Standard name | |
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Human Ortholog | |
Description | Cis-golgi GTPase-activating protein (GAP) for yeast Rabs; the Rab family members are Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 9 | 11 | 5 | 10 | 12 | 13 | – | 11 | 16 | 9 | 17 | 1 | 7 | 2 | 3 | 5 | 2 | 12 | 11 | 6 | 2 | 5 | 7 |
Bud | 3 | 2 | 0 | 1 | 0 | 4 | 13 | – | 9 | 9 | 12 | 16 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 4 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 3 | 2 | 4 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 2 | 1 | 3 | 2 | 3 | – | 4 | 12 | 4 | 8 | 4 | 2 | 0 | 7 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 133 | 217 | 143 | 144 | 188 | 139 | 193 | – | 90 | 141 | 111 | 134 | 58 | 204 | 220 | 138 | 108 | 113 | 87 | 166 | 96 | 92 | 277 | 246 |
Endoplasmic Reticulum | 10 | 17 | 5 | 9 | 15 | 19 | 6 | – | 14 | 24 | 19 | 30 | 3 | 11 | 2 | 7 | 3 | 5 | 5 | 6 | 9 | 0 | 2 | 4 |
Endosome | 31 | 9 | 7 | 10 | 26 | 26 | 58 | – | 24 | 38 | 12 | 32 | 0 | 13 | 20 | 18 | 13 | 6 | 57 | 30 | 11 | 7 | 11 | 12 |
Golgi | 0 | 1 | 0 | 0 | 2 | 1 | 3 | – | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 11 | 5 | 4 | 0 | 1 | 1 |
Mitochondria | 11 | 4 | 0 | 7 | 3 | 33 | 61 | – | 63 | 84 | 172 | 118 | 0 | 0 | 0 | 3 | 1 | 2 | 27 | 40 | 51 | 16 | 19 | 22 |
Nucleus | 0 | 2 | 1 | 1 | 1 | 4 | 1 | – | 0 | 0 | 2 | 4 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 8 | – | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 2 | 4 | 6 | 4 | 14 | – | 6 | 4 | 3 | 20 | 0 | 5 | 3 | 2 | 0 | 1 | 5 | 5 | 2 | 2 | 3 | 3 |
Vac/Vac Membrane | 8 | 5 | 6 | 1 | 7 | 6 | 20 | – | 9 | 18 | 8 | 21 | 0 | 4 | 10 | 21 | 20 | 12 | 9 | 19 | 12 | 4 | 11 | 5 |
Unique Cell Count | 191 | 255 | 168 | 172 | 245 | 223 | 345 | 194 | 299 | 288 | 331 | 61 | 237 | 251 | 172 | 136 | 130 | 226 | 296 | 209 | 135 | 349 | 319 | |
Labelled Cell Count | 207 | 268 | 178 | 184 | 261 | 250 | 394 | 231 | 350 | 355 | 405 | 67 | 248 | 257 | 203 | 153 | 146 | 226 | 296 | 209 | 135 | 349 | 319 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 6.6 | 7.2 | 6.7 | 7.5 | 6.4 | 6.6 | – | 6.0 | 6.5 | 5.5 | 5.9 | 6.9 | 6.9 | 6.9 | 9.6 | 9.7 | 9.1 | 6.3 | 6.7 | 7.0 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.1 | 1.9 | 1.5 | 1.1 | 1.4 | – | 1.2 | 1.7 | 1.0 | 1.3 | 0.9 | 0.9 | 1.2 | 2.4 | 2.4 | 2.1 | 0.9 | 1.0 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.04 | -0.18 | -0.13 | – | -0.26 | -0.16 | -0.4 | -0.3 | -0.06 | -0.06 | -0.08 | 0.41 | 0.42 | 0.33 | -0.2 | -0.12 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.6 | -1.1 | -0.5 | -1.4 | – | -0.3 | -0.5 | -1.7 | -0.6 | 0 | -1.7 | -3.3 | -2.2 | -1.1 | -2.1 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.4 | -2.1 | -5.0 | -6.5 | – | -7.7 | -8.1 | -9.7 | -9.5 | 2.0 | 0.3 | 0.7 | -1.2 | -1.3 | 0.4 |
