Standard name
Human Ortholog
Description Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0.05 0.06 0.07 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0.07 0.07 0.06 0.07 0.09 0.09 0.14 0.09 0.14 0 0.05 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.93 0.94 0.85 0.84 0.75 0.73 0.69 0.66 0.61 0.61 0.98 0.97 0.98 0.94 0.93 0.93 0.93 0.93 0.94 0.94 0.9 0.89
Endoplasmic Reticulum 0 0 0.06 0.05 0 0.05 0.11 0.11 0.15 0.17 0.11 0.19 0 0 0 0 0 0 0 0 0 0 0.06 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.08 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0 0 0.08 0.07 0.13 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.11 0 0.05 0.08 0.07 0 0.08 0.05 0 0 0 0 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 4 13 19 29 23 26 26 37 0 1 0 1 1 1 1 1 0 0 0 0
Bud 0 0 0 0 0 0 3 2 1 0 3 0 0 0 1 0 0 0 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 5 7 13 26 19 35 52 32 52 37 50 8 24 13 3 3 7 0 0 2 0 0 1
Cytoplasm 307 249 140 180 297 285 378 423 253 255 241 224 358 464 536 174 225 248 289 242 146 392 388 423
Endoplasmic Reticulum 7 3 9 9 10 17 55 61 55 66 45 70 9 17 6 7 5 9 9 6 3 16 28 27
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 3
Golgi 0 1 1 2 13 1 38 21 12 17 18 10 0 1 0 4 2 5 0 1 0 1 1 2
Mitochondria 0 1 0 1 3 27 10 13 29 26 51 33 0 0 1 2 5 1 0 0 0 1 1 1
Nucleus 0 0 1 0 0 1 1 5 0 1 1 1 0 0 0 1 0 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 1
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 38 14 23 47 25 13 33 17 0 1 0 7 17 12 3 2 1 1 4 2
Unique Cell Count 316 255 150 192 349 341 503 580 366 385 395 366 364 479 546 185 243 266 310 260 156 419 433 474
Labelled Cell Count 319 259 158 207 391 377 564 654 430 456 455 443 375 509 557 199 258 284 310 260 156 419 433 474


