Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0.05 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.07 0.11 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0.08 0 0.22 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Golgi 0 0 0.1 0.26 0.22 0 0 0 0 0 0 0 0.17 0.32 0.44 0.47 0.39 0.15 0 0.08 0.05 0.06 0.06 0.07
Mitochondria 0.96 0.92 0.85 0.91 0.81 0.82 0.83 0.83 0.88 0.87 0.9 0.92 0.84 0.73 0.65 0.13 0.07 0.13 0.9 0.76 0.8 0.75 0.69 0.69
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.08 0 0.05 0 0 0.13 0.11 0.07 0.09 0.12 0.1 0.08 0.09 0.06 0 0 0.07 0 0 0 0 0 0 0
Nucleolus 0.09 0 0.06 0 0.05 0.08 0.14 0.08 0.05 0.08 0.07 0.05 0 0 0 0 0 0.17 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.07 0 0 0.05 0.07 0.09 0.1 0.09
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.11 0 0 0.08 0.07 0 0.06 0 0.05 0 0.05 0.13 0.11 0.16 0.2 0.11 0.17 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 0 4 0 0 1 0 2 2 4 4 3 2 2 5 0 5 21 13
Bud 0 0 0 4 4 1 9 3 6 7 3 5 3 0 2 1 2 1 0 1 0 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 1 1 2 4 0 2 2 0 0 0 0 1 1 – – – – – –
Cell Periphery 3 5 1 5 4 1 4 2 0 2 3 1 6 2 15 13 2 5 0 0 0 0 0 0
Cytoplasm 3 4 2 5 7 16 16 16 8 12 9 2 3 6 9 11 1 10 0 0 0 0 0 0
Endoplasmic Reticulum 0 1 1 1 3 2 1 0 0 3 1 2 4 3 2 5 0 2 0 0 0 0 1 0
Endosome 0 0 1 3 6 8 7 7 0 3 0 0 8 18 16 3 0 3 4 1 2 6 12 17
Golgi 2 5 9 34 47 11 5 5 2 3 2 4 54 132 181 61 11 7 4 10 5 13 26 27
Mitochondria 166 123 80 118 174 214 301 234 192 259 228 257 269 307 271 17 2 6 147 103 96 174 294 295
Nucleus 0 0 3 0 0 0 2 2 2 3 2 0 0 0 3 1 0 1 0 0 0 0 1 0
Nuclear Periphery 14 3 5 5 9 35 40 21 19 37 26 23 28 26 15 2 2 1 0 0 1 1 2 3
Nucleolus 16 3 6 1 10 20 49 22 12 23 18 14 14 11 8 3 1 8 0 0 0 0 4 4
Peroxisomes 0 0 1 1 7 2 6 6 1 3 1 3 11 16 29 7 2 1 4 6 7 20 43 37
SpindlePole 1 0 0 0 3 2 6 2 2 4 3 2 4 1 5 3 2 1 0 0 0 3 2 4
Vac/Vac Membrane 13 15 4 5 18 18 16 16 9 15 9 14 43 47 68 26 3 8 0 3 1 5 7 5
Unique Cell Count 173 134 94 130 214 260 362 281 219 299 253 278 322 418 415 130 28 46 164 136 120 233 424 425
Labelled Cell Count 218 159 113 183 293 331 463 342 257 374 308 329 449 571 628 157 32 57 164 136 120 233 424 425


