ORF
Human Ortholog
Description Protein of unknown function; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YOR062C has a paralog, YKR075C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.25 0.44 0.28 0.17 0.08 0.06 – 0 0.05 0 0 0.32 0.49 0.48 0.41 0.3 0.26 0.22 0.15 0.23 0.23 0.33 0.48
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0 0
Endosome 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.13 0 0.06 0.14 0.09 0.05 – 0.31 0.16 0.17 0.2 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.48 0.42 0.4 0.59 0.71 0.84 0.9 – 0.78 0.86 0.87 0.85 0.52 0.35 0.28 0.32 0.39 0.51 0.31 0.57 0.4 0.42 0.26 0.15
Nuclear Periphery 0.12 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0.09 0.05 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Vac/Vac Membrane 0.22 0.36 0.19 0.09 0.07 0.06 0 – 0 0 0 0 0.19 0.27 0.34 0.32 0.37 0.27 0.34 0.19 0.23 0.22 0.22 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 4
Bud 1 1 0 0 2 0 2 – 6 3 4 8 0 0 0 0 0 0 1 5 4 7 12 20
Bud Neck 1 0 2 0 2 0 0 – 0 1 1 1 0 0 0 0 0 0 0 1 1 2 7 5
Bud Site 0 0 0 0 1 3 0 – 14 8 8 14 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0
Cytoplasm 24 60 121 60 51 28 30 – 16 20 8 10 99 201 198 193 86 86 42 43 85 69 120 230
Endoplasmic Reticulum 0 1 1 0 0 0 0 – 0 0 0 0 3 2 2 38 14 12 5 0 1 3 2 5
Endosome 7 5 2 5 0 3 2 – 1 0 0 0 3 7 12 12 16 4 2 2 3 3 7 8
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 2 2 0 2 0 0 2 0 0 1
Mitochondria 12 31 3 12 44 30 23 – 126 63 71 96 0 1 0 28 10 12 3 4 15 7 7 14
Nucleus 58 102 110 126 216 288 452 – 321 349 356 405 160 144 117 152 113 167 60 164 146 129 94 70
Nuclear Periphery 15 0 0 1 0 0 0 – 4 2 1 2 0 0 0 0 0 2 1 0 0 0 1 1
Nucleolus 5 0 0 0 0 1 2 – 35 20 47 44 0 0 0 0 0 0 0 0 0 0 2 2
Peroxisomes 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 1 5 2 4 – 1 0 2 0 1 1 0 2 0 0 5 6 9 8 16 16
Vac/Vac Membrane 26 87 53 20 22 22 12 – 11 5 6 8 59 110 139 150 106 88 68 54 83 67 79 81
Unique Cell Count 120 243 276 212 305 341 504 410 405 411 474 305 410 411 474 289 330 198 287 368 309 365 479
Labelled Cell Count 150 287 294 225 343 377 527 536 471 504 588 326 467 470 577 346 373 198 287 368 309 365 479