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.7 21.2 18.5 17.3 19.7 14.7 18.6 17.4 17.1 18.0 16.9 17.3 18.2 21.0 21.8 24.4 23.1 24.7 17.4 18.1 19.2
Std Deviation (1e-4) 2.9 3.9 3.0 2.8 5.9 3.8 3.5 3.5 3.7 4.0 4.1 4.1 3.3 4.1 4.1 6.8 6.6 6.0 2.9 3.4 3.7
Intensity Change (Log2) – – – -0.09 0.09 -0.34 0.0 -0.09 -0.11 -0.04 -0.13 -0.1 -0.02 0.18 0.23 0.4 0.32 0.41 -0.09 -0.03 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 2.8 3.1 3.3 3.2 4.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.8 1.1 0.4 1.0 1.7 1.6 2.9 1.8 3.0 0 0.2 0 0 0 -1.1
Cytoplasm 0.2 -2.6 -2.9 -4.8 -5.3 -5.9 -6.4 -7.3 -7.3 3.0 1.9 3.1 0.3 -0.3 0
Endoplasmic Reticulum -0.5 -1.7 -0.5 1.8 1.7 2.8 3.3 1.9 3.8 -2.0 -1.3 0 -0.9 -2.0 -1.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 3.1 0 0 2.2 2.2 0 0 0 0 0 0 0
Mitochondria 0 0 3.5 0 0 3.5 3.3 4.6 3.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.2 0 2.7 3.6 3.3 0 3.7 0 0 0 0 0 3.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.786 14.6225 14.0868 13.1055 11.7413 13.8 14.6223 20.7999 18.4995 18.4953 15.612 18.7037 17.7158 22.784 21.0821 19.6204 20.8354 21.0542
Actin 0.0175 0.0009 0.0015 0.0015 0.0104 0.0014 0.0187 0.0004 0.0018 0.0006 0.0064 0.0018 0.0007 0.0003 0.0006 0.0003 0.0002 0.004
Bud 0.0007 0.0003 0.001 0.0005 0.001 0.0003 0.0008 0.0001 0.0003 0.0003 0.0002 0.0001 0.0002 0.0001 0.0002 0.0003 0.0001 0.0007
Bud Neck 0.0008 0.0002 0.0004 0.0005 0.0008 0.001 0.0025 0.0002 0.0004 0.0004 0.0005 0.0006 0.0002 0.0002 0.0003 0.0003 0.0004 0.001
Bud Periphery 0.0008 0.0001 0.0012 0.0002 0.001 0.0002 0.0008 0 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0001 0 0 0.0012
Bud Site 0.0013 0.0005 0.0007 0.0007 0.0008 0.0001 0.0018 0.0002 0.0005 0.0002 0.0005 0.0001 0.0003 0.0003 0.0002 0.0002 0 0.0011
Cell Periphery 0.0004 0.0003 0.0002 0.0002 0.0003 0.0001 0.0004 0.0002 0.0001 0.0001 0.0002 0 0.0003 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.7675 0.9622 0.9371 0.8335 0.7432 0.899 0.7841 0.983 0.9293 0.9135 0.8422 0.9221 0.8941 0.946 0.9542 0.9671 0.9747 0.9129
Cytoplasmic Foci 0.0218 0.0048 0.0085 0.0425 0.0254 0.0132 0.0177 0.0029 0.0091 0.015 0.021 0.0078 0.0105 0.0295 0.0111 0.0048 0.0047 0.0087
Eisosomes 0.0001 0 0 0 0.0001 0 0.0002 0 0 0 0.0001 0 0 0 0 0 0 0.0001
Endoplasmic Reticulum 0.0542 0.0164 0.0118 0.0203 0.0173 0.0309 0.0532 0.0052 0.0147 0.0075 0.0133 0.0349 0.0551 0.0055 0.0163 0.0044 0.0081 0.0371
Endosome 0.0493 0.007 0.02 0.0485 0.0931 0.0318 0.0453 0.0031 0.02 0.0178 0.0497 0.0198 0.0182 0.0056 0.0075 0.0111 0.0063 0.0169
Golgi 0.0131 0.002 0.0018 0.0041 0.0237 0.0041 0.0073 0.0004 0.0032 0.001 0.0084 0.0037 0.002 0.003 0.001 0.0004 0.0003 0.0037
Lipid Particles 0.0088 0.0001 0.0003 0.0125 0.0038 0.002 0.0081 0.0001 0.0027 0.0017 0.0121 0.0007 0.0009 0.0004 0.0001 0.0001 0.0001 0.0002
Mitochondria 0.0125 0.001 0.0056 0.0021 0.059 0.0033 0.0095 0.0001 0.0015 0.0007 0.0147 0.0015 0.0007 0.0002 0.0002 0.0002 0.0001 0.003
None 0.0035 0.0004 0.0006 0.0015 0.0014 0.0005 0.0033 0.0003 0.0009 0.0072 0.0028 0.0006 0.0021 0.0003 0.0003 0.0004 0.0002 0.0011
Nuclear Periphery 0.0166 0.0007 0.0009 0.0044 0.0034 0.0017 0.0142 0.0006 0.0041 0.0038 0.0132 0.0014 0.005 0.0006 0.0015 0.0019 0.0009 0.0026
Nucleolus 0.0005 0 0 0.0002 0.0001 0 0.0006 0 0.0003 0.0001 0.0003 0 0.0001 0.0004 0 0 0 0.0003
Nucleus 0.0055 0.0006 0.0011 0.0014 0.002 0.0008 0.0082 0.0007 0.0032 0.0016 0.0022 0.0009 0.0018 0.0008 0.001 0.0012 0.0017 0.0014
Peroxisomes 0.0033 0 0.0004 0.0019 0.0015 0.0047 0.0037 0 0.0004 0.0004 0.0011 0.0003 0.0002 0.0005 0.0012 0 0 0.0002
Punctate Nuclear 0.0059 0.0001 0.0001 0.0011 0.0007 0.0011 0.0052 0 0.0004 0.0004 0.0025 0.0005 0.0008 0.0027 0.0001 0.0001 0.0001 0.0008
Vacuole 0.0124 0.0018 0.0055 0.0173 0.007 0.0027 0.0116 0.0024 0.0056 0.0241 0.0061 0.0022 0.0057 0.0031 0.0034 0.0062 0.0016 0.0018
Vacuole Periphery 0.0037 0.0003 0.0012 0.0051 0.0042 0.0012 0.0028 0.0002 0.0013 0.0036 0.0021 0.0008 0.001 0.0002 0.0006 0.0012 0.0005 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.1692 63.2886 26.345 43.0499 55.9707 32.7904 51.1148 52.6703 51.9627 44.1575
Translational Efficiency 3.5211 2.7198 4.0475 2.5238 2.6407 4.5545 2.2738 2.221 2.1468 2.4772

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Mdy2

Mdy2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdy2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available