Endoplasmic Reticulum | 1.0 | 1.5 | 2.3 | 0 | – | 1.8 | 2.2 | 1.7 | 2.5 | 0 | 0.8 | 0 | 0.5 | 0 | 0 |
Endosome | 0.7 | 2.4 | 2.6 | 4.0 | – | 2.8 | 3.0 | 0 | 2.2 | 0 | 0.6 | 1.6 | 2.2 | 1.9 | 0.2 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.2 | 5.8 | – | 8.1 | 7.6 | 12.7 | 8.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1.8 | – | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | -0.4 | -0.5 | 1.1 | – | 0.5 | 1.2 | -0.5 | 1.3 | 0 | 0 | 0.2 | 2.9 | 3.5 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.3521 | 0.6112 | 0.3857 | 0.1421 | -0.9705 | 0.3404 | 1.7031 | 2.1432 | 1.9442 | 1.8845 | 1.8172 | 1.8335 | 0.7684 | 0.6781 | 0.2492 | 1.0332 | 0.4227 | 0.6462 |
Actin | 0.0083 | 0.0085 | 0.0085 | 0.002 | 0.0024 | 0.0008 | 0.0228 | 0.0045 | 0.0412 | 0.0131 | 0.027 | 0.0108 | 0.0745 | 0.0012 | 0.0016 | 0.0508 | 0.0783 | 0.0144 |
Bud | 0.0006 | 0.002 | 0.0007 | 0.0003 | 0.0004 | 0.0001 | 0.0041 | 0.0009 | 0.0045 | 0.001 | 0.0008 | 0.0057 | 0.0006 | 0.0004 | 0.0004 | 0.0002 | 0.0005 | 0.0002 |
Bud Neck | 0.0052 | 0.0037 | 0.0061 | 0.0013 | 0.0002 | 0.0006 | 0.0021 | 0.0013 | 0.0032 | 0.0006 | 0.0007 | 0.0041 | 0.0007 | 0.0002 | 0.0002 | 0.0003 | 0.001 | 0.0009 |
Bud Periphery | 0.0008 | 0.0016 | 0.001 | 0.0006 | 0.0005 | 0.0001 | 0.0084 | 0.0008 | 0.0048 | 0.0012 | 0.0007 | 0.0028 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0009 | 0.0003 |
Bud Site | 0.0084 | 0.0228 | 0.0028 | 0.0013 | 0.0015 | 0.0001 | 0.0125 | 0.0107 | 0.007 | 0.0021 | 0.0013 | 0.0005 | 0.002 | 0.0097 | 0.0006 | 0.0014 | 0.0015 | 0.0003 |
Cell Periphery | 0.0013 | 0.0006 | 0.0005 | 0.0012 | 0.0003 | 0.0001 | 0.0008 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0001 |
Cytoplasm | 0.2304 | 0.4443 | 0.4136 | 0.4076 | 0.2054 | 0.409 | 0.3244 | 0.5635 | 0.4544 | 0.3283 | 0.3788 | 0.5107 | 0.3209 | 0.6096 | 0.4725 | 0.2472 | 0.254 | 0.5203 |
Cytoplasmic Foci | 0.1419 | 0.0967 | 0.0707 | 0.1439 | 0.0848 | 0.1636 | 0.121 | 0.0818 | 0.1174 | 0.1561 | 0.1451 | 0.1191 | 0.072 | 0.0797 | 0.0908 | 0.0576 | 0.1469 | 0.1087 |
Eisosomes | 0.0015 | 0.0004 | 0.0011 | 0.0005 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0003 | 0.0013 | 0.0002 |
Endoplasmic Reticulum | 0.0095 | 0.0067 | 0.0076 | 0.0064 | 0.0087 | 0.0056 | 0.0141 | 0.0088 | 0.0067 | 0.0069 | 0.0088 | 0.009 | 0.0112 | 0.0062 | 0.0122 | 0.0176 | 0.0094 | 0.006 |