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 13.4 9.4 10.5 10.2 9.4 8.8 9.2 8.1 8.4 8.0 8.7 10.7 11.3 11.1 12.0 12.4 11.4 10.8 11.9 11.9
Std Deviation (1e-4) 1.7 3.0 2.3 2.2 1.8 2.5 2.2 2.2 2.1 2.1 2.2 2.3 1.9 2.0 1.8 2.9 3.0 3.6 2.3 2.3 2.4
Intensity Change (Log2) – – – 0.15 0.12 -0.01 -0.1 -0.03 -0.21 -0.16 -0.23 -0.11 0.19 0.27 0.24 0.35 0.4 0.28 0.2 0.33 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.7 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 2.0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.1 2.6 -1.9 0 0 0 0 0 0 1.7 4.3 6.2 6.0 0 1.0
Mitochondria 1.3 -0.8 -0.6 -0.5 -0.4 0.6 0.4 1.3 2.1 -0.4 -2.4 -3.7 -10.7 -7.7 -8.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.1 1.7 0.7 1.0 1.9 1.4 0.9 1.1 0.3 0 0 0 0
Nucleolus 0 0 0.4 1.9 0.5 -0.3 0.4 0.2 -0.5 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 2.2 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.3 0.9 0 0.5 0 0 0 0 2.5 2.0 3.0 3.4 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.6475 7.5314 7.4188 6.251 8.0248 6.9266 9.5383 8.1439 8.5615 8.1686 8.1539 8.285 10.5435 9.8017 9.9138 9.103 9.1107 9.8948
Actin 0.007 0.0074 0.0016 0.0017 0.0002 0.0006 0.0042 0.0037 0.0009 0.0006 0.0004 0.0027 0.0188 0.01 0.0062 0.0007 0.0027 0.0114
Bud 0.0003 0.0005 0.0001 0 0 0 0.0001 0.0002 0 0 0 0 0.0001 0.0021 0.0001 0 0.0007 0
Bud Neck 0.0006 0.0001 0.0001 0 0 0 0.0002 0.0001 0 0 0 0 0.0006 0.0001 0 0 0 0.0001
Bud Periphery 0.0007 0.0007 0.0002 0.0001 0.0001 0.0001 0.0002 0.0005 0.0001 0.0001 0.0001 0.0001 0.0003 0.0028 0.0004 0.0001 0.0019 0.0001
Bud Site 0.0003 0.0045 0.0002 0 0 0 0.0002 0.0004 0.0001 0.0001 0 0.0001 0.0003 0.0015 0.0001 0.0001 0.0002 0.0001
Cell Periphery 0.0002 0.0003 0.0002 0 0.0001 0.0001 0.0001 0.0001 0 0 0 0 0.0002 0.0002 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.0003 0.0001 0.0002 0 0 0 0.0012 0 0 0 0 0 0 0 0 0 0 0
Cytoplasmic Foci 0.0013 0.0041 0.0007 0.0005 0.0003 0.0005 0.0011 0.0024 0.0011 0.0012 0.0004 0.001 0.0011 0.0013 0.0017 0.0004 0.002 0.0002
Eisosomes 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0012 0.0003 0.0004 0.0002 0.0003 0.0002
Endoplasmic Reticulum 0.0012 0.0001 0.0002 0 0 0.0001 0.0006 0.0001 0 0 0 0 0.0001 0.0001 0.0001 0.0001 0 0
Endosome 0.0148 0.0132 0.0047 0.001 0.0037 0.0051 0.0041 0.0052 0.0015 0.0027 0.0015 0.002 0.0029 0.0076 0.0064 0.0013 0.0016 0.0013
Golgi 0.0142 0.0342 0.0152 0.0301 0.0044 0.0237 0.0051 0.0151 0.008 0.0038 0.0485 0.0115 0.0113 0.0229 0.0263 0.003 0.0043 0.0114
Lipid Particles 0.0104 0.0037 0.0112 0.0001 0.0016 0.003 0.0122 0.0072 0.0046 0.0037 0.0002 0.0075 0.0184 0.0057 0.006 0.0137 0.0352 0.0079
Mitochondria 0.9276 0.9113 0.9442 0.9657 0.9828 0.9576 0.954 0.9505 0.9775 0.9839 0.9468 0.9653 0.9277 0.9254 0.9307 0.9741 0.9314 0.9589
None 0.0002 0 0.0001 0 0 0 0.0008 0 0 0 0 0 0.0001 0 0 0 0 0
Nuclear Periphery 0.0013 0.0002 0.0041 0 0 0.0001 0.0012 0.0001 0 0 0 0.0001 0.0002 0.0003 0.0001 0.0001 0 0.0001
Nucleolus 0.0002 0.0016 0.0004 0 0 0 0.0003 0.0002 0 0 0 0 0.0001 0.0006 0.0002 0.0002 0.0001 0.0002
Nucleus 0.0003 0.0003 0.0025 0 0 0 0.0006 0.0001 0 0 0 0 0.0001 0.0001 0.0001 0.0001 0 0.0001
Peroxisomes 0.0038 0.0079 0.0021 0.0002 0.0007 0.0066 0.0042 0.0093 0.0045 0.0026 0.001 0.0047 0.0081 0.0103 0.0032 0.0029 0.0179 0.0013
Punctate Nuclear 0.0001 0 0.0001 0 0 0 0.0004 0 0 0 0 0 0.0001 0 0 0 0 0
Vacuole 0.0045 0.0029 0.0042 0.0001 0.0013 0.0003 0.004 0.0013 0.0003 0.0003 0.0001 0.0003 0.0011 0.0028 0.005 0.0005 0.0005 0.0006
Vacuole Periphery 0.0103 0.0066 0.0076 0.0004 0.0047 0.0022 0.005 0.0031 0.001 0.0007 0.0008 0.0045 0.0071 0.0057 0.0125 0.0025 0.001 0.006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.1063 25.7674 22.1798 30.6095 17.1913 20.7422 37.5169 40.7955 38.9183 37.1468
Translational Efficiency 3.1733 1.4244 1.6266 1.276 2.0441 2.9105 1.4778 1.5394 1.6744 1.5437

