Standard name
Human Ortholog
Description Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0.05 0.05 0.06 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.87 0.86 0.91 0.85 0.86 0.79 0.68 0.62 – 0.54 0.54 0.45 0.95 0.96 0.99 – – – 0.44 0.5 0.32 0.56 0.59 0.64
Endoplasmic Reticulum 0.07 0.09 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0.27 0.25 0.26 0.11 0.06 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.19 0.14 0.42 0.5 0.53 – 0.63 0.68 0.7 0 0 0 – – – 0.12 0.09 0.19 0.15 0.07 0.12
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 1 – 0 0 0 1 0 0 – – – 6 8 16 2 3 3
Bud 0 4 2 3 0 5 7 14 – 16 13 16 0 0 0 – – – 5 1 7 0 2 3
Bud Neck 0 0 1 0 0 0 1 2 – 0 0 1 1 1 1 – – – 2 5 6 0 1 2
Bud Site 0 0 0 0 0 0 3 2 – 2 1 2 0 0 0 – – – – – – – – –
Cell Periphery 1 2 2 2 6 5 9 4 – 4 3 1 3 4 2 – – – 2 3 7 0 1 1
Cytoplasm 192 192 269 250 288 289 412 368 – 284 183 182 89 134 132 – – – 117 119 99 23 38 60
Endoplasmic Reticulum 15 21 5 3 0 2 0 2 – 5 4 1 3 2 3 – – – 71 58 81 4 4 3
Endosome 1 0 3 5 9 2 1 6 – 1 0 1 0 0 0 – – – 4 5 5 0 1 1
Golgi 4 0 0 0 0 0 0 0 – 0 0 0 0 1 0 – – – 6 1 3 0 0 0
Mitochondria 8 7 15 56 46 155 306 312 – 332 232 287 0 0 0 – – – 31 21 58 6 4 10
Nucleus 0 0 2 1 0 1 0 2 – 3 2 2 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 0 0 0 1 – 2 1 1 0 0 0 – – – 0 0 2 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 – 1 1 3 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 2 0 1 0 0 2 – 0 0 0 0 0 0 – – – 0 1 1 0 0 0
SpindlePole 0 0 3 1 0 1 7 4 – 4 5 10 0 0 0 – – – 1 1 5 0 0 0
Vac/Vac Membrane 6 2 5 6 12 5 17 13 – 11 8 16 0 0 0 – – – 11 8 6 1 2 3
Unique Cell Count 220 222 294 294 335 368 606 591 524 340 408 94 139 134 265 239 312 43 66 94
Labelled Cell Count 227 230 311 328 362 465 763 733 665 453 523 97 142 138 265 239 312 43 66 94


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.6 4.4 4.1 4.1 3.3 3.0 3.2 – 2.8 2.9 2.9 5.7 5.3 5.3 4.0 4.1 4.5 6.2 6.2 5.8
Std Deviation (1e-4) 1.0 0.8 1.6 1.4 2.0 1.6 1.2 1.0 – 1.1 1.2 0.9 0.7 0.8 0.9 1.2 0.9 1.1 1.3 1.4 1.3
Intensity Change (Log2) – – – -0.13 -0.13 -0.43 -0.58 -0.45 – -0.65 -0.61 -0.61 0.36 0.26 0.27 -0.13 -0.11 0.02 0.48 0.48 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.095 2.4901 2.1532 2.5939 2.8991 2.7993 3.6689 5.1204 4.4678 2.7814 3.2012 4.7291 4.3133 4.5621 4.5996 4.7786 4.2854 4.8571
Actin 0.0343 0.0022 0.0294 0.0089 0.0221 0.0047 0.0054 0.0014 0.0041 0.001 0.0005 0.0008 0.0501 0.0006 0.003 0.0012 0.0136 0.0007
Bud 0.0011 0.0004 0.0019 0.0008 0.0063 0.0002 0.0001 0.0003 0.0001 0.0001 0 0 0.0008 0.0001 0.0002 0.0001 0.0002 0
Bud Neck 0.0121 0.0012 0.0027 0.0023 0.0031 0.0031 0.0003 0.0002 0.0015 0.001 0.0003 0.0021 0.0063 0.0004 0.0025 0.0008 0.0051 0.002
