0001 0 0.0001 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.0056 0.0001 0.0005 0.0001 0.0001 0.0002 0.0026 0.0004 0.001 0.0003 0.0004 0.0002 0.0047 0.0004 0.0008 0.0006 0 0.0008
Endosome 0.0244 0.0002 0.0095 0.003 0.0064 0.0015 0.0172 0.0038 0.0046 0.0616 0.0007 0.0017 0.0131 0.0033 0.0072 0.0105 0.0003 0.0016
Golgi 0.0109 0 0.0054 0.0061 0.0124 0.0019 0.0023 0.0003 0.0014 0.0111 0.0001 0.0034 0.0035 0.0002 0.0036 0.0068 0.001 0.0015
Lipid Particles 0.0175 0 0.0067 0.0073 0.0162 0.0004 0.0157 0.0003 0.0022 0.0002 0.0007 0.0028 0.0059 0.0001 0.0047 0.0145 0.0007 0.0007
Mitochondria 0.013 0.0017 0.0187 0.1003 0.004 0.0017 0.0415 0.0004 0.0022 0.0011 0.0978 0.0142 0.0023 0.0003 0.0107 0.0192 0.0174 0.002
None 0.0066 0.0016 0.0022 0.0001 0.0005 0.0002 0.0068 0.0181 0.0014 0 0.0004 0.0001 0.0066 0.0008 0.0025 0.0008 0.0001 0.0002
Nuclear Periphery 0.0359 0.0075 0.0043 0.0004 0.0015 0.0083 0.0167 0.0102 0.013 0.0023 0.0076 0.0111 0.0389 0.0028 0.0036 0.0074 0.0012 0.0077
Nucleolus 0.0079 0.005 0.0065 0.0052 0.0175 0.0097 0.009 0.0036 0.0045 0.0024 0.033 0.0071 0.0068 0.0034 0.0039 0.0071 0.0088 0.0109
Nucleus 0.7456 0.9714 0.8917 0.8512 0.8617 0.9488 0.828 0.9404 0.9358 0.9112 0.7798 0.9373 0.8488 0.9851 0.9387 0.8729 0.9462 0.9622
Peroxisomes 0.0236 0.0001 0.0061 0.0073 0.008 0.0009 0.0049 0.0002 0.001 0.0001 0.0004 0.0005 0.0027 0 0.002 0.0039 0.0028 0.0001
Punctate Nuclear 0.0187 0.0045 0.007 0.0005 0.0544 0.0169 0.0126 0.0033 0.0191 0.0002 0.0629 0.0041 0.0112 0.0009 0.0032 0.0286 0.0014 0.0051
Vacuole 0.0079 0.0003 0.0022 0.0004 0.0002 0.0002 0.0071 0.0007 0.0015 0.0005 0.0025 0.0002 0.0033 0.0003 0.0012 0.0011 0.0001 0.0002
Vacuole Periphery 0.0076 0.0001 0.0011 0.0005 0.0002 0.0002 0.0046 0.0003 0.0007 0.0005 0.0043 0.0003 0.0017 0.0002 0.0004 0.0009 0.0001 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.679 4.3262 12.8409 11.087 7.8433 14.5104 10.9356 12.5473 14.6403 11.8354
Translational Efficiency 2.4521 4.8608 1.1752 2.5014 2.8772 1.5216 2.0968 1.865 1.5701 2.2592

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transcription factor involved in phospholipid synthesis; required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ino4

Ino4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ino4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available