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.3 5.4 5.3 4.7 5.4 5.8 – 5.4 5.9 6.0 6.0 6.3 6.4 6.7 4.9 6.4 6.3 6.6 7.0 7.1
Std Deviation (1e-4) 0.5 0.6 1.2 1.2 1.2 1.3 1.3 – 1.6 1.4 1.7 1.6 1.4 1.4 1.8 1.1 1.6 1.1 1.7 2.0 2.2
Intensity Change (Log2) – – – -0.04 -0.21 -0.0 0.09 – -0.0 0.13 0.14 0.15 0.23 0.25 0.3 -0.13 0.24 0.23 0.29 0.38 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.7 4.6 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4653 0.5518 0.4423 0.1375 0.0173 0.7039 5.1085 4.5135 4.3175 4.1834 4.8417 4.5694 2.2636 2.5242 2.4469 1.6889 2.08 2.5573
Actin 0.0004 0.0001 0.0002 0 0.0003 0.0002 0.0358 0.0005 0.0078 0.0534 0.0149 0.0009 0.0347 0.0002 0.0051 0.013 0.0088 0.0032
Bud 0 0.0001 0 0 0.0001 0 0.0004 0.0015 0.0003 0.0071 0.0012 0.0009 0.0002 0.0006 0.0003 0.0006 0.0005 0.0001
Bud Neck 0.0007 0.0003 0.0003 0.0003 0.0008 0.0015 0.0019 0.0008 0.0012 0.018 0.0019 0.0039 0.0027 0.0005 0.0027 0.003 0.0041 0.0026
Bud Periphery 0 0 0 0 0.0001 0 0.0006 0.0016 0.0004 0.0159 0.0009 0.0021 0.0002 0.0005 0.0003 0.0012 0.0007 0.0001
Bud Site 0.0002 0.0004 0.0002 0.0001 0.0003 0 0.0034 0.0025 0.0029 0.0057 0.0118 0.0018 0.0012 0.0022 0.0062 0.0122 0.0098 0.0001
Cell Periphery 0 0 0 0 0 0 0.0002 0.0001 0.0001 0.0007 0.0001 0.0003 0.0002 0.0001 0.0002 0.0008 0.0002 0
Cytoplasm 0.0353 0.0683 0.0459 0.0491 0.12 0.0292 0.1108 0.2244 0.1433 0.0947 0.1965 0.1098 0.0775 0.0844 0.0888 0.1428 0.0703 0.0496
Cytoplasmic Foci 0.0047 0.0023 0.0015 0.0004 0.0043 0.001 0.0128 0.0045 0.0121 0.0079 0.0245 0.0055 0.005 0.0017 0.0023 0.0031 0.0025 0.002
Eisosomes 0 0.0001 0 0 0 0 0.0003 0 0.0003 0.0003 0.0001 0 0.0004 0 0.0001 0.0007 0.0005 0
Endoplasmic Reticulum 0.0017 0.0033 0.0014 0.0008 0.0031 0.0013 0.0076 0.0041 0.0033 0.0034 0.0036 0.0013 0.0063 0.0026 0.0014 0.0027 0.0025 0.0024
Endosome 0.0042 0.0024 0.0015 0.0007 0.0065 0.0016 0.0255 0.0066 0.0099 0.0135 0.0187 0.0021 0.0107 0.0048 0.0016 0.0097 0.004 0.0069
Golgi 0.0005 0 0 0 0.0001 0.0001 0.0051 0.0007 0.0014 0.0069 0.0259 0.0002 0.004 0.0001 0.0003 0.0041 0.0013 0.001
Lipid Particles 0.005 0.0005 0.0001 0 0.0001 0.0003 0.0108 0.0003 0.0039 0.0054 0.014 0.0002 0.008 0.0002 0.0003 0.0087 0.0027 0.0009
Mitochondria 0.0004 0.0003 0.0003 0.0002 0.0014 0.0003 0.0053 0.0009 0.0032 0.0096 0.0034 0.0004 0.001 0.0006 0.0006 0.0045 0.0022 0.0008
None 0.0249 0.1342 0.064 0.0784 0.0458 0.0381 0.202 0.2365 0.203 0.1581 0.1604 0.1668 0.1354 0.1663 0.1609 0.1543 0.0754 0.0931
Nuclear Periphery 0.014 0.0238 0.0165 0.0052 0.0189 0.0123 0.0406 0.0165 0.0145 0.0206 0.0247 0.0073 0.0147 0.0116 0.0101 0.0059 0.008 0.0165
Nucleolus 0.0038 0.0042 0.0036 0.0025 0.0061 0.006 0.0061 0.0033 0.0034 0.0057 0.0019 0.0053 0.0042 0.004 0.004 0.0032 0.015 0.0067
Nucleus 0.8663 0.73 0.834 0.8562 0.7387 0.8826 0.4738 0.4572 0.5474 0.4878 0.4214 0.6266 0.6661 0.6973 0.684 0.6177 0.7176 0.778
Peroxisomes 0.0022 0 0 0 0.0003 0 0.0057 0.0003 0.0023 0.0019 0.0115 0.0008 0.0058 0.0001 0.0002 0.002 0.0017 0.0002
Punctate Nuclear 0.0341 0.0273 0.0291 0.0051 0.0492 0.0245 0.0473 0.0327 0.0371 0.0785 0.0603 0.062 0.0196 0.0186 0.0287 0.0061 0.07 0.0345
Vacuole 0.001 0.0018 0.001 0.0008 0.0028 0.0005 0.0025 0.0044 0.0016 0.0039 0.0016 0.0017 0.0017 0.0032 0.0017 0.0029 0.0019 0.0009
Vacuole Periphery 0.0003 0.0004 0.0003 0.0001 0.0008 0.0004 0.0015 0.0006 0.0005 0.001 0.0008 0.0002 0.0004 0.0004 0.0003 0.0009 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.1528 4.8891 3.6279 7.6774 6.2046 9.5657 8.9598 8.3411 7.6195 7.56
Translational Efficiency 0.6815 0.7709 1.1531 0.5058 0.4572 0.8352 1.0146 0.7793 1.1578 0.6268