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1610 567 1339 1761 1861 2170 619 428 3471 2737 1958 2189

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 884.56 1025.51 1244.35 1268.91 1015.66 1108.31 1111.17 1279.69 954.85 1091.16 1202.25 1271.02
Standard Deviation 164.38 220.00 219.22 237.90 173.98 190.32 222.90 259.39 181.76 199.68 228.92 242.29
Intensity Change Log 2 — 0.213310 0.492360 0.520558 — 0.125944 0.129662 0.333377 — 0.167272 0.309879 0.423539

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002275 0.005411 0.004371 0.004556 0.001866 0.002009 0.003099 0.005367 0.002056 0.002713 0.003969 0.004714
Bud Neck 0.002019 0.002791 0.002724 0.002552 0.000574 0.000327 0.019492 0.005449 0.001244 0.000838 0.008025 0.003118
Bud Site 0.003329 0.006745 0.002857 0.003781 0.000372 0.001151 0.033045 0.008738 0.001743 0.002310 0.012401 0.004751
Cell Periphery 0.000548 0.000569 0.000134 0.000327 0.000078 0.000081 0.002027 0.000260 0.000296 0.000182 0.000733 0.000314
Cytoplasm 0.000573 0.000760 0.000553 0.000633 0.000152 0.000342 0.008536 0.001120 0.000347 0.000429 0.003076 0.000728
Cytoplasmic Foci 0.008188 0.023285 0.021015 0.011700 0.011596 0.010110 0.030775 0.033020 0.010015 0.012839 0.024101 0.015869
Eisosomes 0.000131 0.000452 0.000160 0.000189 0.000085 0.000095 0.000161 0.000203 0.000106 0.000169 0.000161 0.000191
Endoplasmic Reticulum 0.000351 0.000373 0.000312 0.000155 0.000090 0.000084 0.001143 0.000356 0.000211 0.000144 0.000575 0.000194
Endosome 0.015661 0.015741 0.010697 0.008826 0.006872 0.005747 0.075789 0.030155 0.010949 0.007817 0.031275 0.012996
Golgi 0.011846 0.037799 0.031937 0.020342 0.015879 0.024162 0.028393 0.053031 0.014009 0.026987 0.030816 0.026733
Lipid Particles 0.008100 0.009453 0.004959 0.001871 0.006075 0.002580 0.016227 0.005292 0.007014 0.004004 0.008521 0.002540
Mitochondria 0.910503* 0.840904* 0.872138* 0.911592* 0.939275* 0.938421* 0.659714* 0.781144* 0.925929* 0.918219* 0.804983* 0.886086*
Mitotic Spindle 0.000853 0.000746 0.008424 0.004942 0.001337 0.000837 0.019182 0.014465 0.001112 0.000818 0.011825 0.006804
None 0.000575 0.001243 0.000949 0.000835 0.000527 0.000415 0.001450 0.001039 0.000549 0.000586 0.001107 0.000875
Nuclear Periphery 0.000075 0.000143 0.000766 0.000295 0.000132 0.000083 0.002187 0.001034 0.000106 0.000095 0.001215 0.000439
Nuclear Periphery Foci 0.000217 0.000428 0.000542 0.000289 0.000149 0.000090 0.003692 0.000903 0.000180 0.000160 0.001538 0.000409
Nucleolus 0.000199 0.001020 0.000431 0.000289 0.000176 0.000037 0.005049 0.001327 0.000187 0.000241 0.001891 0.000492
Nucleus 0.000134 0.000298 0.000141 0.000090 0.000120 0.000020 0.003273 0.000594 0.000126 0.000078 0.001132 0.000189
Peroxisomes 0.027757 0.041883 0.015953 0.009230 0.010256 0.008403 0.038824 0.021835 0.018374 0.015339 0.023184 0.011695
Vacuole 0.003682 0.006515 0.008772 0.008358 0.001823 0.002156 0.031700 0.016855 0.002686 0.003059 0.016021 0.010020
Vacuole Periphery 0.002987 0.003440 0.012163 0.009149 0.002566 0.002850 0.016244 0.017814 0.002761 0.002972 0.013453 0.010843