Bud Periphery 0.0021 0.0006 0.0042 0.0012 0.0075 0.0009 0.0002 0.0005 0.0003 0.0002 0.0001 0.0001 0.0019 0.0002 0.0003 0.0002 0.0003 0.0001
Bud Site 0.0081 0.0136 0.0214 0.0052 0.0698 0.0003 0.0005 0.0025 0.0017 0.0006 0 0 0.0035 0.0074 0.0088 0.0003 0.0008 0.0001
Cell Periphery 0.0031 0.0024 0.0019 0.002 0.0008 0.0015 0.0054 0.0027 0.0049 0.0047 0.001 0.0009 0.002 0.0047 0.0035 0.0028 0.001 0.001
Cytoplasm 0.1663 0.4866 0.3279 0.3462 0.3013 0.4201 0.2305 0.2857 0.2888 0.5793 0.3265 0.3041 0.2142 0.3428 0.2726 0.1969 0.2729 0.3789
Cytoplasmic Foci 0.06 0.012 0.0227 0.014 0.056 0.0125 0.0144 0.0118 0.0311 0.0057 0.0059 0.0054 0.0175 0.0057 0.0112 0.0035 0.0237 0.0071
Eisosomes 0.0007 0.0003 0.0003 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0008 0.0002 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.3043 0.2687 0.3271 0.4457 0.1654 0.3358 0.4986 0.3977 0.4916 0.217 0.5852 0.4791 0.4205 0.4508 0.5393 0.6912 0.504 0.4475
Endosome 0.0621 0.0161 0.0252 0.0389 0.0607 0.0153 0.0361 0.0134 0.0387 0.0044 0.0104 0.0099 0.0527 0.0062 0.0078 0.0093 0.0234 0.0066
Golgi 0.0258 0.0017 0.0066 0.0123 0.0268 0.0052 0.0182 0.0023 0.0083 0.0007 0.001 0.0009 0.0113 0.0008 0.0019 0.0011 0.0053 0.0004
Lipid Particles 0.0212 0.0054 0.0134 0.0143 0.0092 0.0039 0.0124 0.0069 0.0315 0.0036 0.0017 0.0029 0.0152 0.0132 0.0098 0.0063 0.0087 0.0028
Mitochondria 0.0328 0.0011 0.0034 0.0038 0.0284 0.0061 0.0092 0.0005 0.0012 0.0004 0.0005 0.0008 0.0038 0.0002 0.0003 0.0005 0.0008 0.0003
None 0.1431 0.1563 0.1692 0.0718 0.1779 0.1499 0.1227 0.2584 0.0681 0.1692 0.0557 0.1798 0.1353 0.1495 0.1228 0.0699 0.1207 0.1394
Nuclear Periphery 0.0234 0.0104 0.013 0.014 0.0242 0.0154 0.0205 0.008 0.0096 0.0057 0.006 0.0066 0.0194 0.0082 0.007 0.0093 0.0074 0.0065
Nucleolus 0.006 0.0006 0.0019 0.0004 0.0017 0.0003 0.0004 0.0003 0.0003 0.0001 0 0.0001 0.0026 0.0002 0.0008 0.0001 0.0004 0.0001
Nucleus 0.0378 0.0095 0.0067 0.005 0.0093 0.0156 0.0123 0.0019 0.0014 0.0024 0.0015 0.0018 0.0069 0.0026 0.0014 0.0017 0.0021 0.0021
Peroxisomes 0.0261 0.0013 0.0097 0.002 0.0117 0.0021 0.0034 0.0003 0.0037 0.0001 0 0 0.0028 0.0001 0.0011 0.0001 0.0017 0.0001
Punctate Nuclear 0.0167 0.0025 0.0047 0.0024 0.0048 0.0026 0.0016 0.0015 0.007 0.0005 0.0003 0.0018 0.0186 0.0006 0.0014 0.0003 0.0034 0.0012
Vacuole 0.0095 0.0067 0.0059 0.0073 0.0103 0.0036 0.0064 0.0034 0.0051 0.0031 0.003 0.0026 0.0121 0.0054 0.0039 0.0038 0.0037 0.0029
Vacuole Periphery 0.0034 0.0005 0.001 0.0012 0.0023 0.0007 0.0013 0.0004 0.0008 0.0002 0.0003 0.0003 0.0018 0.0003 0.0003 0.0004 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.879 14.4583 13.9075 15.2572 13.2033 16.7634 15.7016 14.9827 15.6417 16.6561