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
322 950 633 1330 2273 2102 1457 1597 2595 3052 2090 2927

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 615.32 736.38 945.93 853.09 710.10 814.97 875.88 841.78 698.34 790.51 897.10 846.92
Standard Deviation 73.33 106.97 128.66 112.00 80.76 91.37 114.61 130.98 85.77 103.13 123.31 122.85
Intensity Change Log 2 — 0.259114 0.620397 0.471361 — 0.198725 0.302711 0.245421 — 0.227075 0.458923 0.354725

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000064 0.000421 0.000630 0.000311 0.000017 0.000081 0.000239 0.000713 0.000023 0.000187 0.000357 0.000530
Bud Neck 0.005092 0.013859 0.009628 0.013485 0.002507 0.016005 0.005076 0.008506 0.002828 0.015337 0.006455 0.010768
Bud Site 0.001338 0.006496 0.004600 0.013826 0.000553 0.006855 0.002871 0.011952 0.000650 0.006743 0.003395 0.012803
Cell Periphery 0.000398 0.000193 0.000088 0.000361 0.000127 0.000286 0.000188 0.000229 0.000160 0.000257 0.000157 0.000289
Cytoplasm 0.211494* 0.231432* 0.015406 0.255750* 0.218137* 0.242524* 0.062386 0.134186* 0.217313* 0.239072* 0.048157 0.189423*
Cytoplasmic Foci 0.083862 0.067671 0.000128 0.008158 0.018312 0.041490 0.002088 0.001378 0.026446 0.049639 0.001495 0.004459
Eisosomes 0.000105 0.000073 0.000025 0.000048 0.000020 0.000059 0.000027 0.000023 0.000030 0.000063 0.000027 0.000035
Endoplasmic Reticulum 0.000853 0.003055 0.007151 0.002489 0.000644 0.000824 0.002574 0.004583 0.000670 0.001518 0.003960 0.003632
Endosome 0.000922 0.005195 0.000484 0.002726 0.000541 0.005525 0.000522 0.001388 0.000588 0.005422 0.000510 0.001996
Golgi 0.000382 0.001420 0.000048 0.000453 0.000081 0.000901 0.000049 0.000192 0.000118 0.001062 0.000049 0.000310
Lipid Particles 0.006961 0.005158 0.000235 0.001078 0.001247 0.004810 0.000442 0.000329 0.001956 0.004918 0.000379 0.000669
Mitochondria 0.004736 0.006777 0.000825 0.003533 0.000490 0.006006 0.000990 0.001764 0.001017 0.006246 0.000940 0.002568
Mitotic Spindle 0.000103 0.003057 0.001292 0.025125 0.000026 0.004344 0.002070 0.008461 0.000036 0.003944 0.001834 0.016033
None 0.027333 0.010166 0.002954 0.018107 0.006360 0.011498 0.009307 0.018564 0.008963 0.011083 0.007383 0.018357
Nuclear Periphery 0.000656 0.001478 0.000966 0.001949 0.000980 0.001134 0.000855 0.001283 0.000940 0.001241 0.000889 0.001586
Nuclear Periphery Foci 0.000175 0.000845 0.000219 0.001279 0.000129 0.000244 0.000140 0.000462 0.000135 0.000431 0.000164 0.000833
Nucleolus 0.002949 0.004341 0.001076 0.002998 0.001485 0.004351 0.001598 0.001084 0.001667 0.004348 0.001440 0.001954
Nucleus 0.643051* 0.599589* 0.947369* 0.609360* 0.744433* 0.629110* 0.903799* 0.792482* 0.731853* 0.619921* 0.916995* 0.709273*
Peroxisomes 0.004599 0.008024 0.000099 0.000882 0.000461 0.003419 0.000239 0.000238 0.000974 0.004852 0.000197 0.000531
Vacuole 0.004610 0.029840 0.006662 0.036449 0.003370 0.019558 0.004453 0.011981 0.003523 0.022759 0.005122 0.023099
Vacuole Periphery 0.000318 0.000910 0.000116 0.001633 0.000079 0.000976 0.000088 0.000203 0.000109 0.000956 0.000096 0.000853

