Standard name | |
---|---|
Human Ortholog | |
Description | AdoMet-dependent tRNA methyltransferase; also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 1 | 1 | – | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 3 |
Bud | 0 | 3 | 6 | 0 | 1 | 1 | 7 | – | 4 | 10 | 11 | 9 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 17 | 4 | 7 | 7 | 8 | – | 5 | 9 | 3 | 4 | 1 | 4 | 1 | 10 | 14 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 171 | 206 | 302 | 292 | 396 | 358 | 587 | – | 307 | 314 | 328 | 378 | 365 | 479 | 539 | 397 | 219 | 158 | 161 | 207 | 305 | 227 | 302 | 341 |
Endoplasmic Reticulum | 4 | 1 | 0 | 1 | 0 | 4 | 3 | – | 7 | 1 | 0 | 5 | 3 | 1 | 1 | 20 | 5 | 3 | 0 | 0 | 1 | 6 | 10 | 8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 2 | 0 | 1 | 3 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 3 | 1 | 0 | 1 | 5 | 24 | 80 | – | 134 | 137 | 177 | 109 | 3 | 1 | 1 | 5 | 2 | 2 | 1 | 0 | 3 | 2 | 7 | 7 |
Nucleus | 0 | 0 | 1 | 0 | 3 | 4 | 4 | – | 2 | 4 | 4 | 4 | 2 | 2 | 0 | 12 | 1 | 8 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | 0 | 3 | 0 | 2 | 2 | – | 7 | 8 | 10 | 10 | 0 | 1 | 0 | 23 | 11 | 6 | 0 | 2 | 3 | 11 | 9 | 11 |
Unique Cell Count | 178 | 207 | 304 | 297 | 399 | 378 | 641 | 386 | 409 | 447 | 482 | 370 | 480 | 541 | 419 | 231 | 169 | 174 | 214 | 319 | 256 | 349 | 392 | |
Labelled Cell Count | 181 | 213 | 326 | 302 | 412 | 403 | 696 | 470 | 487 | 538 | 533 | 375 | 491 | 544 | 468 | 254 | 185 | 174 | 214 | 319 | 256 | 349 | 392 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.1 | 5.6 | 6.3 | 5.9 | 6.0 | 4.7 | 4.6 | – | 4.0 | 4.0 | 3.9 | 4.5 | 5.9 | 6.0 | 6.0 | 10.7 | 11.2 | 10.7 | 5.8 | 6.1 | 6.4 |
Std Deviation (1e-4) | 0.7 | 0.9 | 0.8 | 1.1 | 1.1 | 0.7 | 0.9 | – | 0.7 | 0.7 | 0.8 | 0.8 | 0.8 | 0.9 | 0.9 | 2.3 | 2.7 | 2.0 | 0.8 | 1.1 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.07 | -0.44 | -0.46 | – | -0.65 | -0.68 | -0.71 | -0.5 | -0.1 | -0.06 | -0.08 | 0.76 | 0.83 | 0.76 | -0.11 | -0.06 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.8 | -2.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | -4.3 | -4.0 | -5.2 | -2.2 | 0.2 | -0.4 |
Cytoplasm | -1.2 | -0.1 | -3.4 | -4.7 | – | -8.0 | -8.7 | -9.5 | -8.4 | -0.9 | 1.0 | 0.6 | -3.4 | -3.3 | -3.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7757 | 1.5456 | 1.3854 | 0.8114 | 1.0454 | 1.2008 | 1.0416 | 2.0317 | 1.7021 | 1.7 | 1.0476 | 1.7702 | 1.2423 | 2.3479 | 1.8728 | 2.0984 | 1.7082 | 2.0849 |
Actin | 0.045 | 0.001 | 0.0228 | 0.0115 | 0.0002 | 0.01 | 0.0047 | 0.0005 | 0.002 | 0.0315 | 0.0009 | 0.0075 | 0.0218 | 0.0027 | 0.0172 | 0.0029 | 0 | 0.0152 |
Bud | 0.001 | 0.0005 | 0.0011 | 0.0015 | 0.0002 | 0.0008 | 0.0005 | 0.0004 | 0.0035 | 0.0067 | 0.0003 | 0.0007 | 0.0004 | 0.0002 | 0.0009 | 0.0003 | 0.0001 | 0.0002 |
Bud Neck | 0.0158 | 0.0002 | 0.0021 | 0.0009 | 0.0004 | 0.0024 | 0.0016 | 0.0002 | 0.0012 | 0.0006 | 0.0004 | 0.0006 | 0.0002 | 0.0001 | 0.001 | 0.0002 | 0.0001 | 0.0006 |
Bud Periphery | 0.0023 | 0.0004 | 0.0008 | 0.002 | 0.0002 | 0.0009 | 0.0005 | 0.0001 | 0.0021 | 0.0067 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0007 | 0.0002 | 0 | 0.0002 |