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.05 -2.83 -2.35 0.64 -0.15 -0.21 -1.38 -1.99 -1.94 -1.23 -1.28 -3.22 -3.09 -2.25 -0.77
Bud Neck -1.01 -1.23 -1.08 0.29 0.30 1.38 -6.85 -5.41 -5.77 4.83 1.55 -7.13 -5.14 -7.06 5.01
Bud Site -1.66 0.54 -0.60 1.38 -1.20 -2.25 -7.61 -4.26 -3.82 5.12 -1.00 -7.29 -4.21 -3.07 4.78
Cell Periphery -0.11 3.28 0.99 0.98 -1.05 -0.17 -3.03 -2.31 -2.27 2.72 1.66 -2.06 -0.12 -0.87 1.65
Cytoplasm -0.78 0.10 -0.43 0.47 -0.59 -2.22 -3.69 -4.33 -3.24 3.25 -0.84 -3.74 -3.20 -2.34 3.18
Cytoplasmic Foci -5.12 -7.12 -2.57 3.79 4.76 1.10 -6.57 -5.37 -5.82 -0.45 -2.50 -9.07 -4.32 -2.09 4.49
Eisosomes -6.03 -1.51 -1.72 4.36 -0.83 -1.07 -5.33 -2.36 -2.18 -0.81 -4.50 -4.21 -3.07 -0.78 -1.07
Endoplasmic Reticulum -0.20 0.46 2.82 2.43 3.08 0.38 -8.34 -4.73 -4.84 5.74 1.79 -6.02 0.43 -1.87 6.90
Endosome -0.03 2.71 3.79 2.74 1.31 1.27 -12.07 -5.82 -6.15 6.60 3.05 -9.28 -1.53 -4.07 7.85
Golgi -6.37 -7.48 -3.92 3.97 3.68 -3.65 -3.98 -5.89 -4.50 -3.64 -6.93 -7.96 -5.89 0.10 1.48
Lipid Particles -0.61 2.23 4.84 4.06 4.73 3.57 -3.73 0.55 -2.37 3.92 3.34 -1.37 5.48 2.64 6.12
Mitochondria 5.32 4.58 -0.08 -5.40 -4.73 0.17 18.28 10.00 9.99 -5.73 1.55 16.01 6.64 5.07 -9.45
Mitotic Spindle 0.48 -6.18 -4.88 -5.07 2.35 1.09 -6.22 -4.66 -4.86 1.23 0.98 -8.59 -6.42 -6.79 3.35
None -2.13 -5.34 -3.84 1.30 1.69 2.49 -2.96 -3.98 -5.06 1.28 -0.51 -5.12 -5.99 -3.63 2.09
Nuclear Periphery -2.60 -7.63 -6.08 -3.56 4.86 2.56 -2.71 -5.23 -5.52 1.48 0.74 -4.47 -7.39 -7.56 3.08
Nuclear Periphery Foci -1.84 -4.11 -1.12 1.18 3.12 1.80 -5.44 -4.77 -5.13 4.24 0.53 -6.29 -4.18 -4.39 5.17
Nucleolus -1.76 -1.64 -1.16 1.57 1.06 1.97 -3.86 -1.59 -1.79 2.58 -0.52 -4.12 -2.01 -1.45 3.22
Nucleus -1.23 -0.16 1.01 1.57 1.21 1.27 -4.33 -1.82 -2.29 3.48 0.92 -4.25 -0.98 -1.97 3.94
Peroxisomes -2.25 3.59 6.54 5.59 2.92 1.15 -7.12 -2.93 -3.47 3.18 1.52 -2.11 3.65 1.94 5.28
Vacuole -1.70 -4.43 -4.60 -1.07 0.26 -0.73 -11.58 -6.76 -6.57 4.36 -0.72 -11.81 -8.45 -7.54 4.35
Vacuole Periphery -0.67 -7.48 -6.08 -5.14 2.00 -0.71 -8.96 -5.69 -5.58 -0.52 -0.61 -11.16 -8.51 -8.16 1.98
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Pet123

Pet123


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pet123-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available