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.21 -15.13 -11.58 0.27 7.07 -5.50 -15.72 -6.13 -5.57 -3.65 -8.38 -21.88 -8.60 -5.87 -2.06
Bud Neck -5.16 -3.83 -4.20 1.01 -0.37 -8.73 -6.45 -8.79 3.17 -3.84 -10.60 -8.06 -9.94 3.09 -3.27
Bud Site -6.07 -2.41 -5.86 -2.15 -2.44 -6.36 -2.74 -6.62 -2.19 -4.56 -8.28 -3.81 -9.07 -2.91 -5.38
Cell Periphery 3.28 4.94 2.40 -1.60 -5.41 -5.75 -2.16 -3.53 3.25 -1.40 -4.34 0.72 -2.76 1.88 -4.06
Cytoplasm -1.13 15.64 0.41 2.22 -28.23 -3.52 27.78 15.16 17.93 -9.67 -3.40 34.16 10.18 13.91 -23.67
Cytoplasmic Foci 1.65 11.07 10.33 15.29 -8.15 -9.95 12.94 13.34 20.15 1.32 -9.90 16.62 15.12 24.55 -5.52
Eisosomes 2.10 5.49 3.84 4.99 -7.31 -15.29 -5.15 -6.51 10.15 -1.31 -12.22 0.26 -5.00 9.53 -7.09
Endoplasmic Reticulum -9.54 -14.97 -13.83 -4.24 7.42 -1.88 -13.33 -19.75 -19.13 -7.81 -8.46 -19.44 -24.15 -18.65 -0.76
Endosome -5.44 2.49 -4.47 3.71 -5.97 -7.81 -0.02 -1.74 5.76 -1.71 -9.59 0.38 -4.20 6.79 -4.30
Golgi -1.93 3.82 1.12 2.18 -2.89 -4.20 1.39 -1.21 3.69 -1.80 -4.47 3.04 -1.70 4.00 -3.33
Lipid Particles 1.59 6.75 5.90 7.23 -9.20 -7.84 6.40 6.60 10.04 -0.45 -7.69 9.31 7.12 11.99 -6.67
Mitochondria -1.19 3.31 1.67 3.17 -2.85 -6.85 -2.03 -7.54 5.02 -2.56 -7.72 -0.06 -3.85 5.60 -3.55
Mitotic Spindle -3.18 -1.78 -7.57 -6.12 -6.55 -4.45 -3.24 -6.92 -3.60 -4.38 -5.37 -3.71 -10.19 -6.82 -7.66
None 3.59 5.25 3.28 -0.79 -6.01 -3.73 -1.87 -4.45 -1.01 -3.17 -1.52 2.51 -2.62 -1.09 -5.16
Nuclear Periphery -6.45 -4.89 -12.20 -7.35 -8.18 -1.50 -1.20 -6.12 -5.57 -5.09 -3.24 -2.25 -11.19 -9.75 -9.54
Nuclear Periphery Foci -4.75 -2.28 -3.36 -1.18 -3.00 -1.74 -0.71 -3.11 -2.55 -3.01 -4.45 -1.66 -4.58 -2.98 -4.33
Nucleolus -2.33 5.98 0.54 2.13 -2.25 -4.97 0.03 1.17 6.93 2.04 -5.71 0.99 -0.16 5.55 -1.20
Nucleus 2.25 -16.56 0.02 -3.42 31.49 13.64 -23.26 -6.67 -18.39 13.07 14.93 -29.71 0.49 -14.15 29.35
Peroxisomes -1.94 3.51 3.17 6.17 -3.78 -6.78 1.98 2.44 7.49 0.02 -7.61 4.19 3.36 9.38 -1.82
Vacuole -10.29 -3.67 -16.91 -8.04 -14.63 -12.50 -3.99 -11.69 -1.06 -8.84 -16.31 -5.76 -20.07 -8.76 -17.19
Vacuole Periphery -5.50 2.73 -1.83 0.07 -2.52 -7.84 -1.23 -5.72 6.77 -4.61 -10.14 0.47 -2.90 2.83 -2.95
ORF
Human Ortholog
Description Protein of unknown function; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YOR062C has a paralog, YKR075C, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (46%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

YOR062C

YOR062C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yor062c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available