Translational Efficiency 0.5777 0.4866 0.4529 0.3874 0.3609 0.514 0.4543 0.4406 0.501 0.3507

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
125 539 1443 1461 109 1884 2061 2027 234 2423 3504 3488

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 888.99 702.55 917.10 803.31 689.24 755.65 835.69 855.61 795.94 743.84 869.22 833.70
Standard Deviation 150.96 93.55 134.06 111.31 75.81 104.52 115.96 113.38 157.42 104.54 130.06 115.44
Intensity Change Log 2 — -0.339566 0.044912 -0.146210 — 0.132712 0.277961 0.311947 — -0.114119 0.151346 0.071937

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001296 0.001040 0.001208 0.001254 0.000902 0.000963 0.000666 0.001058 0.001113 0.000980 0.000889 0.001140
Bud Neck 0.009006 0.036057 0.005647 0.011203 0.008640 0.038363 0.010981 0.010608 0.008835 0.037850 0.008784 0.010857
Bud Site 0.048133 0.017252 0.011522 0.037717 0.005562 0.018020 0.017431 0.024599 0.028303 0.017849 0.014998 0.030093
Cell Periphery 0.000323 0.000310 0.000548 0.000293 0.000854 0.000284 0.000697 0.000493 0.000571 0.000289 0.000635 0.000409
Cytoplasm 0.243980* 0.338372* 0.390647* 0.451086* 0.349342* 0.373558* 0.423354* 0.473659* 0.293059* 0.365731* 0.409885* 0.464204*
Cytoplasmic Foci 0.084631 0.294182* 0.034546 0.045203 0.321195* 0.252310* 0.022798 0.040915 0.194825* 0.261625* 0.027636 0.042711
Eisosomes 0.000119 0.000475 0.000082 0.000187 0.001416 0.000376 0.000138 0.000197 0.000723 0.000398 0.000115 0.000193
Endoplasmic Reticulum 0.007301 0.009279 0.213140* 0.010867 0.026735 0.008524 0.092766 0.032197 0.016353 0.008692 0.142338* 0.023263
Endosome 0.031011 0.022396 0.008992 0.012320 0.015044 0.026529 0.004799 0.007234 0.023573 0.025610 0.006526 0.009364
Golgi 0.011944 0.005168 0.000685 0.004557 0.006938 0.008280 0.000325 0.001441 0.009612 0.007588 0.000473 0.002746
Lipid Particles 0.003183 0.024834 0.011825 0.006215 0.072562 0.024320 0.007732 0.008048 0.035501 0.024434 0.009418 0.007280
Mitochondria 0.001121 0.006674 0.000578 0.003870 0.006869 0.016941 0.000988 0.002243 0.003799 0.014657 0.000819 0.002925
Mitotic Spindle 0.007430 0.001036 0.001179 0.038200 0.002497 0.007343 0.002769 0.014536 0.005132 0.005940 0.002114 0.024448
None 0.000542 0.003382 0.001815 0.007868 0.001074 0.003362 0.002622 0.003627 0.000790 0.003367 0.002290 0.005404
Nuclear Periphery 0.000788 0.001588 0.017661 0.002454 0.002763 0.001420 0.010089 0.003747 0.001708 0.001457 0.013207 0.003205
Nuclear Periphery Foci 0.002040 0.002482 0.068010 0.004660 0.002144 0.000798 0.015589 0.006438 0.002088 0.001172 0.037177 0.005693
Nucleolus 0.000371 0.001574 0.000384 0.000929 0.000780 0.001652 0.000351 0.000636 0.000561 0.001634 0.000365 0.000759