Bud Site | 0.0032 | 0.0023 | 0.0092 | 0.001 | 0.0002 | 0.0005 | 0.0022 | 0.0036 | 0.0027 | 0.0076 | 0.0003 | 0.0002 | 0.0007 | 0.0009 | 0.0033 | 0.0007 | 0 | 0.0001 |
Cell Periphery | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 |
Cytoplasm | 0.5839 | 0.743 | 0.73 | 0.8507 | 0.7738 | 0.7662 | 0.7331 | 0.8885 | 0.8121 | 0.7804 | 0.8423 | 0.9006 | 0.7075 | 0.9052 | 0.8529 | 0.8706 | 0.8927 | 0.8901 |
Cytoplasmic Foci | 0.0281 | 0.0214 | 0.0306 | 0.0181 | 0.0225 | 0.0172 | 0.0499 | 0.0253 | 0.0319 | 0.0408 | 0.0105 | 0.0174 | 0.015 | 0.0057 | 0.0191 | 0.0081 | 0.0072 | 0.0126 |
Eisosomes | 0.0004 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0077 | 0.0042 | 0.0037 | 0.0024 | 0.0031 | 0.0027 | 0.0064 | 0.0011 | 0.0021 | 0.0036 | 0.0011 | 0.0021 | 0.0074 | 0.0025 | 0.0037 | 0.0086 | 0.0025 | 0.0029 |
Endosome | 0.0208 | 0.0067 | 0.0107 | 0.013 | 0.0199 | 0.0102 | 0.0526 | 0.0133 | 0.0314 | 0.0301 | 0.0498 | 0.0134 | 0.0264 | 0.0033 | 0.0155 | 0.02 | 0.0068 | 0.0101 |
Golgi | 0.0092 | 0.0021 | 0.0026 | 0.0031 | 0.0006 | 0.0016 | 0.0121 | 0.0015 | 0.0115 | 0.0031 | 0.0253 | 0.0045 | 0.0056 | 0.0005 | 0.0069 | 0.0046 | 0.0001 | 0.0046 |
Lipid Particles | 0.0056 | 0.0013 | 0.0021 | 0.0006 | 0.0006 | 0.0007 | 0.0114 | 0.0015 | 0.0068 | 0.0056 | 0.0022 | 0.0036 | 0.0069 | 0.0002 | 0.0027 | 0.0005 | 0.0001 | 0.0062 |
Mitochondria | 0.0068 | 0.0006 | 0.0015 | 0.0024 | 0.0005 | 0.0013 | 0.0114 | 0.0004 | 0.0163 | 0.0011 | 0.0502 | 0.0022 | 0.0021 | 0.0041 | 0.0113 | 0.0019 | 0.0001 | 0.0036 |
None | 0.2435 | 0.2027 | 0.1596 | 0.0577 | 0.146 | 0.1749 | 0.0658 | 0.0581 | 0.0656 | 0.0478 | 0.0084 | 0.0402 | 0.1821 | 0.0707 | 0.0538 | 0.0747 | 0.0864 | 0.0491 |
Nuclear Periphery | 0.0028 | 0.0009 | 0.0023 | 0.0008 | 0.0015 | 0.0025 | 0.0116 | 0.0002 | 0.0009 | 0.001 | 0.0002 | 0.0005 | 0.0058 | 0.0009 | 0.0019 | 0.0021 | 0.0005 | 0.0004 |
Nucleolus | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Nucleus | 0.002 | 0.0014 | 0.0056 | 0.0016 | 0.0022 | 0.0026 | 0.0108 | 0.0009 | 0.0011 | 0.0016 | 0.0006 | 0.0009 | 0.0038 | 0.0008 | 0.0011 | 0.0011 | 0.0005 | 0.0008 |
Peroxisomes | 0.0151 | 0.007 | 0.0086 | 0.0276 | 0.0003 | 0.0007 | 0.0081 | 0.0023 | 0.0034 | 0.023 | 0.0026 | 0.0023 | 0.0025 | 0.0001 | 0.0033 | 0.0001 | 0 | 0.0017 |
Punctate Nuclear | 0.0019 | 0.0011 | 0.0027 | 0.0005 | 0.024 | 0.002 | 0.0081 | 0.0004 | 0.001 | 0.0013 | 0.0001 | 0.0006 | 0.0064 | 0.0002 | 0.0017 | 0.0005 | 0.0002 | 0.0004 |
Vacuole | 0.0033 | 0.0024 | 0.0028 | 0.0039 | 0.0028 | 0.0021 | 0.0061 | 0.0013 | 0.0033 | 0.0059 | 0.0029 | 0.0015 | 0.0034 | 0.0012 | 0.0019 | 0.0021 | 0.0024 | 0.0009 |
Vacuole Periphery | 0.0008 | 0.0003 | 0.0006 | 0.0006 | 0.0006 | 0.0005 | 0.0019 | 0.0002 | 0.0009 | 0.0012 | 0.0015 | 0.0004 | 0.0009 | 0.0003 | 0.0008 | 0.0006 | 0.0004 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.1177 | 8.5032 | 8.7636 | 8.4983 | 7.8793 | 11.0913 | 8.2394 | 6.2864 | 6.1422 | 10.7323 |
Translational Efficiency | 0.8467 | 0.95 | 1.0974 | 0.8213 | 1.2575 | 0.82 | 0.8154 | 0.8219 | 0.7553 | 0.9845 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|