Endosome | 0.137 | 0.1222 | 0.1594 | 0.069 | 0.1297 | 0.1948 | 0.1318 | 0.0906 | 0.1394 | 0.2087 | 0.1861 | 0.1356 | 0.1594 | 0.1043 | 0.2221 | 0.1591 | 0.2141 | 0.1559 |
Golgi | 0.029 | 0.0115 | 0.0148 | 0.0067 | 0.0044 | 0.0162 | 0.0235 | 0.015 | 0.0244 | 0.0241 | 0.0444 | 0.0179 | 0.0177 | 0.0105 | 0.0132 | 0.0158 | 0.0535 | 0.0229 |
Lipid Particles | 0.042 | 0.0033 | 0.0105 | 0.0025 | 0.003 | 0.0072 | 0.0208 | 0.0059 | 0.0066 | 0.004 | 0.0097 | 0.003 | 0.015 | 0.0014 | 0.0022 | 0.0357 | 0.0889 | 0.0081 |
Mitochondria | 0.0119 | 0.0211 | 0.0095 | 0.0024 | 0.0025 | 0.003 | 0.0171 | 0.0149 | 0.0079 | 0.0075 | 0.0182 | 0.0148 | 0.0099 | 0.0041 | 0.0068 | 0.0036 | 0.0203 | 0.0057 |
None | 0.3127 | 0.2343 | 0.2643 | 0.3416 | 0.5435 | 0.1812 | 0.2181 | 0.1856 | 0.1455 | 0.2053 | 0.1459 | 0.151 | 0.2566 | 0.1588 | 0.1596 | 0.3795 | 0.0122 | 0.1437 |
Nuclear Periphery | 0.0031 | 0.0014 | 0.002 | 0.001 | 0.0011 | 0.0007 | 0.0184 | 0.0012 | 0.003 | 0.0038 | 0.002 | 0.0035 | 0.0162 | 0.0014 | 0.0024 | 0.0052 | 0.0152 | 0.0021 |
Nucleolus | 0.0048 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0012 | 0.0002 | 0.0014 | 0.0007 | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0.0001 | 0.0002 | 0.001 | 0.0001 |
Nucleus | 0.0028 | 0.001 | 0.0009 | 0.0013 | 0.0009 | 0.0006 | 0.017 | 0.0011 | 0.0092 | 0.0131 | 0.0011 | 0.0011 | 0.0063 | 0.0011 | 0.0008 | 0.0009 | 0.0022 | 0.0007 |
Peroxisomes | 0.0155 | 0.0077 | 0.0108 | 0.0018 | 0.0008 | 0.0059 | 0.0263 | 0.002 | 0.0103 | 0.0043 | 0.0176 | 0.0036 | 0.0088 | 0.0012 | 0.0009 | 0.0097 | 0.0453 | 0.0028 |
Punctate Nuclear | 0.0094 | 0.0012 | 0.0027 | 0.0014 | 0.0011 | 0.0009 | 0.0039 | 0.0007 | 0.0031 | 0.0052 | 0.0012 | 0.0009 | 0.0179 | 0.0008 | 0.0006 | 0.0028 | 0.0132 | 0.0012 |
Vacuole | 0.021 | 0.0067 | 0.0098 | 0.006 | 0.007 | 0.0074 | 0.0088 | 0.0086 | 0.0075 | 0.0112 | 0.0082 | 0.0039 | 0.0051 | 0.0071 | 0.0095 | 0.009 | 0.0347 | 0.0036 |
Vacuole Periphery | 0.0029 | 0.0022 | 0.0023 | 0.0008 | 0.0011 | 0.0021 | 0.0027 | 0.0015 | 0.0021 | 0.0024 | 0.0022 | 0.0016 | 0.0023 | 0.0013 | 0.003 | 0.0028 | 0.0058 | 0.0017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.2593 | 11.3377 | 7.6482 | 11.8605 | 12.7066 | 12.6758 | 12.7662 | 12.8365 | 12.3915 | 14.3745 |
Translational Efficiency | 0.5576 | 0.8589 | 0.8923 | 0.6162 | 0.5806 | 0.9885 | 0.8873 | 0.8904 | 0.9307 | 0.6755 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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