Nucleus 0.129834 0.113307 0.163475* 0.192661* 0.112515 0.113406 0.314829* 0.229806* 0.121767 0.113384 0.252499* 0.214247*
Peroxisomes 0.002102 0.024544 0.000519 0.001698 0.014485 0.015892 0.000689 0.001369 0.007870 0.017817 0.000619 0.001507
Vacuole 0.405596* 0.094425 0.067133 0.163991 0.046296 0.084732 0.069919 0.135542 0.238230* 0.086888 0.068772 0.147458
Vacuole Periphery 0.009248 0.001623 0.000405 0.002766 0.001388 0.002927 0.000469 0.001608 0.005587 0.002637 0.000442 0.002093

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.09 -0.35 0.58 0.68 1.14 -0.08 0.48 0.15 0.26 -0.82 0.05 0.24 0.55 0.44 0.54
Bud Neck -4.95 0.59 -0.11 6.73 -2.25 -9.82 -0.88 -0.73 13.18 0.39 -9.95 -0.14 -0.50 15.01 -1.01
Bud Site 2.02 2.63 1.37 -1.68 -3.76 -3.44 -3.05 -3.74 -0.51 -1.03 0.81 1.39 0.22 -1.55 -3.25
Cell Periphery -0.37 -4.07 1.26 2.56 8.31 1.55 0.57 1.31 -2.14 3.62 1.48 -0.35 1.41 -0.46 6.50
Cytoplasm -3.79 -6.27 -6.86 -4.96 -1.27 -0.97 -2.50 -3.27 -6.41 -2.21 -4.17 -6.36 -7.32 -7.33 -2.47
Cytoplasmic Foci -19.43 3.52 3.53 27.98 0.04 2.96 13.05 12.44 43.42 -7.30 -5.30 10.93 10.36 51.70 -5.37
Eisosomes -3.29 2.18 -0.38 2.93 -1.02 1.61 1.98 1.95 5.75 -1.02 1.06 2.00 1.89 6.10 -1.41
Endoplasmic Reticulum -1.26 -26.93 -0.61 0.86 27.41 1.95 -5.40 0.53 -5.19 12.17 1.44 -19.48 -0.05 -3.89 27.35
Endosome -0.90 2.38 2.70 7.55 1.54 -4.56 4.87 4.31 15.06 -1.97 -3.31 4.45 4.36 16.26 -0.20
Golgi 0.37 1.84 1.29 2.40 -2.30 -0.53 2.83 2.54 10.54 -2.00 -0.47 3.13 2.58 9.67 -3.02
Lipid Particles -8.10 -11.76 -1.88 7.54 9.02 4.41 6.01 6.11 12.82 1.67 1.93 4.72 5.41 15.29 7.29
Mitochondria -5.48 0.27 -2.62 3.88 -3.37 -2.89 1.86 1.62 11.36 -1.89 -6.03 1.84 1.09 11.86 -3.64
Mitotic Spindle 1.92 1.82 -5.60 -8.03 -7.99 -1.87 -0.30 -4.16 -3.18 -5.65 -1.10 0.94 -6.79 -6.80 -9.69
None -2.62 -4.28 -4.75 -1.42 -3.57 -3.15 -3.20 -3.75 0.29 -1.27 -4.26 -5.10 -6.24 -0.89 -3.58
Nuclear Periphery -1.19 -12.99 -8.03 -3.34 11.22 1.57 -5.70 -1.08 -4.82 6.02 0.89 -13.32 -3.50 -6.17 12.06
Nuclear Periphery Foci -0.78 -21.74 -2.35 -1.46 20.98 1.72 -9.60 -3.15 -7.68 8.00 2.19 -22.88 -4.24 -7.85 21.27
Nucleolus -5.92 -1.45 -3.28 2.90 -2.53 -3.67 2.74 0.33 4.42 -3.62 -6.45 1.62 -2.19 4.92 -4.25
Nucleus 1.05 -1.54 -4.06 -13.02 -7.01 -0.12 -16.00 -10.80 -23.39 9.54 0.88 -10.69 -9.12 -26.57 3.73
Peroxisomes -7.68 2.77 1.24 7.87 -2.63 -0.44 4.41 4.25 14.27 -2.51 -5.60 4.53 4.21 16.03 -3.55
Vacuole 12.76 13.75 7.45 -16.97 -23.28 -4.94 -4.91 -15.48 -17.82 -17.47 8.72 9.09 2.79 -25.84 -28.48
Vacuole Periphery 1.20 1.64 1.26 0.43 -2.41 -3.19 3.00 0.79 3.76 -1.78 0.43 1.93 1.38 3.64 -2.92
Standard name
Human Ortholog
Description Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Ecm3

Ecm3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available